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The map label for this gene is recG [H]

Identifier: 119896768

GI number: 119896768

Start: 513369

End: 515408

Strand: Direct

Name: recG [H]

Synonym: azo0477

Alternate gene names: 119896768

Gene position: 513369-515408 (Clockwise)

Preceding gene: 119896767

Following gene: 119896769

Centisome position: 11.73

GC content: 71.72

Gene sequence:

>2040_bases
GTGCAGTCGGGCTGGGCGGGACTGGGCGAGCAGCTCGCCTCGCGCCTGCGCAAGCTGGACCTGCACCGGCAGGAGGACCT
CGTCGTCCACCTGCCGCTGCGCTATGAGGACGAAACCCGGCTGACGCCGATCGGCGTCGCCCGCGCCGGCTTTCCGGCGC
AGATCGAAGGCGAGGTCACCTCCTGCGAAGTCGTGCTGCGCCCCCGCCGCCAGTTGGTGGCGCGGGTGCGCGACGATTCG
GGCACGCTGGTCGCGCGCTGGATCAATTTCTACCCTTCGCAGCAGAAGCAGCTTGCGGTCGGGCGCCGTGTGCGGCTGTT
CGGCGAAGTGCGGGGCGGCTTCTTCGGCGACGAGATGGTGCATCCGCGGGTGCGCAACATCGACCCCGGTGAAGGCTTGC
CGGAGGCGCTGACGCCGGTGTATCCGACCACCGCCGGGCTGGGCCAGACCACGCTGAGGAAGCTGATCGACCGCGCGCTG
AAGACGGTGCCGCTGCAGGATCTGCTGCCGGAAGACGCGCGCCGCGCGCTCGACCTGCCCGGCTTCGCCGAGGCGCTGAC
CGCGCTGCACCACCCGGCGCCGGATGCCGATCCGATCGCGCTGGAGAACCGCGAGCATCCGGCCTGGCGGCGCATCAAGT
TCGAGGAACTGCTGGTGCAGCAGCTCTCGCTGCGGCGCGCCTACAACGCCCGCCGGGCGCGCGAGGCGGTGGTGCTGCCG
GCGCGCCAGCAGCTCACCGCCGGACTGGTCCGCGGCCTGCCGTTCGCGCTCACCAGCGCGCAGCAGCGCGCGGTCGGCGA
GATCGCCGCCGACCTCGGCGCCGCCCATCCGATGCAGCGCCTGCTGCAGGGCGACGTCGGCAGCGGCAAGACCATCGTCG
CCGCGCTCGCCATGCTGCAGGCCGCCGAGAACGGCTGGCAAGCGGCGCTGATGGCGCCGACCGAAATCCTTGCCGAACAG
CACTACAAGAAGCTCGCCGCCTGGCTGGAGCCGCTTGGCGTCGGCATCGCCTGGCTGTCGGGCAGCCGCAGGAAGCGCGA
GCGCGAGGCCGAACTTGCCAGGTTGCAGAGCGGCGAGGTGCTGCTCGCCGTCGGCACCCATGCGCTGATCGAGGACGAGG
TCGCACTGCCGCGCCTCGCGCTGGCGATCATCGACGAGCAGCACCGTTTCGGCGTGCGCCAGCGCCTGGCGCTGCGCGAG
AAGGGCGCGCACGGCCTGCGCCCGCACATGCTGATGATGTCGGCCACGCCGATCCCGCGCACGCTCGCGATGACCCACTA
CGCCGACCTCGACGTCTCGGTGCTCGACGAACTGCCGCCCGGGCGTACGCCGATCCGCACCAAGCTGGTGTCGGACGCGC
GCCGGGAGGAGGTCGTCGGCCGTGTGCGCGACGCCTGCCTGCAGGGCCGCCAGGCCTACTGGGTGTGCCCGCTGATCGAG
GAGTCCGAGGCGCTGCAACTGCAGACCGCGCTCGACACCTACGCCGCGCTGTCGGAGGCGCTGCCCGAACTGCGCGTGGG
CCTGGTGCACGGCCGCCTGAAGGCCGACGAGAAGTCGGCGACGATGGCCGCCTTTTCCGCCGGCGAGCTGCAGGTGCTGG
TGGCGACCACGGTGATCGAGGTCGGCGTGGATGTGCCCAATGCGAGCCTGATGGTGATCGAGCACGCCGAGCGCTTCGGC
CTCGCCCAGTTGCACCAGCTGCGCGGGCGGGTCGGGCGCGGCACCGCGGAATCGGTATGCATCCTGCTGTTCGCCCAGCC
GCTGTCGGAGAACGGGCGCGCGCGGCTGAAGGTCATCTATGAACACACCGACGGCTTCGCGATCGCGCGTGAGGATCTGC
AACTGCGCGGCCCGGGCGAATTCGTCGGTGCCCGGCAGAGCGGCGTGCCGCTGCTGCGCTACGCCGATCTGGAACTCGAC
GCCGGGTTGATCGAACCCGCGCGCGCACTTGCCGAACGCATGCTGCGCGACGCCCCCGCCCAGGCCGATGCGCTGATGCA
GCGCTGGCTCGGTGGGCGGGAGTCGCTGCTGCGCGCCTGA

Upstream 100 bases:

>100_bases
GCCGAGCCCTATCCGGCGCGCGCCGCGGTCGGCGTCAAGGAGTTGCCCAAGGGCGCGGTGGTCGAGGCCGACGCCATCCT
GGTGCTCGGCTGAGCGCCAC

Downstream 100 bases:

>100_bases
TGGCCGAGCCGGTCGGCTAAGATCACGGCCTTGCTCCGCTTTCCCGTGTCACCGATGCAGCACCCCGCTTCCGGCGAGTC
CTGGCTCGCCCGCCCGCCGC

Product: ATP-dependent DNA helicase RecG

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 679; Mature: 679

Protein sequence:

>679_residues
MQSGWAGLGEQLASRLRKLDLHRQEDLVVHLPLRYEDETRLTPIGVARAGFPAQIEGEVTSCEVVLRPRRQLVARVRDDS
GTLVARWINFYPSQQKQLAVGRRVRLFGEVRGGFFGDEMVHPRVRNIDPGEGLPEALTPVYPTTAGLGQTTLRKLIDRAL
KTVPLQDLLPEDARRALDLPGFAEALTALHHPAPDADPIALENREHPAWRRIKFEELLVQQLSLRRAYNARRAREAVVLP
ARQQLTAGLVRGLPFALTSAQQRAVGEIAADLGAAHPMQRLLQGDVGSGKTIVAALAMLQAAENGWQAALMAPTEILAEQ
HYKKLAAWLEPLGVGIAWLSGSRRKREREAELARLQSGEVLLAVGTHALIEDEVALPRLALAIIDEQHRFGVRQRLALRE
KGAHGLRPHMLMMSATPIPRTLAMTHYADLDVSVLDELPPGRTPIRTKLVSDARREEVVGRVRDACLQGRQAYWVCPLIE
ESEALQLQTALDTYAALSEALPELRVGLVHGRLKADEKSATMAAFSAGELQVLVATTVIEVGVDVPNASLMVIEHAERFG
LAQLHQLRGRVGRGTAESVCILLFAQPLSENGRARLKVIYEHTDGFAIAREDLQLRGPGEFVGARQSGVPLLRYADLELD
AGLIEPARALAERMLRDAPAQADALMQRWLGGRESLLRA

Sequences:

>Translated_679_residues
MQSGWAGLGEQLASRLRKLDLHRQEDLVVHLPLRYEDETRLTPIGVARAGFPAQIEGEVTSCEVVLRPRRQLVARVRDDS
GTLVARWINFYPSQQKQLAVGRRVRLFGEVRGGFFGDEMVHPRVRNIDPGEGLPEALTPVYPTTAGLGQTTLRKLIDRAL
KTVPLQDLLPEDARRALDLPGFAEALTALHHPAPDADPIALENREHPAWRRIKFEELLVQQLSLRRAYNARRAREAVVLP
ARQQLTAGLVRGLPFALTSAQQRAVGEIAADLGAAHPMQRLLQGDVGSGKTIVAALAMLQAAENGWQAALMAPTEILAEQ
HYKKLAAWLEPLGVGIAWLSGSRRKREREAELARLQSGEVLLAVGTHALIEDEVALPRLALAIIDEQHRFGVRQRLALRE
KGAHGLRPHMLMMSATPIPRTLAMTHYADLDVSVLDELPPGRTPIRTKLVSDARREEVVGRVRDACLQGRQAYWVCPLIE
ESEALQLQTALDTYAALSEALPELRVGLVHGRLKADEKSATMAAFSAGELQVLVATTVIEVGVDVPNASLMVIEHAERFG
LAQLHQLRGRVGRGTAESVCILLFAQPLSENGRARLKVIYEHTDGFAIAREDLQLRGPGEFVGARQSGVPLLRYADLELD
AGLIEPARALAERMLRDAPAQADALMQRWLGGRESLLRA
>Mature_679_residues
MQSGWAGLGEQLASRLRKLDLHRQEDLVVHLPLRYEDETRLTPIGVARAGFPAQIEGEVTSCEVVLRPRRQLVARVRDDS
GTLVARWINFYPSQQKQLAVGRRVRLFGEVRGGFFGDEMVHPRVRNIDPGEGLPEALTPVYPTTAGLGQTTLRKLIDRAL
KTVPLQDLLPEDARRALDLPGFAEALTALHHPAPDADPIALENREHPAWRRIKFEELLVQQLSLRRAYNARRAREAVVLP
ARQQLTAGLVRGLPFALTSAQQRAVGEIAADLGAAHPMQRLLQGDVGSGKTIVAALAMLQAAENGWQAALMAPTEILAEQ
HYKKLAAWLEPLGVGIAWLSGSRRKREREAELARLQSGEVLLAVGTHALIEDEVALPRLALAIIDEQHRFGVRQRLALRE
KGAHGLRPHMLMMSATPIPRTLAMTHYADLDVSVLDELPPGRTPIRTKLVSDARREEVVGRVRDACLQGRQAYWVCPLIE
ESEALQLQTALDTYAALSEALPELRVGLVHGRLKADEKSATMAAFSAGELQVLVATTVIEVGVDVPNASLMVIEHAERFG
LAQLHQLRGRVGRGTAESVCILLFAQPLSENGRARLKVIYEHTDGFAIAREDLQLRGPGEFVGARQSGVPLLRYADLELD
AGLIEPARALAERMLRDAPAQADALMQRWLGGRESLLRA

Specific function: Critical role in recombination and DNA repair. Help process Holliday junction intermediates to mature products by catalyzing branch migration. Has a DNA unwinding activity characteristic of a DNA helicase with a 3' to 5' polarity. RecG unwind branched dup

COG id: COG1200

COG function: function code LK; RecG-like helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367254, Length=676, Percent_Identity=51.7751479289941, Blast_Score=635, Evalue=0.0,
Organism=Escherichia coli, GI1787357, Length=410, Percent_Identity=35.609756097561, Blast_Score=229, Evalue=4e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR004609
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C; PF01336 tRNA_anti [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 74754; Mature: 74754

