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The map label for this gene is nifK [H]

Identifier: 119896831

GI number: 119896831

Start: 573809

End: 575377

Strand: Direct

Name: nifK [H]

Synonym: azo0540

Alternate gene names: 119896831

Gene position: 573809-575377 (Clockwise)

Preceding gene: 119896830

Following gene: 119896832

Centisome position: 13.11

GC content: 60.68

Gene sequence:

>1569_bases
ATGCAAGCCGTAGACGATATCAAGCCGTGTTACCCGCTGTTCCGTGACGACGACTACAAGGAAAACCTGAAGCGCAAGCA
GGACCTGTACGAAGAGCGCGTTCCGGCCGACAAGATCGCCGAAACCTTCGAATGGACCACCTCGAAGGAATACCAGGAAC
TGAACTTCAAGCGCGAAGCGATCACCATCAACCCCGCCAAGGCCTGCCAGCCGCTGGGCGCGGTGCTGTGCTCGCTGGGC
TTCAAGAACACGATGCCCTACGTGCATGGTTCGCAGGGTTGCGTGGCCTACTTCCGCACCTACTTCAACCGTCACTTCAA
GGAGCCGGTGTCCTGCGTGTCCGACTCCATGACGGAAGATGCGGCGGTGTTCGGCGGCCAGAAGAACATGTTCGACGGCC
TGGAAAACGCCAAGGCGATCTACAAGCCGGACATGATCGCGGTCTCCACCACCTGCATGGCCGAGGTGATCGGTGACGAC
TTGAACGCCTTCATCAACAACGCGAAGAAGGCCGGTCACGTTCCGCAGGAATACCCGGTTCCGTTCGCCCACACCCCGTC
CTTCGTCGGCAGCCACACCACCGGCTGGGACAACATGTGGGAAGGCATCGCGCGCTTCTTCACGCTGAACAAGATGGAAG
GCAAGGTTGTCGGCAGCAACGGCAAGCTGAACTTCGTGCCGGGCTTCGAAACCTACCTGGGCAACTACCGCGTCATCAAG
CGCATGATGGGTCAGATGGGTGTCGACGCCACCGTGCTGTCCGATCCGAGCGAAGTGCTGGATACCCCGTCCGACGGCGA
ATTCCGCATGTACTCGGGCGGCACCACGATGGACGAGATGTCCGATGCGCCGAACGCGCTCAACACCATCGTGCTGCAGC
CGTGGCAACTCGAGAAGTCCAAGAAGTTCGTGGAAACCGTGTGGAACCACGACGTGCCCAAGCTCAACATCCCGATGGGC
CTGGACTGGACCGACGAGTTCCTGATGAAGGTCTCCGAGATCACCGGCAAGGCGATTCCGGAAGAGCTGGCGCTCGAGCG
TGGCCGCCTGGTCGACATGATGCAGGACTCCCACACCTGGCTGCACGGCAAGAAGTTCGCCCTGTACGGCGACCCGGACT
TCGTGCTCGGCCTGACCAAGATCCTGCTCGAACTCGGCGCGGAACCGATCCACATCCTGTCGAACAACGCCAACAAGCGT
TGGGGCAAGGCGGTGCAGAAGGTGCTCGACGGCTACAAGCACGGCGCCAACTGCAAGGTGTATGTCGGCAACGACCTGTG
GCACATGCGCTCGCTGTGCTTCACCGAGAAGCCGGACTTCCTGATCGGCAACTCGTACGGCAAGTACATCCAGCGTGACA
CGGTGTTCAAGGGCAAGGAATTCGAGGTTCCGCTGATCCGCCTCGGCTTCCCGATCTTCGACCGCCACCACCTGCACCGC
ATGACCACCCTGGGCTACGAAGGCGCGATGTATGTGCTGACCACCCTGGTGAACGCCGTCCTCGAGCGTCTGGACGACGA
AACCCGCGGCATGGGTACCACCGACTACAACTACGACCTCGTTCGCTAA

Upstream 100 bases:

>100_bases
ACCTTCCGGATGGACCTGGTGCGCCGGGGTCGGCGCACCAGGTATCGAATGCACTCAAGCCCGTGTCCACAGATTGCTGG
CCGGGTTAAGGAGCTGAAAA

Downstream 100 bases:

>100_bases
TCAGCGATCACGGGACGGGCGCCTTCGCGGCACCCGTCCCGCTTTCACGTTTGCGAACCCTGCATGCGGGCGTCATCGTG
TCCGCGCGCGTAATTGGAGA

Product: nitrogenase molybdenum-iron protein subunit beta

Products: NA

Alternate protein names: Dinitrogenase; Nitrogenase component I [H]

Number of amino acids: Translated: 522; Mature: 522

Protein sequence:

>522_residues
MQAVDDIKPCYPLFRDDDYKENLKRKQDLYEERVPADKIAETFEWTTSKEYQELNFKREAITINPAKACQPLGAVLCSLG
FKNTMPYVHGSQGCVAYFRTYFNRHFKEPVSCVSDSMTEDAAVFGGQKNMFDGLENAKAIYKPDMIAVSTTCMAEVIGDD
LNAFINNAKKAGHVPQEYPVPFAHTPSFVGSHTTGWDNMWEGIARFFTLNKMEGKVVGSNGKLNFVPGFETYLGNYRVIK
RMMGQMGVDATVLSDPSEVLDTPSDGEFRMYSGGTTMDEMSDAPNALNTIVLQPWQLEKSKKFVETVWNHDVPKLNIPMG
LDWTDEFLMKVSEITGKAIPEELALERGRLVDMMQDSHTWLHGKKFALYGDPDFVLGLTKILLELGAEPIHILSNNANKR
WGKAVQKVLDGYKHGANCKVYVGNDLWHMRSLCFTEKPDFLIGNSYGKYIQRDTVFKGKEFEVPLIRLGFPIFDRHHLHR
MTTLGYEGAMYVLTTLVNAVLERLDDETRGMGTTDYNYDLVR

Sequences:

>Translated_522_residues
MQAVDDIKPCYPLFRDDDYKENLKRKQDLYEERVPADKIAETFEWTTSKEYQELNFKREAITINPAKACQPLGAVLCSLG
FKNTMPYVHGSQGCVAYFRTYFNRHFKEPVSCVSDSMTEDAAVFGGQKNMFDGLENAKAIYKPDMIAVSTTCMAEVIGDD
LNAFINNAKKAGHVPQEYPVPFAHTPSFVGSHTTGWDNMWEGIARFFTLNKMEGKVVGSNGKLNFVPGFETYLGNYRVIK
RMMGQMGVDATVLSDPSEVLDTPSDGEFRMYSGGTTMDEMSDAPNALNTIVLQPWQLEKSKKFVETVWNHDVPKLNIPMG
LDWTDEFLMKVSEITGKAIPEELALERGRLVDMMQDSHTWLHGKKFALYGDPDFVLGLTKILLELGAEPIHILSNNANKR
WGKAVQKVLDGYKHGANCKVYVGNDLWHMRSLCFTEKPDFLIGNSYGKYIQRDTVFKGKEFEVPLIRLGFPIFDRHHLHR
MTTLGYEGAMYVLTTLVNAVLERLDDETRGMGTTDYNYDLVR
>Mature_522_residues
MQAVDDIKPCYPLFRDDDYKENLKRKQDLYEERVPADKIAETFEWTTSKEYQELNFKREAITINPAKACQPLGAVLCSLG
FKNTMPYVHGSQGCVAYFRTYFNRHFKEPVSCVSDSMTEDAAVFGGQKNMFDGLENAKAIYKPDMIAVSTTCMAEVIGDD
LNAFINNAKKAGHVPQEYPVPFAHTPSFVGSHTTGWDNMWEGIARFFTLNKMEGKVVGSNGKLNFVPGFETYLGNYRVIK
RMMGQMGVDATVLSDPSEVLDTPSDGEFRMYSGGTTMDEMSDAPNALNTIVLQPWQLEKSKKFVETVWNHDVPKLNIPMG
LDWTDEFLMKVSEITGKAIPEELALERGRLVDMMQDSHTWLHGKKFALYGDPDFVLGLTKILLELGAEPIHILSNNANKR
WGKAVQKVLDGYKHGANCKVYVGNDLWHMRSLCFTEKPDFLIGNSYGKYIQRDTVFKGKEFEVPLIRLGFPIFDRHHLHR
MTTLGYEGAMYVLTTLVNAVLERLDDETRGMGTTDYNYDLVR

Specific function: This molybdenum-iron protein is part of the nitrogenase complex that catalyzes the key enzymatic reactions in nitrogen fixation [H]

COG id: COG2710

COG function: function code C; Nitrogenase molybdenum-iron protein, alpha and beta chains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifD/nifK/nifE/nifN family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000510
- InterPro:   IPR000318
- InterPro:   IPR005976 [H]

Pfam domain/function: PF00148 Oxidored_nitro [H]

EC number: =1.18.6.1 [H]

Molecular weight: Translated: 59187; Mature: 59187

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: PS00699 NITROGENASE_1_1 ; PS00090 NITROGENASE_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQAVDDIKPCYPLFRDDDYKENLKRKQDLYEERVPADKIAETFEWTTSKEYQELNFKREA
CCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHCCCCEE
ITINPAKACQPLGAVLCSLGFKNTMPYVHGSQGCVAYFRTYFNRHFKEPVSCVSDSMTED
EEECHHHHHHHHHHHHHHCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCHH
AAVFGGQKNMFDGLENAKAIYKPDMIAVSTTCMAEVIGDDLNAFINNAKKAGHVPQEYPV
HHHHCCCHHHHHHHHHCHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
PFAHTPSFVGSHTTGWDNMWEGIARFFTLNKMEGKVVGSNGKLNFVPGFETYLGNYRVIK
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEECCCCCEEECCCHHHHHCCHHHHH
RMMGQMGVDATVLSDPSEVLDTPSDGEFRMYSGGTTMDEMSDAPNALNTIVLQPWQLEKS
HHHHHCCCCEEEECCCHHHHCCCCCCCEEEEECCCCHHHHHCCCCHHHHEEECCCCCHHH
KKFVETVWNHDVPKLNIPMGLDWTDEFLMKVSEITGKAIPEELALERGRLVDMMQDSHTW
HHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHCCCCE
LHGKKFALYGDPDFVLGLTKILLELGAEPIHILSNNANKRWGKAVQKVLDGYKHGANCKV
ECCCEEEEECCCHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCEEE
YVGNDLWHMRSLCFTEKPDFLIGNSYGKYIQRDTVFKGKEFEVPLIRLGFPIFDRHHLHR
EECCCHHHHHHHHHCCCCCEEEECCHHHHHHHCCCCCCCCCCCCHHEECCCHHHHHHHHH
MTTLGYEGAMYVLTTLVNAVLERLDDETRGMGTTDYNYDLVR
HHHCCCCCHHHHHHHHHHHHHHHHCHHHCCCCCCCCCCEECC
>Mature Secondary Structure
MQAVDDIKPCYPLFRDDDYKENLKRKQDLYEERVPADKIAETFEWTTSKEYQELNFKREA
CCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHCCCCEE
ITINPAKACQPLGAVLCSLGFKNTMPYVHGSQGCVAYFRTYFNRHFKEPVSCVSDSMTED
EEECHHHHHHHHHHHHHHCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCHH
AAVFGGQKNMFDGLENAKAIYKPDMIAVSTTCMAEVIGDDLNAFINNAKKAGHVPQEYPV
HHHHCCCHHHHHHHHHCHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
PFAHTPSFVGSHTTGWDNMWEGIARFFTLNKMEGKVVGSNGKLNFVPGFETYLGNYRVIK
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEECCCCCEEECCCHHHHHCCHHHHH
RMMGQMGVDATVLSDPSEVLDTPSDGEFRMYSGGTTMDEMSDAPNALNTIVLQPWQLEKS
HHHHHCCCCEEEECCCHHHHCCCCCCCEEEEECCCCHHHHHCCCCHHHHEEECCCCCHHH
KKFVETVWNHDVPKLNIPMGLDWTDEFLMKVSEITGKAIPEELALERGRLVDMMQDSHTW
HHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHCCCCE
LHGKKFALYGDPDFVLGLTKILLELGAEPIHILSNNANKRWGKAVQKVLDGYKHGANCKV
ECCCEEEEECCCHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCEEE
YVGNDLWHMRSLCFTEKPDFLIGNSYGKYIQRDTVFKGKEFEVPLIRLGFPIFDRHHLHR
EECCCHHHHHHHHHCCCCCEEEECCHHHHHHHCCCCCCCCCCCCHHEECCCHHHHHHHHH
MTTLGYEGAMYVLTTLVNAVLERLDDETRGMGTTDYNYDLVR
HHHCCCCCHHHHHHHHHHHHHHHHCHHHCCCCCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2644218; 3863780; 649581; 9163420; 9063865; 11170380; 11951047; 12215645 [H]