| Definition | Azoarcus sp. BH72 chromosome, complete genome. |
|---|---|
| Accession | NC_008702 |
| Length | 4,376,040 |
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The map label for this gene is gra-orf6 [H]
Identifier: 119896777
GI number: 119896777
Start: 524389
End: 525135
Strand: Reverse
Name: gra-orf6 [H]
Synonym: azo0486
Alternate gene names: 119896777
Gene position: 525135-524389 (Counterclockwise)
Preceding gene: 119896779
Following gene: 119896776
Centisome position: 12.0
GC content: 71.49
Gene sequence:
>747_bases ATGGACACCGCAGAAGCCCCCGTCATCCTCGTCACCGGCGCCGCACGCCGCGTGGGCGCCGAGATCGCCCGCCTCGTGCA TGCGAGCGGCGCGCGCGTCATCGTCCACCACCGCCGCTCTGCAGCCGAGGCGGAGACGCTGGCTGCAGGGCTGAACGCCC TTCGCCCCGGCTCCGCCGCGGTGGTGGCCGGCGACCTCGCTGCGGATGGCGAGCCGGCGCGCGTCGCAGCCGCTGCGCTC GACTGCTTCGGTCGCATCGACGGCCTGGTGAACAACGCGTCCAGCTTCTTCCCGACCGCGCTCGGCACGATCGACGAAAC GGCCTGGAGCGATCTCGTCGGCTCCAACCTCAAGGGTCCGCTCTTCCTCAGCCAGGCGCTCGCGGCAGAACTCACGCGCC GCGCCGGCAGCATCGTGAACATCGTGGACATTCACGCCGAGCGGCCGCTGAAGGGCTATCTGCTGTATTGCACCGCGAAG GCCGGGCTGGTGGGCCTGACGCGCGCGCTGGCGGTTGAACTGGCGCCGGCGGTACGGGTGAACGGCGTCGCGCCGGGGCC GATCGTATGGCCGGAGGACACACAGTTCGACGCCGAGGCGCGCGCGCAGATCGTCCGCCACACGCTGTTGCAGCGCGAAG GCGCGCCGGCCGACATCGCCCGCGCAGTCCGCTTCCTGCTGGTCGATGCGCCCTACGTCACCGGCCAGATCCTGGCGGTG GACGGCGGTCGCAGTGCCCATCTTTGA
Upstream 100 bases:
>100_bases GATTTCGAAGAAGGCGGCATTGTATCGCCCGCGCCCGCGCCGCCGCCGATTCGGCAACGGGGTTTGCAGCGACGCCGCGA GGCCCGACAATGGCGGACCC
Downstream 100 bases:
>100_bases GGCAGGCGGGCGGGGGCGCGGGCGTATAATCCCGCCATCCCATTCCGAGCAGTTCCAGTGAACACCGCCGCATTGCCTGC CGTCGCGCCCAGCGCCGCCG
Product: pteridine reductase
Products: (3R)-hydroxyacyl-[acyl-carrier-protein]; NADP; NADPH; Proton; beta-ketoacyl-ACP [C]
Alternate protein names: ORF6 [H]
Number of amino acids: Translated: 248; Mature: 248
Protein sequence:
>248_residues MDTAEAPVILVTGAARRVGAEIARLVHASGARVIVHHRRSAAEAETLAAGLNALRPGSAAVVAGDLAADGEPARVAAAAL DCFGRIDGLVNNASSFFPTALGTIDETAWSDLVGSNLKGPLFLSQALAAELTRRAGSIVNIVDIHAERPLKGYLLYCTAK AGLVGLTRALAVELAPAVRVNGVAPGPIVWPEDTQFDAEARAQIVRHTLLQREGAPADIARAVRFLLVDAPYVTGQILAV DGGRSAHL
Sequences:
>Translated_248_residues MDTAEAPVILVTGAARRVGAEIARLVHASGARVIVHHRRSAAEAETLAAGLNALRPGSAAVVAGDLAADGEPARVAAAAL DCFGRIDGLVNNASSFFPTALGTIDETAWSDLVGSNLKGPLFLSQALAAELTRRAGSIVNIVDIHAERPLKGYLLYCTAK AGLVGLTRALAVELAPAVRVNGVAPGPIVWPEDTQFDAEARAQIVRHTLLQREGAPADIARAVRFLLVDAPYVTGQILAV DGGRSAHL >Mature_248_residues MDTAEAPVILVTGAARRVGAEIARLVHASGARVIVHHRRSAAEAETLAAGLNALRPGSAAVVAGDLAADGEPARVAAAAL DCFGRIDGLVNNASSFFPTALGTIDETAWSDLVGSNLKGPLFLSQALAAELTRRAGSIVNIVDIHAERPLKGYLLYCTAK AGLVGLTRALAVELAPAVRVNGVAPGPIVWPEDTQFDAEARAQIVRHTLLQREGAPADIARAVRFLLVDAPYVTGQILAV DGGRSAHL
Specific function: Fatty acid biosynthesis pathway; first reduction step. [C]
COG id: COG1028
COG function: function code IQR; Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases)
