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The map label for this gene is murE [H]

Identifier: 119897170

GI number: 119897170

Start: 939385

End: 940890

Strand: Direct

Name: murE [H]

Synonym: azo0879

Alternate gene names: 119897170

Gene position: 939385-940890 (Clockwise)

Preceding gene: 119897169

Following gene: 119897171

Centisome position: 21.47

GC content: 70.39

Gene sequence:

>1506_bases
GTGAGCAACCCTGCCTTCGTGCTGCTCGATCAGCTCCGCGCCAGCGGCGTCCGGCCCGCCGGCATCACGGCCGATTCGCG
TCGTGTCGGGCCGGGGGCCGTGTTCGCGGCGTGGCCGGGGCATGTCACCGACGGCCGTCGTTACATCGCCTCCGCCATCG
AGCGCGGCGCCGCCGCCGTGTTGTGGGAAGAGGGCGACGGCTTTCACCCGGGAGAACTTCCGGTTCCAGGGTTCGCGGTG
CCCAGGCTGCGCGAACTCGCGGGTGCGCTTGCCCACGAGATCTATGACCGGCCCTCCGCGCGGCTGTGGATGGCCGGCGT
GACCGGCACCAACGGCAAGACCACGGTCACGCAGTGGCTCGCGCGCGCGCTGGGCGAACTCGGCAGCCGCTGCGGCATCG
TCGGTACCCTCGGAAGCGGGTTTCCGGACCAGCTCAGCGCCAGTCTCAACACCACGCCCGATGCGCTCGAACTGCATGCC
ACGCTCGCGCGCCTGCTGGGCGAAGGCGCAGCGGCAGCGGCCATGGAAGTGTCGTCCATCGGACTCGACCAAGGGCGGGT
GAACGGCGTCGAGTTCGACGTCGCGGTGTTCACCAACCTTACCCGCGACCATCTCGATTACCACCGCACGATGGACGCCT
ATGCCGAAGCCAAGGCGCGTCTTTTCGCGCTGCCGGGCATCAGCCGCGCCGTCATCAATCTGGACGACGCCTTCGGCCTG
ACGCAGGCCCGCCGCCTCGTCGCCGCCGGGCAGGTGGATGTGATCGGCTACACCTGCGTCGCCTCCAACGCGGATGCGGT
GCCCGGCGCCCGCGTGCTGGTGGCGGACCGGCTCCAGGCCTCGCCCTCGGGCCTGCAGTTCTCGCTGCACTGGGCGGGGC
GCCAGTCCGACCTGCAGGTGCGCATGGTCGCCCCGTTCAATGTCTCCAATCTGCTCGCGGTGATCGGCGCGCTCGTCATG
CGCGGGGTCGCGGTAGAGGAAGCGCTGGCCGTGGTCGGCCGCCTCAATCCGCCGGAAGGTCGCATGCAACTGGTGGGCGG
GATTGGAGAGCCGCTGATCGTGATCGACTACGCGCACACGCCGGATGCGCTCGCCAAGGTGCTGGAGGCGGTGCGCGGCA
CCGTGCGCAGCCGCGGCGGCCGCCTCGTGTGCGTGTTCGGCTGCGGCGGCGATCGCGATCCCGGCAAGCGCCCCCTGATG
GGAGAAGTCGCCCGCCAACTGGCCGACCGCGTCGTGGTCACCAGCGACAACCCGCGCAGCGAAGACCCGCGCAAGATCAT
CGACGCCATTGCCGCCGGGGCGGGTGCGTCGGCCGACTGCATCGTGGACCGTGCCGAAGCGATCCGCATCGCGGTCGGCG
AGGCCGGGCCCGACGACGTCGTCGTGCTGGCCGGCAAGGGCCACGAACCATATCAGGAGATCCACGGTCAGCGTCTGCCG
TTCTCCGACGTCGAGCAGGCGAAGGCGGCGCTGGCAGTGTGGAACGACAGGAGAACGACGGCATGA

Upstream 100 bases:

