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The map label for this gene is cysH [H]

Identifier: 119896721

GI number: 119896721

Start: 455534

End: 456304

Strand: Reverse

Name: cysH [H]

Synonym: azo0430

Alternate gene names: 119896721

Gene position: 456304-455534 (Counterclockwise)

Preceding gene: 119896722

Following gene: 119896720

Centisome position: 10.43

GC content: 67.44

Gene sequence:

>771_bases
GTGAGCGGCGCGGTGTCGATGCTGCGTCCGGCAGCCACGAATCCGGCCACCCGCCCCGAGCTGACCGAGGCGCTGCGCGC
CAGCGTCAGCGCCAAGGCCGCGGCCGCGCGCGAGTTGCTGGCCGCCGCGGTGGCCGAACTCGGTGCCAATGGCGAGATCA
CCTTCGCCAACAGCTTCGGCGCCGAGGACATGGTGCTGACCGACCTGATCCTGGGCGGCAAGCTGCCGATCGAGATCTTC
TCGCTCGACACCGGCCGCCTGCCGGCCGAAACCTATACGCTGATGGGCGCAGTCGAGCAGCGTTACGGCACGAAGCTGAA
GGTGTTCTTCCCGGACGCGACCGCGGTCGAGCAGTTCGTCCGCAGCGAGGGCATCAATGCGTTCTACGATTCGGTGGAGC
TGCGCAAGGCCTGTTGCGGCATCCGCAAGATGGAGCCGTTGCGCCGCGCGCTGGCCGGCAAGAAGGCGTGGGTGACCGGG
CTGCGCGCCGCGCAGTCGGTGACGCGTACGGGCCTTGCGCTGCGCGAATTCGACACCGCCAACGGGCTGGAGAAATTCAA
CCCGCTGTCCGACTGGACGGAGGCCGAGGTATGGGCGTATATCCGGATCAACGAGGTGCCCTACAACGCGCTGCACGACC
AGTTCTATCCGAGCATCGGCTGCGCACCCTGTACGCGCGCGATCGCGCTGGGCGAGGACATCCGCGCCGGCCGCTGGTGG
TGGGAAGATCCCGCTTCCAAGGAATGCGGTCTGCACGTCAAGAAGACTTGA

Upstream 100 bases:

>100_bases
AAGCGGCGCTCGCCAGCCTCGGCGACTTCAGCGTGCCCTACCAGGGCGCGGTGGACGAGCCGCGCCCGCTGTGGGCACGT
GCCCAACGCGGAGTGGCGGC

Downstream 100 bases:

>100_bases
GCATGAAGACCGCGAAACGCGTAGCGCCCTCGTTCCTGCACCGCCTGCACGCGCTGCAGGCCGGGCTGCGCGCCGCGTTG
CGGGGCTTGCGTGCCCACTG

Product: phosphoadenosine phosphosulfate reductase

Products: NA

Alternate protein names: 3'-phosphoadenylylsulfate reductase; PAPS reductase, thioredoxin dependent; PAPS sulfotransferase; PAdoPS reductase [H]

Number of amino acids: Translated: 256; Mature: 255

Protein sequence:

>256_residues
MSGAVSMLRPAATNPATRPELTEALRASVSAKAAAARELLAAAVAELGANGEITFANSFGAEDMVLTDLILGGKLPIEIF
SLDTGRLPAETYTLMGAVEQRYGTKLKVFFPDATAVEQFVRSEGINAFYDSVELRKACCGIRKMEPLRRALAGKKAWVTG
LRAAQSVTRTGLALREFDTANGLEKFNPLSDWTEAEVWAYIRINEVPYNALHDQFYPSIGCAPCTRAIALGEDIRAGRWW
WEDPASKECGLHVKKT

Sequences:

>Translated_256_residues
MSGAVSMLRPAATNPATRPELTEALRASVSAKAAAARELLAAAVAELGANGEITFANSFGAEDMVLTDLILGGKLPIEIF
SLDTGRLPAETYTLMGAVEQRYGTKLKVFFPDATAVEQFVRSEGINAFYDSVELRKACCGIRKMEPLRRALAGKKAWVTG
LRAAQSVTRTGLALREFDTANGLEKFNPLSDWTEAEVWAYIRINEVPYNALHDQFYPSIGCAPCTRAIALGEDIRAGRWW
WEDPASKECGLHVKKT
>Mature_255_residues
SGAVSMLRPAATNPATRPELTEALRASVSAKAAAARELLAAAVAELGANGEITFANSFGAEDMVLTDLILGGKLPIEIFS
LDTGRLPAETYTLMGAVEQRYGTKLKVFFPDATAVEQFVRSEGINAFYDSVELRKACCGIRKMEPLRRALAGKKAWVTGL
RAAQSVTRTGLALREFDTANGLEKFNPLSDWTEAEVWAYIRINEVPYNALHDQFYPSIGCAPCTRAIALGEDIRAGRWWW
EDPASKECGLHVKKT

Specific function: Reduction of activated sulfate into sulfite [H]

COG id: COG0175

COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PAPS reductase family. CysH subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789121, Length=212, Percent_Identity=28.7735849056604, Blast_Score=75, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6325425, Length=204, Percent_Identity=33.8235294117647, Blast_Score=95, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011798
- InterPro:   IPR004511
- InterPro:   IPR002500
- InterPro:   IPR014729 [H]

Pfam domain/function: PF01507 PAPS_reduct [H]

EC number: =1.8.4.8 [H]

Molecular weight: Translated: 27916; Mature: 27785

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGAVSMLRPAATNPATRPELTEALRASVSAKAAAARELLAAAVAELGANGEITFANSFG
CCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCC
AEDMVLTDLILGGKLPIEIFSLDTGRLPAETYTLMGAVEQRYGTKLKVFFPDATAVEQFV
CCHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCEEEEEECCHHHHHHHH
RSEGINAFYDSVELRKACCGIRKMEPLRRALAGKKAWVTGLRAAQSVTRTGLALREFDTA
HHCCCHHHHHHHHHHHHHHCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHCCC
NGLEKFNPLSDWTEAEVWAYIRINEVPYNALHDQFYPSIGCAPCTRAIALGEDIRAGRWW
CCHHHCCCCCCCCCCEEEEEEEEECCCCHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCC
WEDPASKECGLHVKKT
CCCCCCCCCCCEEECC
>Mature Secondary Structure 
SGAVSMLRPAATNPATRPELTEALRASVSAKAAAARELLAAAVAELGANGEITFANSFG
CCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCC
AEDMVLTDLILGGKLPIEIFSLDTGRLPAETYTLMGAVEQRYGTKLKVFFPDATAVEQFV
CCHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCEEEEEECCHHHHHHHH
RSEGINAFYDSVELRKACCGIRKMEPLRRALAGKKAWVTGLRAAQSVTRTGLALREFDTA
HHCCCHHHHHHHHHHHHHHCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHCCC
NGLEKFNPLSDWTEAEVWAYIRINEVPYNALHDQFYPSIGCAPCTRAIALGEDIRAGRWW
CCHHHCCCCCCCCCCEEEEEEEEECCCCHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCC
WEDPASKECGLHVKKT
CCCCCCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10710307 [H]