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The map label for this gene is tolC [H]

Identifier: 119897141

GI number: 119897141

Start: 914066

End: 915382

Strand: Direct

Name: tolC [H]

Synonym: azo0850

Alternate gene names: 119897141

Gene position: 914066-915382 (Clockwise)

Preceding gene: 119897140

Following gene: 119897143

Centisome position: 20.89

GC content: 69.7

Gene sequence:

>1317_bases
ATGAAGCGATCGCTTGCTGCGAAGCTGTTCGCGGCGGGGTTGGCTGCGGCCTTCGGCGCACCAGCGGCCGCGGCGGACCT
GCTGCAGACCTACCGCGACGCGCTTGCCAACGACGCGCGCTACGCCGCCGCCCGGGCGGAGCGCGACGCCGGCATGGAGC
GGGTGGTGCAGGCGCGCGCCGGGTTGCTGCCCAACCTCACGGCCTCGGCGTCCACCGCCTATAACGACGGCGAGGCGCGC
ACCTCCGCGGTGTCCGTCGATCGGCGCTACAACAGCAACGGCTACGCGGTGCAGCTGACCCAGCCGCTGTTCCGCTGGCA
GAACTGGGTGCAGTACAAGCAGGGCGAACTGCAGACGGCGCTGGCCGAAACCCAGTTCGGCCAGGCGCGGCAGGATCTGA
TCCTGCGCGTCGCGGAAGCCTACTTCAACGTACTCAATGCGCAGGACGCGCTCGACGCCGTCGTGCAATTGCGCACCGCC
GCCGCCGAGCAACTCGAACTCGCGCGTCGCAGCTTCGAAGTCGGAACGGTCACCATCACCGACGTGCATGAGGCGCAGTC
GCGCTACGACCTCGCCTCCGCGCAGGAGATCGCCGCGCGCAACGATCTCGATGTCCAGCGTCAGACGCTGGCGCAGATCA
TCGGCCGCGAACCGGATCCGCTGGCGCCACTGCGCGCCGGCGTCGAACTGCAGCGCCCGCAACCAGACAGCATCGCAGAC
TGGGTGGGCGCGGCGGAGCAGGGCAGTTTCGGCGTCCAGGGGCAGCAGCTCAACCGCGAGATCGCTGCGCGCGAAGTCGA
GCGCGCGCGCGCCGGCCATCTGCCGACGCTGGATCTTGTCGCCTCGCGCGGGCTGAATCAGCGCCCGTCGATCACCACCG
AACGCAGCGAAGCCAGCTCCATCGGGCTGCAGCTCAATGTGCCCTTGTACCAAGGCGGCGCGGTGTCCTCGCGCGAGCGC
GAAACCGCCGCGCTCAAGCTCAAGGCCGACGCGGATCTGGAGGACGCGCGCCGCGGCGCCGCACTGGCCGCCCGCCAGTC
CTACCTCGGCGTCACCAGTGGTCTAGCGCAGATTCGTGCGCTCGAGGCGGCGCGGATATCCTCGGCCTCGGCGCTCGAAG
CGAACCGCCTGGGTTACGAGGTGGGGGTGCGGATCAATATCGACGTGCTCAACGCCCAGACCCAACTGGCCGACACCCAG
CAGCGTCTCGCGCGTGCACGCTATGACACGCTGCTGGCGCAACTGCGGCTGAAGGCGGCGGCCGGTACGCTGGGCGACGA
GGACGTCCAGGCGATCAACGCGCTGCTCGCGCCTTAA

Upstream 100 bases:

>100_bases
GGGGTTCAAGCGGGTGATCAATCTCGCCGGCGGCGTGGCCGAGTGGGCGGCGAAGGTCGATCCTTCGATGCCTCATTACT
GAGCTGCGCGGGAGGCCGGG

Downstream 100 bases:

>100_bases
GGGCGCGCAGCGTCCGCTTGCTGCAGCAGGTCGAGCGTGCGTGCGGTGGCGCCGCGGTGCGCCGCCGCGAAGCCGCGTCC
GGCTTCCGCCAGGGCTGTGC

Product: putative outer membrane efflux protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 438; Mature: 438

Protein sequence:

>438_residues
MKRSLAAKLFAAGLAAAFGAPAAAADLLQTYRDALANDARYAAARAERDAGMERVVQARAGLLPNLTASASTAYNDGEAR
TSAVSVDRRYNSNGYAVQLTQPLFRWQNWVQYKQGELQTALAETQFGQARQDLILRVAEAYFNVLNAQDALDAVVQLRTA
AAEQLELARRSFEVGTVTITDVHEAQSRYDLASAQEIAARNDLDVQRQTLAQIIGREPDPLAPLRAGVELQRPQPDSIAD
WVGAAEQGSFGVQGQQLNREIAAREVERARAGHLPTLDLVASRGLNQRPSITTERSEASSIGLQLNVPLYQGGAVSSRER
ETAALKLKADADLEDARRGAALAARQSYLGVTSGLAQIRALEAARISSASALEANRLGYEVGVRINIDVLNAQTQLADTQ
QRLARARYDTLLAQLRLKAAAGTLGDEDVQAINALLAP

Sequences:

>Translated_438_residues
MKRSLAAKLFAAGLAAAFGAPAAAADLLQTYRDALANDARYAAARAERDAGMERVVQARAGLLPNLTASASTAYNDGEAR
TSAVSVDRRYNSNGYAVQLTQPLFRWQNWVQYKQGELQTALAETQFGQARQDLILRVAEAYFNVLNAQDALDAVVQLRTA
AAEQLELARRSFEVGTVTITDVHEAQSRYDLASAQEIAARNDLDVQRQTLAQIIGREPDPLAPLRAGVELQRPQPDSIAD
WVGAAEQGSFGVQGQQLNREIAAREVERARAGHLPTLDLVASRGLNQRPSITTERSEASSIGLQLNVPLYQGGAVSSRER
ETAALKLKADADLEDARRGAALAARQSYLGVTSGLAQIRALEAARISSASALEANRLGYEVGVRINIDVLNAQTQLADTQ
QRLARARYDTLLAQLRLKAAAGTLGDEDVQAINALLAP
>Mature_438_residues
MKRSLAAKLFAAGLAAAFGAPAAAADLLQTYRDALANDARYAAARAERDAGMERVVQARAGLLPNLTASASTAYNDGEAR
TSAVSVDRRYNSNGYAVQLTQPLFRWQNWVQYKQGELQTALAETQFGQARQDLILRVAEAYFNVLNAQDALDAVVQLRTA
AAEQLELARRSFEVGTVTITDVHEAQSRYDLASAQEIAARNDLDVQRQTLAQIIGREPDPLAPLRAGVELQRPQPDSIAD
WVGAAEQGSFGVQGQQLNREIAAREVERARAGHLPTLDLVASRGLNQRPSITTERSEASSIGLQLNVPLYQGGAVSSRER
ETAALKLKADADLEDARRGAALAARQSYLGVTSGLAQIRALEAARISSASALEANRLGYEVGVRINIDVLNAQTQLADTQ
QRLARARYDTLLAQLRLKAAAGTLGDEDVQAINALLAP

Specific function: Required for proper expression of outer membrane protein genes such as ompF, nmpC, protein 2, hemolysin, colicin V, or colicin E1. May be specialized for signal sequence independent, extracellular secretion in Gram-negative bacteria [H]

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI87082199, Length=427, Percent_Identity=33.4894613583138, Blast_Score=220, Evalue=1e-58,

Paralogues:

None

Copy number: 120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010130 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 47136; Mature: 47136

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
0.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRSLAAKLFAAGLAAAFGAPAAAADLLQTYRDALANDARYAAARAERDAGMERVVQARA
CCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
GLLPNLTASASTAYNDGEARTSAVSVDRRYNSNGYAVQLTQPLFRWQNWVQYKQGELQTA
CCCCCCCCCCCCCCCCCCHHHHHEEHHHEECCCCCEEEECHHHHHHHHHHHHHHHHHHHH
LAETQFGQARQDLILRVAEAYFNVLNAQDALDAVVQLRTAAAEQLELARRSFEVGTVTIT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
DVHEAQSRYDLASAQEIAARNDLDVQRQTLAQIIGREPDPLAPLRAGVELQRPQPDSIAD
EHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCCCCHHHH
WVGAAEQGSFGVQGQQLNREIAAREVERARAGHLPTLDLVASRGLNQRPSITTERSEASS
HHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCCCHHHH
IGLQLNVPLYQGGAVSSRERETAALKLKADADLEDARRGAALAARQSYLGVTSGLAQIRA
CCEEEECCEECCCCCCCCCCCCEEEEEECCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHH
LEAARISSASALEANRLGYEVGVRINIDVLNAQTQLADTQQRLARARYDTLLAQLRLKAA
HHHHHCCCHHHHHHHHCCEEEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGTLGDEDVQAINALLAP
HCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MKRSLAAKLFAAGLAAAFGAPAAAADLLQTYRDALANDARYAAARAERDAGMERVVQARA
CCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
GLLPNLTASASTAYNDGEARTSAVSVDRRYNSNGYAVQLTQPLFRWQNWVQYKQGELQTA
CCCCCCCCCCCCCCCCCCHHHHHEEHHHEECCCCCEEEECHHHHHHHHHHHHHHHHHHHH
LAETQFGQARQDLILRVAEAYFNVLNAQDALDAVVQLRTAAAEQLELARRSFEVGTVTIT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
DVHEAQSRYDLASAQEIAARNDLDVQRQTLAQIIGREPDPLAPLRAGVELQRPQPDSIAD
EHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCCCCHHHH
WVGAAEQGSFGVQGQQLNREIAAREVERARAGHLPTLDLVASRGLNQRPSITTERSEASS
HHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCCCHHHH
IGLQLNVPLYQGGAVSSRERETAALKLKADADLEDARRGAALAARQSYLGVTSGLAQIRA
CCEEEECCEECCCCCCCCCCCCEEEEEECCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHH
LEAARISSASALEANRLGYEVGVRINIDVLNAQTQLADTQQRLARARYDTLLAQLRLKAA
HHHHHCCCHHHHHHHHCCEEEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGTLGDEDVQAINALLAP
HCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 6312426; 2216730; 9278503; 6303857; 9298646; 9629924; 10879525 [H]