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The map label for this gene is wbnF [C]

Identifier: 119897143

GI number: 119897143

Start: 916809

End: 917816

Strand: Direct

Name: wbnF [C]

Synonym: azo0852

Alternate gene names: 119897143

Gene position: 916809-917816 (Clockwise)

Preceding gene: 119897141

Following gene: 119897144

Centisome position: 20.95

GC content: 64.78

Gene sequence:

>1008_bases
ATGAAGATACTCGTCACCGGCGCCGCCGGCTTCATCGGCATGCACACCTCCGAACGTCTGCTCGCCCGCGGGGACGAAGT
CGTCGGACTGGACAACCTCAACGACTACTACGATCCGCGCCTGAAGGAAGACCGTCTGGCGCGGCTGACGCCCAACGACG
GTTTCCGTTTCGTCAGGATGGACGTTGCCGACCGTGCCGGCATGGAGGCGCTGTTCGCGGCCGAGAAATTCGACCGCGTC
ATCCATCTCGCCGCGCAGGCGGGGGTGCGCTATTCGCTGCAGAACCCGCACGCCTACATCGACAGCAACCTGGTGGGCTT
CACCAACATCCTCGAAGGCTGCCGCCACAGCAAGGTCCAGCACCTGGTGTATGCGAGCAGTTCCAGCGTCTATGGCGGCA
ACACGCGCATGCCGTTCTCCGAGCACGACAGCGTGGATCACCCGGTCAGCCTGTACGCCGCCACCAAGAAGGCGAACGAG
CTGATGGCCCACACCTACAGCCATCTGTACGGCCTGCCGACCACCGGGCTGCGCTTCTTCACGGTGTACGGCCCCTGGGG
CCGGCCCGACATGGCGCTGTTCCTGTTCACCAAGGCCATCCTCGAAGGGCGCGCGATCGACGTGTTCAACCACGGCCGCA
TGAAGCGCGACTTCACCTACATCGACGACATCGTCGAAGGTGTGCTGCGCACGCTGGACCGCGTCGCCGAGCCCGATCCG
GCCTTCGATTCCGATCATCCGGACCCCGGCCGTGGCAAGGCGCCCTATCGTGTGTTCAACATCGGCAACAACAACCCGGT
CGAGCTGATGGCCTTCATCGAGGCGATCGAGGGTGCGCTCGGGCGGACCGCCGAGAAGAACTTCCTGCCGCTGCAGGATG
GTGACGTGCCGGCCACCTACGCCAATACCGACGAGCTCAACGCGTGGACCGGGTTTGCGCCGGCAACCAGTGTCAGCGAC
GGCGTCGGCCGCTTCGTCGCCTGGTACCGCGCTTACTACGGCCTCTAA

Upstream 100 bases:

>100_bases
CGATTCGGCTCGCGGAGTATAGCCCGCGGTCGTGCTTCTTCATGGTTGACAGTCGTTTGCCATGCGATAATCGGCGCGAC
CCATTCTTGCGGCTTATCCC

Downstream 100 bases:

>100_bases
GTCCGCGCTGAACGCCCGGCGATGGCGACCTACGCGATCGGCGACATCCAGGGCTGTTACGAGCCGTTGCGGCGGCTGCT
CGATCTGATCCGCTTCGACC

Product: putative nucleotide sugar epimerase

Products: UDPglucoseal [C]

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 335; Mature: 335

Protein sequence:

>335_residues
MKILVTGAAGFIGMHTSERLLARGDEVVGLDNLNDYYDPRLKEDRLARLTPNDGFRFVRMDVADRAGMEALFAAEKFDRV
IHLAAQAGVRYSLQNPHAYIDSNLVGFTNILEGCRHSKVQHLVYASSSSVYGGNTRMPFSEHDSVDHPVSLYAATKKANE
LMAHTYSHLYGLPTTGLRFFTVYGPWGRPDMALFLFTKAILEGRAIDVFNHGRMKRDFTYIDDIVEGVLRTLDRVAEPDP
AFDSDHPDPGRGKAPYRVFNIGNNNPVELMAFIEAIEGALGRTAEKNFLPLQDGDVPATYANTDELNAWTGFAPATSVSD
GVGRFVAWYRAYYGL

Sequences:

>Translated_335_residues
MKILVTGAAGFIGMHTSERLLARGDEVVGLDNLNDYYDPRLKEDRLARLTPNDGFRFVRMDVADRAGMEALFAAEKFDRV
IHLAAQAGVRYSLQNPHAYIDSNLVGFTNILEGCRHSKVQHLVYASSSSVYGGNTRMPFSEHDSVDHPVSLYAATKKANE
LMAHTYSHLYGLPTTGLRFFTVYGPWGRPDMALFLFTKAILEGRAIDVFNHGRMKRDFTYIDDIVEGVLRTLDRVAEPDP
AFDSDHPDPGRGKAPYRVFNIGNNNPVELMAFIEAIEGALGRTAEKNFLPLQDGDVPATYANTDELNAWTGFAPATSVSD
GVGRFVAWYRAYYGL
>Mature_335_residues
MKILVTGAAGFIGMHTSERLLARGDEVVGLDNLNDYYDPRLKEDRLARLTPNDGFRFVRMDVADRAGMEALFAAEKFDRV
IHLAAQAGVRYSLQNPHAYIDSNLVGFTNILEGCRHSKVQHLVYASSSSVYGGNTRMPFSEHDSVDHPVSLYAATKKANE
LMAHTYSHLYGLPTTGLRFFTVYGPWGRPDMALFLFTKAILEGRAIDVFNHGRMKRDFTYIDDIVEGVLRTLDRVAEPDP
AFDSDHPDPGRGKAPYRVFNIGNNNPVELMAFIEAIEGALGRTAEKNFLPLQDGDVPATYANTDELNAWTGFAPATSVSD
GVGRFVAWYRAYYGL

