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The map label for this gene is nifE [H]

Identifier: 119896853

GI number: 119896853

Start: 590023

End: 591525

Strand: Reverse

Name: nifE [H]

Synonym: azo0562

Alternate gene names: 119896853

Gene position: 591525-590023 (Counterclockwise)

Preceding gene: 119896854

Following gene: 119896852

Centisome position: 13.52

GC content: 66.73

Gene sequence:

>1503_bases
GTGAAAGCCTCCGAAGTCGCTGCCCTGCTCGACGAACCCGCGTGCACCCACAACAAGAAGGAAAAATCCGGTTGCGCGCG
CCCCAAGCCCGGCGCCTCGGCCGGCGGTTGCGCCTTCGACGGCGCCCAGATCGCGCTGCTGCCGATTGCCGACGTCGCCC
ATGTGGTGCACGGCCCGATCGCCTGTGCGGGCAACTCCTGGGATAACCGCGGCACCAAGTCGTCCGGCCCCACGCTCTAC
AAGATCGGCATGACCACCGACCTTTCCGAGCAGGACATCATCATGGGCAAGGCGGAAAAGCGCCTGTTCCACGGCATCAA
GCAGGCGGTCGAGCGCTACAACCCGCCCGCGGTCTTCGTCTACAACACCTGTGTGCCGGCGCTGATCGGCGACGACATCG
AGGCGGTCTGCAAGGCCGCCGCCGAACGCTGGGGCGTGCCGGTGGTGCCGATCGACTCGGCCGGCTTCTACGGCACCAAG
AACCTCGGCAACCGCATCGCCGGCGAGGCCATGGTCAAGTACGTGATCGGCACCCGCGAGCCCGAGCCGCTGCCGCCGGA
AGCCATCCCGCCGGGCATCAAGGTGCACAACATCAACCTCATCGGCGAATACAACATCGCCGGCGAGCTGTGGTATCTCA
CCCCGCTGTTCGACGAACTCGGCATCCGCATCCTCTGTACGCTGTCGGGCGACGCCCGCTTCCACGAAGTGCAGACCATG
CACCGCGCCGAAGTGAACATGCTGGTGTGCGCCAAGGCGATGATCAACGTCGCCCGCAAGATGGAAGAGCGCTACGGCAT
CCCCTGGTTCGAGGGCAGCTTCTACGGCATCACCGACACCTCGGCGGCGCTGCGCGAGATCGCCCGCATCATCGGCGACG
AAGACCTGATCGCGCGCACCGAAGCCGTCATCGCCCGCGAGGAAAACCGCGTGCGCGGCGAACTCGACGTCTGGCGCCCG
CGCCTCGAAGGCAAGCGCGTGCTGCTCTTCACCGGCGGCGTGAAGTCGTGGTCGGTGGTGTCCGCGCTGCAGGACCTCGG
CATGAACGTGGTCGCCTCCGGCACCAAGAAATCCACCGAGGAAGACAAGGCCCGCATCCTCGAGCTGATGGGCGACAAGA
CCATCATGCTCAACAGCGAGGGCGCGCGCGACCTGCTCAACGCGGTGTACGAACTGAAGGCCGACATCCTCATCGCCGGC
GGCCGCAACCTCTACACCGCACTCAAGGCCCGCCTGCCCTTCCTCGACATCAACCAGGAACGCGAATTCGGCTACGCCGG
CTATGAGGGCATGAAGGAGCTGGTGCGCCAGCTCGCGCTCACCCTCGAATCGCCGGTGTGGGACGCGGTGCGCAAGCCGG
CGCCCTGGACCAGCAAGGCCATGGTGATCGCCGGGCCGGAACTGGGCGACAGCCCGGACGCCGACGACGGCAACGACGCC
CACGGTCATGATCACGACCACGATCACGACCACCACCACGCGCCCGCCGGCGCTGCCGCGTAA

Upstream 100 bases:

>100_bases
GAAGCAGAATTCGACCGTCGAATCAGCAACTTGCAATGCACGTCGGAAACGGCACGGTGCTTGCTGACCGAAGGACATCA
TTGCTGACGGAGTCCCCACC

Downstream 100 bases:

>100_bases
GGAGCGCGCCATGGCCGAGATCATCCGTCACCCCAAGGCGCTGTCGGTCAGCCCGCTGAAATCCTCGGCGCCCGCCGGCG
CCGCGCTAGCCTTCCTCGGC

Product: nitrogenase iron-molybdenum cofactor biosynthesis protein NifE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 500; Mature: 500

Protein sequence:

>500_residues
MKASEVAALLDEPACTHNKKEKSGCARPKPGASAGGCAFDGAQIALLPIADVAHVVHGPIACAGNSWDNRGTKSSGPTLY
KIGMTTDLSEQDIIMGKAEKRLFHGIKQAVERYNPPAVFVYNTCVPALIGDDIEAVCKAAAERWGVPVVPIDSAGFYGTK
NLGNRIAGEAMVKYVIGTREPEPLPPEAIPPGIKVHNINLIGEYNIAGELWYLTPLFDELGIRILCTLSGDARFHEVQTM
HRAEVNMLVCAKAMINVARKMEERYGIPWFEGSFYGITDTSAALREIARIIGDEDLIARTEAVIAREENRVRGELDVWRP
RLEGKRVLLFTGGVKSWSVVSALQDLGMNVVASGTKKSTEEDKARILELMGDKTIMLNSEGARDLLNAVYELKADILIAG
GRNLYTALKARLPFLDINQEREFGYAGYEGMKELVRQLALTLESPVWDAVRKPAPWTSKAMVIAGPELGDSPDADDGNDA
HGHDHDHDHDHHHAPAGAAA

Sequences:

>Translated_500_residues
MKASEVAALLDEPACTHNKKEKSGCARPKPGASAGGCAFDGAQIALLPIADVAHVVHGPIACAGNSWDNRGTKSSGPTLY
KIGMTTDLSEQDIIMGKAEKRLFHGIKQAVERYNPPAVFVYNTCVPALIGDDIEAVCKAAAERWGVPVVPIDSAGFYGTK
NLGNRIAGEAMVKYVIGTREPEPLPPEAIPPGIKVHNINLIGEYNIAGELWYLTPLFDELGIRILCTLSGDARFHEVQTM
HRAEVNMLVCAKAMINVARKMEERYGIPWFEGSFYGITDTSAALREIARIIGDEDLIARTEAVIAREENRVRGELDVWRP
RLEGKRVLLFTGGVKSWSVVSALQDLGMNVVASGTKKSTEEDKARILELMGDKTIMLNSEGARDLLNAVYELKADILIAG
GRNLYTALKARLPFLDINQEREFGYAGYEGMKELVRQLALTLESPVWDAVRKPAPWTSKAMVIAGPELGDSPDADDGNDA
HGHDHDHDHDHHHAPAGAAA
>Mature_500_residues
MKASEVAALLDEPACTHNKKEKSGCARPKPGASAGGCAFDGAQIALLPIADVAHVVHGPIACAGNSWDNRGTKSSGPTLY
KIGMTTDLSEQDIIMGKAEKRLFHGIKQAVERYNPPAVFVYNTCVPALIGDDIEAVCKAAAERWGVPVVPIDSAGFYGTK
NLGNRIAGEAMVKYVIGTREPEPLPPEAIPPGIKVHNINLIGEYNIAGELWYLTPLFDELGIRILCTLSGDARFHEVQTM
HRAEVNMLVCAKAMINVARKMEERYGIPWFEGSFYGITDTSAALREIARIIGDEDLIARTEAVIAREENRVRGELDVWRP
RLEGKRVLLFTGGVKSWSVVSALQDLGMNVVASGTKKSTEEDKARILELMGDKTIMLNSEGARDLLNAVYELKADILIAG
GRNLYTALKARLPFLDINQEREFGYAGYEGMKELVRQLALTLESPVWDAVRKPAPWTSKAMVIAGPELGDSPDADDGNDA
HGHDHDHDHDHHHAPAGAAA

Specific function: This protein may play a role in the biosynthesis of the prosthetic group of nitrogenase (FeMo cofactor) [H]

COG id: COG2710

COG function: function code C; Nitrogenase molybdenum-iron protein, alpha and beta chains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifD/nifK/nifE/nifN family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000510
- InterPro:   IPR000318
- InterPro:   IPR005973 [H]

Pfam domain/function: PF00148 Oxidored_nitro [H]

EC number: NA

Molecular weight: Translated: 54344; Mature: 54344

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: PS00699 NITROGENASE_1_1 ; PS00090 NITROGENASE_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKASEVAALLDEPACTHNKKEKSGCARPKPGASAGGCAFDGAQIALLPIADVAHVVHGPI
CCHHHHHHHHCCCCCCCCCHHHCCCCCCCCCCCCCCCEECCCCEEEEEHHHHHHHHHCCE
ACAGNSWDNRGTKSSGPTLYKIGMTTDLSEQDIIMGKAEKRLFHGIKQAVERYNPPAVFV
EECCCCCCCCCCCCCCCEEEEECCCCCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCEEEE
YNTCVPALIGDDIEAVCKAAAERWGVPVVPIDSAGFYGTKNLGNRIAGEAMVKYVIGTRE
ECCCHHHHHCCCHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCC
PEPLPPEAIPPGIKVHNINLIGEYNIAGELWYLTPLFDELGIRILCTLSGDARFHEVQTM
CCCCCCCCCCCCCEEEEEEEEEEECCCCEEEEECHHHHHCCCEEEEEECCCCHHHHHHHH
HRAEVNMLVCAKAMINVARKMEERYGIPWFEGSFYGITDTSAALREIARIIGDEDLIART
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCHHHHHHHHHHHHCCHHHHHHH
EAVIAREENRVRGELDVWRPRLEGKRVLLFTGGVKSWSVVSALQDLGMNVVASGTKKSTE
HHHHHHCCCCCCCCHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHCCCEEEECCCCCCCH
EDKARILELMGDKTIMLNSEGARDLLNAVYELKADILIAGGRNLYTALKARLPFLDINQE
HHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHCCCEEEECCCHHHHHHHHCCCEEECCCC
REFGYAGYEGMKELVRQLALTLESPVWDAVRKPAPWTSKAMVIAGPELGDSPDADDGNDA
CCCCCCCHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCC
HGHDHDHDHDHHHAPAGAAA
CCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKASEVAALLDEPACTHNKKEKSGCARPKPGASAGGCAFDGAQIALLPIADVAHVVHGPI
CCHHHHHHHHCCCCCCCCCHHHCCCCCCCCCCCCCCCEECCCCEEEEEHHHHHHHHHCCE
ACAGNSWDNRGTKSSGPTLYKIGMTTDLSEQDIIMGKAEKRLFHGIKQAVERYNPPAVFV
EECCCCCCCCCCCCCCCEEEEECCCCCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCEEEE
YNTCVPALIGDDIEAVCKAAAERWGVPVVPIDSAGFYGTKNLGNRIAGEAMVKYVIGTRE
ECCCHHHHHCCCHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCC
PEPLPPEAIPPGIKVHNINLIGEYNIAGELWYLTPLFDELGIRILCTLSGDARFHEVQTM
CCCCCCCCCCCCCEEEEEEEEEEECCCCEEEEECHHHHHCCCEEEEEECCCCHHHHHHHH
HRAEVNMLVCAKAMINVARKMEERYGIPWFEGSFYGITDTSAALREIARIIGDEDLIART
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCHHHHHHHHHHHHCCHHHHHHH
EAVIAREENRVRGELDVWRPRLEGKRVLLFTGGVKSWSVVSALQDLGMNVVASGTKKSTE
HHHHHHCCCCCCCCHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHCCCEEEECCCCCCCH
EDKARILELMGDKTIMLNSEGARDLLNAVYELKADILIAGGRNLYTALKARLPFLDINQE
HHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHCCCEEEECCCHHHHHHHHCCCEEECCCC
REFGYAGYEGMKELVRQLALTLESPVWDAVRKPAPWTSKAMVIAGPELGDSPDADDGNDA
CCCCCCCHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCC
HGHDHDHDHDHHHAPAGAAA
CCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2644218; 3387235; 3470285 [H]