Click here to switch to the map view.

The map label for this gene is fadAx [H]

Identifier: 119896684

GI number: 119896684

Start: 419104

End: 420315

Strand: Direct

Name: fadAx [H]

Synonym: azo0393

Alternate gene names: 119896684

Gene position: 419104-420315 (Clockwise)

Preceding gene: 119896683

Following gene: 119896685

Centisome position: 9.58

GC content: 69.06

Gene sequence:

>1212_bases
ATGTCCGATCCCGTAGTCATCGTTTCCGTCGCCCGCACGCCGATGGGCGGCTTCCAGGGCGATCTTTCCGGCCTCACCGG
GCCGCAGCTCGGCGCCATCGCGATCAAGTCGGCGGTGGAGCGCGCGCGTCTTGCGCCCGAGCAGGTGCAGGAGGTGATCA
TGGGTTGCGTGCTGCCGGCCGGCGTCGGCCAGGCGCCGGCGCGTCAGGCGGCACTCGGTGCCGGGCTGCCGCTGGCGGCG
GGCTGCACGACCATCAACAAGGTCTGCGGGTCCGGCATGAAGGCGACGATGCTGGCCCACGACCTCCTGTTGGCGGGCAC
CAACGAGGTGATGGTGGCGGGCGGCATGGAGTCGATGTCGAACGCGCCGTATCTGTTGCCGAAGGCGCGCGGCGGTTATC
GCCTGGGCCACGGCCAGTTGCTCGACCACATGTTCCTCGACGGGCTGGAAGACAGCTATTCGAAGGAGAACAAGGGCCGG
CTGATGGGCACCTTTGCCGAGGATTGCGCCGGCCATTTCGATTTCAGCCGCAGCGCGCAGGACGCGTTCGCGGTGGCCTC
CACCGAGCGCGCGCAGGCGGCGATCACCGAAGGCGCCTTCAGCTGGGAAGTGGTGCCGGTGACGGTGGCCGGGCGCAAGG
GCGATGTGGTGGTGGACAAGGACGAGCAGCCGCTGAAGGCGCAGCTCGACAAGATCGGCACGCTGAAGCCGGCGTTCAAG
AAGGACGGCACGGTGACCGCGGCGAATTCGTCGTCGATTTCGGATGGTGCGGCGGCGCTGGTGCTGATGCGCAAGTCGAC
GGCCGACAAGCTGGGGCTGGCGCCGCTGGCGACCATCGTCGGCCACGCCACCCACGCGCAGGAGCCGCAGTGGTTCACCA
CCGCGCCGGTGGGGGCGATGCAGAAGGTGCTGGCGAAGGCGGGCTGGGGCGTGGGCGACGTCGATCTGTGGGAGATCAAC
GAGGCCTTCGCGGTGGTGACGATGGCGGCGATGAAGGAGCTGAACCTGCCGCACGACAAGGTCAATGTGAATGGCGGCGC
ATGTGCGCTGGGCCATCCGATCGGCGCGTCCGGTGCCCGCATCCTGGTGACGCTGATCGGTGCGCTGCGCCGGCGCGGGC
TGAAGCGCGGCGTGGCCAGCTTGTGCATCGGCGGCGGCGAGGCCACGGCGATGGCGATCGAACTCAACGCATCGGTGGCC
TGCGGCGGTTGA

Upstream 100 bases:

>100_bases
GGCAAGAAGGAACTCGCCGGCTGAGTATTCGCCGGCACCCCCGCCGCGCCCGTTCCGGGGGCGCGGCGCAAGAACCCACA
GACAGCAACAGGAGAGCAGC

Downstream 100 bases:

>100_bases
TCGTTCGTATGTTCCCGGCGGTTTGAGTGTTGAACCGCCGGCCGCGCTGGGTGCTTGTTCCGCGTGCTGCGGAACTCGCC
CTCCGGGCTCGGACAGTCCT

Product: acyl-CoA thiolase

Products: NA

Alternate protein names: Acetoacetyl-CoA thiolase [H]

Number of amino acids: Translated: 403; Mature: 402

Protein sequence:

>403_residues
MSDPVVIVSVARTPMGGFQGDLSGLTGPQLGAIAIKSAVERARLAPEQVQEVIMGCVLPAGVGQAPARQAALGAGLPLAA
GCTTINKVCGSGMKATMLAHDLLLAGTNEVMVAGGMESMSNAPYLLPKARGGYRLGHGQLLDHMFLDGLEDSYSKENKGR
LMGTFAEDCAGHFDFSRSAQDAFAVASTERAQAAITEGAFSWEVVPVTVAGRKGDVVVDKDEQPLKAQLDKIGTLKPAFK
KDGTVTAANSSSISDGAAALVLMRKSTADKLGLAPLATIVGHATHAQEPQWFTTAPVGAMQKVLAKAGWGVGDVDLWEIN
EAFAVVTMAAMKELNLPHDKVNVNGGACALGHPIGASGARILVTLIGALRRRGLKRGVASLCIGGGEATAMAIELNASVA
CGG

Sequences:

>Translated_403_residues
MSDPVVIVSVARTPMGGFQGDLSGLTGPQLGAIAIKSAVERARLAPEQVQEVIMGCVLPAGVGQAPARQAALGAGLPLAA
GCTTINKVCGSGMKATMLAHDLLLAGTNEVMVAGGMESMSNAPYLLPKARGGYRLGHGQLLDHMFLDGLEDSYSKENKGR
LMGTFAEDCAGHFDFSRSAQDAFAVASTERAQAAITEGAFSWEVVPVTVAGRKGDVVVDKDEQPLKAQLDKIGTLKPAFK
KDGTVTAANSSSISDGAAALVLMRKSTADKLGLAPLATIVGHATHAQEPQWFTTAPVGAMQKVLAKAGWGVGDVDLWEIN
EAFAVVTMAAMKELNLPHDKVNVNGGACALGHPIGASGARILVTLIGALRRRGLKRGVASLCIGGGEATAMAIELNASVA
CGG
>Mature_402_residues
SDPVVIVSVARTPMGGFQGDLSGLTGPQLGAIAIKSAVERARLAPEQVQEVIMGCVLPAGVGQAPARQAALGAGLPLAAG
CTTINKVCGSGMKATMLAHDLLLAGTNEVMVAGGMESMSNAPYLLPKARGGYRLGHGQLLDHMFLDGLEDSYSKENKGRL
MGTFAEDCAGHFDFSRSAQDAFAVASTERAQAAITEGAFSWEVVPVTVAGRKGDVVVDKDEQPLKAQLDKIGTLKPAFKK
DGTVTAANSSSISDGAAALVLMRKSTADKLGLAPLATIVGHATHAQEPQWFTTAPVGAMQKVLAKAGWGVGDVDLWEINE
AFAVVTMAAMKELNLPHDKVNVNGGACALGHPIGASGARILVTLIGALRRRGLKRGVASLCIGGGEATAMAIELNASVAC
GG

Specific function: SHORT-CHAIN FATTY ACIDS METABOLISM. [C]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI4557237, Length=392, Percent_Identity=50.2551020408163, Blast_Score=347, Evalue=1e-95,
Organism=Homo sapiens, GI148539872, Length=396, Percent_Identity=48.7373737373737, Blast_Score=339, Evalue=3e-93,
Organism=Homo sapiens, GI167614485, Length=395, Percent_Identity=38.7341772151899, Blast_Score=250, Evalue=2e-66,
Organism=Homo sapiens, GI4501853, Length=398, Percent_Identity=36.1809045226131, Blast_Score=214, Evalue=1e-55,
Organism=Homo sapiens, GI4504327, Length=421, Percent_Identity=32.541567695962, Blast_Score=168, Evalue=8e-42,
Organism=Homo sapiens, GI194353979, Length=89, Percent_Identity=51.685393258427, Blast_Score=89, Evalue=8e-18,
Organism=Escherichia coli, GI1788554, Length=391, Percent_Identity=50.8951406649616, Blast_Score=334, Evalue=6e-93,
Organism=Escherichia coli, GI87082165, Length=395, Percent_Identity=44.8101265822785, Blast_Score=320, Evalue=9e-89,
Organism=Escherichia coli, GI1787663, Length=393, Percent_Identity=37.6590330788804, Blast_Score=236, Evalue=1e-63,
Organism=Escherichia coli, GI48994986, Length=404, Percent_Identity=36.1386138613861, Blast_Score=194, Evalue=8e-51,
Organism=Escherichia coli, GI1788683, Length=402, Percent_Identity=30.0995024875622, Blast_Score=150, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI25147385, Length=388, Percent_Identity=42.7835051546392, Blast_Score=308, Evalue=2e-84,
Organism=Caenorhabditis elegans, GI17535921, Length=397, Percent_Identity=44.3324937027708, Blast_Score=300, Evalue=8e-82,
Organism=Caenorhabditis elegans, GI133906874, Length=391, Percent_Identity=38.3631713554987, Blast_Score=270, Evalue=1e-72,
Organism=Caenorhabditis elegans, GI17535917, Length=398, Percent_Identity=35.427135678392, Blast_Score=203, Evalue=1e-52,
Organism=Caenorhabditis elegans, GI17551802, Length=423, Percent_Identity=30.9692671394799, Blast_Score=168, Evalue=5e-42,
Organism=Saccharomyces cerevisiae, GI6325229, Length=400, Percent_Identity=47, Blast_Score=329, Evalue=4e-91,
Organism=Saccharomyces cerevisiae, GI6322031, Length=393, Percent_Identity=32.824427480916, Blast_Score=181, Evalue=2e-46,
Organism=Drosophila melanogaster, GI24655093, Length=396, Percent_Identity=46.969696969697, Blast_Score=335, Evalue=3e-92,
Organism=Drosophila melanogaster, GI24640423, Length=392, Percent_Identity=47.9591836734694, Blast_Score=333, Evalue=2e-91,
Organism=Drosophila melanogaster, GI17648125, Length=394, Percent_Identity=37.5634517766497, Blast_Score=236, Evalue=2e-62,
Organism=Drosophila melanogaster, GI17137578, Length=424, Percent_Identity=32.7830188679245, Blast_Score=172, Evalue=4e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.9 [H]

Molecular weight: Translated: 41363; Mature: 41232

Theoretical pI: Translated: 7.19; Mature: 7.19

Prosite motif: PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDPVVIVSVARTPMGGFQGDLSGLTGPQLGAIAIKSAVERARLAPEQVQEVIMGCVLPA
CCCCEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCC
GVGQAPARQAALGAGLPLAAGCTTINKVCGSGMKATMLAHDLLLAGTNEVMVAGGMESMS
CCCCCCHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEECCHHHHC
NAPYLLPKARGGYRLGHGQLLDHMFLDGLEDSYSKENKGRLMGTFAEDCAGHFDFSRSAQ
CCCEEEECCCCCEECCHHHHHHHHHHHCCCHHHCCCCCCCEEEEHHHHHCCCCCCCCCCH
DAFAVASTERAQAAITEGAFSWEVVPVTVAGRKGDVVVDKDEQPLKAQLDKIGTLKPAFK
HHHHHHCCHHHHHHHHCCCCEEEEEEEEEECCCCCEEECCCCCCHHHHHHHHCCCCCCCC
KDGTVTAANSSSISDGAAALVLMRKSTADKLGLAPLATIVGHATHAQEPQWFTTAPVGAM
CCCEEEECCCCCCCCCCEEEEEEECCCCHHHCHHHHHHHHHCCCCCCCCCEEECCCHHHH
QKVLAKAGWGVGDVDLWEINEAFAVVTMAAMKELNLPHDKVNVNGGACALGHPIGASGAR
HHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCEEECCCCEEEECCCCCCCHHH
ILVTLIGALRRRGLKRGVASLCIGGGEATAMAIELNASVACGG
HHHHHHHHHHHHHHHHHHHHHEECCCCEEEEEEEECCEEECCC
>Mature Secondary Structure 
SDPVVIVSVARTPMGGFQGDLSGLTGPQLGAIAIKSAVERARLAPEQVQEVIMGCVLPA
CCCEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCC
GVGQAPARQAALGAGLPLAAGCTTINKVCGSGMKATMLAHDLLLAGTNEVMVAGGMESMS
CCCCCCHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEECCHHHHC
NAPYLLPKARGGYRLGHGQLLDHMFLDGLEDSYSKENKGRLMGTFAEDCAGHFDFSRSAQ
CCCEEEECCCCCEECCHHHHHHHHHHHCCCHHHCCCCCCCEEEEHHHHHCCCCCCCCCCH
DAFAVASTERAQAAITEGAFSWEVVPVTVAGRKGDVVVDKDEQPLKAQLDKIGTLKPAFK
HHHHHHCCHHHHHHHHCCCCEEEEEEEEEECCCCCEEECCCCCCHHHHHHHHCCCCCCCC
KDGTVTAANSSSISDGAAALVLMRKSTADKLGLAPLATIVGHATHAQEPQWFTTAPVGAM
CCCEEEECCCCCCCCCCEEEEEEECCCCHHHCHHHHHHHHHCCCCCCCCCEEECCCHHHH
QKVLAKAGWGVGDVDLWEINEAFAVVTMAAMKELNLPHDKVNVNGGACALGHPIGASGAR
HHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCEEECCCCEEEECCCCCCCHHH
ILVTLIGALRRRGLKRGVASLCIGGGEATAMAIELNASVACGG
HHHHHHHHHHHHHHHHHHHHHEECCCCEEEEEEEECCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7867955; 11075929; 11466286; 1685080 [H]