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The map label for this gene is gspF1 [H]

Identifier: 119897093

GI number: 119897093

Start: 859072

End: 860277

Strand: Reverse

Name: gspF1 [H]

Synonym: azo0802

Alternate gene names: 119897093

Gene position: 860277-859072 (Counterclockwise)

Preceding gene: 119897094

Following gene: 119897092

Centisome position: 19.66

GC content: 72.31

Gene sequence:

>1206_bases
ATGAGCAGACTGCAGGCCCATGCCTATCGCGCGGTCAACGGCGACGGCCGCCGGGTGCGCGGTCGGGCCCACGCGCTGCA
CCTCAACGATCTCGAAGCACGGCTCGCCACCCGCGGGCTTACCCTCATCCACGGCGAAGTGGCGCGCCGCAGCCTTCGGC
CCCAGCCTCGCCTGCCCGCGCGCGAGCTGGGCGCGTTCTGCGCCCAGCTCGCCCATCTGCTGGAGGCCGGCATCCCGCTG
GTGGAGGCCCTGCGCGACCTCGGCGAGGGCAACGCGACGCCCGCCGCCCGCCCCTGGGACGACATCGCGACCGCGCTCGA
GGGCGGCGACACCCTGTCCGACGCCTGCGCCGCGCGCGCCGATCTGTTCGACGAGGTCTTCGTCAGCCTGCTGCGCGCCG
GCGAGCAGGCCGGGCGCCTCCCCGCAGTCCTGCACCATCTGGCGGCGACGCTGGAACGCAGTGCGCAGGTGGCCGCCCAT
CTACGCCGGATGTCTATCTATCCCGCCTTCGTACTTGCGGTTCTTGCGGTCGCGGCCGGAACAGCCCTGGTCTTCGTGGT
GCCGCAACTGAGCTCCCTGTTCCGCAGCCTGGGCGAAACGCTGCCGTGGCAGACCCGCGCGCTGCTCAGCCTGTCGGACC
TCGTGCTCAGGCACGGTGCCTGGGCCACGGTCGTGGCGATCCTTTGCCTCGCTGGCTTGCGCCTCGCGCTCCTGCGCAGC
ATCGCATTCCGCCTTCAGGTCCACGCCGCGCTGCTGCGTCTGCCGGTGGTCGGCGGGCTGTGGCACACCGCGCTGCTCGC
CCGCGTCTGCCAGGTCCTCGCGGTGTCCTACGAGGCCGGCGTGCCGATCGTCGCCGCAGTCACCGCCGCCGCCGGCACCG
CAGGCAACCTTGTGGTCCGCACCGGCCTGGAGCAGGTCGCCGACGACATTGCGGCCGGGGCAGATCTGAGCGGAGCGCTT
GCCTCAACCCGCGTGTTCCCGCCGCGGGTCACACGGATGCTGCGGGCCGGCGAGCACAGCGGTACGCTCGACCGCGCCCT
GCGGCACGCCGGCGCCTTCTACGAGCGCGAACTGCAGGAAGGCGTCGCGCGCCTGCAAGCCGCGGTCGAGCCGGCGCTCA
CTGTGCTGCTCGGCCTGCTGATGCTGTGGATCATGAGCGCGATCCTCGGCCCCATCTACGACGTCCTCGGAAAGCTGCCG
CTGTAG

Upstream 100 bases:

>100_bases
GCCATGTCGGGCTTGCCGCTGCGGGGCTCGCCCGGGTGCGGGCCGGAGACACCACGCTGGAAGAACTGCTGCGCGTCGTC
GACGTCCGTGGAGCCCGGCG

Downstream 100 bases:

>100_bases
GCCGCCATGGGACGAGACTGCCTGCTCCTGATTTCCGCGGCCGGCGCAAGGGCATGGACGCAGCAGCGCGGCGGCCTGGC
GCCCGGACCGCACTTTGCCG

Product: general secretion pathway protein F

Products: NA

Alternate protein names: Type IV-A pilus assembly protein pilC [H]

Number of amino acids: Translated: 401; Mature: 400

Protein sequence:

>401_residues
MSRLQAHAYRAVNGDGRRVRGRAHALHLNDLEARLATRGLTLIHGEVARRSLRPQPRLPARELGAFCAQLAHLLEAGIPL
VEALRDLGEGNATPAARPWDDIATALEGGDTLSDACAARADLFDEVFVSLLRAGEQAGRLPAVLHHLAATLERSAQVAAH
LRRMSIYPAFVLAVLAVAAGTALVFVVPQLSSLFRSLGETLPWQTRALLSLSDLVLRHGAWATVVAILCLAGLRLALLRS
IAFRLQVHAALLRLPVVGGLWHTALLARVCQVLAVSYEAGVPIVAAVTAAAGTAGNLVVRTGLEQVADDIAAGADLSGAL
ASTRVFPPRVTRMLRAGEHSGTLDRALRHAGAFYERELQEGVARLQAAVEPALTVLLGLLMLWIMSAILGPIYDVLGKLP
L