>100_bases
CGCTGCGCACCCTCGGCGTGGCACCGGATGCGCCGCTGACGCCGCTGCAGCTGGCCCGCAAGCCCAACGAACCCGCGCCC
GCGGCGGTCCGGGAGAGCAT

Downstream 100 bases:

>100_bases
TGAGCCTGCTCGACGCCAGCCGCGCGCTGTCCGCACATCACGCGGTGGCGCAAGGCGCGGTGCGCTTTTCCAGTGTCGGC
ACCGACAGCCGCGGCATCAT

Product: UDP-N-acetylmuramoylalanyl-D-glutamate-2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 501; Mature: 500

Protein sequence:

>501_residues
MSNPAFVLLDQLRASGVRPAGITADSRRVGPGAVFAAWPGHVTDGRRYIASAIERGAAAVLWEEGDGFHPGELPVPGFAV
PRLRELAGALAHEIYDRPSARLWMAGVTGTNGKTTVTQWLARALGELGSRCGIVGTLGSGFPDQLSASLNTTPDALELHA
TLARLLGEGAAAAAMEVSSIGLDQGRVNGVEFDVAVFTNLTRDHLDYHRTMDAYAEAKARLFALPGISRAVINLDDAFGL
TQARRLVAAGQVDVIGYTCVASNADAVPGARVLVADRLQASPSGLQFSLHWAGRQSDLQVRMVAPFNVSNLLAVIGALVM
RGVAVEEALAVVGRLNPPEGRMQLVGGIGEPLIVIDYAHTPDALAKVLEAVRGTVRSRGGRLVCVFGCGGDRDPGKRPLM
GEVARQLADRVVVTSDNPRSEDPRKIIDAIAAGAGASADCIVDRAEAIRIAVGEAGPDDVVVLAGKGHEPYQEIHGQRLP
FSDVEQAKAALAVWNDRRTTA

Sequences:

>Translated_501_residues
MSNPAFVLLDQLRASGVRPAGITADSRRVGPGAVFAAWPGHVTDGRRYIASAIERGAAAVLWEEGDGFHPGELPVPGFAV
PRLRELAGALAHEIYDRPSARLWMAGVTGTNGKTTVTQWLARALGELGSRCGIVGTLGSGFPDQLSASLNTTPDALELHA
TLARLLGEGAAAAAMEVSSIGLDQGRVNGVEFDVAVFTNLTRDHLDYHRTMDAYAEAKARLFALPGISRAVINLDDAFGL
TQARRLVAAGQVDVIGYTCVASNADAVPGARVLVADRLQASPSGLQFSLHWAGRQSDLQVRMVAPFNVSNLLAVIGALVM
RGVAVEEALAVVGRLNPPEGRMQLVGGIGEPLIVIDYAHTPDALAKVLEAVRGTVRSRGGRLVCVFGCGGDRDPGKRPLM
GEVARQLADRVVVTSDNPRSEDPRKIIDAIAAGAGASADCIVDRAEAIRIAVGEAGPDDVVVLAGKGHEPYQEIHGQRLP
FSDVEQAKAALAVWNDRRTTA
>Mature_500_residues
SNPAFVLLDQLRASGVRPAGITADSRRVGPGAVFAAWPGHVTDGRRYIASAIERGAAAVLWEEGDGFHPGELPVPGFAVP
RLRELAGALAHEIYDRPSARLWMAGVTGTNGKTTVTQWLARALGELGSRCGIVGTLGSGFPDQLSASLNTTPDALELHAT
LARLLGEGAAAAAMEVSSIGLDQGRVNGVEFDVAVFTNLTRDHLDYHRTMDAYAEAKARLFALPGISRAVINLDDAFGLT
QARRLVAAGQVDVIGYTCVASNADAVPGARVLVADRLQASPSGLQFSLHWAGRQSDLQVRMVAPFNVSNLLAVIGALVMR
GVAVEEALAVVGRLNPPEGRMQLVGGIGEPLIVIDYAHTPDALAKVLEAVRGTVRSRGGRLVCVFGCGGDRDPGKRPLMG
EVARQLADRVVVTSDNPRSEDPRKIIDAIAAGAGASADCIVDRAEAIRIAVGEAGPDDVVVLAGKGHEPYQEIHGQRLPF
SDVEQAKAALAVWNDRRTTA

