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The map label for this gene is cysN/C [H]

Identifier: 119896719

GI number: 119896719

Start: 452792

End: 454072

Strand: Reverse

Name: cysN/C [H]

Synonym: azo0428

Alternate gene names: 119896719

Gene position: 454072-452792 (Counterclockwise)

Preceding gene: 119896720

Following gene: 119896718

Centisome position: 10.38

GC content: 66.04

Gene sequence:

>1281_bases
ATGTCCGCCCCTGAACAACTGCCCGAAATCGACAACGGCCTGCTGCGCTTCCTCACCTGCGGCAGCGTGGATGACGGCAA
GAGCACGCTGATCGGCCGCCTGCTGTTCGACACCAAGGCCATCCTCGCCGACACCATGAACGCCATCGAGAAGACCTCGG
CCAAGCGCGGCATGAGCGCGGTCGACCTCTCGCTGCTCACCGACGGCCTGCAGGCCGAGCGCGAGCAGGGCATCACCATC
GACGTCGCCTACCGCTACTTCTCCACCGGCACCCGCAAATACATCATCGCCGACGCGCCGGGCCACGAGCAGTACACCCG
CAACATGGTCACCGCGGCCTCCACCGCCAACCTGGCCATCATCCTGGTCGACGCGCGTCGCGGCGTGCTCACCCAGACCC
GCCGCCACTCCTACCTCGCCAGCCTGGTCGGCATTCCGCACCTGCTGGTGGCAGTGAACAAGATGGACCTGGTCGATTAC
GACCAGAAGGTGTTCGAGCAGATCCGCGCCGACTACCTCGCCTTCGCCGACAAGCTCGGCATCAAGGACGTGCGTTTCAT
CCCGATTTCCGCACTGGCCGGCGACATGATCGTCGACCGCGGCGACCGCCTCGGCTGGTACGACGGCCCGACGCTGCTCG
AAATCCTCGAAGCCGCGCCCGCTGCCCACACCGAAACCGCCGAGAACTTCCGCTTCCCGGTGCAGTTCGTGTGCCGCCCG
CAAGATTCCGCCAACCCCGAACTGCACGACTACCGCGGCTTCATGGGCCGGGTGGAGTCAGGCGAAGTCGCGGTCGGCGA
CGCCATCACGGTGCTGCCTTCCGGCGCCACCAGCAAGGTCAAGGCGATCGAACTCGGCGGCGAAAAGCTGCAGCGCGCGA
TCCACGAGCAGTCGGTGACCCTGCTGCTGGAAGACGAGATCGACATCTCGCGCGGCGACATGATCGTCAAGTCGGCCGAA
GCGCCGGAAGCGGTCAAGCAGATCGACGCCACCGTGTGCTGGCTGTCGGAGACGCCGATGTCCACCGCCCGCACCTACCT
CGTGCGCCACACCACCCGCGAGGCCAAGGCCAAGGTCGCCAAGATCGACTACCGCCTGGATGTGAACACGCTGGAGCAGG
AGCCGGCCACCAGCCTGGCGATGAACGACATCGCCCGTCTCACGCTGAAGCTGGCCCAGCCCATCGTGGCCGACGCCTAC
GCCGACAACCGTGCCACCGGCGCTTTCATCATCATCGACGAAAGCACCAACAACACCGTCGGCGCCGGCATGATCGGCTG
A

Upstream 100 bases:

>100_bases
ATGCCCCGGTGGCGCCGGCGGATTAAGCGCTTGGACCCACGCCCCGGAACAACCGATTAGCAGCACCGAATTCTCGAAAG
AAATCGAAGGAACGATGAAC

Downstream 100 bases:

>100_bases
CGATCCCGCTGCGCCCGGCGTCAAGGCCGGGCGCAGCAGTTCCCGCATCGTGACCACCTCCCCCTCAGGCAGCGACGACG
CCCGCACCCGCATCGACAAG

Product: putative sulfate adenylyltransferase subunit 1/adenylylsulfate kinase

Products: NA

Alternate protein names: ATP-sulfurylase large subunit; Sulfate adenylate transferase; SAT [H]

Number of amino acids: Translated: 426; Mature: 425

Protein sequence:

>426_residues
MSAPEQLPEIDNGLLRFLTCGSVDDGKSTLIGRLLFDTKAILADTMNAIEKTSAKRGMSAVDLSLLTDGLQAEREQGITI
DVAYRYFSTGTRKYIIADAPGHEQYTRNMVTAASTANLAIILVDARRGVLTQTRRHSYLASLVGIPHLLVAVNKMDLVDY
DQKVFEQIRADYLAFADKLGIKDVRFIPISALAGDMIVDRGDRLGWYDGPTLLEILEAAPAAHTETAENFRFPVQFVCRP
QDSANPELHDYRGFMGRVESGEVAVGDAITVLPSGATSKVKAIELGGEKLQRAIHEQSVTLLLEDEIDISRGDMIVKSAE
APEAVKQIDATVCWLSETPMSTARTYLVRHTTREAKAKVAKIDYRLDVNTLEQEPATSLAMNDIARLTLKLAQPIVADAY
ADNRATGAFIIIDESTNNTVGAGMIG

Sequences:

>Translated_426_residues
MSAPEQLPEIDNGLLRFLTCGSVDDGKSTLIGRLLFDTKAILADTMNAIEKTSAKRGMSAVDLSLLTDGLQAEREQGITI
DVAYRYFSTGTRKYIIADAPGHEQYTRNMVTAASTANLAIILVDARRGVLTQTRRHSYLASLVGIPHLLVAVNKMDLVDY
DQKVFEQIRADYLAFADKLGIKDVRFIPISALAGDMIVDRGDRLGWYDGPTLLEILEAAPAAHTETAENFRFPVQFVCRP
QDSANPELHDYRGFMGRVESGEVAVGDAITVLPSGATSKVKAIELGGEKLQRAIHEQSVTLLLEDEIDISRGDMIVKSAE
APEAVKQIDATVCWLSETPMSTARTYLVRHTTREAKAKVAKIDYRLDVNTLEQEPATSLAMNDIARLTLKLAQPIVADAY
ADNRATGAFIIIDESTNNTVGAGMIG
>Mature_425_residues
SAPEQLPEIDNGLLRFLTCGSVDDGKSTLIGRLLFDTKAILADTMNAIEKTSAKRGMSAVDLSLLTDGLQAEREQGITID
VAYRYFSTGTRKYIIADAPGHEQYTRNMVTAASTANLAIILVDARRGVLTQTRRHSYLASLVGIPHLLVAVNKMDLVDYD
QKVFEQIRADYLAFADKLGIKDVRFIPISALAGDMIVDRGDRLGWYDGPTLLEILEAAPAAHTETAENFRFPVQFVCRPQ
DSANPELHDYRGFMGRVESGEVAVGDAITVLPSGATSKVKAIELGGEKLQRAIHEQSVTLLLEDEIDISRGDMIVKSAEA
PEAVKQIDATVCWLSETPMSTARTYLVRHTTREAKAKVAKIDYRLDVNTLEQEPATSLAMNDIARLTLKLAQPIVADAYA
DNRATGAFIIIDESTNNTVGAGMIG

Specific function: May be the GTPase, regulating ATP sulfurylase activity [H]

COG id: COG2895

COG function: function code P; GTPases - Sulfate adenylate transferase subunit 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. CysN/nodQ subfamily [H]

Homologues:

Organism=Homo sapiens, GI223555963, Length=431, Percent_Identity=30.6264501160093, Blast_Score=180, Evalue=3e-45,
Organism=Homo sapiens, GI5729864, Length=431, Percent_Identity=30.6264501160093, Blast_Score=180, Evalue=3e-45,
Organism=Homo sapiens, GI4503475, Length=446, Percent_Identity=28.9237668161435, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI4503471, Length=445, Percent_Identity=28.314606741573, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI194018520, Length=297, Percent_Identity=28.956228956229, Blast_Score=146, Evalue=4e-35,
Organism=Homo sapiens, GI194097354, Length=297, Percent_Identity=28.956228956229, Blast_Score=146, Evalue=4e-35,
Organism=Homo sapiens, GI194018522, Length=293, Percent_Identity=29.6928327645051, Blast_Score=145, Evalue=6e-35,
Organism=Homo sapiens, GI46094014, Length=279, Percent_Identity=30.1075268817204, Blast_Score=145, Evalue=8e-35,
Organism=Homo sapiens, GI34147630, Length=329, Percent_Identity=28.2674772036474, Blast_Score=88, Evalue=1e-17,
Organism=Escherichia coli, GI1789108, Length=416, Percent_Identity=50.9615384615385, Blast_Score=424, Evalue=1e-120,
Organism=Escherichia coli, GI1789737, Length=139, Percent_Identity=35.2517985611511, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1790412, Length=139, Percent_Identity=35.2517985611511, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI2367247, Length=400, Percent_Identity=24, Blast_Score=74, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI32566629, Length=433, Percent_Identity=27.4826789838337, Blast_Score=167, Evalue=7e-42,
Organism=Caenorhabditis elegans, GI17552884, Length=445, Percent_Identity=28.0898876404494, Blast_Score=160, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI17569207, Length=445, Percent_Identity=28.0898876404494, Blast_Score=160, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI115532067, Length=279, Percent_Identity=32.258064516129, Blast_Score=159, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI115532065, Length=279, Percent_Identity=32.258064516129, Blast_Score=159, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI32566303, Length=438, Percent_Identity=26.4840182648402, Blast_Score=135, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI32566301, Length=136, Percent_Identity=36.7647058823529, Blast_Score=92, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI25141371, Length=276, Percent_Identity=25.7246376811594, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17556456, Length=150, Percent_Identity=35.3333333333333, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6325337, Length=448, Percent_Identity=29.6875, Blast_Score=176, Evalue=9e-45,
Organism=Saccharomyces cerevisiae, GI6319594, Length=448, Percent_Identity=29.6875, Blast_Score=176, Evalue=9e-45,
Organism=Saccharomyces cerevisiae, GI6322937, Length=328, Percent_Identity=31.4024390243902, Blast_Score=169, Evalue=6e-43,
Organism=Saccharomyces cerevisiae, GI6320377, Length=423, Percent_Identity=28.1323877068558, Blast_Score=130, Evalue=5e-31,
Organism=Saccharomyces cerevisiae, GI6324761, Length=295, Percent_Identity=30.8474576271186, Blast_Score=94, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24652838, Length=445, Percent_Identity=30.1123595505618, Blast_Score=164, Evalue=8e-41,
Organism=Drosophila melanogaster, GI17137572, Length=445, Percent_Identity=30.1123595505618, Blast_Score=164, Evalue=8e-41,
Organism=Drosophila melanogaster, GI45550900, Length=433, Percent_Identity=28.175519630485, Blast_Score=158, Evalue=8e-39,
Organism=Drosophila melanogaster, GI45553807, Length=447, Percent_Identity=27.9642058165548, Blast_Score=155, Evalue=4e-38,
Organism=Drosophila melanogaster, GI45553816, Length=447, Percent_Identity=27.9642058165548, Blast_Score=155, Evalue=4e-38,
Organism=Drosophila melanogaster, GI24651721, Length=447, Percent_Identity=27.9642058165548, Blast_Score=155, Evalue=4e-38,
Organism=Drosophila melanogaster, GI17864154, Length=447, Percent_Identity=27.9642058165548, Blast_Score=155, Evalue=4e-38,
Organism=Drosophila melanogaster, GI17137380, Length=350, Percent_Identity=28.5714285714286, Blast_Score=142, Evalue=5e-34,
Organism=Drosophila melanogaster, GI19921738, Length=233, Percent_Identity=29.6137339055794, Blast_Score=89, Evalue=8e-18,
Organism=Drosophila melanogaster, GI281363316, Length=144, Percent_Identity=36.1111111111111, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI17864358, Length=144, Percent_Identity=36.1111111111111, Blast_Score=86, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR011779
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: =2.7.7.4 [H]