Specific function: Galactose metabolism; third step. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=341, Percent_Identity=26.3929618768328, Blast_Score=120, Evalue=1e-27,
Organism=Homo sapiens, GI56237023, Length=334, Percent_Identity=27.8443113772455, Blast_Score=106, Evalue=3e-23,
Organism=Homo sapiens, GI56118217, Length=334, Percent_Identity=27.8443113772455, Blast_Score=106, Evalue=3e-23,
Organism=Homo sapiens, GI189083684, Length=334, Percent_Identity=27.8443113772455, Blast_Score=106, Evalue=3e-23,
Organism=Homo sapiens, GI42516563, Length=338, Percent_Identity=24.5562130177515, Blast_Score=99, Evalue=7e-21,
Organism=Homo sapiens, GI193211614, Length=298, Percent_Identity=25.1677852348993, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI8393516, Length=298, Percent_Identity=25.1677852348993, Blast_Score=73, Evalue=3e-13,
Organism=Escherichia coli, GI1786974, Length=325, Percent_Identity=25.5384615384615, Blast_Score=108, Evalue=7e-25,
Organism=Escherichia coli, GI1788353, Length=359, Percent_Identity=25.9052924791086, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI48994969, Length=355, Percent_Identity=25.6338028169014, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1788589, Length=250, Percent_Identity=27.6, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1790049, Length=349, Percent_Identity=24.0687679083095, Blast_Score=62, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI71982035, Length=352, Percent_Identity=26.1363636363636, Blast_Score=113, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI71982038, Length=354, Percent_Identity=25.9887005649718, Blast_Score=112, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI17539532, Length=340, Percent_Identity=26.7647058823529, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17568069, Length=235, Percent_Identity=29.3617021276596, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI115532424, Length=345, Percent_Identity=24.3478260869565, Blast_Score=66, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6319493, Length=137, Percent_Identity=40.1459854014599, Blast_Score=87, Evalue=3e-18,
Organism=Drosophila melanogaster, GI19923002, Length=322, Percent_Identity=27.0186335403727, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI21356223, Length=340, Percent_Identity=24.1176470588235, Blast_Score=89, Evalue=6e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040
- InterPro:   IPR008089 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 5.1.3.2 [C]

Molecular weight: Translated: 37254; Mature: 37254

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILVTGAAGFIGMHTSERLLARGDEVVGLDNLNDYYDPRLKEDRLARLTPNDGFRFVRM
CEEEEEECCCEEECCCHHHHHHCCCCEEECCCCCHHCCCCCCCCCCEECCCCCCEEEEEE
DVADRAGMEALFAAEKFDRVIHLAAQAGVRYSLQNPHAYIDSNLVGFTNILEGCRHSKVQ
ECHHHHCHHHHHHHHHHHHHHHHHHHCCCEEECCCCCEEECCCCCHHHHHHHHHHHHHHH
HLVYASSSSVYGGNTRMPFSEHDSVDHPVSLYAATKKANELMAHTYSHLYGLPTTGLRFF
EEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCEEEE
TVYGPWGRPDMALFLFTKAILEGRAIDVFNHGRMKRDFTYIDDIVEGVLRTLDRVAEPDP
EEECCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
AFDSDHPDPGRGKAPYRVFNIGNNNPVELMAFIEAIEGALGRTAEKNFLPLQDGDVPATY
CCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEE
ANTDELNAWTGFAPATSVSDGVGRFVAWYRAYYGL
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKILVTGAAGFIGMHTSERLLARGDEVVGLDNLNDYYDPRLKEDRLARLTPNDGFRFVRM
CEEEEEECCCEEECCCHHHHHHCCCCEEECCCCCHHCCCCCCCCCCEECCCCCCEEEEEE
DVADRAGMEALFAAEKFDRVIHLAAQAGVRYSLQNPHAYIDSNLVGFTNILEGCRHSKVQ
ECHHHHCHHHHHHHHHHHHHHHHHHHCCCEEECCCCCEEECCCCCHHHHHHHHHHHHHHH
HLVYASSSSVYGGNTRMPFSEHDSVDHPVSLYAATKKANELMAHTYSHLYGLPTTGLRFF
EEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCEEEE
TVYGPWGRPDMALFLFTKAILEGRAIDVFNHGRMKRDFTYIDDIVEGVLRTLDRVAEPDP
EEECCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
AFDSDHPDPGRGKAPYRVFNIGNNNPVELMAFIEAIEGALGRTAEKNFLPLQDGDVPATY
CCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEE
ANTDELNAWTGFAPATSVSDGVGRFVAWYRAYYGL
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 57600 [C]

Specific activity: 233.3

Km value (mM): 0.256 {UDPgalactose}} 0.225 {UDPgalactose}} 0.2 {UDPgalactose}} 0.18 {UDPgalactose}} 0.16 {UDPgalactose}} 0.14 {UDPgalactose}} 0.048 {UDPgalactose}} 0.026 {UDPgalactose}} [C]

Substrates: UDPglucose [C]

Specific reaction: UDPglucose <==> UDPglucoseal [C]

General reaction: Epimerization (specificity for carbon forming a hexosulose) [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7682279 [H]