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The map label for this gene is paaE [H]

Identifier: 119896599

GI number: 119896599

Start: 331742

End: 332809

Strand: Direct

Name: paaE [H]

Synonym: azo0308

Alternate gene names: 119896599

Gene position: 331742-332809 (Clockwise)

Preceding gene: 119896598

Following gene: 119896600

Centisome position: 7.58

GC content: 68.91

Gene sequence:

>1068_bases
ATGACGCCACGATTCCACCCGCTCAAGGTCGCCGAAGTGCGCCGCGAAACCGCCGACTCGGTGAGCCTGCGCTTCGAGGT
GCCGGCCGACCTCGCCGCCGACTACCGCTTCGTGCAGGGCCAGCATCTCAACCTCAAGGCCGTGGTGAACGGCGAGGAAG
TGCGCCGCTCGTACTCCATCTGCTCCGGCGTCGATGACGGCGAGCTGCGGGTGGCGATCCGCAAGGTCGATGGCGGCCGC
TTCTCCAGCTGGGCGGTGGACGCGGTGCGCGTGGGCGACGTGTTCGAGGTGATGACGCCCGAAGGCCGCTTCTCCACCCA
GCTCGACCCCGCCAACGCGCACCACTACGTCGCCTTCGCCGCGGGCAGCGGCATCACCCCCATCCTGTCGCTGATCAAGA
CCACGCTGCGCGCCGAGCCGAAGAGCCGCTTCACGCTGGTGTACGGCAACCGCAACCAGAACAGCGCGATGTTCGCCGAG
GCGCTGGAAGACCTGAAGGACCGCTACCTGACCCGCTTCGCGCTCTACAACGTGTTCTCGCGCGAGGAGCAGGAGGTGCC
GCTGTTCAACGGCCGCCTCGACCAGGCCCGCGTCGCCGCCTTCCTCGACACCCTGATCCCCGCCGCCGACATCGACGCCG
CCTTCATCTGCGGCCCCGGCGGCATGATCGACGAGGTCGAGGCCGCGCTGAAGGGCGCCGGCGTGCCCGCCGACCGCATC
CACCTCGAACGCTTCGGCGTGCCCGCGAGCGCACCGCGCCACCACGTGGAGGCGGGCGACGCACCGCAGGCGCGCATCAC
CGTGATCGTGGACGGCCTCAAGCGCGAGATGGAATTCCGCGCGCAGGACCCCTCCATCCTCGACGTCGCGCTGCGCGCCG
GGCTGGACCTGCCGTACTCGTGCAAGGGTGGGGTATGCTGCACCTGCCGCGCCAAGGTGCTCGAAGGCAAGGTCCGCATG
GACAAGAACTACACGCTGGAGCAGCCGGACATCGACGCGGGCTATGTGCTTACCTGCCAGGCCCACCCGCTGACCGAGCG
CGTGGTGATCAGCTACGACGACAGATAA

Upstream 100 bases:

>100_bases
CCGCGTGCAAGGCCACCTGGCGCTGCCGCGCCTGCCTCGAACCGTTCGAATACTTCAAACCGATCTGATCCGGGCGATCG
ACCCGGACACGGAGACAGAC

Downstream 100 bases:

>100_bases
GGCGGCCGAACCGGTTTCCGGCGCGAAGCCGCTGTTCGCCTGCGGAATACGGACGACACATGGCCAGGGGCAAATCTCCC
ACCTTCGAACTGCAACGCGA

Product: phenylacetic acid degradation NADH oxidoreductase PaaE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 355; Mature: 354

Protein sequence:

>355_residues
MTPRFHPLKVAEVRRETADSVSLRFEVPADLAADYRFVQGQHLNLKAVVNGEEVRRSYSICSGVDDGELRVAIRKVDGGR
FSSWAVDAVRVGDVFEVMTPEGRFSTQLDPANAHHYVAFAAGSGITPILSLIKTTLRAEPKSRFTLVYGNRNQNSAMFAE
ALEDLKDRYLTRFALYNVFSREEQEVPLFNGRLDQARVAAFLDTLIPAADIDAAFICGPGGMIDEVEAALKGAGVPADRI
HLERFGVPASAPRHHVEAGDAPQARITVIVDGLKREMEFRAQDPSILDVALRAGLDLPYSCKGGVCCTCRAKVLEGKVRM
DKNYTLEQPDIDAGYVLTCQAHPLTERVVISYDDR

Sequences:

>Translated_355_residues
MTPRFHPLKVAEVRRETADSVSLRFEVPADLAADYRFVQGQHLNLKAVVNGEEVRRSYSICSGVDDGELRVAIRKVDGGR
FSSWAVDAVRVGDVFEVMTPEGRFSTQLDPANAHHYVAFAAGSGITPILSLIKTTLRAEPKSRFTLVYGNRNQNSAMFAE
ALEDLKDRYLTRFALYNVFSREEQEVPLFNGRLDQARVAAFLDTLIPAADIDAAFICGPGGMIDEVEAALKGAGVPADRI
HLERFGVPASAPRHHVEAGDAPQARITVIVDGLKREMEFRAQDPSILDVALRAGLDLPYSCKGGVCCTCRAKVLEGKVRM
DKNYTLEQPDIDAGYVLTCQAHPLTERVVISYDDR
>Mature_354_residues
TPRFHPLKVAEVRRETADSVSLRFEVPADLAADYRFVQGQHLNLKAVVNGEEVRRSYSICSGVDDGELRVAIRKVDGGRF
SSWAVDAVRVGDVFEVMTPEGRFSTQLDPANAHHYVAFAAGSGITPILSLIKTTLRAEPKSRFTLVYGNRNQNSAMFAEA
LEDLKDRYLTRFALYNVFSREEQEVPLFNGRLDQARVAAFLDTLIPAADIDAAFICGPGGMIDEVEAALKGAGVPADRIH
LERFGVPASAPRHHVEAGDAPQARITVIVDGLKREMEFRAQDPSILDVALRAGLDLPYSCKGGVCCTCRAKVLEGKVRMD
KNYTLEQPDIDAGYVLTCQAHPLTERVVISYDDR

Specific function: May be part of a multicomponent oxygenase involved in phenylacetyl-CoA hydroxylation [H]

COG id: COG1018

COG function: function code C; Flavodoxin reductases (ferredoxin-NADPH reductases) family 1

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Homo sapiens, GI49574502, Length=209, Percent_Identity=26.7942583732057, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1787658, Length=351, Percent_Identity=49.8575498575499, Blast_Score=348, Evalue=3e-97,
Organism=Escherichia coli, GI1787098, Length=340, Percent_Identity=26.4705882352941, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1788903, Length=234, Percent_Identity=26.4957264957265, Blast_Score=74, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17563928, Length=246, Percent_Identity=25.2032520325203, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17563926, Length=211, Percent_Identity=25.1184834123223, Blast_Score=72, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006058
- InterPro:   IPR012675
- InterPro:   IPR017927
- InterPro:   IPR001041
- InterPro:   IPR001709
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR011884
- InterPro:   IPR001221
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00111 Fer2; PF00175 NAD_binding_1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 39111; Mature: 38980

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: PS51085 2FE2S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPRFHPLKVAEVRRETADSVSLRFEVPADLAADYRFVQGQHLNLKAVVNGEEVRRSYSI
CCCCCCCCHHHHHHHHCCCCEEEEEECCCCHHHCEEEECCCCEEEEEEECHHHHHHHHHH
CSGVDDGELRVAIRKVDGGRFSSWAVDAVRVGDVFEVMTPEGRFSTQLDPANAHHYVAFA
HCCCCCCCEEEEEEECCCCCCCCEEEEEEEECCEEEEECCCCCCCCCCCCCCCCEEEEEE
AGSGITPILSLIKTTLRAEPKSRFTLVYGNRNQNSAMFAEALEDLKDRYLTRFALYNVFS
CCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
REEQEVPLFNGRLDQARVAAFLDTLIPAADIDAAFICGPGGMIDEVEAALKGAGVPADRI
CCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCHHHE
HLERFGVPASAPRHHVEAGDAPQARITVIVDGLKREMEFRAQDPSILDVALRAGLDLPYS
EHHHCCCCCCCCCCCCCCCCCCCEEEEEEEECHHHHHHHCCCCCHHHHHHHHHCCCCCCC
CKGGVCCTCRAKVLEGKVRMDKNYTLEQPDIDAGYVLTCQAHPLTERVVISYDDR
CCCCEEEEEEHHHHCCEEEECCCCCCCCCCCCCCEEEEEECCCCCCEEEEEECCC
>Mature Secondary Structure 
TPRFHPLKVAEVRRETADSVSLRFEVPADLAADYRFVQGQHLNLKAVVNGEEVRRSYSI
CCCCCCCHHHHHHHHCCCCEEEEEECCCCHHHCEEEECCCCEEEEEEECHHHHHHHHHH
CSGVDDGELRVAIRKVDGGRFSSWAVDAVRVGDVFEVMTPEGRFSTQLDPANAHHYVAFA
HCCCCCCCEEEEEEECCCCCCCCEEEEEEEECCEEEEECCCCCCCCCCCCCCCCEEEEEE
AGSGITPILSLIKTTLRAEPKSRFTLVYGNRNQNSAMFAEALEDLKDRYLTRFALYNVFS
CCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
REEQEVPLFNGRLDQARVAAFLDTLIPAADIDAAFICGPGGMIDEVEAALKGAGVPADRI
CCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCHHHE
HLERFGVPASAPRHHVEAGDAPQARITVIVDGLKREMEFRAQDPSILDVALRAGLDLPYS
EHHHCCCCCCCCCCCCCCCCCCCEEEEEEEECHHHHHHHCCCCCHHHHHHHHHCCCCCCC
CKGGVCCTCRAKVLEGKVRMDKNYTLEQPDIDAGYVLTCQAHPLTERVVISYDDR
CCCCEEEEEEHHHHCCEEEECCCCCCCCCCCCCCEEEEEECCCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD [C]

Metal ions: Iron-Sulfur [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9748275; 9097039; 9278503; 10766858 [H]