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]
Homologues:
Organism=Homo sapiens, GI32483357, Length=251, Percent_Identity=33.0677290836653, Blast_Score=103, Evalue=1e-22, Organism=Homo sapiens, GI19923817, Length=255, Percent_Identity=29.8039215686275, Blast_Score=95, Evalue=5e-20, Organism=Homo sapiens, GI5031737, Length=244, Percent_Identity=32.7868852459016, Blast_Score=91, Evalue=8e-19, Organism=Homo sapiens, GI126723750, Length=259, Percent_Identity=29.3436293436293, Blast_Score=86, Evalue=4e-17, Organism=Homo sapiens, GI40254992, Length=243, Percent_Identity=31.6872427983539, Blast_Score=81, Evalue=7e-16, Organism=Homo sapiens, GI7705925, Length=241, Percent_Identity=29.4605809128631, Blast_Score=77, Evalue=1e-14, Organism=Homo sapiens, GI10190704, Length=255, Percent_Identity=32.5490196078431, Blast_Score=76, Evalue=3e-14, Organism=Homo sapiens, GI15277342, Length=244, Percent_Identity=30.7377049180328, Blast_Score=74, Evalue=1e-13, Organism=Homo sapiens, GI33667109, Length=211, Percent_Identity=31.2796208530806, Blast_Score=74, Evalue=1e-13, Organism=Homo sapiens, GI59889578, Length=225, Percent_Identity=28.8888888888889, Blast_Score=69, Evalue=3e-12, Organism=Homo sapiens, GI126723191, Length=187, Percent_Identity=32.0855614973262, Blast_Score=69, Evalue=3e-12, Organism=Homo sapiens, GI31542939, Length=187, Percent_Identity=29.4117647058824, Blast_Score=64, Evalue=9e-11, Organism=Escherichia coli, GI1787335, Length=250, Percent_Identity=33.6, Blast_Score=97, Evalue=8e-22, Organism=Escherichia coli, GI1788459, Length=244, Percent_Identity=31.1475409836066, Blast_Score=87, Evalue=8e-19, Organism=Escherichia coli, GI1787891, Length=239, Percent_Identity=27.1966527196653, Blast_Score=79, Evalue=2e-16, Organism=Escherichia coli, GI1789208, Length=181, Percent_Identity=33.7016574585635, Blast_Score=77, Evalue=7e-16, Organism=Escherichia coli, GI1787905, Length=241, Percent_Identity=31.1203319502075, Blast_Score=75, Evalue=4e-15, Organism=Escherichia coli, GI1790717, Length=245, Percent_Identity=30.2040816326531, Blast_Score=75, Evalue=4e-15, Organism=Escherichia coli, GI87082160, Length=185, Percent_Identity=30.2702702702703, Blast_Score=72, Evalue=3e-14, Organism=Escherichia coli, GI87082100, Length=254, Percent_Identity=31.8897637795276, Blast_Score=68, Evalue=4e-13, Organism=Caenorhabditis elegans, GI115534694, Length=245, Percent_Identity=34.2857142857143, Blast_Score=100, Evalue=7e-22, Organism=Caenorhabditis elegans, GI71994600, Length=249, Percent_Identity=28.9156626506024, Blast_Score=98, Evalue=5e-21, Organism=Caenorhabditis elegans, GI17562910, Length=256, Percent_Identity=32.03125, Blast_Score=92, Evalue=2e-19, Organism=Caenorhabditis elegans, GI17560332, Length=264, Percent_Identity=32.5757575757576, Blast_Score=91, Evalue=5e-19, Organism=Caenorhabditis elegans, GI71994604, Length=251, Percent_Identity=29.0836653386454, Blast_Score=91, Evalue=6e-19, Organism=Caenorhabditis elegans, GI17561402, Length=257, Percent_Identity=31.1284046692607, Blast_Score=89, Evalue=3e-18, Organism=Caenorhabditis elegans, GI17562908, Length=261, Percent_Identity=29.8850574712644, Blast_Score=85, Evalue=3e-17, Organism=Caenorhabditis elegans, GI17562906, Length=267, Percent_Identity=28.4644194756554, Blast_Score=83, Evalue=1e-16, Organism=Caenorhabditis elegans, GI17560676, Length=248, Percent_Identity=29.0322580645161, Blast_Score=81, Evalue=4e-16, Organism=Caenorhabditis elegans, GI17555706, Length=254, Percent_Identity=32.6771653543307, Blast_Score=80, Evalue=9e-16, Organism=Caenorhabditis elegans, GI17531453, Length=262, Percent_Identity=29.0076335877863, Blast_Score=80, Evalue=1e-15, Organism=Caenorhabditis elegans, GI17562904, Length=263, Percent_Identity=30.0380228136882, Blast_Score=80, Evalue=1e-15, Organism=Caenorhabditis elegans, GI193204405, Length=259, Percent_Identity=29.7297297297297, Blast_Score=80, Evalue=1e-15, Organism=Caenorhabditis elegans, GI25147288, Length=243, Percent_Identity=30.0411522633745, Blast_Score=75, Evalue=2e-14, Organism=Caenorhabditis elegans, GI72000259, Length=262, Percent_Identity=27.8625954198473, Blast_Score=75, Evalue=4e-14, Organism=Caenorhabditis elegans, GI17559104, Length=262, Percent_Identity=29.0076335877863, Blast_Score=75, Evalue=4e-14, Organism=Caenorhabditis elegans, GI17538486, Length=267, Percent_Identity=29.9625468164794, Blast_Score=73, Evalue=1e-13, Organism=Caenorhabditis elegans, GI17551412, Length=263, Percent_Identity=29.277566539924, Blast_Score=73, Evalue=1e-13, Organism=Caenorhabditis elegans, GI17560150, Length=260, Percent_Identity=29.2307692307692, Blast_Score=70, Evalue=1e-12, Organism=Caenorhabditis elegans, GI17562990, Length=263, Percent_Identity=29.6577946768061, Blast_Score=70, Evalue=1e-12, Organism=Caenorhabditis elegans, GI17560220, Length=260, Percent_Identity=28.0769230769231, Blast_Score=69, Evalue=3e-12, Organism=Caenorhabditis elegans, GI17563726, Length=255, Percent_Identity=28.2352941176471, Blast_Score=68, Evalue=4e-12, Organism=Saccharomyces cerevisiae, GI6324126, Length=258, Percent_Identity=30.2325581395349, Blast_Score=70, Evalue=2e-13, Organism=Drosophila melanogaster, GI24644339, Length=247, Percent_Identity=31.9838056680162, Blast_Score=104, Evalue=5e-23, Organism=Drosophila melanogaster, GI23397609, Length=246, Percent_Identity=32.1138211382114, Blast_Score=103, Evalue=1e-22, Organism=Drosophila melanogaster, GI21357041, Length=262, Percent_Identity=33.206106870229, Blast_Score=100, Evalue=7e-22, Organism=Drosophila melanogaster, GI28571526, Length=254, Percent_Identity=31.8897637795276, Blast_Score=95, Evalue=4e-20, Organism=Drosophila melanogaster, GI24639444, Length=242, Percent_Identity=29.7520661157025, Blast_Score=78, Evalue=5e-15, Organism=Drosophila melanogaster, GI24644337, Length=196, Percent_Identity=32.6530612244898, Blast_Score=73, Evalue=2e-13, Organism=Drosophila melanogaster, GI24643142, Length=242, Percent_Identity=27.6859504132231, Blast_Score=70, Evalue=1e-12, Organism=Drosophila melanogaster, GI21355319, Length=266, Percent_Identity=28.5714285714286, Blast_Score=68, Evalue=5e-12,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR002198 - InterPro: IPR002347 - InterPro: IPR016040 [H]
Pfam domain/function: PF00106 adh_short [H]
EC number: 1.1.1.100 [C]
Molecular weight: Translated: 25696; Mature: 25696
Theoretical pI: Translated: 6.80; Mature: 6.80
Prosite motif: PS00061 ADH_SHORT
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.8 %Cys (Translated Protein) 0.4 %Met (Translated Protein) 1.2 %Cys+Met (Translated Protein) 0.8 %Cys (Mature Protein) 0.4 %Met (Mature Protein) 1.2 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MDTAEAPVILVTGAARRVGAEIARLVHASGARVIVHHRRSAAEAETLAAGLNALRPGSAA CCCCCCCEEEEECCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCE VVAGDLAADGEPARVAAAALDCFGRIDGLVNNASSFFPTALGTIDETAWSDLVGSNLKGP EEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCH LFLSQALAAELTRRAGSIVNIVDIHAERPLKGYLLYCTAKAGLVGLTRALAVELAPAVRV HHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCEEE NGVAPGPIVWPEDTQFDAEARAQIVRHTLLQREGAPADIARAVRFLLVDAPYVTGQILAV CCCCCCCEECCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCEEEEE DGGRSAHL ECCCCCCC >Mature Secondary Structure MDTAEAPVILVTGAARRVGAEIARLVHASGARVIVHHRRSAAEAETLAAGLNALRPGSAA CCCCCCCEEEEECCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCE VVAGDLAADGEPARVAAAALDCFGRIDGLVNNASSFFPTALGTIDETAWSDLVGSNLKGP EEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCH LFLSQALAAELTRRAGSIVNIVDIHAERPLKGYLLYCTAKAGLVGLTRALAVELAPAVRV HHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCEEE NGVAPGPIVWPEDTQFDAEARAQIVRHTLLQREGAPADIARAVRFLLVDAPYVTGQILAV CCCCCCCEECCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCEEEEE DGGRSAHL ECCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NADPH [C]
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: Oxoacyl-[acyl-carrier-protein]; NADPH; D-3-hydroxy-acyl-ACP; NADP [C]
Specific reaction: Oxoacyl-[acyl-carrier-protein] + NADPH = (3R)-hydroxyacyl-[acyl-carrier-protein] + NADP+ D-3-hydroxy-acyl-ACP + NADP = NADPH + Proton + beta-ketoacyl-ACP [C]
General reaction: Redox reaction [C]
Inhibitor: NA
Structure determination priority: 10.0
TargetDB status: NA
Availability: NA
References: 2583128; 9831526 [H]