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The map label for this gene is nifN [H]

Identifier: 119896852

GI number: 119896852

Start: 588618

End: 590012

Strand: Reverse

Name: nifN [H]

Synonym: azo0561

Alternate gene names: 119896852

Gene position: 590012-588618 (Counterclockwise)

Preceding gene: 119896853

Following gene: 119896851

Centisome position: 13.48

GC content: 69.1

Gene sequence:

>1395_bases
ATGGCCGAGATCATCCGTCACCCCAAGGCGCTGTCGGTCAGCCCGCTGAAATCCTCGGCGCCCGCCGGCGCCGCGCTAGC
CTTCCTCGGCATCAACCGCAGCATGCCGATCTTCCACGGCTCGCAGGGCTGCACCGCGTTCGCCAAGGTCTTCTTCGTGC
GCCATTTCCGCGAGCCGGTGCCGCTGCAGACGACCGCGATGGACCAGGTCGCCACCGTGATGAACGCCGACGGCAACGTG
GTGCAGGCGCTGGCCACCGTGTGCGGCAAGAGCAAGCCCTCGCTGGTCGGGCTGGCCACCACCGCGCTGTCCGAAACCCA
GGGCACCGACATCGCCCGCCTGGTCAAGGACTTCCGCGCCGACTTCCCGGAGTTCAACCACATCCCGGTCGCCGCGGTGA
ATACCCCGGATTTCGCCGGCTGCCTGGAAACCGGCTACGCCGCGGCGGTCAAGGGCATCCTCGATGCCACCGTGCCGCAC
ACCGCCGGCACCGGCATCGTCGGCAAGCGCAAGAAGCAGGTGAACCTGCTGCTCGGCAGCCACCTCACGCCGGGCGATGT
CGAAGCGATCAAGGAATGGCTGGAACTCTTCGGCCTGCGCCCGCTGTCGGTGCCCGACCTCGCCGATTCGCTCGACGGCC
ACCTGATCTCCGAGGACACCATCCCGGTCACGCTCGGCGGCACCCCGGTATCCGAACTCGCCACGCTGGGCGAAGCGGTC
GCCACCATCGTCGTCGGGCGCTCGCTGAAGGGCGCCGCCGACCTGCTCGCCGAACGCACCGGCGTGCCCGACTACCGCTT
CGACGGGCTGGTCGGACTGGAAGCCTGCGACCGCTTCACCCAGACGCTGGCCGAGATCGCCGGCGTCGCGGTGCCGGAAA
AGCTGGAACGCCATCGCGCGCAGCTGCAGGACGCGATGGTCGACACCCACTTCATGACCGGCCTGCGCCGCGTCGCCATC
GCCGCCGATCCCGACCTGCTGCTGCAGACCGCGACCCTGTTCGCCGGCATGGGCAGCGAGATCGTCGCCGCGGTGTCGCC
GATCAAGACCGGCAGCCTTACCGACGTACCGGGTGCGCCCCAGGTGCACGTGGGCGACCTCGAAGACCTCGAGGATCTGG
CCCGCACCGCACAGGCGGAACTCGTCGTCGCCAATTCACACGCCGCGGCCACGGCCGACCGCCTCGGCGTGCCCCTGCTG
CGGGCTGGCATGCCGCAGTACGACCTGGTCGGCGGTTACTGCAAGACGTGGGTGGGCTACCGGGGAACCCGGCAGGCCTT
GTTCGATATCGCCAACCTGGTGTTGCAACATCATGACTCCATCGCCCCACATCGATCCCGATTCAAGCTCGACCCCCAAC
CCGCGCCGGCGCCAGCTGCGGCTGGTGGGCACTGA

Upstream 100 bases:

>100_bases
CCGGACGCCGACGACGGCAACGACGCCCACGGTCATGATCACGACCACGATCACGACCACCACCACGCGCCCGCCGGCGC
TGCCGCGTAAGGAGCGCGCC

Downstream 100 bases:

>100_bases
GGAGGCCAAGCCGGTCATGGATGGCATTCGCGTCGCTTTCGCCAGCACCGACCGCAGCACGGTCAACCAGCACTTCGGCT
CCACCGAGGCCTTCGCCATC

Product: nitrogenase molybdenum-cofactor biosynthesis protein NifN

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 464; Mature: 463

Protein sequence:

>464_residues
MAEIIRHPKALSVSPLKSSAPAGAALAFLGINRSMPIFHGSQGCTAFAKVFFVRHFREPVPLQTTAMDQVATVMNADGNV
VQALATVCGKSKPSLVGLATTALSETQGTDIARLVKDFRADFPEFNHIPVAAVNTPDFAGCLETGYAAAVKGILDATVPH
TAGTGIVGKRKKQVNLLLGSHLTPGDVEAIKEWLELFGLRPLSVPDLADSLDGHLISEDTIPVTLGGTPVSELATLGEAV
ATIVVGRSLKGAADLLAERTGVPDYRFDGLVGLEACDRFTQTLAEIAGVAVPEKLERHRAQLQDAMVDTHFMTGLRRVAI
AADPDLLLQTATLFAGMGSEIVAAVSPIKTGSLTDVPGAPQVHVGDLEDLEDLARTAQAELVVANSHAAATADRLGVPLL
RAGMPQYDLVGGYCKTWVGYRGTRQALFDIANLVLQHHDSIAPHRSRFKLDPQPAPAPAAAGGH

Sequences:

>Translated_464_residues
MAEIIRHPKALSVSPLKSSAPAGAALAFLGINRSMPIFHGSQGCTAFAKVFFVRHFREPVPLQTTAMDQVATVMNADGNV
VQALATVCGKSKPSLVGLATTALSETQGTDIARLVKDFRADFPEFNHIPVAAVNTPDFAGCLETGYAAAVKGILDATVPH
TAGTGIVGKRKKQVNLLLGSHLTPGDVEAIKEWLELFGLRPLSVPDLADSLDGHLISEDTIPVTLGGTPVSELATLGEAV
ATIVVGRSLKGAADLLAERTGVPDYRFDGLVGLEACDRFTQTLAEIAGVAVPEKLERHRAQLQDAMVDTHFMTGLRRVAI
AADPDLLLQTATLFAGMGSEIVAAVSPIKTGSLTDVPGAPQVHVGDLEDLEDLARTAQAELVVANSHAAATADRLGVPLL
RAGMPQYDLVGGYCKTWVGYRGTRQALFDIANLVLQHHDSIAPHRSRFKLDPQPAPAPAAAGGH
>Mature_463_residues
AEIIRHPKALSVSPLKSSAPAGAALAFLGINRSMPIFHGSQGCTAFAKVFFVRHFREPVPLQTTAMDQVATVMNADGNVV
QALATVCGKSKPSLVGLATTALSETQGTDIARLVKDFRADFPEFNHIPVAAVNTPDFAGCLETGYAAAVKGILDATVPHT
AGTGIVGKRKKQVNLLLGSHLTPGDVEAIKEWLELFGLRPLSVPDLADSLDGHLISEDTIPVTLGGTPVSELATLGEAVA
TIVVGRSLKGAADLLAERTGVPDYRFDGLVGLEACDRFTQTLAEIAGVAVPEKLERHRAQLQDAMVDTHFMTGLRRVAIA
ADPDLLLQTATLFAGMGSEIVAAVSPIKTGSLTDVPGAPQVHVGDLEDLEDLARTAQAELVVANSHAAATADRLGVPLLR
AGMPQYDLVGGYCKTWVGYRGTRQALFDIANLVLQHHDSIAPHRSRFKLDPQPAPAPAAAGGH

Specific function: This protein may play a role in the biosynthesis of the prosthetic group of nitrogenase (FeMo cofactor) [H]

COG id: COG2710

COG function: function code C; Nitrogenase molybdenum-iron protein, alpha and beta chains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifD/nifK/nifE/nifN family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000510
- InterPro:   IPR000318
- InterPro:   IPR005975 [H]

Pfam domain/function: PF00148 Oxidored_nitro [H]

EC number: NA

Molecular weight: Translated: 48783; Mature: 48652

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: PS00699 NITROGENASE_1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEIIRHPKALSVSPLKSSAPAGAALAFLGINRSMPIFHGSQGCTAFAKVFFVRHFREPV
CCHHHCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCEECCCCHHHHHHHHHHHHHCCCC
PLQTTAMDQVATVMNADGNVVQALATVCGKSKPSLVGLATTALSETQGTDIARLVKDFRA
CCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEHHHHHHHHCCCCHHHHHHHHHHH
DFPEFNHIPVAAVNTPDFAGCLETGYAAAVKGILDATVPHTAGTGIVGKRKKQVNLLLGS
CCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCC
HLTPGDVEAIKEWLELFGLRPLSVPDLADSLDGHLISEDTIPVTLGGTPVSELATLGEAV
CCCCCHHHHHHHHHHHHCCCCCCCCHHHHHCCCCEECCCCEEEEECCCCHHHHHHHHHHH
ATIVVGRSLKGAADLLAERTGVPDYRFDGLVGLEACDRFTQTLAEIAGVAVPEKLERHRA
HHHHHCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
QLQDAMVDTHFMTGLRRVAIAADPDLLLQTATLFAGMGSEIVAAVSPIKTGSLTDVPGAP
HHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCC
QVHVGDLEDLEDLARTAQAELVVANSHAAATADRLGVPLLRAGMPQYDLVGGYCKTWVGY
CEECCCHHHHHHHHHHHHCEEEEECCCHHHHHHHHCCHHHHCCCCCHHHHHHHHHHHHCC
RGTRQALFDIANLVLQHHDSIAPHRSRFKLDPQPAPAPAAAGGH
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AEIIRHPKALSVSPLKSSAPAGAALAFLGINRSMPIFHGSQGCTAFAKVFFVRHFREPV
CHHHCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCEECCCCHHHHHHHHHHHHHCCCC
PLQTTAMDQVATVMNADGNVVQALATVCGKSKPSLVGLATTALSETQGTDIARLVKDFRA
CCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEHHHHHHHHCCCCHHHHHHHHHHH
DFPEFNHIPVAAVNTPDFAGCLETGYAAAVKGILDATVPHTAGTGIVGKRKKQVNLLLGS
CCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCC
HLTPGDVEAIKEWLELFGLRPLSVPDLADSLDGHLISEDTIPVTLGGTPVSELATLGEAV
CCCCCHHHHHHHHHHHHCCCCCCCCHHHHHCCCCEECCCCEEEEECCCCHHHHHHHHHHH
ATIVVGRSLKGAADLLAERTGVPDYRFDGLVGLEACDRFTQTLAEIAGVAVPEKLERHRA
HHHHHCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
QLQDAMVDTHFMTGLRRVAIAADPDLLLQTATLFAGMGSEIVAAVSPIKTGSLTDVPGAP
HHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCC
QVHVGDLEDLEDLARTAQAELVVANSHAAATADRLGVPLLRAGMPQYDLVGGYCKTWVGY
CEECCCHHHHHHHHHHHHCEEEEECCCHHHHHHHHCCHHHHCCCCCHHHHHHHHHHHHCC
RGTRQALFDIANLVLQHHDSIAPHRSRFKLDPQPAPAPAAAGGH
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2644218; 3470285 [H]