Click here to switch to the map view.

The map label for this gene is nifS [H]

Identifier: 119896843

GI number: 119896843

Start: 583307

End: 584530

Strand: Reverse

Name: nifS [H]

Synonym: azo0552

Alternate gene names: 119896843

Gene position: 584530-583307 (Counterclockwise)

Preceding gene: 119896844

Following gene: 119896842

Centisome position: 13.36

GC content: 66.91

Gene sequence:

>1224_bases
ATGAGCATCCCGAACCTGACCCCGATCTATCTCGACAACAACGCCACCACCCGCTGCGCCCCCGAAGCGGTGACGGCGAT
GCTGCCCTACTTCACCGAACAGTTCGGCAACCCGTCCTCGATGCACAGCTTCGGCGCCAAGGTCGGCCATGCCACCAAGG
GCGCGCGTGCCTCGCTGCAGAAGCTGCTCGGCGCCGAGTTCGATTCCGAGATCATCTACACCGCCGGCGGCACCGAGGCC
GACAACACCGCCATCCTGTCGGCGCTCAAGGCCAACCCGGAGCGCAAGGAAATCATCACCAGCACCGTGGAGCACCACGC
CGTGCTGCACCTGTGCGAACAGCTCGAAAAAGAGGGCTACACCATCCACAAGCTCAAGGTGGACGGCAAGGGCCGGCTGG
ACCTCGACGAATACGCCAAGCTGCTGACCGACAAGGTCGCGGTGGTGTCGGTGATGTGGGCCAACAACGAAACCGGCACC
TACTTCCCGGTCGAGAAGATGGCCGAGATGGCCAAGGCCGCCGGCATCCTGTTCCACACCGACGCGGTCCAGGCGGTCGG
CAAGATTCCGCTCAACCTCAAGGATTCGGCCATCGACATGCTGTCGCTGTCGGGCCACAAGCTGCACGGGCCGAAGGGCA
TCGGCGCGCTCTACCTGCGCCGCGGCGCCCGCTTCCGCCCGCTGCTGCGCGGCGGCGGCCAGGAACGCGGCCGCCGTGCC
GGTACCGAGAACGTGCCCGGCATCATCGGCCTTGGCGTCGCCGCCGAACTGGCGCTGGAGCACATGGAGCAGGAGAACAC
CTACGTCCGCGCGCTGCGCGACAAGCTGCAGGCCGGCATCCTCGCCGCCGTGCCCAATGCCTTCGCCACCGGCGACGTCG
ACAACCGCCTGCCGAACACGCTCAACATCGCCTTCGAATACGTCGAAGGCGAGGCCATCCTGATGCTGCTGAACAAGCAG
GGCATCGCTGCATCGTCCGGCTCGGCCTGCACCTCCGGCTCGCTCGAACCCTCGCACGTGATGCGCGCCATGGGCATCCC
CTACACCGCCGCCCACGGCACCATCCGCTTCTCGCTGTCGCGCGAGAACACCGAGGCCGAGATCGACCGCGTGATCGCCG
CGGTGCCGCCGATCATCGCCCAGCTGCGCAAGCTGTCGCCGTACTGGGGCGAGAACGGCCCGGTCACCGATCCGGAAAAG
GCCTTCGCCCCGACCTACGCCTAA

Upstream 100 bases:

>100_bases
GACATTCACGAGATAAACCACCGCCACCGCCCCTGCAGCGCGCCGCCGGCCAACCCGGCAGCCCCAGTCCGGCGAACACG
GTAGCCCCGCGGAGAGCACG

Downstream 100 bases:

>100_bases
GCCGCGCGCGCCGTCGCCACCCGCGACGGCGCCGCACCGGACCCGCCGCCTCCACCCGACGCGAACGACACCAGCCCAGG
CCACGAGCATGTCTGCACCA

Product: cysteine desulphurase

Products: NA

Alternate protein names: Nitrogenase metalloclusters biosynthesis protein NifS [H]

Number of amino acids: Translated: 407; Mature: 406

Protein sequence:

>407_residues
MSIPNLTPIYLDNNATTRCAPEAVTAMLPYFTEQFGNPSSMHSFGAKVGHATKGARASLQKLLGAEFDSEIIYTAGGTEA
DNTAILSALKANPERKEIITSTVEHHAVLHLCEQLEKEGYTIHKLKVDGKGRLDLDEYAKLLTDKVAVVSVMWANNETGT
YFPVEKMAEMAKAAGILFHTDAVQAVGKIPLNLKDSAIDMLSLSGHKLHGPKGIGALYLRRGARFRPLLRGGGQERGRRA
GTENVPGIIGLGVAAELALEHMEQENTYVRALRDKLQAGILAAVPNAFATGDVDNRLPNTLNIAFEYVEGEAILMLLNKQ
GIAASSGSACTSGSLEPSHVMRAMGIPYTAAHGTIRFSLSRENTEAEIDRVIAAVPPIIAQLRKLSPYWGENGPVTDPEK
AFAPTYA

Sequences:

>Translated_407_residues
MSIPNLTPIYLDNNATTRCAPEAVTAMLPYFTEQFGNPSSMHSFGAKVGHATKGARASLQKLLGAEFDSEIIYTAGGTEA
DNTAILSALKANPERKEIITSTVEHHAVLHLCEQLEKEGYTIHKLKVDGKGRLDLDEYAKLLTDKVAVVSVMWANNETGT
YFPVEKMAEMAKAAGILFHTDAVQAVGKIPLNLKDSAIDMLSLSGHKLHGPKGIGALYLRRGARFRPLLRGGGQERGRRA
GTENVPGIIGLGVAAELALEHMEQENTYVRALRDKLQAGILAAVPNAFATGDVDNRLPNTLNIAFEYVEGEAILMLLNKQ
GIAASSGSACTSGSLEPSHVMRAMGIPYTAAHGTIRFSLSRENTEAEIDRVIAAVPPIIAQLRKLSPYWGENGPVTDPEK
AFAPTYA
>Mature_406_residues
SIPNLTPIYLDNNATTRCAPEAVTAMLPYFTEQFGNPSSMHSFGAKVGHATKGARASLQKLLGAEFDSEIIYTAGGTEAD
NTAILSALKANPERKEIITSTVEHHAVLHLCEQLEKEGYTIHKLKVDGKGRLDLDEYAKLLTDKVAVVSVMWANNETGTY
FPVEKMAEMAKAAGILFHTDAVQAVGKIPLNLKDSAIDMLSLSGHKLHGPKGIGALYLRRGARFRPLLRGGGQERGRRAG
TENVPGIIGLGVAAELALEHMEQENTYVRALRDKLQAGILAAVPNAFATGDVDNRLPNTLNIAFEYVEGEAILMLLNKQG
IAASSGSACTSGSLEPSHVMRAMGIPYTAAHGTIRFSLSRENTEAEIDRVIAAVPPIIAQLRKLSPYWGENGPVTDPEKA
FAPTYA

Specific function: Catalyzes the removal of elemental sulfur atoms from cysteine to produce alanine. Seems to participate in the biosynthesis of the nitrogenase metalloclusters by providing the inorganic sulfur required for the Fe-S core formation [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=390, Percent_Identity=42.5641025641026, Blast_Score=320, Evalue=1e-87,
Organism=Homo sapiens, GI156713448, Length=416, Percent_Identity=36.5384615384615, Blast_Score=214, Evalue=1e-55,
Organism=Escherichia coli, GI48994898, Length=388, Percent_Identity=45.360824742268, Blast_Score=319, Evalue=2e-88,
Organism=Escherichia coli, GI1789175, Length=396, Percent_Identity=28.5353535353535, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1787970, Length=396, Percent_Identity=26.010101010101, Blast_Score=94, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI25143064, Length=387, Percent_Identity=42.6356589147287, Blast_Score=309, Evalue=2e-84,
Organism=Caenorhabditis elegans, GI17533177, Length=312, Percent_Identity=31.7307692307692, Blast_Score=160, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6319831, Length=390, Percent_Identity=43.5897435897436, Blast_Score=307, Evalue=2e-84,
Organism=Drosophila melanogaster, GI20129463, Length=386, Percent_Identity=42.4870466321244, Blast_Score=316, Evalue=2e-86,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR017772
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 43772; Mature: 43640

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIPNLTPIYLDNNATTRCAPEAVTAMLPYFTEQFGNPSSMHSFGAKVGHATKGARASLQ
CCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHH
KLLGAEFDSEIIYTAGGTEADNTAILSALKANPERKEIITSTVEHHAVLHLCEQLEKEGY
HHHCCCCCCCEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
TIHKLKVDGKGRLDLDEYAKLLTDKVAVVSVMWANNETGTYFPVEKMAEMAKAAGILFHT
EEEEEEECCCCCCCHHHHHHHHHHHEEEEEEEEECCCCCCEECHHHHHHHHHHCCEEEEH
DAVQAVGKIPLNLKDSAIDMLSLSGHKLHGPKGIGALYLRRGARFRPLLRGGGQERGRRA
HHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCCHHHHCCCHHHHHCC
GTENVPGIIGLGVAAELALEHMEQENTYVRALRDKLQAGILAAVPNAFATGDVDNRLPNT
CCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCCCCCCCCCCE
LNIAFEYVEGEAILMLLNKQGIAASSGSACTSGSLEPSHVMRAMGIPYTAAHGTIRFSLS
EEEEEEEECCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHCCCEEECCCEEEEEEC
RENTEAEIDRVIAAVPPIIAQLRKLSPYWGENGPVTDPEKAFAPTYA
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHCCCCCCC
>Mature Secondary Structure 
SIPNLTPIYLDNNATTRCAPEAVTAMLPYFTEQFGNPSSMHSFGAKVGHATKGARASLQ
CCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHH
KLLGAEFDSEIIYTAGGTEADNTAILSALKANPERKEIITSTVEHHAVLHLCEQLEKEGY
HHHCCCCCCCEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
TIHKLKVDGKGRLDLDEYAKLLTDKVAVVSVMWANNETGTYFPVEKMAEMAKAAGILFHT
EEEEEEECCCCCCCHHHHHHHHHHHEEEEEEEEECCCCCCEECHHHHHHHHHHCCEEEEH
DAVQAVGKIPLNLKDSAIDMLSLSGHKLHGPKGIGALYLRRGARFRPLLRGGGQERGRRA
HHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCCHHHHCCCHHHHHCC
GTENVPGIIGLGVAAELALEHMEQENTYVRALRDKLQAGILAAVPNAFATGDVDNRLPNT
CCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCCCCCCCCCCE
LNIAFEYVEGEAILMLLNKQGIAASSGSACTSGSLEPSHVMRAMGIPYTAAHGTIRFSLS
EEEEEEEECCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHCCCEEECCCEEEEEEC
RENTEAEIDRVIAAVPPIIAQLRKLSPYWGENGPVTDPEKAFAPTYA
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2644218; 3040672; 8464885; 8161529 [H]