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The map label for this gene is htrB [H]

Identifier: 119896881

GI number: 119896881

Start: 621534

End: 622355

Strand: Direct

Name: htrB [H]

Synonym: azo0590

Alternate gene names: 119896881

Gene position: 621534-622355 (Clockwise)

Preceding gene: 119896879

Following gene: 119896882

Centisome position: 14.2

GC content: 72.14

Gene sequence:

>822_bases
TTGCCGCTGTCGTGGCTGCACCGCCTGGGCGGCTGGATGGGCTGGCTCGTCTATTGGTGCGCCCCCGGCTACCGCCGGCG
CCTGCGGCGCAACCTCGCACTCGCCACCGGGCGCGAGGACCGCGCCCTGCTGCATGCCGCGGTGGCGGAAGCGGGGCGGC
AGGCGCTGGAGCTGCCCTGGCTGTGGCTGCGGCCGCTCGACCAGGTCGTCGCCAAGGTGGTGCGGGTGGAAGGATGGGAG
CTGGTCGAGGCCGCCGAGCGGGCCGGCGCCGGCATCCTGTTCTTCACGCCGCACCTGGGCTGCTTCGAGATCACCGCTAC
CTGCTACGCCGCCCACGCCCCGATCACGGTGCTCTACCGCCCGCCGCGCAAGGTTGCGCTGCAGCCGCTGATGGAAGCGG
GCCGCGCGCGCGGCAACATGCGGATCGCACCGGCGGACATCTCGGGCGTGCGTGCGCTGGTGAAGACGCTGCGCAGCAAG
GAGGCGGTGGGGATGCTGCCCGACCAGGTGCCCGCCGCGGGCGAGGGCATGTGGGTGCCGTTCTTCGGCCGTCCGGCCTG
GACGATGACGCTCGGCGCCCGGCTGGCCGAGGTGAAGGGCGTGCACGTCATCTACTGCTGGGCCGAACGCCTGCCGCGCG
GCGAGGGCTACGTGTTCCGCATGTTCGGCCCCACCGAGCCGCTGGAAGGCGACCTCGAAGCGCGCTGCGCCACCATCAAC
CGCGAGATCGAGCGCCTGATCCTGCAATGTCCCGCCCAGTACCTGTGGGGCTACCACCGCTACAAGCGTCCCAAGGGCGC
ACGTGCCAAGGAGGCGCAATGA

Upstream 100 bases:

>100_bases
TATAGGCTGCCGACCCGCGGCGTCAAAGCCGCGCCCGCCACGCAAACTGAAAAATCCCGTAAACGTGCTCGTTTCCCTTC
TTTTTAGACTCTCCTCCCGC

Downstream 100 bases:

>100_bases
GCCCGCTCCAATTCCGTCAGGGAACGCCATGTTGACCCGTCTCGGCGTCGCCGTGTTCTGGCTGCTGCACTGGCTGCCGC
TGCCGCTGCTCGCGCGCATC

Product: lipid A biosynthesis lauroyl acyltransferase

Products: NA

Alternate protein names: Heat shock protein B [H]

Number of amino acids: Translated: 273; Mature: 272

Protein sequence:

>273_residues
MPLSWLHRLGGWMGWLVYWCAPGYRRRLRRNLALATGREDRALLHAAVAEAGRQALELPWLWLRPLDQVVAKVVRVEGWE
LVEAAERAGAGILFFTPHLGCFEITATCYAAHAPITVLYRPPRKVALQPLMEAGRARGNMRIAPADISGVRALVKTLRSK
EAVGMLPDQVPAAGEGMWVPFFGRPAWTMTLGARLAEVKGVHVIYCWAERLPRGEGYVFRMFGPTEPLEGDLEARCATIN
REIERLILQCPAQYLWGYHRYKRPKGARAKEAQ

Sequences:

