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The map label for this gene is braD [H]

Identifier: 119896966

GI number: 119896966

Start: 710535

End: 712166

Strand: Direct

Name: braD [H]

Synonym: azo0675

Alternate gene names: 119896966

Gene position: 710535-712166 (Clockwise)

Preceding gene: 119896965

Following gene: 119896967

Centisome position: 16.24

GC content: 70.59

Gene sequence:

>1632_bases
ATGGAGCCTGCCATGAACCGATTGCTGCTGCGGCTCGCGCTGCTCTTCGCCTCGCTCGCCTGCCTTGCCCTGCCGGCCCG
TGCCGCGCTCGATCCCGCCCTGGTGGCCGGGTTCGCCGGCGATTTCAACAGCCGCGTCGCCGCCATCGAGGCGTTGGGCG
TGGAGGGCAGCGCCGAGGCGCGCGCGCTGCTCGATGCGGTGGAGGCCGGCAATCTCGGCGTCGCCGCCGACAAGGTGGTC
GTCATCGATGGCGACGCGGTCAAGGACGCCGCCAGCGGCGAAGCCCTGCAGATCCCCGCCGACGAGGTCGAGACGATCAC
GGTGAACAACCGCATCCGCCGCGCGCTCGCCACCGCGCACGCCGCGCTGGCGCTGTCGGCGGAGAGCGCGGCCGATCGCC
TCGCTGCCGCCAACACGCTGACCGAGAACACCTCCGAATCGCTGCTGCCGCTGTTCGAACGCACGCTCGCCGGGGAAAAG
GACGAAGCCGTGCGCGGCGTGCTCAGCCGCGCGGTCGCCCGCGTCAACCTCGGCTCCGCCGACGCCGCCAAGCGCCTGAA
GGCCGCCGAGGCGCTCGGCGAATCCTCCGATCCGGCGGTAAAGAACATGCTCGCCGCGCTGCTGGAAAAGCGCGGCGAGG
GCAAGGACGCCAGCTGGGCCGAACCCGACGCCGAGGTGCGCGCCGCGGCCGAAGCCTCGATCAAGGCCATCGACCGTCGC
ATCGCCACCGCGCAGGCGGTGGGCACGGTGTTCTCCGGCCTGTCGCTCGGCTCCATCCTGCTGCTGGCCGCGCTCGGCCT
CGCGATCACCTACGGCGTGATGGGCATCATCAACATGGCCCACGGCGAACTGCTGATGGTCGGCGCCTACGCCACCTTCC
TGGTGCAGGGCCTGTTCCGCAGCTACCTGCCGGCCTACCTCGACTGGTACGTGGTCGCGGCCATCCCGGTCGCCTTCTTC
GCCGCCGCGCTGGTCGGCGTGGCGATGGAACGGCTGGTGCTGCGCCACCTCTACGGCCGCCCGCTGGAATCGCTGCTCGC
CACGTGGGGCCTGTCGCTGGTGCTGATTCAGGCTGCGCGCGTGGTGTTCGGTCCGCAGAACCTCGAACTCGCCAATCCGG
GCTGGATGTCGGGCGGCATGGAACTCGCCGCGGGCGTGGTGCTGCCCTACAACCGCATCGTCATCATCGGCTTCGCGGTG
TTCGTGCTGGTGCTGATCTGGCTGATGATGCAGAAGACCCGGCTCGGCATGTTCGTGCGCGCCGTGACGCAGAACCGCCC
GATGGCCGGCTGCGTCGGCGTGCCGACGGCGCGGGTCGATACGCTCGCCTTCGCGATCGGCTCCGGCGTGGCCGGGCTGG
GCGGGCTGGCGCTGTCGCAGATCGCCAACGTCGGCCCGGCGATGGGCCAGGGCTACATCGTCGATGCCTTCATGGTCGTG
GTGCTCGGCGGGGTCGGCCAGCTGGCCGGCGCGGTGTGGGCGGCGCTCGGGCTGGGCATCGTCGCCAAGTTCCTCGAAGG
CTGGGCCGGCGCGGTGATCGCCAAGATCCTGGTGCTGGTGTTCATCATCGTCTTCATCCAGAAGCGCCCGCAGGGGATCT
TCGCCCTCAAGGGACGCTTCGCCGACAGCTGA

Upstream 100 bases:

>100_bases
AGGGGAGGGAGTTTCACCTCCCGCCGGCTGCGGCGTTCGTCCGACACCGGCCGCTTGTTTGCCCCGAATTCCCGATTCGC
CCCCGCGGGCGAATGATCCG

Downstream 100 bases:

>100_bases
GACAGCAAGCCCCGACCACCGCAGCGACGGTCGGGGTGTTCGCGCAGCGGGCGAGGACTGTCCGAGCGAACGCAGTGAGC
GAGTTCCGCAGCCCGCGGAG

Product: ABC transporter permease

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 543; Mature: 543

Protein sequence:

>543_residues
MEPAMNRLLLRLALLFASLACLALPARAALDPALVAGFAGDFNSRVAAIEALGVEGSAEARALLDAVEAGNLGVAADKVV
VIDGDAVKDAASGEALQIPADEVETITVNNRIRRALATAHAALALSAESAADRLAAANTLTENTSESLLPLFERTLAGEK
DEAVRGVLSRAVARVNLGSADAAKRLKAAEALGESSDPAVKNMLAALLEKRGEGKDASWAEPDAEVRAAAEASIKAIDRR
IATAQAVGTVFSGLSLGSILLLAALGLAITYGVMGIINMAHGELLMVGAYATFLVQGLFRSYLPAYLDWYVVAAIPVAFF
AAALVGVAMERLVLRHLYGRPLESLLATWGLSLVLIQAARVVFGPQNLELANPGWMSGGMELAAGVVLPYNRIVIIGFAV
FVLVLIWLMMQKTRLGMFVRAVTQNRPMAGCVGVPTARVDTLAFAIGSGVAGLGGLALSQIANVGPAMGQGYIVDAFMVV
VLGGVGQLAGAVWAALGLGIVAKFLEGWAGAVIAKILVLVFIIVFIQKRPQGIFALKGRFADS

Sequences:

>Translated_543_residues
MEPAMNRLLLRLALLFASLACLALPARAALDPALVAGFAGDFNSRVAAIEALGVEGSAEARALLDAVEAGNLGVAADKVV
VIDGDAVKDAASGEALQIPADEVETITVNNRIRRALATAHAALALSAESAADRLAAANTLTENTSESLLPLFERTLAGEK
DEAVRGVLSRAVARVNLGSADAAKRLKAAEALGESSDPAVKNMLAALLEKRGEGKDASWAEPDAEVRAAAEASIKAIDRR
IATAQAVGTVFSGLSLGSILLLAALGLAITYGVMGIINMAHGELLMVGAYATFLVQGLFRSYLPAYLDWYVVAAIPVAFF
AAALVGVAMERLVLRHLYGRPLESLLATWGLSLVLIQAARVVFGPQNLELANPGWMSGGMELAAGVVLPYNRIVIIGFAV
FVLVLIWLMMQKTRLGMFVRAVTQNRPMAGCVGVPTARVDTLAFAIGSGVAGLGGLALSQIANVGPAMGQGYIVDAFMVV
VLGGVGQLAGAVWAALGLGIVAKFLEGWAGAVIAKILVLVFIIVFIQKRPQGIFALKGRFADS
>Mature_543_residues
MEPAMNRLLLRLALLFASLACLALPARAALDPALVAGFAGDFNSRVAAIEALGVEGSAEARALLDAVEAGNLGVAADKVV
VIDGDAVKDAASGEALQIPADEVETITVNNRIRRALATAHAALALSAESAADRLAAANTLTENTSESLLPLFERTLAGEK
DEAVRGVLSRAVARVNLGSADAAKRLKAAEALGESSDPAVKNMLAALLEKRGEGKDASWAEPDAEVRAAAEASIKAIDRR
IATAQAVGTVFSGLSLGSILLLAALGLAITYGVMGIINMAHGELLMVGAYATFLVQGLFRSYLPAYLDWYVVAAIPVAFF
AAALVGVAMERLVLRHLYGRPLESLLATWGLSLVLIQAARVVFGPQNLELANPGWMSGGMELAAGVVLPYNRIVIIGFAV
FVLVLIWLMMQKTRLGMFVRAVTQNRPMAGCVGVPTARVDTLAFAIGSGVAGLGGLALSQIANVGPAMGQGYIVDAFMVV
VLGGVGQLAGAVWAALGLGIVAKFLEGWAGAVIAKILVLVFIIVFIQKRPQGIFALKGRFADS

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0559

COG function: function code E; Branched-chain amino acid ABC-type transport system, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789866, Length=293, Percent_Identity=29.6928327645051, Blast_Score=84, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851 [H]

Pfam domain/function: PF02653 BPD_transp_2 [H]

EC number: NA

Molecular weight: Translated: 56370; Mature: 56370

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEPAMNRLLLRLALLFASLACLALPARAALDPALVAGFAGDFNSRVAAIEALGVEGSAEA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCHHHHHHHHCCCCCHHH
RALLDAVEAGNLGVAADKVVVIDGDAVKDAASGEALQIPADEVETITVNNRIRRALATAH
HHHHHHHHCCCCCEEECEEEEECCCHHHHCCCCCEEECCCCCCCEEEHHHHHHHHHHHHH
AALALSAESAADRLAAANTLTENTSESLLPLFERTLAGEKDEAVRGVLSRAVARVNLGSA
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCH
DAAKRLKAAEALGESSDPAVKNMLAALLEKRGEGKDASWAEPDAEVRAAAEASIKAIDRR
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
IATAQAVGTVFSGLSLGSILLLAALGLAITYGVMGIINMAHGELLMVGAYATFLVQGLFR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHH
SYLPAYLDWYVVAAIPVAFFAAALVGVAMERLVLRHLYGRPLESLLATWGLSLVLIQAAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
VVFGPQNLELANPGWMSGGMELAAGVVLPYNRIVIIGFAVFVLVLIWLMMQKTRLGMFVR
HHCCCCCCCCCCCCCCCCCHHHHHCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVTQNRPMAGCVGVPTARVDTLAFAIGSGVAGLGGLALSQIANVGPAMGQGYIVDAFMVV
HHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
VLGGVGQLAGAVWAALGLGIVAKFLEGWAGAVIAKILVLVFIIVFIQKRPQGIFALKGRF
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCC
ADS
CCC
>Mature Secondary Structure
MEPAMNRLLLRLALLFASLACLALPARAALDPALVAGFAGDFNSRVAAIEALGVEGSAEA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCHHHHHHHHCCCCCHHH
RALLDAVEAGNLGVAADKVVVIDGDAVKDAASGEALQIPADEVETITVNNRIRRALATAH
HHHHHHHHCCCCCEEECEEEEECCCHHHHCCCCCEEECCCCCCCEEEHHHHHHHHHHHHH
AALALSAESAADRLAAANTLTENTSESLLPLFERTLAGEKDEAVRGVLSRAVARVNLGSA
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCH
DAAKRLKAAEALGESSDPAVKNMLAALLEKRGEGKDASWAEPDAEVRAAAEASIKAIDRR
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
IATAQAVGTVFSGLSLGSILLLAALGLAITYGVMGIINMAHGELLMVGAYATFLVQGLFR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHH
SYLPAYLDWYVVAAIPVAFFAAALVGVAMERLVLRHLYGRPLESLLATWGLSLVLIQAAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
VVFGPQNLELANPGWMSGGMELAAGVVLPYNRIVIIGFAVFVLVLIWLMMQKTRLGMFVR
HHCCCCCCCCCCCCCCCCCHHHHHCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVTQNRPMAGCVGVPTARVDTLAFAIGSGVAGLGGLALSQIANVGPAMGQGYIVDAFMVV
HHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
VLGGVGQLAGAVWAALGLGIVAKFLEGWAGAVIAKILVLVFIIVFIQKRPQGIFALKGRF
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCC
ADS
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043; 2509433 [H]