Theoretical pI: Translated: 8.07; Mature: 8.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSGWAGLGEQLASRLRKLDLHRQEDLVVHLPLRYEDETRLTPIGVARAGFPAQIEGEVT
CCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCEECCCCCCCCCCCCCCCCCCEECCCCE
SCEVVLRPRRQLVARVRDDSGTLVARWINFYPSQQKQLAVGRRVRLFGEVRGGFFGDEMV
EEHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCHHC
HPRVRNIDPGEGLPEALTPVYPTTAGLGQTTLRKLIDRALKTVPLQDLLPEDARRALDLP
CCCCCCCCCCCCCCHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHCCHHHHHHCCCC
GFAEALTALHHPAPDADPIALENREHPAWRRIKFEELLVQQLSLRRAYNARRAREAVVLP
CHHHHHHHHHCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECC
ARQQLTAGLVRGLPFALTSAQQRAVGEIAADLGAAHPMQRLLQGDVGSGKTIVAALAMLQ
CHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHHHHHHHH
AAENGWQAALMAPTEILAEQHYKKLAAWLEPLGVGIAWLSGSRRKREREAELARLQSGEV
HHCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHCCCCE
LLAVGTHALIEDEVALPRLALAIIDEQHRFGVRQRLALREKGAHGLRPHMLMMSATPIPR
EEEECCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCH
TLAMTHYADLDVSVLDELPPGRTPIRTKLVSDARREEVVGRVRDACLQGRQAYWVCPLIE
HHHHHHHCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCC
ESEALQLQTALDTYAALSEALPELRVGLVHGRLKADEKSATMAAFSAGELQVLVATTVIE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHEECCCCEEEEHHHHHHH
VGVDVPNASLMVIEHAERFGLAQLHQLRGRVGRGTAESVCILLFAQPLSENGRARLKVIY
HCCCCCCCCEEEEECHHHCCHHHHHHHHHHCCCCCHHHEEEEEEECCCCCCCCEEEEEEE
EHTDGFAIAREDLQLRGPGEFVGARQSGVPLLRYADLELDAGLIEPARALAERMLRDAPA
EECCCCEEEECCCEECCCCHHCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCC
QADALMQRWLGGRESLLRA
HHHHHHHHHCCCHHHHHCC
>Mature Secondary Structure
MQSGWAGLGEQLASRLRKLDLHRQEDLVVHLPLRYEDETRLTPIGVARAGFPAQIEGEVT
CCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCEECCCCCCCCCCCCCCCCCCEECCCCE
SCEVVLRPRRQLVARVRDDSGTLVARWINFYPSQQKQLAVGRRVRLFGEVRGGFFGDEMV
EEHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCHHC
HPRVRNIDPGEGLPEALTPVYPTTAGLGQTTLRKLIDRALKTVPLQDLLPEDARRALDLP
CCCCCCCCCCCCCCHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHCCHHHHHHCCCC
GFAEALTALHHPAPDADPIALENREHPAWRRIKFEELLVQQLSLRRAYNARRAREAVVLP
CHHHHHHHHHCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECC
ARQQLTAGLVRGLPFALTSAQQRAVGEIAADLGAAHPMQRLLQGDVGSGKTIVAALAMLQ
CHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHHHHHHHH
AAENGWQAALMAPTEILAEQHYKKLAAWLEPLGVGIAWLSGSRRKREREAELARLQSGEV
HHCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHCCCCE
LLAVGTHALIEDEVALPRLALAIIDEQHRFGVRQRLALREKGAHGLRPHMLMMSATPIPR
EEEECCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCH
TLAMTHYADLDVSVLDELPPGRTPIRTKLVSDARREEVVGRVRDACLQGRQAYWVCPLIE
HHHHHHHCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCC
ESEALQLQTALDTYAALSEALPELRVGLVHGRLKADEKSATMAAFSAGELQVLVATTVIE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHEECCCCEEEEHHHHHHH
VGVDVPNASLMVIEHAERFGLAQLHQLRGRVGRGTAESVCILLFAQPLSENGRARLKVIY
HCCCCCCCCEEEEECHHHCCHHHHHHHHHHCCCCCHHHEEEEEEECCCCCCCCEEEEEEE
EHTDGFAIAREDLQLRGPGEFVGARQSGVPLLRYADLELDAGLIEPARALAERMLRDAPA
EECCCCEEEECCCEECCCCHHCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCC
QADALMQRWLGGRESLLRA
HHHHHHHHHCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]