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The map label for this gene is ttcA

Identifier: 119896776

GI number: 119896776

Start: 523396

End: 524316

Strand: Reverse

Name: ttcA

Synonym: azo0485

Alternate gene names: 119896776

Gene position: 524316-523396 (Counterclockwise)

Preceding gene: 119896777

Following gene: 119896775

Centisome position: 11.98

GC content: 65.36

Gene sequence:

>921_bases
TTGCCTGCCGTCGCGCCCAGCGCCGCCGAATCCCGCCATTCCAATACCTTTCTGCGCCTGAAGAAGAAGCTGGAGCGCGG
CGTCGGCGAGGCCATCGCCGACTACAACATGATCGGCGACGGCGACACCGTGATGGTGTGCGTGTCGGGCGGCAAGGACT
CCTACACGCTGCTGTCCTGCCTGCTGGCGCTGCGCGAACGCGCCCCGGTGGATTTCCGCATCGTCGCGATGAACCTGGAC
CAGAAGCAGCCCGGCTTCCCGGACGACGTGCTGCCCGCCTACTTCGAATCGATCGGCGTCGAGTACCGCATCGTCACCGA
AGACACGTATTCCATCGTCAAGGACAAGATTCCGGAAGGAAAGACCACCTGCTCGCTGTGCTCGCGCCTGCGGCGCGGCA
TCATCTACCGCACGGCAAAGGAAATCGGCGCCACCCGGATCGCGCTCGGTCACCACCGCGACGACATGCTGGAAACGCTC
TTCCTCAACATGTTCTTCGGCGGCAAGATCAAGGCCATGCCGCCCAAACTGGTCAGTGACGACGGCCAGCACGTCGTGAT
CCGCCCGCTCGCCTACTGCACCGAAAACGACATCGAGCGCTTCGCGCGCGGCATGGACTTCCCGATCATCCCGTGCAACC
TGTGCGGCTCGCAGGAAAACGCGCAGCGCAAGCAGATCAAGACGATGCTGCAAGGCTGGGCCAGGGACTACCCCGGGCGC
ATCGAATCGCTCGCAACCTCGCTGCGCAATGTGGTGCCGTCGCACCTGTCGGACAGCGCGCTGTTCGACTTCGTCGGCCT
CACCCGTGACACCCGGGTGGGTGAAGGCGATACCGTGTTCGACCCGCCGGAACTGCCCGCGGCAGCGGCGCCGATCACCC
TGCGGCGCGCCGACGACGACGGGGACCGCAGCGCGACATAG

Upstream 100 bases:

>100_bases
GGACGGCGGTCGCAGTGCCCATCTTTGAGGCAGGCGGGCGGGGGCGCGGGCGTATAATCCCGCCATCCCATTCCGAGCAG
TTCCAGTGAACACCGCCGCA

Downstream 100 bases:

>100_bases
ACCGTTGCCATTTCGGCTTGCGTGCCCGTCATCATTTGATCATCATGAGCCGCAGCGCCGACACCGGCCTACCCGCTCCA
CAACAATAACGCCACCATGT

Product: C32 tRNA thiolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 306; Mature: 305

Protein sequence:

>306_residues
MPAVAPSAAESRHSNTFLRLKKKLERGVGEAIADYNMIGDGDTVMVCVSGGKDSYTLLSCLLALRERAPVDFRIVAMNLD
QKQPGFPDDVLPAYFESIGVEYRIVTEDTYSIVKDKIPEGKTTCSLCSRLRRGIIYRTAKEIGATRIALGHHRDDMLETL
FLNMFFGGKIKAMPPKLVSDDGQHVVIRPLAYCTENDIERFARGMDFPIIPCNLCGSQENAQRKQIKTMLQGWARDYPGR
IESLATSLRNVVPSHLSDSALFDFVGLTRDTRVGEGDTVFDPPELPAAAAPITLRRADDDGDRSAT

Sequences:

>Translated_306_residues
MPAVAPSAAESRHSNTFLRLKKKLERGVGEAIADYNMIGDGDTVMVCVSGGKDSYTLLSCLLALRERAPVDFRIVAMNLD
QKQPGFPDDVLPAYFESIGVEYRIVTEDTYSIVKDKIPEGKTTCSLCSRLRRGIIYRTAKEIGATRIALGHHRDDMLETL
FLNMFFGGKIKAMPPKLVSDDGQHVVIRPLAYCTENDIERFARGMDFPIIPCNLCGSQENAQRKQIKTMLQGWARDYPGR
IESLATSLRNVVPSHLSDSALFDFVGLTRDTRVGEGDTVFDPPELPAAAAPITLRRADDDGDRSAT
>Mature_305_residues
PAVAPSAAESRHSNTFLRLKKKLERGVGEAIADYNMIGDGDTVMVCVSGGKDSYTLLSCLLALRERAPVDFRIVAMNLDQ
KQPGFPDDVLPAYFESIGVEYRIVTEDTYSIVKDKIPEGKTTCSLCSRLRRGIIYRTAKEIGATRIALGHHRDDMLETLF
LNMFFGGKIKAMPPKLVSDDGQHVVIRPLAYCTENDIERFARGMDFPIIPCNLCGSQENAQRKQIKTMLQGWARDYPGRI
ESLATSLRNVVPSHLSDSALFDFVGLTRDTRVGEGDTVFDPPELPAAAAPITLRRADDDGDRSAT

Specific function: Required for the thiolation of cytidine in position 32 of tRNA, to form 2-thiocytidine (s(2)C32)

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TtcA family

Homologues:

Organism=Escherichia coli, GI1787606, Length=273, Percent_Identity=66.3003663003663, Blast_Score=396, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI193211090, Length=244, Percent_Identity=29.9180327868852, Blast_Score=113, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17540090, Length=235, Percent_Identity=29.7872340425532, Blast_Score=88, Evalue=5e-18,
Organism=Drosophila melanogaster, GI24651972, Length=234, Percent_Identity=26.9230769230769, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TTCA_AZOSB (A1K2P7)

Other databases:

- EMBL:   AM406670
- RefSeq:   YP_931989.1
- ProteinModelPortal:   A1K2P7
- STRING:   A1K2P7
- GeneID:   4606423
- GenomeReviews:   AM406670_GR
- KEGG:   azo:azo0485
- eggNOG:   COG0037
- HOGENOM:   HBG347038
- OMA:   FPHAREE
- PhylomeDB:   A1K2P7
- ProtClustDB:   PRK10696
- BioCyc:   ASP62928:AZO0485-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01850
- InterPro:   IPR012089
- InterPro:   IPR014729
- InterPro:   IPR011063
- Gene3D:   G3DSA:3.40.50.620
- PIRSF:   PIRSF004976

Pfam domain/function: PF01171 ATP_bind_3

EC number: NA

Molecular weight: Translated: 33816; Mature: 33685

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAVAPSAAESRHSNTFLRLKKKLERGVGEAIADYNMIGDGDTVMVCVSGGKDSYTLLSC
CCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHCCEECCCCEEEEEEECCCCHHHHHHH
LLALRERAPVDFRIVAMNLDQKQPGFPDDVLPAYFESIGVEYRIVTEDTYSIVKDKIPEG
HHHHHHCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHCCEEEEEECHHHHHHHHCCCCC
KTTCSLCSRLRRGIIYRTAKEIGATRIALGHHRDDMLETLFLNMFFGGKIKAMPPKLVSD
CHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHCCCEEECCCCHHHCC
DGQHVVIRPLAYCTENDIERFARGMDFPIIPCNLCGSQENAQRKQIKTMLQGWARDYPGR
CCCEEEEEHHHHHCHHHHHHHHCCCCCCEEEHHCCCCCCHHHHHHHHHHHHHHHHCCCHH
IESLATSLRNVVPSHLSDSALFDFVGLTRDTRVGEGDTVFDPPELPAAAAPITLRRADDD
HHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCC
GDRSAT
CCCCCC
>Mature Secondary Structure 
PAVAPSAAESRHSNTFLRLKKKLERGVGEAIADYNMIGDGDTVMVCVSGGKDSYTLLSC
CCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHCCEECCCCEEEEEEECCCCHHHHHHH
LLALRERAPVDFRIVAMNLDQKQPGFPDDVLPAYFESIGVEYRIVTEDTYSIVKDKIPEG
HHHHHHCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHCCEEEEEECHHHHHHHHCCCCC
KTTCSLCSRLRRGIIYRTAKEIGATRIALGHHRDDMLETLFLNMFFGGKIKAMPPKLVSD
CHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHCCCEEECCCCHHHCC
DGQHVVIRPLAYCTENDIERFARGMDFPIIPCNLCGSQENAQRKQIKTMLQGWARDYPGR
CCCEEEEEHHHHHCHHHHHHHHCCCCCCEEEHHCCCCCCHHHHHHHHHHHHHHHHCCCHH
IESLATSLRNVVPSHLSDSALFDFVGLTRDTRVGEGDTVFDPPELPAAAAPITLRRADDD
HHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCC
GDRSAT
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA