Click here to switch to the map view.

The map label for this gene is amiC2 [H]

Identifier: 119896965

GI number: 119896965

Start: 709010

End: 710266

Strand: Direct

Name: amiC2 [H]

Synonym: azo0674

Alternate gene names: 119896965

Gene position: 709010-710266 (Clockwise)

Preceding gene: 119896959

Following gene: 119896966

Centisome position: 16.2

GC content: 63.56

Gene sequence:

>1257_bases
ATGCAAACCCGTCGCAGCTTCATCAAGGCCCTCGCGCTGTCCGCTTCCATCGCCGCGATCGGCGCGCCGTTCGGCGCCCA
CGCCGCCGACACCATCAAGGTCGGCATCCTGCATTCGCTGTCGGGCACGATGGCGATCTCCGAGACCGCGCTGAAGAACG
TCGCGCTGATGACGATCGAGGAAATCAACGCCAAGGGCGGCGTGATGGGCAAGAAGCTGGAGCCGGTGGTGGTCGACCCG
GCGTCGAACTGGCCGTTGTTCGCCGAGAAGGCCCGCCAGCTGGTTGCCCAGGACAAGGTCGCCGCGGTGTTCGGCTGCTG
GACCTCGGTCTCCCGCAAGTCGGTGAACCCGGTGTTCAAGGAATTGAACGGCCTGCTGTTCTACCCGGTGCAGTACGAAG
GCGAAGAGCTGGAGAAGAACGTGTTCTACACCGGCGCCGCGCCCAACCAGCAGGCCATCCCGGCGGTGGAATACCTGATG
AGCGCCGAAGGCGGCAGCGCCAAGCGCTTCGTGCTGCTGGGCACCGACTACGTGTACCCGCGCACCACCAACAAGATCCT
GCGCGCCTTCCTCAAGAGCAAGGGCGTGGCCGATGCGGACATCATGGAGGACTACACCCCCTTCGGCCATTCGGACTACC
AGACCATCATCGCCAACATCAAGAAGTTCGCCTCCGAAGGCAAGAAGACGGCGGTGATCTCCACCATCAACGGCGACTCC
AACGTGCCGTTCTACAAGGAACTGGGCAACGCCGGCCTGAAGGCGACCGACGTGCCGGTGGTGGCGTTCTCGGTGGGTGA
AGAGGAACTGCGCGGCGTCGATACCAAGCCGCTGGTCGGCCACCTCGCCGCGTGGAACTACTTCATGACCGTGAAGAACC
CGGAAAACACCGCCTTCATCAAGAAGTACAAGGACTGGGCGAAGAAGAACGGCGTGCCCAACGCCGACACCGTGGTCACC
AACGACCCGATGGAAGCCACCTACGTCGGCATCTACATGTGGAAGCAGGCGGTCGAGAAGGCCAAGTCCACCGACACCGA
CAAGGTCATCGCCGCGCTCGGCGGCCAGACCTTCAAGGCGCCGTCGGGCTTCACGCTGACCATGGACAAGACCAACCACC
ACCTGCACAAGCCGGTGTTCATCGGCGAAGTGCGCGCCGACGGCCAGTTCGACGTGGTGTGGAAGACCAAGGAGCCGATC
CGCGCCCAGCCGTGGAGCCCGTTCATCCCGGGTAACGAAGGCAAGCAGGGGCTTTGA

Upstream 100 bases:

>100_bases
TTGCGTATTCCGGTGCCGCCGCCCGACGCCTAATCTCGACTCGTGCACTGCAACGCGGCCCCGGCCGCATCCAGAACCGT
CCTAGACCAGGGAGACCTAC

Downstream 100 bases:

>100_bases
GGTAGGGTTTTCGCTTTGTCCCTCCCCCTTCAAGGGGGGCGGAGCCGGGGTTTCTGCAAGCATGCTTGCCGCCGGTGTAG
CGGCGAGGCGAAGCCGAGAC

Product: putative aliphatic amidase expression-regulating protein

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 418; Mature: 418

Protein sequence:

>418_residues
MQTRRSFIKALALSASIAAIGAPFGAHAADTIKVGILHSLSGTMAISETALKNVALMTIEEINAKGGVMGKKLEPVVVDP
ASNWPLFAEKARQLVAQDKVAAVFGCWTSVSRKSVNPVFKELNGLLFYPVQYEGEELEKNVFYTGAAPNQQAIPAVEYLM
SAEGGSAKRFVLLGTDYVYPRTTNKILRAFLKSKGVADADIMEDYTPFGHSDYQTIIANIKKFASEGKKTAVISTINGDS
NVPFYKELGNAGLKATDVPVVAFSVGEEELRGVDTKPLVGHLAAWNYFMTVKNPENTAFIKKYKDWAKKNGVPNADTVVT
NDPMEATYVGIYMWKQAVEKAKSTDTDKVIAALGGQTFKAPSGFTLTMDKTNHHLHKPVFIGEVRADGQFDVVWKTKEPI
RAQPWSPFIPGNEGKQGL

Sequences:

>Translated_418_residues
MQTRRSFIKALALSASIAAIGAPFGAHAADTIKVGILHSLSGTMAISETALKNVALMTIEEINAKGGVMGKKLEPVVVDP
ASNWPLFAEKARQLVAQDKVAAVFGCWTSVSRKSVNPVFKELNGLLFYPVQYEGEELEKNVFYTGAAPNQQAIPAVEYLM
SAEGGSAKRFVLLGTDYVYPRTTNKILRAFLKSKGVADADIMEDYTPFGHSDYQTIIANIKKFASEGKKTAVISTINGDS
NVPFYKELGNAGLKATDVPVVAFSVGEEELRGVDTKPLVGHLAAWNYFMTVKNPENTAFIKKYKDWAKKNGVPNADTVVT
NDPMEATYVGIYMWKQAVEKAKSTDTDKVIAALGGQTFKAPSGFTLTMDKTNHHLHKPVFIGEVRADGQFDVVWKTKEPI
RAQPWSPFIPGNEGKQGL
>Mature_418_residues
MQTRRSFIKALALSASIAAIGAPFGAHAADTIKVGILHSLSGTMAISETALKNVALMTIEEINAKGGVMGKKLEPVVVDP
ASNWPLFAEKARQLVAQDKVAAVFGCWTSVSRKSVNPVFKELNGLLFYPVQYEGEELEKNVFYTGAAPNQQAIPAVEYLM
SAEGGSAKRFVLLGTDYVYPRTTNKILRAFLKSKGVADADIMEDYTPFGHSDYQTIIANIKKFASEGKKTAVISTINGDS
NVPFYKELGNAGLKATDVPVVAFSVGEEELRGVDTKPLVGHLAAWNYFMTVKNPENTAFIKKYKDWAKKNGVPNADTVVT
NDPMEATYVGIYMWKQAVEKAKSTDTDKVIAALGGQTFKAPSGFTLTMDKTNHHLHKPVFIGEVRADGQFDVVWKTKEPI
RAQPWSPFIPGNEGKQGL

Specific function: Negatively regulates the expression of the aliphatic amidase operon. AmiC functions by inhibiting the action of AmiR at the protein level. It exhibits protein kinase activity [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000709 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 45532; Mature: 45532

Theoretical pI: Translated: 9.35; Mature: 9.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTRRSFIKALALSASIAAIGAPFGAHAADTIKVGILHSLSGTMAISETALKNVALMTIE
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCEEHHHHHHHHHEEEHH
EINAKGGVMGKKLEPVVVDPASNWPLFAEKARQLVAQDKVAAVFGCWTSVSRKSVNPVFK
HHCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
ELNGLLFYPVQYEGEELEKNVFYTGAAPNQQAIPAVEYLMSAEGGSAKRFVLLGTDYVYP
HCCCEEEEEEECCCCHHCCCEEEECCCCCCCCCHHHHHHHHCCCCCCEEEEEEECCEECC
RTTNKILRAFLKSKGVADADIMEDYTPFGHSDYQTIIANIKKFASEGKKTAVISTINGDS
CCHHHHHHHHHHHCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCC
NVPFYKELGNAGLKATDVPVVAFSVGEEELRGVDTKPLVGHLAAWNYFMTVKNPENTAFI
CCCHHHHHCCCCCEECCCCEEEEECCHHHHCCCCCCHHHHHHHHEEEEEEEECCCCCHHH
KKYKDWAKKNGVPNADTVVTNDPMEATYVGIYMWKQAVEKAKSTDTDKVIAALGGQTFKA
HHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCEEEC
PSGFTLTMDKTNHHLHKPVFIGEVRADGQFDVVWKTKEPIRAQPWSPFIPGNEGKQGL
CCCCEEEEECCCCCCCCCEEEEEEECCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MQTRRSFIKALALSASIAAIGAPFGAHAADTIKVGILHSLSGTMAISETALKNVALMTIE
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCEEHHHHHHHHHEEEHH
EINAKGGVMGKKLEPVVVDPASNWPLFAEKARQLVAQDKVAAVFGCWTSVSRKSVNPVFK
HHCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
ELNGLLFYPVQYEGEELEKNVFYTGAAPNQQAIPAVEYLMSAEGGSAKRFVLLGTDYVYP
HCCCEEEEEEECCCCHHCCCEEEECCCCCCCCCHHHHHHHHCCCCCCEEEEEEECCEECC
RTTNKILRAFLKSKGVADADIMEDYTPFGHSDYQTIIANIKKFASEGKKTAVISTINGDS
CCHHHHHHHHHHHCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCC
NVPFYKELGNAGLKATDVPVVAFSVGEEELRGVDTKPLVGHLAAWNYFMTVKNPENTAFI
CCCHHHHHCCCCCEECCCCEEEEECCHHHHCCCCCCHHHHHHHHEEEEEEEECCCCCHHH
KKYKDWAKKNGVPNADTVVTNDPMEATYVGIYMWKQAVEKAKSTDTDKVIAALGGQTFKA
HHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCEEEC
PSGFTLTMDKTNHHLHKPVFIGEVRADGQFDVVWKTKEPIRAQPWSPFIPGNEGKQGL
CCCCEEEEECCCCCCCCCEEEEEEECCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1907262; 10984043; 1762155; 7813419; 10508151; 10708652 [H]