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The map label for this gene is murF [H]

Identifier: 119897171

GI number: 119897171

Start: 940863

End: 942299

Strand: Direct

Name: murF [H]

Synonym: azo0880

Alternate gene names: 119897171

Gene position: 940863-942299 (Clockwise)

Preceding gene: 119897170

Following gene: 119897172

Centisome position: 21.5

GC content: 70.08

Gene sequence:

>1437_bases
GTGGAACGACAGGAGAACGACGGCATGATGAGCCTGCTCGACGCCAGCCGCGCGCTGTCCGCACATCACGCGGTGGCGCA
AGGCGCGGTGCGCTTTTCCAGTGTCGGCACCGACAGCCGCGGCATCATCCCCGGGCAGCTCTTCGTTGCCCTGCGCGGCG
AGCGCTTCGACGGTCACGAATTCGTCGCCGCCGCGCTCAAGGCGGGCGCCGCTGCGGCCATGGTCGACGCGCGTGGCTTT
ACCGAATTCGGCGACGCCAGCCTGCCGCTGCTGGTGGTCGACGACACCCGCCTCGCGCTCGGCACCCTCGCCGCGACGTG
GCGGGCCCGCTTTGCGATTCCGGTCATCGGCGTCACCGGCAGCAACGGCAAGACGACGGTCAAGGAAATGTGCGCCGAGA
TCCTGCGCGCTCAGGCGCGGCGTGATGGCGCCGGGGACGAGGCGGTGCTCGCCACGCGCGGCAACCTCAACAACGACATC
GGTCTGCCGCTGACGCTGCTCGAACTGCGCGACCACCACCGCGCGGCGGTCATCGAGATGGGCATGAACCATCCGGGCGA
GATCGCCTACCTCACCGGGCTTGCACGGCCGACGGTGGCCATCGTCAACAACGCGCAGCGCGCGCACCTGCAGGGCCTGG
GCACGCTGGGGGAAGTGGCGCAGGAGAAGGGCGCGATCTACCAGGGCCTGGGTGCCGCCGGCGTCGCGGTCGTCAACGCC
GACGACCCGCACGCGGGCTACTGGCGCGACCTCAATGCCGGGCGCCCGATTGTGAGCTTTGGTATCGACCAGCCGGCCGA
CGTTGCCGGGGAATGTACCCTGCACGGCCTTGGCTCCCAGCTCCACATCGCCGCGCCCCAGGGACGGGGCGAGGTTGAAC
TGCAGGTGCCGGGGCTGCACAACGCAAGCAACGCGCTCGGCGCCGCCGCCGCCTGTCTTGCCGCGGGCGTGGTGTTCGAA
GCCGCGCTCGAAGGCCTGGCGCTTTACGAAGGCACCCGCGGCCGCCTGCAGCGCAGGTCCGGGCCGCAGGGCGCGGTCAT
CCTCGATGATTCCTACAACGCCAACCCGGATTCGGTGCGCGCCGGCATCGACGTGCTCGCGAGCACGCCGGGTCACACCT
TCCTGGTGCTGGGCGACATGGGCGAAGTGGGGCAGACCAGCGCCCAGGTGCACGACGAAATCGGGGGCTACGCCAAGAGC
AAGGGCATCGACGGCCTGTACGCGCTGGGCGAGGCGAGCGCGATCGCGGTGCGCAATTTCGGCGAAGGCGCGCATCACTT
CGACAGCGTGGAGGCGCTGGTGGCGGCGCTCGCACCCCGGCTCGACGCCGACGCCGTGGTGCTGGTGAAGGGCTCGCGCT
TCATGAAGATGGAGCGGGTGGCGGACGCGCTTGCGGCACTGCACAATGGCGCCCCCCAAAAGGACACGAACTCGTAA

Upstream 100 bases:

>100_bases
TCGTCGTGCTGGCCGGCAAGGGCCACGAACCATATCAGGAGATCCACGGTCAGCGTCTGCCGTTCTCCGACGTCGAGCAG
GCGAAGGCGGCGCTGGCAGT

Downstream 100 bases:

>100_bases
CATGCTGCTCGAACTCGCCCTCTGGCTCGGCCAGGACATCCGCGGTTTCAACGTCTTCGGCTACATCACGCTGCGGACGG
TGCTGGCCGCGCTCACCGCG

Product: UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate-D-alanyl-D-alanine ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MERQENDGMMSLLDASRALSAHHAVAQGAVRFSSVGTDSRGIIPGQLFVALRGERFDGHEFVAAALKAGAAAAMVDARGF
TEFGDASLPLLVVDDTRLALGTLAATWRARFAIPVIGVTGSNGKTTVKEMCAEILRAQARRDGAGDEAVLATRGNLNNDI
GLPLTLLELRDHHRAAVIEMGMNHPGEIAYLTGLARPTVAIVNNAQRAHLQGLGTLGEVAQEKGAIYQGLGAAGVAVVNA
DDPHAGYWRDLNAGRPIVSFGIDQPADVAGECTLHGLGSQLHIAAPQGRGEVELQVPGLHNASNALGAAAACLAAGVVFE
AALEGLALYEGTRGRLQRRSGPQGAVILDDSYNANPDSVRAGIDVLASTPGHTFLVLGDMGEVGQTSAQVHDEIGGYAKS
KGIDGLYALGEASAIAVRNFGEGAHHFDSVEALVAALAPRLDADAVVLVKGSRFMKMERVADALAALHNGAPQKDTNS

Sequences:

>Translated_478_residues
MERQENDGMMSLLDASRALSAHHAVAQGAVRFSSVGTDSRGIIPGQLFVALRGERFDGHEFVAAALKAGAAAAMVDARGF
TEFGDASLPLLVVDDTRLALGTLAATWRARFAIPVIGVTGSNGKTTVKEMCAEILRAQARRDGAGDEAVLATRGNLNNDI
GLPLTLLELRDHHRAAVIEMGMNHPGEIAYLTGLARPTVAIVNNAQRAHLQGLGTLGEVAQEKGAIYQGLGAAGVAVVNA
DDPHAGYWRDLNAGRPIVSFGIDQPADVAGECTLHGLGSQLHIAAPQGRGEVELQVPGLHNASNALGAAAACLAAGVVFE
AALEGLALYEGTRGRLQRRSGPQGAVILDDSYNANPDSVRAGIDVLASTPGHTFLVLGDMGEVGQTSAQVHDEIGGYAKS
KGIDGLYALGEASAIAVRNFGEGAHHFDSVEALVAALAPRLDADAVVLVKGSRFMKMERVADALAALHNGAPQKDTNS
>Mature_478_residues
MERQENDGMMSLLDASRALSAHHAVAQGAVRFSSVGTDSRGIIPGQLFVALRGERFDGHEFVAAALKAGAAAAMVDARGF
TEFGDASLPLLVVDDTRLALGTLAATWRARFAIPVIGVTGSNGKTTVKEMCAEILRAQARRDGAGDEAVLATRGNLNNDI
GLPLTLLELRDHHRAAVIEMGMNHPGEIAYLTGLARPTVAIVNNAQRAHLQGLGTLGEVAQEKGAIYQGLGAAGVAVVNA
DDPHAGYWRDLNAGRPIVSFGIDQPADVAGECTLHGLGSQLHIAAPQGRGEVELQVPGLHNASNALGAAAACLAAGVVFE
AALEGLALYEGTRGRLQRRSGPQGAVILDDSYNANPDSVRAGIDVLASTPGHTFLVLGDMGEVGQTSAQVHDEIGGYAKS
KGIDGLYALGEASAIAVRNFGEGAHHFDSVEALVAALAPRLDADAVVLVKGSRFMKMERVADALAALHNGAPQKDTNS

