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The map label for this gene is cysD [H]

Identifier: 119896720

GI number: 119896720

Start: 454492

End: 455406

Strand: Reverse

Name: cysD [H]

Synonym: azo0429

Alternate gene names: 119896720

Gene position: 455406-454492 (Counterclockwise)

Preceding gene: 119896721

Following gene: 119896719

Centisome position: 10.41

GC content: 65.14

Gene sequence:

>915_bases
ATGTCGACGCTGACCAAACAGACGCTTTCCCACCTGGACTGGCTCGAAGCCGAGGCCATCCACATCCTGCGCGAGGTCGC
GGGCCAGTGTTCCAATCCGGCGCTGCTGTTCTCCGGCGGCAAGGATTCGCTGTGTCTGCTGCGGCTGGCGGAGAAGGCCT
TCCGCCCGGGCCGCTTCCCCTTCCCGCTGCTGCATGTGGATACGGGCCACAACTATCCGGAAGTGATCGAGTTCCGCGAC
CGCCGCGCCGCCGAGCTGGGCGAGCGCCTGATCGTGCGTTCGGTGGAGGATTCGATGGCACGTGGCACCGTGGTGCTGAA
GTCGCCGGATGAGTCGCGCAACAAGCACCAGTCGGTGACGCTGCTGGAGGCCATTGAAGAGTTCGGCTTCGACGCGTGTA
TCGGCGGTGCCCGTCGCGACGAGGAGAAGGCGCGGGCGAAGGAGCGGATCATGAGTTTCCGCGACGAGTTCGGCCAGTGG
GACCCGAAGAACCAGCGCCCGGAGCTGTGGAACCTGTACAACGCGCGCTCGCACAAGGGCGAGAACATCCGCGCCTTCCC
GATTTCGAACTGGACCGAGCTGGACGTGTGGCAATACATCGAGCGTGAGAAGCTCGAACTGCCGTCGATCTACTTCGCCC
ACGTGCGGCCGGTGGTGCGCAAGAACGGCCTGCTGCAGCCGGTGACCGCGCTGACCCCGGCCAAGCCGGGCGACGTCATC
GAGGACGTGCTGGTGCGCTTCCGCACGGTCGGCGACATCACCTGCACAGCGCCGGTGGAATCCGACGCGGATACCGTGGC
CAAGATTCTCGCCGAAACCGCAACCACCACGATCACCGAACGCGGAGCGACGCGGATGGACGACCAGACCTCCGAGGCGT
CGATGGAGCAGCGGAAGAAGGAAGGGTATTTCTGA

Upstream 100 bases:

>100_bases
CCTCGTTCCTGCACCGCCTGCACGCGCTGCAGGCCGGGCTGCGCGCCGCGTTGCGGGGCTTGCGTGCCCACTGAAACACA
CTCGAAACGGTAACCTGAAG

Downstream 100 bases:

>100_bases
TTTTTTGGGTGGGTCGGTTGTTTGTGGGTTGAAAACCGGGGATTCGGATTGGGCGTTGCGCGGGGTATTTGTTCCGGGGG
GCTGCGGAACTCGCCCTTCG

Product: sulfate adenylyltransferase subunit 2

Products: NA

Alternate protein names: ATP-sulfurylase small subunit; Sulfate adenylate transferase; SAT [H]

Number of amino acids: Translated: 304; Mature: 303

Protein sequence:

>304_residues
MSTLTKQTLSHLDWLEAEAIHILREVAGQCSNPALLFSGGKDSLCLLRLAEKAFRPGRFPFPLLHVDTGHNYPEVIEFRD
RRAAELGERLIVRSVEDSMARGTVVLKSPDESRNKHQSVTLLEAIEEFGFDACIGGARRDEEKARAKERIMSFRDEFGQW
DPKNQRPELWNLYNARSHKGENIRAFPISNWTELDVWQYIEREKLELPSIYFAHVRPVVRKNGLLQPVTALTPAKPGDVI
EDVLVRFRTVGDITCTAPVESDADTVAKILAETATTTITERGATRMDDQTSEASMEQRKKEGYF

Sequences:

>Translated_304_residues
MSTLTKQTLSHLDWLEAEAIHILREVAGQCSNPALLFSGGKDSLCLLRLAEKAFRPGRFPFPLLHVDTGHNYPEVIEFRD
RRAAELGERLIVRSVEDSMARGTVVLKSPDESRNKHQSVTLLEAIEEFGFDACIGGARRDEEKARAKERIMSFRDEFGQW
DPKNQRPELWNLYNARSHKGENIRAFPISNWTELDVWQYIEREKLELPSIYFAHVRPVVRKNGLLQPVTALTPAKPGDVI
EDVLVRFRTVGDITCTAPVESDADTVAKILAETATTTITERGATRMDDQTSEASMEQRKKEGYF
>Mature_303_residues
STLTKQTLSHLDWLEAEAIHILREVAGQCSNPALLFSGGKDSLCLLRLAEKAFRPGRFPFPLLHVDTGHNYPEVIEFRDR
RAAELGERLIVRSVEDSMARGTVVLKSPDESRNKHQSVTLLEAIEEFGFDACIGGARRDEEKARAKERIMSFRDEFGQWD
PKNQRPELWNLYNARSHKGENIRAFPISNWTELDVWQYIEREKLELPSIYFAHVRPVVRKNGLLQPVTALTPAKPGDVIE
DVLVRFRTVGDITCTAPVESDADTVAKILAETATTTITERGATRMDDQTSEASMEQRKKEGYF

Specific function: First step in the sulfate activation pathway. This reaction occurs early in the reductive branch of the cysteine biosynthetic pathway. [C]

COG id: COG0175

COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PAPS reductase family. CysD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789109, Length=298, Percent_Identity=49.3288590604027, Blast_Score=288, Evalue=4e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002500
- InterPro:   IPR014729
- InterPro:   IPR011784 [H]

Pfam domain/function: PF01507 PAPS_reduct [H]

EC number: =2.7.7.4 [H]

Molecular weight: Translated: 34498; Mature: 34367

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTLTKQTLSHLDWLEAEAIHILREVAGQCSNPALLFSGGKDSLCLLRLAEKAFRPGRFP
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCC
FPLLHVDTGHNYPEVIEFRDRRAAELGERLIVRSVEDSMARGTVVLKSPDESRNKHQSVT
CCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCHHHHHHHHHH
LLEAIEEFGFDACIGGARRDEEKARAKERIMSFRDEFGQWDPKNQRPELWNLYNARSHKG
HHHHHHHHCCHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCC
ENIRAFPISNWTELDVWQYIEREKLELPSIYFAHVRPVVRKNGLLQPVTALTPAKPGDVI
CCEEEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHCCCCCCHHHH
EDVLVRFRTVGDITCTAPVESDADTVAKILAETATTTITERGATRMDDQTSEASMEQRKK
HHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
EGYF
CCCC
>Mature Secondary Structure 
STLTKQTLSHLDWLEAEAIHILREVAGQCSNPALLFSGGKDSLCLLRLAEKAFRPGRFP
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCC
FPLLHVDTGHNYPEVIEFRDRRAAELGERLIVRSVEDSMARGTVVLKSPDESRNKHQSVT
CCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCHHHHHHHHHH
LLEAIEEFGFDACIGGARRDEEKARAKERIMSFRDEFGQWDPKNQRPELWNLYNARSHKG
HHHHHHHHCCHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCC
ENIRAFPISNWTELDVWQYIEREKLELPSIYFAHVRPVVRKNGLLQPVTALTPAKPGDVI
CCEEEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHCCCCCCHHHH
EDVLVRFRTVGDITCTAPVESDADTVAKILAETATTTITERGATRMDDQTSEASMEQRKK
HHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
EGYF
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA