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The map label for this gene is nifU [H]

Identifier: 119896844

GI number: 119896844

Start: 584713

End: 585603

Strand: Reverse

Name: nifU [H]

Synonym: azo0553

Alternate gene names: 119896844

Gene position: 585603-584713 (Counterclockwise)

Preceding gene: 119896845

Following gene: 119896843

Centisome position: 13.38

GC content: 65.1

Gene sequence:

>891_bases
ATGTGGGATTACTCCGATACCGTCAAGGAACACTTCTACAACCCCCGCAACGCCGGCGCGCTGGCCGACGCCAACGCCAT
CGGCGAAGTGGGTTCGATCTCCTGCGGTGACGCGCTGCGCCTGATGCTCAAGGTCGACCCGGCCAACGAGACCATCCTCG
ACGCCTCCTTCCAGACCTTCGGCTGCGGCTCGGCGATCGCCTCCAGCTCAGCGCTGACCGAGATCATCAAGGGCAAGACG
CTCGAGCAGGCGCTCGAAGTCTCCAACCAGGACATCGCCGACTTCCTCGGCGGCCTGCCGCCGGAAAAGATGCACTGCTC
GGTGATGGGCCGCGAAGCCCTGCAGGCCGCGGTCGCCAACTACCGCGGCGAAGAATGGAAGGACGACCACGAAGAAGGCG
CGCTGATCTGCAAGTGCTTCGCGGTCGACGCGGTGATGATCGAAGAGACCATCCGCGCCAACAACCTGTGCTCGGTCGAG
CAGGTCACCAACTACACCAAGGCTGGCGGCGGCTGTTCCTCCTGCCACGAAGGCATCGAGGAAATCCTCGCCAAGGTGCT
GGCCGAGCGTGGCGAAACCTTCAAGGCCGCCCCGCCGCCGGAAAAGAAGGCGCCGGGCAAGATGACGACGCTGGAGCGCA
TCCGCAAGATCGAGCAGGTCATCGAAAAGGCCCGCCCCAACCTGCAGCGCGACCACGGCGACATCGAACTGGTCGACGTC
GACGGCAAGAACATCTACGTGTCGATGAAGGGCGCCTGCGCCGGCTGCCAGATGGAAGCGGCCACCCTCGGCGGCGTCCA
GGCCCAGATGATCGAAGCGCTCGGCGAACTGGTGAAGGTGATCCCGGTGAAGGCCACCGCCAAGCTGGCGCCCAACACCC
TGCCCGCCTGA

Upstream 100 bases:

>100_bases
GCTGACCGGCTCCGGCTTCAAGTTCGAAAACCCCAACGCCACCGGCGGCTGCGCCTGTGGCCAGTCCTTCTCCGCCTAAG
CCCAGTACCTGGAGACAGCC

Downstream 100 bases:

>100_bases
TCCGCCCTGCCTGCCCCACCACCCGGCGGGGCAGGCCGCATACCCGCTGCGCGGCGGGTGTCCGCCGCGCAGCCGCAGCA
CCGACATTCACGAGATAAAC

Product: nitrogen fixation protein NifU

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MWDYSDTVKEHFYNPRNAGALADANAIGEVGSISCGDALRLMLKVDPANETILDASFQTFGCGSAIASSSALTEIIKGKT
LEQALEVSNQDIADFLGGLPPEKMHCSVMGREALQAAVANYRGEEWKDDHEEGALICKCFAVDAVMIEETIRANNLCSVE
QVTNYTKAGGGCSSCHEGIEEILAKVLAERGETFKAAPPPEKKAPGKMTTLERIRKIEQVIEKARPNLQRDHGDIELVDV
DGKNIYVSMKGACAGCQMEAATLGGVQAQMIEALGELVKVIPVKATAKLAPNTLPA

Sequences:

>Translated_296_residues
MWDYSDTVKEHFYNPRNAGALADANAIGEVGSISCGDALRLMLKVDPANETILDASFQTFGCGSAIASSSALTEIIKGKT
LEQALEVSNQDIADFLGGLPPEKMHCSVMGREALQAAVANYRGEEWKDDHEEGALICKCFAVDAVMIEETIRANNLCSVE
QVTNYTKAGGGCSSCHEGIEEILAKVLAERGETFKAAPPPEKKAPGKMTTLERIRKIEQVIEKARPNLQRDHGDIELVDV
DGKNIYVSMKGACAGCQMEAATLGGVQAQMIEALGELVKVIPVKATAKLAPNTLPA
>Mature_296_residues
MWDYSDTVKEHFYNPRNAGALADANAIGEVGSISCGDALRLMLKVDPANETILDASFQTFGCGSAIASSSALTEIIKGKT
LEQALEVSNQDIADFLGGLPPEKMHCSVMGREALQAAVANYRGEEWKDDHEEGALICKCFAVDAVMIEETIRANNLCSVE
QVTNYTKAGGGCSSCHEGIEEILAKVLAERGETFKAAPPPEKKAPGKMTTLERIRKIEQVIEKARPNLQRDHGDIELVDV
DGKNIYVSMKGACAGCQMEAATLGGVQAQMIEALGELVKVIPVKATAKLAPNTLPA

Specific function: Involved in the formation or repair of [Fe-S] clusters present in iron-sulfur proteins [H]

COG id: COG0822

COG function: function code C; NifU homolog involved in Fe-S cluster formation

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifU family [H]

Homologues:

Organism=Homo sapiens, GI24307953, Length=123, Percent_Identity=49.5934959349593, Blast_Score=119, Evalue=3e-27,
Organism=Homo sapiens, GI56699456, Length=122, Percent_Identity=49.1803278688525, Blast_Score=118, Evalue=7e-27,
Organism=Escherichia coli, GI1788878, Length=125, Percent_Identity=46.4, Blast_Score=114, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI17543474, Length=128, Percent_Identity=46.875, Blast_Score=113, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6325122, Length=125, Percent_Identity=44, Blast_Score=113, Evalue=4e-26,
Organism=Saccharomyces cerevisiae, GI6324800, Length=125, Percent_Identity=40.8, Blast_Score=109, Evalue=6e-25,
Organism=Drosophila melanogaster, GI21355597, Length=127, Percent_Identity=48.0314960629921, Blast_Score=119, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007419
- InterPro:   IPR016217
- InterPro:   IPR010238
- InterPro:   IPR001075
- InterPro:   IPR002871
- ProDom:   PD002830 [H]

Pfam domain/function: PF04324 Fer2_BFD; PF01106 NifU; PF01592 NifU_N [H]

EC number: NA

Molecular weight: Translated: 31668; Mature: 31668

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWDYSDTVKEHFYNPRNAGALADANAIGEVGSISCGDALRLMLKVDPANETILDASFQTF
CCCCHHHHHHHCCCCCCCCCCCCCHHHCCCCCCCCCHHEEEEEEECCCCCEEECCCCHHC
GCGSAIASSSALTEIIKGKTLEQALEVSNQDIADFLGGLPPEKMHCSVMGREALQAAVAN
CCHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
YRGEEWKDDHEEGALICKCFAVDAVMIEETIRANNLCSVEQVTNYTKAGGGCSSCHEGIE
CCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHH
EILAKVLAERGETFKAAPPPEKKAPGKMTTLERIRKIEQVIEKARPNLQRDHGDIELVDV
HHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEE
DGKNIYVSMKGACAGCQMEAATLGGVQAQMIEALGELVKVIPVKATAKLAPNTLPA
CCCEEEEEECCCCCCCEEHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCC
>Mature Secondary Structure
MWDYSDTVKEHFYNPRNAGALADANAIGEVGSISCGDALRLMLKVDPANETILDASFQTF
CCCCHHHHHHHCCCCCCCCCCCCCHHHCCCCCCCCCHHEEEEEEECCCCCEEECCCCHHC
GCGSAIASSSALTEIIKGKTLEQALEVSNQDIADFLGGLPPEKMHCSVMGREALQAAVAN
CCHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
YRGEEWKDDHEEGALICKCFAVDAVMIEETIRANNLCSVEQVTNYTKAGGGCSSCHEGIE
CCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHH
EILAKVLAERGETFKAAPPPEKKAPGKMTTLERIRKIEQVIEKARPNLQRDHGDIELVDV
HHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEE
DGKNIYVSMKGACAGCQMEAATLGGVQAQMIEALGELVKVIPVKATAKLAPNTLPA
CCCEEEEEECCCCCCCEEHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2644218; 3040672; 7947754; 10819462 [H]