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The map label for this gene is napB1 [H]

Identifier: 119896961

GI number: 119896961

Start: 705250

End: 705717

Strand: Reverse

Name: napB1 [H]

Synonym: azo0670

Alternate gene names: 119896961

Gene position: 705717-705250 (Counterclockwise)

Preceding gene: 119896962

Following gene: 119896960

Centisome position: 16.13

GC content: 68.38

Gene sequence:

>468_bases
ATGCGCATCCTGCTTCCCTTCGCGCTGGCTGCCGCCTTCGCCGGCGCCGCGCTCGCCCAGCATGTCGACAACGCCCGCGG
CCCCACGCCGCTGATGGAGGAACGCACGCCACCGCCGCTGCAGAACCCGGACAACAGCGACGTGCGCCGCCAGCGCGCCT
ACGCCATGCAGCCGCCGATCATTCCGCACAAGATCGAGGGCTACCAGCTCGACCGCAACGTCAATCGCTGCATGATGTGC
CACGCCCGCACCCGCACCGAGCAGAGCCAGGCGCCGATGATCAGCGTGTCCCACTTCACCGACCGCGACGGCAACTTCCT
CGCCGAGCTGTCGCCGCGGCGTTATTTCTGCCTGCAGTGCCACGTGCCCCAGGTGCCGGTCAATCCGATCGTGGAGAACC
GCTTCATCGACGTCGAAACCATGCTCAGGCGCGGCGGCTCCGCCTCCACGCTGCCCGAGCAACGCTGA

Upstream 100 bases:

>100_bases
CTGATCAACAAGGTGACGCTGGACGCGACCTGCCCGATCTCGCTGCAGACGGACTTCAAGAAGTGCGCGGTGAACATCCG
CAAGGTCTAGGAGAACAGCC

Downstream 100 bases:

>100_bases
GCGGAGGCGCCCATGTTCGGCCTTCTGAAGCGGTACTGGCGGGTGATCCGCCGCCCCAGCACGCACTACAGCCTGGGTCT
GCTGGTGATCGGCGGCTTTG

Product: diheme cytochrome C

Products: reduced acceptor; NO3- [C]

Alternate protein names: NA

Number of amino acids: Translated: 155; Mature: 155

Protein sequence:

>155_residues
MRILLPFALAAAFAGAALAQHVDNARGPTPLMEERTPPPLQNPDNSDVRRQRAYAMQPPIIPHKIEGYQLDRNVNRCMMC
HARTRTEQSQAPMISVSHFTDRDGNFLAELSPRRYFCLQCHVPQVPVNPIVENRFIDVETMLRRGGSASTLPEQR

Sequences:

>Translated_155_residues
MRILLPFALAAAFAGAALAQHVDNARGPTPLMEERTPPPLQNPDNSDVRRQRAYAMQPPIIPHKIEGYQLDRNVNRCMMC
HARTRTEQSQAPMISVSHFTDRDGNFLAELSPRRYFCLQCHVPQVPVNPIVENRFIDVETMLRRGGSASTLPEQR
>Mature_155_residues
MRILLPFALAAAFAGAALAQHVDNARGPTPLMEERTPPPLQNPDNSDVRRQRAYAMQPPIIPHKIEGYQLDRNVNRCMMC
HARTRTEQSQAPMISVSHFTDRDGNFLAELSPRRYFCLQCHVPQVPVNPIVENRFIDVETMLRRGGSASTLPEQR

Specific function: Small subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme NapC protein, thus allowing electron flow between membrane and periplasm.

COG id: COG3043

COG function: function code C; Nitrate reductase cytochrome c-type subunit

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87082065, Length=128, Percent_Identity=44.53125, Blast_Score=107, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011031
- InterPro:   IPR005591 [H]

Pfam domain/function: PF03892 NapB [H]

EC number: NA

Molecular weight: Translated: 17545; Mature: 17545

Theoretical pI: Translated: 8.67; Mature: 8.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
7.1 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
7.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRILLPFALAAAFAGAALAQHVDNARGPTPLMEERTPPPLQNPDNSDVRRQRAYAMQPPI
CEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCC
IPHKIEGYQLDRNVNRCMMCHARTRTEQSQAPMISVSHFTDRDGNFLAELSPRRYFCLQC
CCCCCCCEEECCCCCHHEEHHHHHCCCCCCCCEEEEECCCCCCCCEEEEECCCEEEEEEE
HVPQVPVNPIVENRFIDVETMLRRGGSASTLPEQR
CCCCCCCCCCCCCCEECHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MRILLPFALAAAFAGAALAQHVDNARGPTPLMEERTPPPLQNPDNSDVRRQRAYAMQPPI
CEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCC
IPHKIEGYQLDRNVNRCMMCHARTRTEQSQAPMISVSHFTDRDGNFLAELSPRRYFCLQC
CCCCCCCEEECCCCCHHEEHHHHHCCCCCCCCEEEEECCCCCCCCEEEEECCCEEEEEEE
HVPQVPVNPIVENRFIDVETMLRRGGSASTLPEQR
CCCCCCCCCCCCCCEECHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Acceptor; nitrite [C]

Specific reaction: Acceptor + nitrite = reduced acceptor + NO3- [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8376334; 12948488 [H]