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The map label for this gene is nuoB1

Identifier: 119897120

GI number: 119897120

Start: 887858

End: 888337

Strand: Reverse

Name: nuoB1

Synonym: azo0829

Alternate gene names: 119897120

Gene position: 888337-887858 (Counterclockwise)

Preceding gene: 119897121

Following gene: 119897115

Centisome position: 20.3

GC content: 66.67

Gene sequence:

>480_bases
GTGCAAACCCCCACCCTGCATGGCGACGGCTTCCTGCTGACGCGGCTCGACACGCTGTGCGACATGGTCCGCAGCAACAG
CATGTGGTACCTCACCTTCGGCCTCGCCTGCTGCGCGGTCGAGATGATGCAGGCCGCGGCCTCGCGCTATGACATGGACC
GCTTCGGCATGATCCCGCGCGCCAGCCCGCGTCAGGCCGACCTGATCATCGTCGCCGGCACGCTCACCAACAAGATGGCG
CCGGCGATCCGCAAGATCTACGACCAGATGGCCGAACCGCGCTACGTGATCTCGATGGGTTCGTGCGCCAACGGCGGCGG
CTACTACCACTATTCGTACTCGGTGGCGCGCGGCTGCGACCGCATCCTGCCCGTGGACATCTACATCCCCGGCTGTCCGC
CCACCGCGGAAGCGCTGCTGTACGGGCTGATCCAGCTGCAGAACAAGCTGAAGCGGCCGCCCGCGGTGATCGCCCGGTAA

Upstream 100 bases:

>100_bases
AGTTCCGCGACCTCTTCCTGCCGGACGATGTCGACTCGGTACAAGCCCGCCTGTTCGCCCCTGCCTAGACTGCGCCCCGT
TCCATCTCCGGGAGAGAACG

Downstream 100 bases:

>100_bases
ACGCAGGACGGGGCCCGCAGGCCCCGTCGTCGATGCAGCCGCTTCACGCCGGCACGCCGGCGCGGCGTCAGAACACCTCG
TCGTCGCGCAGATAGCGCCA

Product: NADH-quinone oxidoreductase subunit B

Products: NA

Alternate protein names: NADH dehydrogenase I subunit B 2; NDH-1 subunit B 2

Number of amino acids: Translated: 159; Mature: 159

Protein sequence:

>159_residues
MQTPTLHGDGFLLTRLDTLCDMVRSNSMWYLTFGLACCAVEMMQAAASRYDMDRFGMIPRASPRQADLIIVAGTLTNKMA
PAIRKIYDQMAEPRYVISMGSCANGGGYYHYSYSVARGCDRILPVDIYIPGCPPTAEALLYGLIQLQNKLKRPPAVIAR

Sequences:

>Translated_159_residues
MQTPTLHGDGFLLTRLDTLCDMVRSNSMWYLTFGLACCAVEMMQAAASRYDMDRFGMIPRASPRQADLIIVAGTLTNKMA
PAIRKIYDQMAEPRYVISMGSCANGGGYYHYSYSVARGCDRILPVDIYIPGCPPTAEALLYGLIQLQNKLKRPPAVIAR
>Mature_159_residues
MQTPTLHGDGFLLTRLDTLCDMVRSNSMWYLTFGLACCAVEMMQAAASRYDMDRFGMIPRASPRQADLIIVAGTLTNKMA
PAIRKIYDQMAEPRYVISMGSCANGGGYYHYSYSVARGCDRILPVDIYIPGCPPTAEALLYGLIQLQNKLKRPPAVIAR

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cyto

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family

Homologues:

Organism=Homo sapiens, GI187281616, Length=149, Percent_Identity=73.8255033557047, Blast_Score=242, Evalue=1e-64,
Organism=Escherichia coli, GI1788624, Length=146, Percent_Identity=51.3698630136986, Blast_Score=154, Evalue=3e-39,
Organism=Escherichia coli, GI1789074, Length=111, Percent_Identity=42.3423423423423, Blast_Score=97, Evalue=5e-22,
Organism=Escherichia coli, GI1788834, Length=111, Percent_Identity=39.6396396396396, Blast_Score=93, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI17509685, Length=142, Percent_Identity=73.2394366197183, Blast_Score=231, Evalue=9e-62,
Organism=Drosophila melanogaster, GI18859983, Length=142, Percent_Identity=77.4647887323944, Blast_Score=241, Evalue=2e-64,
Organism=Drosophila melanogaster, GI24642371, Length=142, Percent_Identity=77.4647887323944, Blast_Score=241, Evalue=2e-64,
Organism=Drosophila melanogaster, GI24651058, Length=138, Percent_Identity=78.9855072463768, Blast_Score=235, Evalue=8e-63,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NUOB2_AZOSB (A1K3P1)

Other databases:

- EMBL:   AM406670
- RefSeq:   YP_932333.1
- ProteinModelPortal:   A1K3P1
- SMR:   A1K3P1
- STRING:   A1K3P1
- GeneID:   4606594
- GenomeReviews:   AM406670_GR
- KEGG:   azo:azo0829
- eggNOG:   COG0377
- HOGENOM:   HBG553221
- OMA:   RRANDRF
- BioCyc:   ASP62928:AZO0829-MONOMER
- GO:   GO:0006810
- HAMAP:   MF_01356
- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406
- PANTHER:   PTHR11995:SF2
- PANTHER:   PTHR11995
- TIGRFAMs:   TIGR01957

Pfam domain/function: PF01058 Oxidored_q6

EC number: =1.6.99.5

Molecular weight: Translated: 17621; Mature: 17621

Theoretical pI: Translated: 8.57; Mature: 8.57

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.8 %Cys     (Translated Protein)
6.3 %Met     (Translated Protein)
10.1 %Cys+Met (Translated Protein)
3.8 %Cys     (Mature Protein)
6.3 %Met     (Mature Protein)
10.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTPTLHGDGFLLTRLDTLCDMVRSNSMWYLTFGLACCAVEMMQAAASRYDMDRFGMIPR
CCCCCCCCCCHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHCCHHHHCCCCC
ASPRQADLIIVAGTLTNKMAPAIRKIYDQMAEPRYVISMGSCANGGGYYHYSYSVARGCD
CCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEEEHHHCCCC
RILPVDIYIPGCPPTAEALLYGLIQLQNKLKRPPAVIAR
CEEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MQTPTLHGDGFLLTRLDTLCDMVRSNSMWYLTFGLACCAVEMMQAAASRYDMDRFGMIPR
CCCCCCCCCCHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHCCHHHHCCCCC
ASPRQADLIIVAGTLTNKMAPAIRKIYDQMAEPRYVISMGSCANGGGYYHYSYSVARGCD
CCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEEEHHHCCCC
RILPVDIYIPGCPPTAEALLYGLIQLQNKLKRPPAVIAR
CEEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA