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The map label for this gene is rnfC1 [H]

Identifier: 119896806

GI number: 119896806

Start: 549991

End: 551550

Strand: Reverse

Name: rnfC1 [H]

Synonym: azo0515

Alternate gene names: 119896806

Gene position: 551550-549991 (Counterclockwise)

Preceding gene: 119896807

Following gene: 119896805

Centisome position: 12.6

GC content: 71.15

Gene sequence:

>1560_bases
ATGGGCTCGCCCTCCATTCCGCCCCTTGCCCGTCTCGCCCGCCTGCTCAATCCCTGGGGCGTGCACCCCGAAGGCCGCAA
GCAGCTCACCGCCGACACCGAGATCGCGCTGGTGCCGCTGCCGGATCGCCTGGTGCTGCCGCTCAGCCAGCACATCGGCG
CGCCCGCGCGGCCCATCGTCGAGGTCGGCACCCGGGTGCTGCGCAACCAGTTGCTGGCCGAAGCGCAGGGCGCAATCTCG
GCGCCGATCCACGCCCCGACCTCGGGCATCGTGGTCGGCATCGCCGAAGTGCCGGTGCCCCATCCCTCCGGCCTGCCCGG
GCCGGCGATCCTGCTCGAGCCGGATGGCGCCGATGAGGCGCTGCCGGCCGAGCACAACGACCCGTTTGCGCTCAGCGCCG
CCGAGATTTCCCGCCGCGTCGCCGAGGCCGGCATCGTCGGCATGGGCGGCGCCACCTTCCCGGCGGCGGTCAAGCTGTCG
CTCGGCCAGAAGACCGCGATCCCGACGCTGATCCTCAACGGCGGCGAATGCGAACCCTACCTGACCTGTGACGACCGCCT
GATGCGCGACCGCGCCGATCAGGTGATCGACGGCGCGCGCATCATCCTGCACGCGATGGGCGGCCGCGAGGTGCTGATCG
GTGTCGAGAACAACAAGCCGGTGGCGATCGCGGCGCTGGAAGCCGCGGCCGGCGCCTTCCCCGAGGTGAAGGTGGTGGCG
GTGCCCAGCCGCTACCCCATGGGCTCGGAGAAGCAGCTGATCGAATGGCTTACCGGGCGCGAGATTCCCGCCGGCGGGCG
CCCCGCCGACATCGGCGTGATGGTGCAGAACGTCGGCACCGCGGCGGCCATCCACCGCGCGATCCGCTTCGGCGAGCCGC
TGACGCGCCGCATCGTCACCGTGTCCGGTGGCGGGGTGCGCACCCCGCGCAACGTCGAAGTCCGCATCGGCACGCCCTTG
TCGGCGCTGCTGGCCTTCTGCGGCGGCCTCAAGGCCGAGGCGGTGCGCTATGTGATGGGCGGCCCGATGATGGGCCTCGC
GGTCCAGTCGCTGGAGATTCCGGTCATCAAGGGCAGCGGCGGCCTGCTCGCGCTGGTCGCGGGCGAAGTCGGCAGCTACG
GCACCGAGGACACCAGCGCCGGCCCCTGCATCCGCTGCGCCTCCTGCGTCGGCGCCTGCCCGATCGGCCTGATGCCGCTC
GACATGGCCGCGCTGATCAAGCACGGCGACCTCGCCGCCTCGGTCGATATCGGCCTCAAGGACTGCATCGGCTGCGGCAC
CTGCTCCTACGTCTGCCCGAGCAAGATTCCGCTGGTGCATTACTTCAACCACGCCAAGGGCGAACTCGCCGCGCAGGATC
GCAACAAGATGAAGCAGGACGCGATCAAGCAGCTGGCCGATGCACGCACCGCGCGCATGGAACAGGAAGCGCGCGAGAAG
GCCGAGGCCGCCGCGCGCCGCAAGGCCGAACGTGAAAAGGCCAAGGCCGAAGCCGCCGCCAAGGCCGCTGCCAAGAAGGC
CGCCGCACCCGCACCCGCCAGCGAAGAGAGCCCAGCATGA

Upstream 100 bases:

>100_bases
GAGGACGTCTGCCCCACCGGCGCGATCACGCTGTCGCCCATCCCGGTCACCCAGCAGAGCTGGGTGTGGCCCAAGCCTGT
CGCCGCCTGAGGATACGAAC

Downstream 100 bases:

>100_bases
ACGCGATTTCCTCCCCCCACGCCCACGGCGCGCGCAAGGTCGGCCGGGTCATGACGCTGGTGATGCTGGCGCTGGTGCCC
GCCACGCTGGCCGGCTTCTG

Product: electron transport complex protein RnfC

Products: NA

Alternate protein names: Nitrogen fixation protein rnfC [H]

Number of amino acids: Translated: 519; Mature: 518

Protein sequence:

>519_residues
MGSPSIPPLARLARLLNPWGVHPEGRKQLTADTEIALVPLPDRLVLPLSQHIGAPARPIVEVGTRVLRNQLLAEAQGAIS
APIHAPTSGIVVGIAEVPVPHPSGLPGPAILLEPDGADEALPAEHNDPFALSAAEISRRVAEAGIVGMGGATFPAAVKLS
LGQKTAIPTLILNGGECEPYLTCDDRLMRDRADQVIDGARIILHAMGGREVLIGVENNKPVAIAALEAAAGAFPEVKVVA
VPSRYPMGSEKQLIEWLTGREIPAGGRPADIGVMVQNVGTAAAIHRAIRFGEPLTRRIVTVSGGGVRTPRNVEVRIGTPL
SALLAFCGGLKAEAVRYVMGGPMMGLAVQSLEIPVIKGSGGLLALVAGEVGSYGTEDTSAGPCIRCASCVGACPIGLMPL
DMAALIKHGDLAASVDIGLKDCIGCGTCSYVCPSKIPLVHYFNHAKGELAAQDRNKMKQDAIKQLADARTARMEQEAREK
AEAAARRKAEREKAKAEAAAKAAAKKAAAPAPASEESPA

Sequences:

>Translated_519_residues
MGSPSIPPLARLARLLNPWGVHPEGRKQLTADTEIALVPLPDRLVLPLSQHIGAPARPIVEVGTRVLRNQLLAEAQGAIS
APIHAPTSGIVVGIAEVPVPHPSGLPGPAILLEPDGADEALPAEHNDPFALSAAEISRRVAEAGIVGMGGATFPAAVKLS
LGQKTAIPTLILNGGECEPYLTCDDRLMRDRADQVIDGARIILHAMGGREVLIGVENNKPVAIAALEAAAGAFPEVKVVA
VPSRYPMGSEKQLIEWLTGREIPAGGRPADIGVMVQNVGTAAAIHRAIRFGEPLTRRIVTVSGGGVRTPRNVEVRIGTPL
SALLAFCGGLKAEAVRYVMGGPMMGLAVQSLEIPVIKGSGGLLALVAGEVGSYGTEDTSAGPCIRCASCVGACPIGLMPL
DMAALIKHGDLAASVDIGLKDCIGCGTCSYVCPSKIPLVHYFNHAKGELAAQDRNKMKQDAIKQLADARTARMEQEAREK
AEAAARRKAEREKAKAEAAAKAAAKKAAAPAPASEESPA
>Mature_518_residues
GSPSIPPLARLARLLNPWGVHPEGRKQLTADTEIALVPLPDRLVLPLSQHIGAPARPIVEVGTRVLRNQLLAEAQGAISA
PIHAPTSGIVVGIAEVPVPHPSGLPGPAILLEPDGADEALPAEHNDPFALSAAEISRRVAEAGIVGMGGATFPAAVKLSL
GQKTAIPTLILNGGECEPYLTCDDRLMRDRADQVIDGARIILHAMGGREVLIGVENNKPVAIAALEAAAGAFPEVKVVAV
PSRYPMGSEKQLIEWLTGREIPAGGRPADIGVMVQNVGTAAAIHRAIRFGEPLTRRIVTVSGGGVRTPRNVEVRIGTPLS
ALLAFCGGLKAEAVRYVMGGPMMGLAVQSLEIPVIKGSGGLLALVAGEVGSYGTEDTSAGPCIRCASCVGACPIGLMPLD
MAALIKHGDLAASVDIGLKDCIGCGTCSYVCPSKIPLVHYFNHAKGELAAQDRNKMKQDAIKQLADARTARMEQEAREKA
EAAARRKAEREKAKAEAAAKAAAKKAAAPAPASEESPA

Specific function: Required for nitrogen fixation. May be part of a membrane complex functioning as an intermediate in the electron transport to nitrogenase. Stabilizes rnfB [H]

COG id: COG4656

COG function: function code C; Predicted NADH:ubiquinone oxidoreductase, subunit RnfC

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Probable) [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787916, Length=471, Percent_Identity=42.6751592356688, Blast_Score=367, Evalue=1e-103,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR010208
- InterPro:   IPR012285
- InterPro:   IPR009051
- InterPro:   IPR011538
- InterPro:   IPR019554 [H]

Pfam domain/function: PF01512 Complex1_51K; PF10531 SLBB [H]

EC number: NA

Molecular weight: Translated: 53808; Mature: 53677

Theoretical pI: Translated: 7.69; Mature: 7.69

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSPSIPPLARLARLLNPWGVHPEGRKQLTADTEIALVPLPDRLVLPLSQHIGAPARPIV
CCCCCCCHHHHHHHHHCCCCCCCCCCCEEECCCEEEEEECCCHHCCCHHHHCCCCCCHHH
EVGTRVLRNQLLAEAQGAISAPIHAPTSGIVVGIAEVPVPHPSGLPGPAILLEPDGADEA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCEEEECCCCCCCC
LPAEHNDPFALSAAEISRRVAEAGIVGMGGATFPAAVKLSLGQKTAIPTLILNGGECEPY
CCCCCCCCCEEEHHHHHHHHHHCCEEECCCCCCCCEEEEECCCCCCCCEEEEECCCCCCE
LTCDDRLMRDRADQVIDGARIILHAMGGREVLIGVENNKPVAIAALEAAAGAFPEVKVVA
ECCHHHHHHHHHHHHHCCCEEEEEECCCCEEEEEECCCCCEEEEEEHHHCCCCCCEEEEE
VPSRYPMGSEKQLIEWLTGREIPAGGRPADIGVMVQNVGTAAAIHRAIRFGEPLTRRIVT
ECCCCCCCCHHHHHHHHCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHCCCCHHEEEEE
VSGGGVRTPRNVEVRIGTPLSALLAFCGGLKAEAVRYVMGGPMMGLAVQSLEIPVIKGSG
ECCCCCCCCCCEEEEECCCHHHHHHHHCCCHHHHHHHHHCCCHHHHEEHEEEEEEEECCC
GLLALVAGEVGSYGTEDTSAGPCIRCASCVGACPIGLMPLDMAALIKHGDLAASVDIGLK
CEEEEEECCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHCCCCEEEECCCHH
DCIGCGTCSYVCPSKIPLVHYFNHAKGELAAQDRNKMKQDAIKQLADARTARMEQEAREK
HHHCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AEAAARRKAEREKAKAEAAAKAAAKKAAAPAPASEESPA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
GSPSIPPLARLARLLNPWGVHPEGRKQLTADTEIALVPLPDRLVLPLSQHIGAPARPIV
CCCCCCHHHHHHHHHCCCCCCCCCCCEEECCCEEEEEECCCHHCCCHHHHCCCCCCHHH
EVGTRVLRNQLLAEAQGAISAPIHAPTSGIVVGIAEVPVPHPSGLPGPAILLEPDGADEA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCEEEECCCCCCCC
LPAEHNDPFALSAAEISRRVAEAGIVGMGGATFPAAVKLSLGQKTAIPTLILNGGECEPY
CCCCCCCCCEEEHHHHHHHHHHCCEEECCCCCCCCEEEEECCCCCCCCEEEEECCCCCCE
LTCDDRLMRDRADQVIDGARIILHAMGGREVLIGVENNKPVAIAALEAAAGAFPEVKVVA
ECCHHHHHHHHHHHHHCCCEEEEEECCCCEEEEEECCCCCEEEEEEHHHCCCCCCEEEEE
VPSRYPMGSEKQLIEWLTGREIPAGGRPADIGVMVQNVGTAAAIHRAIRFGEPLTRRIVT
ECCCCCCCCHHHHHHHHCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHCCCCHHEEEEE
VSGGGVRTPRNVEVRIGTPLSALLAFCGGLKAEAVRYVMGGPMMGLAVQSLEIPVIKGSG
ECCCCCCCCCCEEEEECCCHHHHHHHHCCCHHHHHHHHHCCCHHHHEEHEEEEEEEECCC
GLLALVAGEVGSYGTEDTSAGPCIRCASCVGACPIGLMPLDMAALIKHGDLAASVDIGLK
CEEEEEECCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHCCCCEEEECCCHH
DCIGCGTCSYVCPSKIPLVHYFNHAKGELAAQDRNKMKQDAIKQLADARTARMEQEAREK
HHHCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AEAAARRKAEREKAKAEAAAKAAAKKAAAPAPASEESPA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: 4Fe-4S clusters [C]

Metal ions: Iron-Sulfur [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8264535; 9492268; 9154934 [H]