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The map label for this gene is gspE1 [H]

Identifier: 119897094

GI number: 119897094

Start: 860274

End: 861974

Strand: Reverse

Name: gspE1 [H]

Synonym: azo0803

Alternate gene names: 119897094

Gene position: 861974-860274 (Counterclockwise)

Preceding gene: 119897095

Following gene: 119897093

Centisome position: 19.7

GC content: 70.61

Gene sequence:

>1701_bases
GTGGCGCCCCTCCCCGATCACGACGCCCTCGCCACGCTGTTGCTCGCGGCCGGCGTCATCGGACAGGACCAGCTGCGCAT
TGCCCGAATGGAGCAGCAGCGCGCCCCGCGGCCGCTGGCAGATCTGCTGCTCGGGCTGGGATTCCTGTCCGAGTCGGCAC
TGCGCGGCGTGATCTCCGAAGCGAGCGGACGGCGGCCGGTCGACCTCGCCCGCAGCCTGCCGGATGACGCGGCGCTCGCC
CTGGTGCCGGTCGAATTCGCGCGGCGGAACCAGTTACTGCCCCTGAGCCTGGATACGGACCAGGCCGTGCTCACCATCGC
CACCGCAGCAACCGACGACCGCGCCGTCATCGACAAGCTGCTCGCGCTGTGTCCGCAGTTGCGCCGGGTGGAATGCCTGC
TGGCAGCAGGCCCCGAACTCGGCCGCGCGATCGAGCGCCACTACGGCCAGGCGCAGTCGCTCGAGAGCATCCTGCACGAA
CTCGAAACCGGTGCCCCGCCCCCACCCCAGCGGCCCGGCGGCGAGGGACACCCCCAGGCCCTGGTCCGGCTGGTGGATGC
GCTGCTGCTCGACGCGGTGAAGCGCGACGCATCCGACATCCACTTCGAACCCGAGGCCCACCATCTGCGCATCCGCTGCC
GCATCGACGGTGTGTTGCGGCAGACCCGCGCGCTTCACGCTACCTACTGGCCGGCAATGGCGGTGCGGATCAAGGTCCTT
GCCGGCCTCAACATTGCCGAGACCCGTGTCCCGCAGGACGGCCGCTTCTCGTTCGACGTCGCCGGCCATGCCATCGACTT
CCGCGTTTCGTGCATGCCGACGCTGCACGGCGAAAACGTCGTGCTGCGCCTGCTCGACCGCAGCAAGGGCGTGGTCCCGC
TCTCCGACCTCGGCTTGGGGGCCGGAGACCTGGCCGCGCTCGAAGCGATGATCGCCCGCCCCGAGGGCCTGATCCTCGTC
ACCGGCCCCACCGGCAGCGGCAAGACCACCACGCTGTACTCCTTGCTCGCGCATCTCAATACCGAGGCGCGAAGCATCAT
GACGCTGGAGGATCCGGTCGAATATCCGATGCCGCGGCTACGCCAGACCCAGGTCGCCGACAGCGGGCGCATCGGCTTCG
CCGAAGGCGTGCGGGCAATGCTGCGGCAGGACCCGGATGTACTGCTGATCGGCGAAATCCGCGATGCCGACACCGCGGCC
ATGGCGCTGCGGGCGGCAGTCACCGGTCATCAGGTGTATTCGACGCTGCACGCCGGCTCGGCGCTCGGCGCGCTGCCCCG
GCTGCGCGAGCTGGGGGTGTCGCCCGAAATGCTGGCGGGCAACCTCATCGGCATCGTCGCCCAACGCCTGGTGCGCCGCC
TTTGCCCGGACTGCGCTGAAACAGTGGCGATCGAGCCATGGGCGCGCCCCTACCTCGCCACCGACGACAGCGCAGCCGCC
ACCCGGATGCAGGCCCGCGGCTGTGCCCGCTGCAACTTCCAGGGCTATCGCGGCCGGCTCGCGCTGATGGAAATCCTGCC
GGTCGACACGGGGCTGGACGACCTCCTCGCCCGCGGCGCCAACCGTTCCGACCTGCTCGCCCATGCGCAGCGTCAGGGCC
ATGTCGGGCTTGCCGCTGCGGGGCTCGCCCGGGTGCGGGCCGGAGACACCACGCTGGAAGAACTGCTGCGCGTCGTCGAC
GTCCGTGGAGCCCGGCGATGA

Upstream 100 bases:

>100_bases
ATGCGCGCGCCCTCGAAGCAGCCTTGCTGCGGCCGCACAGCTTCGACCCGGAACAGGCCCGCGGGCGCCTGCACGCCCTC
ACCGCAACGCGATGAACGCC

Downstream 100 bases:

>100_bases
GCAGACTGCAGGCCCATGCCTATCGCGCGGTCAACGGCGACGGCCGCCGGGTGCGCGGTCGGGCCCACGCGCTGCACCTC
AACGATCTCGAAGCACGGCT

Product: general secretion pathway protein E

Products: NA

Alternate protein names: Cholera toxin secretion protein epsE; Type II traffic warden ATPase [H]

Number of amino acids: Translated: 566; Mature: 565

Protein sequence:

>566_residues
MAPLPDHDALATLLLAAGVIGQDQLRIARMEQQRAPRPLADLLLGLGFLSESALRGVISEASGRRPVDLARSLPDDAALA
LVPVEFARRNQLLPLSLDTDQAVLTIATAATDDRAVIDKLLALCPQLRRVECLLAAGPELGRAIERHYGQAQSLESILHE
LETGAPPPPQRPGGEGHPQALVRLVDALLLDAVKRDASDIHFEPEAHHLRIRCRIDGVLRQTRALHATYWPAMAVRIKVL
AGLNIAETRVPQDGRFSFDVAGHAIDFRVSCMPTLHGENVVLRLLDRSKGVVPLSDLGLGAGDLAALEAMIARPEGLILV
TGPTGSGKTTTLYSLLAHLNTEARSIMTLEDPVEYPMPRLRQTQVADSGRIGFAEGVRAMLRQDPDVLLIGEIRDADTAA
MALRAAVTGHQVYSTLHAGSALGALPRLRELGVSPEMLAGNLIGIVAQRLVRRLCPDCAETVAIEPWARPYLATDDSAAA
TRMQARGCARCNFQGYRGRLALMEILPVDTGLDDLLARGANRSDLLAHAQRQGHVGLAAAGLARVRAGDTTLEELLRVVD
VRGARR

Sequences:

>Translated_566_residues
MAPLPDHDALATLLLAAGVIGQDQLRIARMEQQRAPRPLADLLLGLGFLSESALRGVISEASGRRPVDLARSLPDDAALA
LVPVEFARRNQLLPLSLDTDQAVLTIATAATDDRAVIDKLLALCPQLRRVECLLAAGPELGRAIERHYGQAQSLESILHE
LETGAPPPPQRPGGEGHPQALVRLVDALLLDAVKRDASDIHFEPEAHHLRIRCRIDGVLRQTRALHATYWPAMAVRIKVL
AGLNIAETRVPQDGRFSFDVAGHAIDFRVSCMPTLHGENVVLRLLDRSKGVVPLSDLGLGAGDLAALEAMIARPEGLILV
TGPTGSGKTTTLYSLLAHLNTEARSIMTLEDPVEYPMPRLRQTQVADSGRIGFAEGVRAMLRQDPDVLLIGEIRDADTAA
MALRAAVTGHQVYSTLHAGSALGALPRLRELGVSPEMLAGNLIGIVAQRLVRRLCPDCAETVAIEPWARPYLATDDSAAA
TRMQARGCARCNFQGYRGRLALMEILPVDTGLDDLLARGANRSDLLAHAQRQGHVGLAAAGLARVRAGDTTLEELLRVVD
VRGARR
>Mature_565_residues
APLPDHDALATLLLAAGVIGQDQLRIARMEQQRAPRPLADLLLGLGFLSESALRGVISEASGRRPVDLARSLPDDAALAL
VPVEFARRNQLLPLSLDTDQAVLTIATAATDDRAVIDKLLALCPQLRRVECLLAAGPELGRAIERHYGQAQSLESILHEL
ETGAPPPPQRPGGEGHPQALVRLVDALLLDAVKRDASDIHFEPEAHHLRIRCRIDGVLRQTRALHATYWPAMAVRIKVLA
GLNIAETRVPQDGRFSFDVAGHAIDFRVSCMPTLHGENVVLRLLDRSKGVVPLSDLGLGAGDLAALEAMIARPEGLILVT
GPTGSGKTTTLYSLLAHLNTEARSIMTLEDPVEYPMPRLRQTQVADSGRIGFAEGVRAMLRQDPDVLLIGEIRDADTAAM
ALRAAVTGHQVYSTLHAGSALGALPRLRELGVSPEMLAGNLIGIVAQRLVRRLCPDCAETVAIEPWARPYLATDDSAAAT
RMQARGCARCNFQGYRGRLALMEILPVDTGLDDLLARGANRSDLLAHAQRQGHVGLAAAGLARVRAGDTTLEELLRVVDV
RGARR

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=378, Percent_Identity=46.031746031746, Blast_Score=332, Evalue=4e-92,
Organism=Escherichia coli, GI1786296, Length=391, Percent_Identity=43.2225063938619, Blast_Score=288, Evalue=5e-79,
Organism=Escherichia coli, GI87082188, Length=210, Percent_Identity=39.5238095238095, Blast_Score=110, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 60865; Mature: 60734

Theoretical pI: Translated: 6.92; Mature: 6.92

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAPLPDHDALATLLLAAGVIGQDQLRIARMEQQRAPRPLADLLLGLGFLSESALRGVISE
CCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
ASGRRPVDLARSLPDDAALALVPVEFARRNQLLPLSLDTDQAVLTIATAATDDRAVIDKL
CCCCCCHHHHHCCCCCCEEEEEEHHHHHCCCEEEEECCCCCEEEEEEECCCCCHHHHHHH
LALCPQLRRVECLLAAGPELGRAIERHYGQAQSLESILHELETGAPPPPQRPGGEGHPQA
HHHCHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHH
LVRLVDALLLDAVKRDASDIHFEPEAHHLRIRCRIDGVLRQTRALHATYWPAMAVRIKVL
HHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHH
AGLNIAETRVPQDGRFSFDVAGHAIDFRVSCMPTLHGENVVLRLLDRSKGVVPLSDLGLG
HCCCHHHHCCCCCCCEEEEECCCEEEEEEEEEECCCCCCHHHEEECCCCCCCCHHHCCCC
AGDLAALEAMIARPEGLILVTGPTGSGKTTTLYSLLAHLNTEARSIMTLEDPVEYPMPRL
CHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHCCHHHHEEEECCCCCCCCCHH
RQTQVADSGRIGFAEGVRAMLRQDPDVLLIGEIRDADTAAMALRAAVTGHQVYSTLHAGS
HHHHCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCH
ALGALPRLRELGVSPEMLAGNLIGIVAQRLVRRLCPDCAETVAIEPWARPYLATDDSAAA
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHEECCCCCCCCCCCCCHHHH
TRMQARGCARCNFQGYRGRLALMEILPVDTGLDDLLARGANRSDLLAHAQRQGHVGLAAA
HHHHHCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHCCCCHHHHHHHHHHCCCCCHHHH
GLARVRAGDTTLEELLRVVDVRGARR
HHHHHCCCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
APLPDHDALATLLLAAGVIGQDQLRIARMEQQRAPRPLADLLLGLGFLSESALRGVISE
CCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
ASGRRPVDLARSLPDDAALALVPVEFARRNQLLPLSLDTDQAVLTIATAATDDRAVIDKL
CCCCCCHHHHHCCCCCCEEEEEEHHHHHCCCEEEEECCCCCEEEEEEECCCCCHHHHHHH
LALCPQLRRVECLLAAGPELGRAIERHYGQAQSLESILHELETGAPPPPQRPGGEGHPQA
HHHCHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHH
LVRLVDALLLDAVKRDASDIHFEPEAHHLRIRCRIDGVLRQTRALHATYWPAMAVRIKVL
HHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHH
AGLNIAETRVPQDGRFSFDVAGHAIDFRVSCMPTLHGENVVLRLLDRSKGVVPLSDLGLG
HCCCHHHHCCCCCCCEEEEECCCEEEEEEEEEECCCCCCHHHEEECCCCCCCCHHHCCCC
AGDLAALEAMIARPEGLILVTGPTGSGKTTTLYSLLAHLNTEARSIMTLEDPVEYPMPRL
CHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHCCHHHHEEEECCCCCCCCCHH
RQTQVADSGRIGFAEGVRAMLRQDPDVLLIGEIRDADTAAMALRAAVTGHQVYSTLHAGS
HHHHCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCH
ALGALPRLRELGVSPEMLAGNLIGIVAQRLVRRLCPDCAETVAIEPWARPYLATDDSAAA
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHEECCCCCCCCCCCCCHHHH
TRMQARGCARCNFQGYRGRLALMEILPVDTGLDDLLARGANRSDLLAHAQRQGHVGLAAA
HHHHHCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHCCCCHHHHHHHHHHCCCCCHHHH
GLARVRAGDTTLEELLRVVDVRGARR
HHHHHCCCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8423007; 10952301 [H]