Sequences:

>Translated_401_residues
MSRLQAHAYRAVNGDGRRVRGRAHALHLNDLEARLATRGLTLIHGEVARRSLRPQPRLPARELGAFCAQLAHLLEAGIPL
VEALRDLGEGNATPAARPWDDIATALEGGDTLSDACAARADLFDEVFVSLLRAGEQAGRLPAVLHHLAATLERSAQVAAH
LRRMSIYPAFVLAVLAVAAGTALVFVVPQLSSLFRSLGETLPWQTRALLSLSDLVLRHGAWATVVAILCLAGLRLALLRS
IAFRLQVHAALLRLPVVGGLWHTALLARVCQVLAVSYEAGVPIVAAVTAAAGTAGNLVVRTGLEQVADDIAAGADLSGAL
ASTRVFPPRVTRMLRAGEHSGTLDRALRHAGAFYERELQEGVARLQAAVEPALTVLLGLLMLWIMSAILGPIYDVLGKLP
L
>Mature_400_residues
SRLQAHAYRAVNGDGRRVRGRAHALHLNDLEARLATRGLTLIHGEVARRSLRPQPRLPARELGAFCAQLAHLLEAGIPLV
EALRDLGEGNATPAARPWDDIATALEGGDTLSDACAARADLFDEVFVSLLRAGEQAGRLPAVLHHLAATLERSAQVAAHL
RRMSIYPAFVLAVLAVAAGTALVFVVPQLSSLFRSLGETLPWQTRALLSLSDLVLRHGAWATVVAILCLAGLRLALLRSI
AFRLQVHAALLRLPVVGGLWHTALLARVCQVLAVSYEAGVPIVAAVTAAAGTAGNLVVRTGLEQVADDIAAGADLSGALA
STRVFPPRVTRMLRAGEHSGTLDRALRHAGAFYERELQEGVARLQAAVEPALTVLLGLLMLWIMSAILGPIYDVLGKLPL

Specific function: Involved in the translocation of the type IV pilin [H]

COG id: COG1459

COG function: function code NU; Type II secretory pathway, component PulF

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP F family [H]

Homologues:

Organism=Escherichia coli, GI1789724, Length=396, Percent_Identity=30.050505050505, Blast_Score=158, Evalue=7e-40,
Organism=Escherichia coli, GI1786295, Length=298, Percent_Identity=28.1879194630872, Blast_Score=105, Evalue=5e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003004
- InterPro:   IPR018076
- InterPro:   IPR001992 [H]

Pfam domain/function: PF00482 GSPII_F [H]

EC number: NA

Molecular weight: Translated: 42621; Mature: 42490

Theoretical pI: Translated: 9.63; Mature: 9.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRLQAHAYRAVNGDGRRVRGRAHALHLNDLEARLATRGLTLIHGEVARRSLRPQPRLPA
CCCHHHHHHHHCCCCCCEECCHHHEEEHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCH
RELGAFCAQLAHLLEAGIPLVEALRDLGEGNATPAARPWDDIATALEGGDTLSDACAARA
HHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHH
DLFDEVFVSLLRAGEQAGRLPAVLHHLAATLERSAQVAAHLRRMSIYPAFVLAVLAVAAG
HHHHHHHHHHHHCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TALVFVVPQLSSLFRSLGETLPWQTRALLSLSDLVLRHGAWATVVAILCLAGLRLALLRS
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
IAFRLQVHAALLRLPVVGGLWHTALLARVCQVLAVSYEAGVPIVAAVTAAAGTAGNLVVR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHH
TGLEQVADDIAAGADLSGALASTRVFPPRVTRMLRAGEHSGTLDRALRHAGAFYERELQE
HHHHHHHHHHHCCCCCHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
GVARLQAAVEPALTVLLGLLMLWIMSAILGPIYDVLGKLPL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SRLQAHAYRAVNGDGRRVRGRAHALHLNDLEARLATRGLTLIHGEVARRSLRPQPRLPA
CCHHHHHHHHCCCCCCEECCHHHEEEHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCH
RELGAFCAQLAHLLEAGIPLVEALRDLGEGNATPAARPWDDIATALEGGDTLSDACAARA
HHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHH
DLFDEVFVSLLRAGEQAGRLPAVLHHLAATLERSAQVAAHLRRMSIYPAFVLAVLAVAAG
HHHHHHHHHHHHCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TALVFVVPQLSSLFRSLGETLPWQTRALLSLSDLVLRHGAWATVVAILCLAGLRLALLRS
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
IAFRLQVHAALLRLPVVGGLWHTALLARVCQVLAVSYEAGVPIVAAVTAAAGTAGNLVVR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHH
TGLEQVADDIAAGADLSGALASTRVFPPRVTRMLRAGEHSGTLDRALRHAGAFYERELQE
HHHHHHHHHHHCCCCCHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
GVARLQAAVEPALTVLLGLLMLWIMSAILGPIYDVLGKLPL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10024587; 10952301 [H]