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=473, Percent_Identity=43.5517970401691, Blast_Score=301, Evalue=4e-83,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 52685; Mature: 52553

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNPAFVLLDQLRASGVRPAGITADSRRVGPGAVFAAWPGHVTDGRRYIASAIERGAAAV
CCCCCEEEEEHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCEEE
LWEEGDGFHPGELPVPGFAVPRLRELAGALAHEIYDRPSARLWMAGVTGTNGKTTVTQWL
EEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHH
ARALGELGSRCGIVGTLGSGFPDQLSASLNTTPDALELHATLARLLGEGAAAAAMEVSSI
HHHHHHHHHHCCEEECCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHC
GLDQGRVNGVEFDVAVFTNLTRDHLDYHRTMDAYAEAKARLFALPGISRAVINLDDAFGL
CCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCEEEEECCHHCCH
TQARRLVAAGQVDVIGYTCVASNADAVPGARVLVADRLQASPSGLQFSLHWAGRQSDLQV
HHHHHHHHCCCEEEEEEEEEECCCCCCCCCEEEEEHHHCCCCCCCEEEEEECCCCCCEEE
RMVAPFNVSNLLAVIGALVMRGVAVEEALAVVGRLNPPEGRMQLVGGIGEPLIVIDYAHT
EEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHEEECCCCCCEEEEEECCC
PDALAKVLEAVRGTVRSRGGRLVCVFGCGGDRDPGKRPLMGEVARQLADRVVVTSDNPRS
HHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHCEEEEECCCCCC
EDPRKIIDAIAAGAGASADCIVDRAEAIRIAVGEAGPDDVVVLAGKGHEPYQEIHGQRLP
CCHHHHHHHHHCCCCCCCHHHHHHCCEEEEEECCCCCCCEEEEECCCCCCHHHHCCCCCC
FSDVEQAKAALAVWNDRRTTA
CHHHHHHHHHHHEECCCCCCC
>Mature Secondary Structure 
SNPAFVLLDQLRASGVRPAGITADSRRVGPGAVFAAWPGHVTDGRRYIASAIERGAAAV
CCCCEEEEEHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCEEE
LWEEGDGFHPGELPVPGFAVPRLRELAGALAHEIYDRPSARLWMAGVTGTNGKTTVTQWL
EEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHH
ARALGELGSRCGIVGTLGSGFPDQLSASLNTTPDALELHATLARLLGEGAAAAAMEVSSI
HHHHHHHHHHCCEEECCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHC
GLDQGRVNGVEFDVAVFTNLTRDHLDYHRTMDAYAEAKARLFALPGISRAVINLDDAFGL
CCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCEEEEECCHHCCH
TQARRLVAAGQVDVIGYTCVASNADAVPGARVLVADRLQASPSGLQFSLHWAGRQSDLQV
HHHHHHHHCCCEEEEEEEEEECCCCCCCCCEEEEEHHHCCCCCCCEEEEEECCCCCCEEE
RMVAPFNVSNLLAVIGALVMRGVAVEEALAVVGRLNPPEGRMQLVGGIGEPLIVIDYAHT
EEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHEEECCCCCCEEEEEECCC
PDALAKVLEAVRGTVRSRGGRLVCVFGCGGDRDPGKRPLMGEVARQLADRVVVTSDNPRS
HHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHCEEEEECCCCCC
EDPRKIIDAIAAGAGASADCIVDRAEAIRIAVGEAGPDDVVVLAGKGHEPYQEIHGQRLP
CCHHHHHHHHHCCCCCCCHHHHHHCCEEEEEECCCCCCCEEEEECCCCCCHHHHCCCCCC
FSDVEQAKAALAVWNDRRTTA
CHHHHHHHHHHHEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14500782 [H]