>Translated_273_residues
MPLSWLHRLGGWMGWLVYWCAPGYRRRLRRNLALATGREDRALLHAAVAEAGRQALELPWLWLRPLDQVVAKVVRVEGWE
LVEAAERAGAGILFFTPHLGCFEITATCYAAHAPITVLYRPPRKVALQPLMEAGRARGNMRIAPADISGVRALVKTLRSK
EAVGMLPDQVPAAGEGMWVPFFGRPAWTMTLGARLAEVKGVHVIYCWAERLPRGEGYVFRMFGPTEPLEGDLEARCATIN
REIERLILQCPAQYLWGYHRYKRPKGARAKEAQ
>Mature_272_residues
PLSWLHRLGGWMGWLVYWCAPGYRRRLRRNLALATGREDRALLHAAVAEAGRQALELPWLWLRPLDQVVAKVVRVEGWEL
VEAAERAGAGILFFTPHLGCFEITATCYAAHAPITVLYRPPRKVALQPLMEAGRARGNMRIAPADISGVRALVKTLRSKE
AVGMLPDQVPAAGEGMWVPFFGRPAWTMTLGARLAEVKGVHVIYCWAERLPRGEGYVFRMFGPTEPLEGDLEARCATINR
EIERLILQCPAQYLWGYHRYKRPKGARAKEAQ

Specific function: Acylates the intermediate (KDO)2-lipid IVA to form (KDO)2-(lauroyl)-lipid IVA. Has 10 fold selectivity for lauroyl- ACP over myristoyl-ACP [H]

COG id: COG1560

COG function: function code M; Lauroyl/myristoyl acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the htrB/msbB family [H]

Homologues:

Organism=Escherichia coli, GI1787292, Length=278, Percent_Identity=26.978417266187, Blast_Score=87, Evalue=9e-19,
Organism=Escherichia coli, GI87082094, Length=249, Percent_Identity=27.3092369477912, Blast_Score=74, Evalue=9e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004960
- InterPro:   IPR011920 [H]

Pfam domain/function: PF03279 Lip_A_acyltrans [H]

EC number: 2.3.1.-

Molecular weight: Translated: 30748; Mature: 30617

Theoretical pI: Translated: 10.13; Mature: 10.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLSWLHRLGGWMGWLVYWCAPGYRRRLRRNLALATGREDRALLHAAVAEAGRQALELPW
CCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCH
LWLRPLDQVVAKVVRVEGWELVEAAERAGAGILFFTPHLGCFEITATCYAAHAPITVLYR
HHHCCHHHHHHHHHHCCCHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHCCCCEEEEEC
PPRKVALQPLMEAGRARGNMRIAPADISGVRALVKTLRSKEAVGMLPDQVPAAGEGMWVP
CCHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHCCHHCCCCCCCCCCCCCCEEEE
FFGRPAWTMTLGARLAEVKGVHVIYCWAERLPRGEGYVFRMFGPTEPLEGDLEARCATIN
ECCCCCHHHHHHHHHHHHCCCEEEEEEHHHCCCCCCEEEEECCCCCCCCCCHHHHHHHHH
REIERLILQCPAQYLWGYHRYKRPKGARAKEAQ
HHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
PLSWLHRLGGWMGWLVYWCAPGYRRRLRRNLALATGREDRALLHAAVAEAGRQALELPW
CHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCH
LWLRPLDQVVAKVVRVEGWELVEAAERAGAGILFFTPHLGCFEITATCYAAHAPITVLYR
HHHCCHHHHHHHHHHCCCHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHCCCCEEEEEC
PPRKVALQPLMEAGRARGNMRIAPADISGVRALVKTLRSKEAVGMLPDQVPAAGEGMWVP
CCHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHCCHHCCCCCCCCCCCCCCEEEE
FFGRPAWTMTLGARLAEVKGVHVIYCWAERLPRGEGYVFRMFGPTEPLEGDLEARCATIN
ECCCCCHHHHHHHHHHHHCCCEEEEEEHHHCCCCCCEEEEECCCCCCCCCCHHHHHHHHH
REIERLILQCPAQYLWGYHRYKRPKGARAKEAQ
HHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Acyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]