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The map label for this gene is ansB [H]

Identifier: 119896727

GI number: 119896727

Start: 461424

End: 462398

Strand: Reverse

Name: ansB [H]

Synonym: azo0436

Alternate gene names: 119896727

Gene position: 462398-461424 (Counterclockwise)

Preceding gene: 119896728

Following gene: 119896724

Centisome position: 10.57

GC content: 75.18

Gene sequence:

>975_bases
TTGCCACGCCAGCCCGTCATCGCCCTCGTCGCCACCGGCGGCACCATCGCAGGTACCGCTGACACCCCGGGGGCGGTCAC
GGGCTATACCGCCGGCGTGCTCGGCGCCGAGGCCTTGCTCGCGGCCTTCCCCGGGCTGGGCGAGATCGCCGCGCTGCGGG
CCGAGACCCTGTTCAGCCTGGACAGCAAGGACATGGCGCCGGCGCACTGGCTGGCGCTGGCACAACGGGTGCAGGCGCTG
GCCGACGACCCGGCGGTCGACGGCGTGGTGATCACGCACGGCACGGACACGCTGGAGGAATCGGCGTTCTTCGTGCATCT
GACGGTGGCCACCGCCAAGCCGGTGGTGTTTACCGCGGCGATGCGACCGGCGACCGCGCTGTCGGCCGACGGGCCGATGA
ACCTGCTCGCGGCGGTGCGGGTTGCGGCGGCGCCCGCCTTCCACGGCCTCGGCACGCTGGTGGCGATCAACGACCGGGTC
TATGCCGCGCGCGACGTGCAGAAGCAGCGCACCCACGGCCTGGATGCGTTTGGCGCGGGCGAAGGCGCCGCGGTGGGACG
CAGCGACCCGGCGCGGCCCGGCCTGCTGGTGAATCGCGATGGCGGCAGTTGCGCGGCGGCGCTGGCCAGCGCTGCGCTAC
CCGCGGTCGAGGTGCTTTACGTTGCCGCCGGGGCATCGCCCGCGCTGCTCGACGCCGTGCGCGGCAGCGGTGCGGCCGGC
GCGGTGCTCGCCTTTCCCGGCAACGGCAGCGTGCCCGAGGCCTGGAAGCCGGCGATCGACGCGGCGCTGCACGCCGGCAT
GGTGCTGGTGCGCGCCAGCCGGGTGCCCGCCGGGCCGGTGGGCCACGACACCCGCCTGCCCGCGTCGTTGCTCAGCGCGG
GCGACCTGCCGCCATCGAAGGCGCGGGTGGCGCTGATGCTGGCGCTGGCCTGCGACCGCCCGCAGCGCTTCTGCACCCTC
GCCGCCCCGCACTGA

Upstream 100 bases:

>100_bases
GGGCTGATGGACAAGGAGAGCCTGGAACGCACGCTGCGGCCGGAAGTGCTGACCGCGCCGCGCGCCGAATAAGCCGCCAC
CTCACCCGGAGCCCGTCCTC

Downstream 100 bases:

>100_bases
CACGCCGCTTCAGGCCCCCCAGCGCCGGCGCAGCGCGAACAGCGCCAGCAGACCGCCGCCGATCAGCGCTAGCGCACCCG
GTTCGGGGATGACCTGGCAC

Product: L-asparaginase II

Products: NA

Alternate protein names: L-ASNase; L-asparagine amidohydrolase [H]

Number of amino acids: Translated: 324; Mature: 323

Protein sequence:

>324_residues
MPRQPVIALVATGGTIAGTADTPGAVTGYTAGVLGAEALLAAFPGLGEIAALRAETLFSLDSKDMAPAHWLALAQRVQAL
ADDPAVDGVVITHGTDTLEESAFFVHLTVATAKPVVFTAAMRPATALSADGPMNLLAAVRVAAAPAFHGLGTLVAINDRV
YAARDVQKQRTHGLDAFGAGEGAAVGRSDPARPGLLVNRDGGSCAAALASAALPAVEVLYVAAGASPALLDAVRGSGAAG
AVLAFPGNGSVPEAWKPAIDAALHAGMVLVRASRVPAGPVGHDTRLPASLLSAGDLPPSKARVALMLALACDRPQRFCTL
AAPH

Sequences:

>Translated_324_residues
MPRQPVIALVATGGTIAGTADTPGAVTGYTAGVLGAEALLAAFPGLGEIAALRAETLFSLDSKDMAPAHWLALAQRVQAL
ADDPAVDGVVITHGTDTLEESAFFVHLTVATAKPVVFTAAMRPATALSADGPMNLLAAVRVAAAPAFHGLGTLVAINDRV
YAARDVQKQRTHGLDAFGAGEGAAVGRSDPARPGLLVNRDGGSCAAALASAALPAVEVLYVAAGASPALLDAVRGSGAAG
AVLAFPGNGSVPEAWKPAIDAALHAGMVLVRASRVPAGPVGHDTRLPASLLSAGDLPPSKARVALMLALACDRPQRFCTL
AAPH
>Mature_323_residues
PRQPVIALVATGGTIAGTADTPGAVTGYTAGVLGAEALLAAFPGLGEIAALRAETLFSLDSKDMAPAHWLALAQRVQALA
DDPAVDGVVITHGTDTLEESAFFVHLTVATAKPVVFTAAMRPATALSADGPMNLLAAVRVAAAPAFHGLGTLVAINDRVY
AARDVQKQRTHGLDAFGAGEGAAVGRSDPARPGLLVNRDGGSCAAALASAALPAVEVLYVAAGASPALLDAVRGSGAAGA
VLAFPGNGSVPEAWKPAIDAALHAGMVLVRASRVPAGPVGHDTRLPASLLSAGDLPPSKARVALMLALACDRPQRFCTLA
APH

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1789327, Length=301, Percent_Identity=37.8737541528239, Blast_Score=171, Evalue=6e-44,
Organism=Escherichia coli, GI1788065, Length=182, Percent_Identity=31.8681318681319, Blast_Score=74, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6323189, Length=321, Percent_Identity=35.8255451713396, Blast_Score=154, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6323187, Length=321, Percent_Identity=35.8255451713396, Blast_Score=154, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6323186, Length=321, Percent_Identity=35.8255451713396, Blast_Score=154, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6323184, Length=321, Percent_Identity=35.8255451713396, Blast_Score=154, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6320527, Length=325, Percent_Identity=32.9230769230769, Blast_Score=150, Evalue=2e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004550
- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 32404; Mature: 32273

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: PS00144 ASN_GLN_ASE_1 ; PS00917 ASN_GLN_ASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRQPVIALVATGGTIAGTADTPGAVTGYTAGVLGAEALLAAFPGLGEIAALRAETLFSL
CCCCCEEEEEECCCEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCC
DSKDMAPAHWLALAQRVQALADDPAVDGVVITHGTDTLEESAFFVHLTVATAKPVVFTAA
CCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHCCEEEEEEEEECCCCEEEEEC
MRPATALSADGPMNLLAAVRVAAAPAFHGLGTLVAINDRVYAARDVQKQRTHGLDAFGAG
CCCCCCCCCCCCHHHHHHHHHHHCHHHHCCEEEEEECCCEEEHHHHHHHHHCCCCCCCCC
EGAAVGRSDPARPGLLVNRDGGSCAAALASAALPAVEVLYVAAGASPALLDAVRGSGAAG
CCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHCCCCCCC
AVLAFPGNGSVPEAWKPAIDAALHAGMVLVRASRVPAGPVGHDTRLPASLLSAGDLPPSK
EEEEECCCCCCCCHHHHHHHHHHHHCEEEEEECCCCCCCCCCCCCCCHHHHHCCCCCCCH
ARVALMLALACDRPQRFCTLAAPH
HHHHHHHHHHCCCCHHEEEECCCC
>Mature Secondary Structure 
PRQPVIALVATGGTIAGTADTPGAVTGYTAGVLGAEALLAAFPGLGEIAALRAETLFSL
CCCCEEEEEECCCEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCC
DSKDMAPAHWLALAQRVQALADDPAVDGVVITHGTDTLEESAFFVHLTVATAKPVVFTAA
CCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHCCEEEEEEEEECCCCEEEEEC
MRPATALSADGPMNLLAAVRVAAAPAFHGLGTLVAINDRVYAARDVQKQRTHGLDAFGAG
CCCCCCCCCCCCHHHHHHHHHHHCHHHHCCEEEEEECCCEEEHHHHHHHHHCCCCCCCCC
EGAAVGRSDPARPGLLVNRDGGSCAAALASAALPAVEVLYVAAGASPALLDAVRGSGAAG
CCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHCCCCCCC
AVLAFPGNGSVPEAWKPAIDAALHAGMVLVRASRVPAGPVGHDTRLPASLLSAGDLPPSK
EEEEECCCCCCCCHHHHHHHHHHHHCEEEEEECCCCCCCCCCCCCCCHHHHHCCCCCCCH
ARVALMLALACDRPQRFCTLAAPH
HHHHHHHHHHCCCCHHEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3026924; 3194219; 3379033; 8348975 [H]