Molecular weight: Translated: 46489; Mature: 46357

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAPEQLPEIDNGLLRFLTCGSVDDGKSTLIGRLLFDTKAILADTMNAIEKTSAKRGMSA
CCCCHHCCHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
VDLSLLTDGLQAEREQGITIDVAYRYFSTGTRKYIIADAPGHEQYTRNMVTAASTANLAI
HHHHHHHHHHHHHHHCCCEEEEEEEECCCCCEEEEEECCCCCHHHHHHHHHEECCCCEEE
ILVDARRGVLTQTRRHSYLASLVGIPHLLVAVNKMDLVDYDQKVFEQIRADYLAFADKLG
EEEECCCCHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCC
IKDVRFIPISALAGDMIVDRGDRLGWYDGPTLLEILEAAPAAHTETAENFRFPVQFVCRP
CCCEEEEEHHHHCCCHHEECCCCCCCCCCHHHHHHHHHCCCCCCCCHHCCCCCEEEEECC
QDSANPELHDYRGFMGRVESGEVAVGDAITVLPSGATSKVKAIELGGEKLQRAIHEQSVT
CCCCCCCHHHHHHHHCCCCCCCEEECCEEEEECCCCCCCEEEEECCHHHHHHHHHHCCEE
LLLEDEIDISRGDMIVKSAEAPEAVKQIDATVCWLSETPMSTARTYLVRHTTREAKAKVA
EEEECCCCCCCCCEEEECCCCHHHHHHHHHEEEEECCCCHHHHHHHHHHHHHHHHHHHEE
KIDYRLDVNTLEQEPATSLAMNDIARLTLKLAQPIVADAYADNRATGAFIIIDESTNNTV
EEEEEECCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCC
GAGMIG
CCCCCC
>Mature Secondary Structure 
SAPEQLPEIDNGLLRFLTCGSVDDGKSTLIGRLLFDTKAILADTMNAIEKTSAKRGMSA
CCCHHCCHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
VDLSLLTDGLQAEREQGITIDVAYRYFSTGTRKYIIADAPGHEQYTRNMVTAASTANLAI
HHHHHHHHHHHHHHHCCCEEEEEEEECCCCCEEEEEECCCCCHHHHHHHHHEECCCCEEE
ILVDARRGVLTQTRRHSYLASLVGIPHLLVAVNKMDLVDYDQKVFEQIRADYLAFADKLG
EEEECCCCHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCC
IKDVRFIPISALAGDMIVDRGDRLGWYDGPTLLEILEAAPAAHTETAENFRFPVQFVCRP
CCCEEEEEHHHHCCCHHEECCCCCCCCCCHHHHHHHHHCCCCCCCCHHCCCCCEEEEECC
QDSANPELHDYRGFMGRVESGEVAVGDAITVLPSGATSKVKAIELGGEKLQRAIHEQSVT
CCCCCCCHHHHHHHHCCCCCCCEEECCEEEEECCCCCCCEEEEECCHHHHHHHHHHCCEE
LLLEDEIDISRGDMIVKSAEAPEAVKQIDATVCWLSETPMSTARTYLVRHTTREAKAKVA
EEEECCCCCCCCCEEEECCCCHHHHHHHHHEEEEECCCCHHHHHHHHHHHHHHHHHHHEE
KIDYRLDVNTLEQEPATSLAMNDIARLTLKLAQPIVADAYADNRATGAFIIIDESTNNTV
EEEEEECCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCC
GAGMIG
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA