Click here to switch to the map view.

The map label for this gene is nifD [H]

Identifier: 119896830

GI number: 119896830

Start: 572230

End: 573705

Strand: Direct

Name: nifD [H]

Synonym: azo0539

Alternate gene names: 119896830

Gene position: 572230-573705 (Clockwise)

Preceding gene: 119896829

Following gene: 119896831

Centisome position: 13.08

GC content: 60.5

Gene sequence:

>1476_bases
ATGTCGAACCTGACCCGTGAAGAAACCGACGCCCTCATCCAGGAGGTGCTGGAGGTTTATCCCGAGAAGGCCAAGAAGGA
TCGCGCCAAGCACCTGGCGGTCAACGATCAGAGCATCACCCAGTCGAAGAAGTGCATCACCTCCAACCGCAAGTCCCTGC
CGGGCGTCATGACCATCCGTGGTTGCGCCTACGCCGGTTCGCGCGGCGTGGTGTGGGGTCCGGTCAAGGACATGATCCAC
ATCTCGCACGGCCCGGTTGGCTGTGGCCAATACTCCCGTGGCGGCCGCCGCAACTACTACGTCGGCACCACCGGCGTGAA
CACCTTCGCCGAAATGAACTTCACCTCGGACTTCCAGGAGAAGGACATCGTTTTCGGCGGTGACAAGAAGCTCGCCCAGG
CCATGGCCGAAATCGAGCAGCTGTTCCCCCTGAACAAGGGTATCTCGGTCCAGTCCGAGTGCCCGATCGGCCTGATCGGC
GACGACATCGAAGCCGTCTCCAAGAAGGCTGCCGCGCAGTACAACAAGGTTGTCGTGCCGGTCCGCTGCGAAGGCTTCCG
TGGCGTGTCCCAGTCCCTGGGCCACCACATCGCCAACGACGCGATCCGTGACTACGTCCTGTCGAACCGCGACGGCAAGG
AATTCGAAAGCACCCCGTACGACGTCACCATCATCGGCGACTACAACATCGGCGGCGACGCCTGGGCTTCCCGTATCTTC
CTCGAAGAAATGGGCCTGCGCGTGATCGCCCAGTGGTCCGGTGACGGCACCATCGCCGAAATGGAGAACACCCCGAAGGC
GAAGCTGAACCTCCTCCACTGCTACCGCTCGATGAACTACATCAGCCGTCACATGGAAGAGAAGTACGGGATTCCCTGGA
TGGAGTACAACTTCTTCGGCCCGACCAAGATCGAAGAATCGCTCCGCCGCATCGCCGCCTTCTTCGACGACTCGATCAAG
GCCAAGTGCGAGGAAGTCATCGCCAAGTACAAGCCGATGATGCAAGCCGTCATCGACAAGTACAAGCCGCGCCTCCAGGG
CAAGCGCGTCATGCTCTACATCGGTGGCCTGCGTCCGCGTCACGTCATCGGCGCGTACGAAGACCTCGGCATGGAAGTCG
TGGGCACGGGTTATGAGTTCGCCCACAACGACGACTACGACCGCACCATCCAGGAAATGGGCAATGCGACCCTGCTGTAC
GACGACGTCACCGGCTTCGAGCTGGAAGAGTTCGTCAAGCGCATCAAGCCCGACCTCGTCGGTTCCGGCATCAAGGAAAA
GTACATCTATCAAAAGATGGGCATTCCCTTCCGTCAGATGCACAGCTGGGACTACTCCGGTCCGTATCACGGTTATGACG
GTTTCGCGATCTTCGCGCGCGACATGGACATGACCCTGAACAACCCGTGCTGGGGCAAGCAGACCCCGCCGTGGAAGAAG
ACCGAAGCGACCGAGGACGTGAAGGCTGCCGCCTGA

Upstream 100 bases:

>100_bases
CTCTACCGAGTGCATCCGGCCGCGGTTCGCCGTGGCCGGACTTGAATCCCGTCTTGAGTGTCGTGCACAGATTGGTGCCG
GCATGAAAAGGAGAAGCAAG

Downstream 100 bases:

>100_bases
TCGACCTTCCGGATGGACCTGGTGCGCCGGGGTCGGCGCACCAGGTATCGAATGCACTCAAGCCCGTGTCCACAGATTGC
TGGCCGGGTTAAGGAGCTGA

Product: nitrogenase molybdenum-iron protein alpha chain

Products: NA

Alternate protein names: Dinitrogenase; Nitrogenase component I [H]

Number of amino acids: Translated: 491; Mature: 490

Protein sequence:

>491_residues
MSNLTREETDALIQEVLEVYPEKAKKDRAKHLAVNDQSITQSKKCITSNRKSLPGVMTIRGCAYAGSRGVVWGPVKDMIH
ISHGPVGCGQYSRGGRRNYYVGTTGVNTFAEMNFTSDFQEKDIVFGGDKKLAQAMAEIEQLFPLNKGISVQSECPIGLIG
DDIEAVSKKAAAQYNKVVVPVRCEGFRGVSQSLGHHIANDAIRDYVLSNRDGKEFESTPYDVTIIGDYNIGGDAWASRIF
LEEMGLRVIAQWSGDGTIAEMENTPKAKLNLLHCYRSMNYISRHMEEKYGIPWMEYNFFGPTKIEESLRRIAAFFDDSIK
AKCEEVIAKYKPMMQAVIDKYKPRLQGKRVMLYIGGLRPRHVIGAYEDLGMEVVGTGYEFAHNDDYDRTIQEMGNATLLY
DDVTGFELEEFVKRIKPDLVGSGIKEKYIYQKMGIPFRQMHSWDYSGPYHGYDGFAIFARDMDMTLNNPCWGKQTPPWKK
TEATEDVKAAA

Sequences:

>Translated_491_residues
MSNLTREETDALIQEVLEVYPEKAKKDRAKHLAVNDQSITQSKKCITSNRKSLPGVMTIRGCAYAGSRGVVWGPVKDMIH
ISHGPVGCGQYSRGGRRNYYVGTTGVNTFAEMNFTSDFQEKDIVFGGDKKLAQAMAEIEQLFPLNKGISVQSECPIGLIG
DDIEAVSKKAAAQYNKVVVPVRCEGFRGVSQSLGHHIANDAIRDYVLSNRDGKEFESTPYDVTIIGDYNIGGDAWASRIF
LEEMGLRVIAQWSGDGTIAEMENTPKAKLNLLHCYRSMNYISRHMEEKYGIPWMEYNFFGPTKIEESLRRIAAFFDDSIK
AKCEEVIAKYKPMMQAVIDKYKPRLQGKRVMLYIGGLRPRHVIGAYEDLGMEVVGTGYEFAHNDDYDRTIQEMGNATLLY
DDVTGFELEEFVKRIKPDLVGSGIKEKYIYQKMGIPFRQMHSWDYSGPYHGYDGFAIFARDMDMTLNNPCWGKQTPPWKK
TEATEDVKAAA
>Mature_490_residues
SNLTREETDALIQEVLEVYPEKAKKDRAKHLAVNDQSITQSKKCITSNRKSLPGVMTIRGCAYAGSRGVVWGPVKDMIHI
SHGPVGCGQYSRGGRRNYYVGTTGVNTFAEMNFTSDFQEKDIVFGGDKKLAQAMAEIEQLFPLNKGISVQSECPIGLIGD
DIEAVSKKAAAQYNKVVVPVRCEGFRGVSQSLGHHIANDAIRDYVLSNRDGKEFESTPYDVTIIGDYNIGGDAWASRIFL
EEMGLRVIAQWSGDGTIAEMENTPKAKLNLLHCYRSMNYISRHMEEKYGIPWMEYNFFGPTKIEESLRRIAAFFDDSIKA
KCEEVIAKYKPMMQAVIDKYKPRLQGKRVMLYIGGLRPRHVIGAYEDLGMEVVGTGYEFAHNDDYDRTIQEMGNATLLYD
DVTGFELEEFVKRIKPDLVGSGIKEKYIYQKMGIPFRQMHSWDYSGPYHGYDGFAIFARDMDMTLNNPCWGKQTPPWKKT
EATEDVKAAA

Specific function: This molybdenum-iron protein is part of the nitrogenase complex that catalyzes the key enzymatic reactions in nitrogen fixation [H]

COG id: COG2710

COG function: function code C; Nitrogenase molybdenum-iron protein, alpha and beta chains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifD/nifK/nifE/nifN family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000510
- InterPro:   IPR010143
- InterPro:   IPR000318
- InterPro:   IPR005972 [H]

Pfam domain/function: PF00148 Oxidored_nitro [H]

EC number: =1.18.6.1 [H]

Molecular weight: Translated: 55360; Mature: 55228

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: PS00699 NITROGENASE_1_1 ; PS00090 NITROGENASE_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNLTREETDALIQEVLEVYPEKAKKDRAKHLAVNDQSITQSKKCITSNRKSLPGVMTIR
CCCCCHHHHHHHHHHHHHHCCHHHHHHHHHEEECCCHHHHHHHHHHHCCCCCCCCEEEEE
GCAYAGSRGVVWGPVKDMIHISHGPVGCGQYSRGGRRNYYVGTTGVNTFAEMNFTSDFQE
EEEECCCCCCEECCHHHHEEECCCCCCCCCCCCCCCCEEEEECCCCCHHEECCCCCCCCC
KDIVFGGDKKLAQAMAEIEQLFPLNKGISVQSECPIGLIGDDIEAVSKKAAAQYNKVVVP
CCEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHCCEEEE
VRCEGFRGVSQSLGHHIANDAIRDYVLSNRDGKEFESTPYDVTIIGDYNIGGDAWASRIF
EECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHH
LEEMGLRVIAQWSGDGTIAEMENTPKAKLNLLHCYRSMNYISRHMEEKYGIPWMEYNFFG
HHHCCCEEEEEECCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCC
PTKIEESLRRIAAFFDDSIKAKCEEVIAKYKPMMQAVIDKYKPRLQGKRVMLYIGGLRPR
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCH
HVIGAYEDLGMEVVGTGYEFAHNDDYDRTIQEMGNATLLYDDVTGFELEEFVKRIKPDLV
HHHCCHHHCCCEEEECCEECCCCCCHHHHHHHCCCEEEEEECCCCCCHHHHHHHHCHHHH
GSGIKEKYIYQKMGIPFRQMHSWDYSGPYHGYDGFAIFARDMDMTLNNPCWGKQTPPWKK
CCCHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCC
TEATEDVKAAA
CCCCCHHHCCC
>Mature Secondary Structure 
SNLTREETDALIQEVLEVYPEKAKKDRAKHLAVNDQSITQSKKCITSNRKSLPGVMTIR
CCCCHHHHHHHHHHHHHHCCHHHHHHHHHEEECCCHHHHHHHHHHHCCCCCCCCEEEEE
GCAYAGSRGVVWGPVKDMIHISHGPVGCGQYSRGGRRNYYVGTTGVNTFAEMNFTSDFQE
EEEECCCCCCEECCHHHHEEECCCCCCCCCCCCCCCCEEEEECCCCCHHEECCCCCCCCC
KDIVFGGDKKLAQAMAEIEQLFPLNKGISVQSECPIGLIGDDIEAVSKKAAAQYNKVVVP
CCEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHCCEEEE
VRCEGFRGVSQSLGHHIANDAIRDYVLSNRDGKEFESTPYDVTIIGDYNIGGDAWASRIF
EECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHH
LEEMGLRVIAQWSGDGTIAEMENTPKAKLNLLHCYRSMNYISRHMEEKYGIPWMEYNFFG
HHHCCCEEEEEECCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCC
PTKIEESLRRIAAFFDDSIKAKCEEVIAKYKPMMQAVIDKYKPRLQGKRVMLYIGGLRPR
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCH
HVIGAYEDLGMEVVGTGYEFAHNDDYDRTIQEMGNATLLYDDVTGFELEEFVKRIKPDLV
HHHCCHHHCCCEEEECCEECCCCCCHHHHHHHCCCEEEEEECCCCCCHHHHHHHHCHHHH
GSGIKEKYIYQKMGIPFRQMHSWDYSGPYHGYDGFAIFARDMDMTLNNPCWGKQTPPWKK
CCCHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCC
TEATEDVKAAA
CCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2644218; 3344210; 3863780; 649581; 9163420; 9063865; 11170380; 11951047; 12215645 [H]