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=443, Percent_Identity=41.9864559819413, Blast_Score=271, Evalue=6e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 49545; Mature: 49545

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERQENDGMMSLLDASRALSAHHAVAQGAVRFSSVGTDSRGIIPGQLFVALRGERFDGHE
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCEEEECCCCCCCCCCCCEEEEEEECCCCCCHH
FVAAALKAGAAAAMVDARGFTEFGDASLPLLVVDDTRLALGTLAATWRARFAIPVIGVTG
HHHHHHHCCCCEEEEECCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHEEEEEEEEEEC
SNGKTTVKEMCAEILRAQARRDGAGDEAVLATRGNLNNDIGLPLTLLELRDHHRAAVIEM
CCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCEEEEEHHCCCCEEEEEE
GMNHPGEIAYLTGLARPTVAIVNNAQRAHLQGLGTLGEVAQEKGAIYQGLGAAGVAVVNA
CCCCCCCEEEEECCCCCEEEEECCCHHHHHHHCCHHHHHHHHCCCHHCCCCCCCEEEEEC
DDPHAGYWRDLNAGRPIVSFGIDQPADVAGECTLHGLGSQLHIAAPQGRGEVELQVPGLH
CCCCCCCCCCCCCCCCEEEECCCCCCCCCCCEEEEECCCEEEEECCCCCCEEEEECCCCC
NASNALGAAAACLAAGVVFEAALEGLALYEGTRGRLQRRSGPQGAVILDDSYNANPDSVR
CCHHHHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHCCCCCCEEEECCCCCCCHHHHH
AGIDVLASTPGHTFLVLGDMGEVGQTSAQVHDEIGGYAKSKGIDGLYALGEASAIAVRNF
HHHHHEECCCCCEEEEEECCCCCCCHHHHHHHHHCCHHHHCCCCCEEECCCCCEEEEECC
GEGAHHFDSVEALVAALAPRLDADAVVLVKGSRFMKMERVADALAALHNGAPQKDTNS
CCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MERQENDGMMSLLDASRALSAHHAVAQGAVRFSSVGTDSRGIIPGQLFVALRGERFDGHE
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCEEEECCCCCCCCCCCCEEEEEEECCCCCCHH
FVAAALKAGAAAAMVDARGFTEFGDASLPLLVVDDTRLALGTLAATWRARFAIPVIGVTG
HHHHHHHCCCCEEEEECCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHEEEEEEEEEEC
SNGKTTVKEMCAEILRAQARRDGAGDEAVLATRGNLNNDIGLPLTLLELRDHHRAAVIEM
CCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCEEEEEHHCCCCEEEEEE
GMNHPGEIAYLTGLARPTVAIVNNAQRAHLQGLGTLGEVAQEKGAIYQGLGAAGVAVVNA
CCCCCCCEEEEECCCCCEEEEECCCHHHHHHHCCHHHHHHHHCCCHHCCCCCCCEEEEEC
DDPHAGYWRDLNAGRPIVSFGIDQPADVAGECTLHGLGSQLHIAAPQGRGEVELQVPGLH
CCCCCCCCCCCCCCCCEEEECCCCCCCCCCCEEEEECCCEEEEECCCCCCEEEEECCCCC
NASNALGAAAACLAAGVVFEAALEGLALYEGTRGRLQRRSGPQGAVILDDSYNANPDSVR
CCHHHHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHCCCCCCEEEECCCCCCCHHHHH
AGIDVLASTPGHTFLVLGDMGEVGQTSAQVHDEIGGYAKSKGIDGLYALGEASAIAVRNF
HHHHHEECCCCCEEEEEECCCCCCCHHHHHHHHHCCHHHHCCCCCEEECCCCCEEEEECC
GEGAHHFDSVEALVAALAPRLDADAVVLVKGSRFMKMERVADALAALHNGAPQKDTNS
CCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]