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The map label for this gene is aspA [H]

Identifier: 119896728

GI number: 119896728

Start: 462427

End: 463827

Strand: Reverse

Name: aspA [H]

Synonym: azo0437

Alternate gene names: 119896728

Gene position: 463827-462427 (Counterclockwise)

Preceding gene: 119896729

Following gene: 119896727

Centisome position: 10.6

GC content: 65.74

Gene sequence:

>1401_bases
ATGCAAACCACCCGCATCGAGCACGATCTGCTCGGCGAACGCGAAATCCCGGCCGACGTGTATTGGGGCATCCACAGCCT
GCGCGCGCTGGAAAACTACCCGATCACCGGCAAGTCGATCGGCGCCTATCCGGAACTGGTGAAGGCGCTGGCGCTGGTGA
AGCAGGCGGCGGCACGCACCAATCACCAGCTCGGCCAGCTCGACGAGGGCCGCTTCCACGCGATCGACGAGGCCTGCCGC
GAGATCGCCGAGGGCCGGTTGCACAACCAGTTCGTCGTCGACGTGATCCAGGGCGGCGCCGGCACTTCGACCAACATGAA
CGCCAACGAGGTGATCGCCAACCTCGCGCTCGAAAAGCTCGGCTGGCCCAAGGGCCACTACAAGGAGCTGCACCCGATCA
ACCACGTGAACATGAGCCAGAGCACCAACGACGTCTATCCGACGGCGCTGAAGCTGGCGGTGGTGTTCGCGATCCAGGGC
CTGCTCGACGCGATGGAGACCCTGCGCCTGGCCTATCTCGCCAAGGCGGAGGAATTCAAGGACATCCTCAAGATCGGCCG
CACCCAGTTGCAGGACGCGGTGCCGATGACGCTGGGCCAGGAGTTCTCCACCTATGCGGTGATGATGAAGGAGGATGTCG
CCCGCCTGCGCGAGGCGATCTGCCTGATCCAGGAGATCAACCTCGGCGCCACCGCGATCGGCACCGGCATCAACACCGAC
ATCCGCTACGCCAAGATGGCGACCGAACACCTCGCCGAACTGTCCGGGGTGAAGCTGGTGCCGGCCGAGAACCTGATCGA
GGCGACGCAGGACACCGGCGCCTTCGTGCAGCTCTCCGGCGTGCTGAAGCGCATCGCCTGCAAGCTCTCCAAGGTGTGCA
ACGACCTGCGCCTGCTCTCCTCCGGCCCGCAGGCCGGCTTCAACGAGATCAACCTGCCCGAGCGCGCGGCGGGGTCGTCC
ATCATGCCGGGCAAGGTGAATCCGATCATCCCGGAAGTGGTGAACCAGATCGCGTTCGAGGTGATCGGCAACGACGTCAC
GATCACGCTGGCGTCCGAGGGCGGCCAGTTGCAACTCAATGCCTTCGAGCCCATCATCGGCCACTCCCTGTTCAAGAGCA
TCGAGCACCTGGAAGCCGGCTGCCGCACGCTCACCGACAACTGCGTACGCGGCATCACCGCCAACCGCGAGCTGCTCGCG
GAGCGGGTGCGCACCTCGGCCGGGCTGGCGACCGCGCTCAATCCGCACATCGGTTACGAAAACGCCACCTGGGCTGCGCG
CGAGGCGCTAAAGACCGGGCGCAGCGTGGCCGAACTGGTGCTGGAGCGCGGGCTGATGGACAAGGAGAGCCTGGAACGCA
CGCTGCGGCCGGAAGTGCTGACCGCGCCGCGCGCCGAATAA

Upstream 100 bases:

>100_bases
CCGTCACACCGCTGCGCGCGGACGCGCCGCACCCTCTGCGCCGCGCGCGGTCGAACCCGACGAGCGGCGCATCCGACGCC
GCACACCACGAGGAGACACC

Downstream 100 bases:

>100_bases
GCCGCCACCTCACCCGGAGCCCGTCCTCTTGCCACGCCAGCCCGTCATCGCCCTCGTCGCCACCGGCGGCACCATCGCAG
GTACCGCTGACACCCCGGGG

Product: aspartate ammonia-lyase

Products: NA

Alternate protein names: Aspartase [H]

Number of amino acids: Translated: 466; Mature: 466

Protein sequence:

>466_residues
MQTTRIEHDLLGEREIPADVYWGIHSLRALENYPITGKSIGAYPELVKALALVKQAAARTNHQLGQLDEGRFHAIDEACR
EIAEGRLHNQFVVDVIQGGAGTSTNMNANEVIANLALEKLGWPKGHYKELHPINHVNMSQSTNDVYPTALKLAVVFAIQG
LLDAMETLRLAYLAKAEEFKDILKIGRTQLQDAVPMTLGQEFSTYAVMMKEDVARLREAICLIQEINLGATAIGTGINTD
IRYAKMATEHLAELSGVKLVPAENLIEATQDTGAFVQLSGVLKRIACKLSKVCNDLRLLSSGPQAGFNEINLPERAAGSS
IMPGKVNPIIPEVVNQIAFEVIGNDVTITLASEGGQLQLNAFEPIIGHSLFKSIEHLEAGCRTLTDNCVRGITANRELLA
ERVRTSAGLATALNPHIGYENATWAAREALKTGRSVAELVLERGLMDKESLERTLRPEVLTAPRAE

Sequences:

>Translated_466_residues
MQTTRIEHDLLGEREIPADVYWGIHSLRALENYPITGKSIGAYPELVKALALVKQAAARTNHQLGQLDEGRFHAIDEACR
EIAEGRLHNQFVVDVIQGGAGTSTNMNANEVIANLALEKLGWPKGHYKELHPINHVNMSQSTNDVYPTALKLAVVFAIQG
LLDAMETLRLAYLAKAEEFKDILKIGRTQLQDAVPMTLGQEFSTYAVMMKEDVARLREAICLIQEINLGATAIGTGINTD
IRYAKMATEHLAELSGVKLVPAENLIEATQDTGAFVQLSGVLKRIACKLSKVCNDLRLLSSGPQAGFNEINLPERAAGSS
IMPGKVNPIIPEVVNQIAFEVIGNDVTITLASEGGQLQLNAFEPIIGHSLFKSIEHLEAGCRTLTDNCVRGITANRELLA
ERVRTSAGLATALNPHIGYENATWAAREALKTGRSVAELVLERGLMDKESLERTLRPEVLTAPRAE
>Mature_466_residues
MQTTRIEHDLLGEREIPADVYWGIHSLRALENYPITGKSIGAYPELVKALALVKQAAARTNHQLGQLDEGRFHAIDEACR
EIAEGRLHNQFVVDVIQGGAGTSTNMNANEVIANLALEKLGWPKGHYKELHPINHVNMSQSTNDVYPTALKLAVVFAIQG
LLDAMETLRLAYLAKAEEFKDILKIGRTQLQDAVPMTLGQEFSTYAVMMKEDVARLREAICLIQEINLGATAIGTGINTD
IRYAKMATEHLAELSGVKLVPAENLIEATQDTGAFVQLSGVLKRIACKLSKVCNDLRLLSSGPQAGFNEINLPERAAGSS
IMPGKVNPIIPEVVNQIAFEVIGNDVTITLASEGGQLQLNAFEPIIGHSLFKSIEHLEAGCRTLTDNCVRGITANRELLA
ERVRTSAGLATALNPHIGYENATWAAREALKTGRSVAELVLERGLMDKESLERTLRPEVLTAPRAE

Specific function: Unknown

COG id: COG1027

COG function: function code E; Aspartate ammonia-lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family. Aspartase subfamily [H]

Homologues:

Organism=Homo sapiens, GI19743875, Length=461, Percent_Identity=41.8655097613883, Blast_Score=357, Evalue=1e-98,
Organism=Escherichia coli, GI87082375, Length=460, Percent_Identity=53.4782608695652, Blast_Score=486, Evalue=1e-138,
Organism=Escherichia coli, GI1787896, Length=459, Percent_Identity=40.0871459694989, Blast_Score=344, Evalue=8e-96,
Organism=Caenorhabditis elegans, GI17553882, Length=465, Percent_Identity=41.2903225806452, Blast_Score=369, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI32565146, Length=328, Percent_Identity=41.7682926829268, Blast_Score=273, Evalue=2e-73,
Organism=Saccharomyces cerevisiae, GI6324993, Length=461, Percent_Identity=40.7809110629067, Blast_Score=349, Evalue=5e-97,
Organism=Drosophila melanogaster, GI24640179, Length=465, Percent_Identity=42.3655913978495, Blast_Score=371, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24640177, Length=465, Percent_Identity=42.3655913978495, Blast_Score=370, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24662684, Length=463, Percent_Identity=41.6846652267819, Blast_Score=357, Evalue=8e-99,
Organism=Drosophila melanogaster, GI78710009, Length=463, Percent_Identity=39.7408207343413, Blast_Score=343, Evalue=1e-94,
Organism=Drosophila melanogaster, GI24583245, Length=463, Percent_Identity=37.3650107991361, Blast_Score=323, Evalue=2e-88,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004708
- InterPro:   IPR018951
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761 [H]

Pfam domain/function: PF10415 FumaraseC_C; PF00206 Lyase_1 [H]

EC number: =4.3.1.1 [H]

Molecular weight: Translated: 50804; Mature: 50804

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTTRIEHDLLGEREIPADVYWGIHSLRALENYPITGKSIGAYPELVKALALVKQAAART
CCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
NHQLGQLDEGRFHAIDEACREIAEGRLHNQFVVDVIQGGAGTSTNMNANEVIANLALEKL
CCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHC
GWPKGHYKELHPINHVNMSQSTNDVYPTALKLAVVFAIQGLLDAMETLRLAYLAKAEEFK
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DILKIGRTQLQDAVPMTLGQEFSTYAVMMKEDVARLREAICLIQEINLGATAIGTGINTD
HHHHHHHHHHHHHCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCC
IRYAKMATEHLAELSGVKLVPAENLIEATQDTGAFVQLSGVLKRIACKLSKVCNDLRLLS
HHHHHHHHHHHHHHCCCEECCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
SGPQAGFNEINLPERAAGSSIMPGKVNPIIPEVVNQIAFEVIGNDVTITLASEGGQLQLN
CCCCCCCCCCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCEEEEE
AFEPIIGHSLFKSIEHLEAGCRTLTDNCVRGITANRELLAERVRTSAGLATALNPHIGYE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHCCCCCCCC
NATWAAREALKTGRSVAELVLERGLMDKESLERTLRPEVLTAPRAE
CCHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHCCCHHCCCCCCC
>Mature Secondary Structure
MQTTRIEHDLLGEREIPADVYWGIHSLRALENYPITGKSIGAYPELVKALALVKQAAART
CCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
NHQLGQLDEGRFHAIDEACREIAEGRLHNQFVVDVIQGGAGTSTNMNANEVIANLALEKL
CCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHC
GWPKGHYKELHPINHVNMSQSTNDVYPTALKLAVVFAIQGLLDAMETLRLAYLAKAEEFK
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DILKIGRTQLQDAVPMTLGQEFSTYAVMMKEDVARLREAICLIQEINLGATAIGTGINTD
HHHHHHHHHHHHHCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCC
IRYAKMATEHLAELSGVKLVPAENLIEATQDTGAFVQLSGVLKRIACKLSKVCNDLRLLS
HHHHHHHHHHHHHHCCCEECCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
SGPQAGFNEINLPERAAGSSIMPGKVNPIIPEVVNQIAFEVIGNDVTITLASEGGQLQLN
CCCCCCCCCCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCEEEEE
AFEPIIGHSLFKSIEHLEAGCRTLTDNCVRGITANRELLAERVRTSAGLATALNPHIGYE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHCCCCCCCC
NATWAAREALKTGRSVAELVLERGLMDKESLERTLRPEVLTAPRAE
CCHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3096982 [H]