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The map label for this gene is msbBa [H]

Identifier: 119896882

GI number: 119896882

Start: 622384

End: 623160

Strand: Direct

Name: msbBa [H]

Synonym: azo0591

Alternate gene names: 119896882

Gene position: 622384-623160 (Clockwise)

Preceding gene: 119896881

Following gene: 119896883

Centisome position: 14.22

GC content: 70.14

Gene sequence:

>777_bases
ATGTTGACCCGTCTCGGCGTCGCCGTGTTCTGGCTGCTGCACTGGCTGCCGCTGCCGCTGCTCGCGCGCATCGGCGAACT
GTTCGGCCTGCTGCTCTACCTGTTCGCCGTGCCGCGCCGGCGGGTGGTCGCCACCAACCTGAGGCTGTGTTTTCCGGAAT
TGTCCGACGGCGAACGCCGGCGCCTTGCCCGCGCCCATTTCCGCATCATCGGCCGCAGCCTGCTCGAACGCGGCCTGCTG
TGGTGGGCCAGCCCGGCGCGCCTGGAGCGCCTGGTGGCGGTCGAAGGGCTGGAGCGGCTGCGCGCGCTGCAGGCCGAAGG
CCGGCCGGTGCTGCTGCTGACGCCGCACTTCCTCGGGCTGGACATGGGCGGCACGCGGCTGTCGATGCTGGGCGACTTCG
TCAGCATCTACGCGCGGCAGAAGAACAAGGTGCTGGACCGCTGGCTCTACCACGGCCGCAGCCGCTTCGGCGACCAACTG
CTGCTGTCGCGCCAGGACGGCGTGCGCAGTACCGTGAAGGCGATGAAGGCAGGGCGGCCGTTCTACTCTACCTGCCGGAC
ATGGACTACGGCCGCAAGGACGCGATCTTCGTGCCCTTCTTCGGCGTGCCTGCCGCCACCATCACCGGGCTGCCGCGCTT
GGCGCGGCTGTCGGGCGCCGCCGTGCTGCCCTGCGTCACCCACATCCTGCCCGGTGGCGGCGGCTACAAGGTGGAGATCG
GCGAGCCCTGGCCGGACTTTCCCAGTGACGACGTGGAGGCCGATACCGCACGCATGA

Upstream 100 bases:

>100_bases
CCCGCCCAGTACCTGTGGGGCTACCACCGCTACAAGCGTCCCAAGGGCGCACGTGCCAAGGAGGCGCAATGAGCCCGCTC
CAATTCCGTCAGGGAACGCC

Downstream 100 bases:

>100_bases
ACGCGTGGATCGAGGCCAAGGTGCGGACGATGCCGGAGCAGTACTACTGGGTGCACCGCCGCTTCAAGACCCGTCCGCCG
GGAGAGGCGCGCTTCTACTG

Product: putative lipid A biosynthesis acyltransferase,truncated

Products: NA

Alternate protein names: Heat shock protein B [H]

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MLTRLGVAVFWLLHWLPLPLLARIGELFGLLLYLFAVPRRRVVATNLRLCFPELSDGERRRLARAHFRIIGRSLLERGLL
WWASPARLERLVAVEGLERLRALQAEGRPVLLLTPHFLGLDMGGTRLSMLGDFVSIYARQKNKVLDRWLYHGRSRFGDQL
LLSRQDGVRSTVKAMKAGRPFYSTCRTWTTAARTRSSCPSSACLPPPSPGCRAWRGCRAPPCCPASPTSCPVAAATRWRS
ASPGRTFPVTTWRPIPHA

Sequences:

>Translated_258_residues
MLTRLGVAVFWLLHWLPLPLLARIGELFGLLLYLFAVPRRRVVATNLRLCFPELSDGERRRLARAHFRIIGRSLLERGLL
WWASPARLERLVAVEGLERLRALQAEGRPVLLLTPHFLGLDMGGTRLSMLGDFVSIYARQKNKVLDRWLYHGRSRFGDQL
LLSRQDGVRSTVKAMKAGRPFYSTCRTWTTAARTRSSCPSSACLPPPSPGCRAWRGCRAPPCCPASPTSCPVAAATRWRS
ASPGRTFPVTTWRPIPHA
>Mature_258_residues
MLTRLGVAVFWLLHWLPLPLLARIGELFGLLLYLFAVPRRRVVATNLRLCFPELSDGERRRLARAHFRIIGRSLLERGLL
WWASPARLERLVAVEGLERLRALQAEGRPVLLLTPHFLGLDMGGTRLSMLGDFVSIYARQKNKVLDRWLYHGRSRFGDQL
LLSRQDGVRSTVKAMKAGRPFYSTCRTWTTAARTRSSCPSSACLPPPSPGCRAWRGCRAPPCCPASPTSCPVAAATRWRS
ASPGRTFPVTTWRPIPHA

Specific function: Acylates the intermediate (KDO)2-lipid IVA to form (KDO)2-(lauroyl)-lipid IVA. Has 10 fold selectivity for lauroyl- ACP over myristoyl-ACP [H]

COG id: COG1560

COG function: function code M; Lauroyl/myristoyl acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the htrB/msbB family [H]

Homologues:

Organism=Escherichia coli, GI1787292, Length=256, Percent_Identity=30.46875, Blast_Score=117, Evalue=8e-28,
Organism=Escherichia coli, GI87082094, Length=178, Percent_Identity=30.8988764044944, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1788161, Length=161, Percent_Identity=27.9503105590062, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004960
- InterPro:   IPR011920 [H]

Pfam domain/function: PF03279 Lip_A_acyltrans [H]

EC number: 2.3.1.-

Molecular weight: Translated: 29005; Mature: 29005

Theoretical pI: Translated: 11.98; Mature: 11.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTRLGVAVFWLLHWLPLPLLARIGELFGLLLYLFAVPRRRVVATNLRLCFPELSDGERR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHH
RLARAHFRIIGRSLLERGLLWWASPARLERLVAVEGLERLRALQAEGRPVLLLTPHFLGL
HHHHHHHHHHHHHHHHCCHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHCC
DMGGTRLSMLGDFVSIYARQKNKVLDRWLYHGRSRFGDQLLLSRQDGVRSTVKAMKAGRP
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCC
FYSTCRTWTTAARTRSSCPSSACLPPPSPGCRAWRGCRAPPCCPASPTSCPVAAATRWRS
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCHHHHHHCCC
ASPGRTFPVTTWRPIPHA
CCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MLTRLGVAVFWLLHWLPLPLLARIGELFGLLLYLFAVPRRRVVATNLRLCFPELSDGERR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHH
RLARAHFRIIGRSLLERGLLWWASPARLERLVAVEGLERLRALQAEGRPVLLLTPHFLGL
HHHHHHHHHHHHHHHHCCHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHCC
DMGGTRLSMLGDFVSIYARQKNKVLDRWLYHGRSRFGDQLLLSRQDGVRSTVKAMKAGRP
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCC
FYSTCRTWTTAARTRSSCPSSACLPPPSPGCRAWRGCRAPPCCPASPTSCPVAAATRWRS
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCHHHHHHCCC
ASPGRTFPVTTWRPIPHA
CCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Acyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]