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The map label for this gene is gcvA [H]

Identifier: 119896424

GI number: 119896424

Start: 147273

End: 148154

Strand: Direct

Name: gcvA [H]

Synonym: azo0132

Alternate gene names: 119896424

Gene position: 147273-148154 (Clockwise)

Preceding gene: 119896423

Following gene: 119896425

Centisome position: 3.37

GC content: 73.81

Gene sequence:

>882_bases
ATGCGTCTGCCCCCGCTGCATGCCCTCGTCGCCTTCGAAGCCGCCGCCCGCGCGGGCAGTTTTCTTGCAGCGGCCGCGGA
ATTGCACCTGACGCCGTCGGCCGTCAGCCACCGCATCAAGGCGCTGGAAGACTGGCTGGGCACGGCGCTGTTCGAACGCC
GCCAGCGCCTCGTGGTGCTGACCGAGGCGGGGCGCGATTACCTCGCACAGATCCAGCCCGCGCTCGCCGACATCGAAACC
GCCAGCGCCGCGATGCAGGCGCCGCACAGCCGCGTGCTGCGGCTGTCGGTGGCGCCGGCGATCGGCGCGAAATGGTTCGT
CGGCCAGCTGGCTGCCTACCGCGAGGTCGATCCCGGGCTCGAATTCGTGCTCTCCACCGCGTCGACGATGGCGCCGGTCG
CGGCCGGCAAGGCCGACGTGGGCCTGTGCTACGGCGAACCGCCGTGGCCCGGGCTGGAAGCCTACGAACTGCGCCGTGAG
ACCGTGTTTCCGGTGTGCAACCCGGCGCTCGCGGCGCGTCTGGGCGATCCGCCCGCAGCCGCTGCGCTGGCGGCGCTGCC
CTTGTTGCGCCACCCGCTGCTCGCGTGGCGGCCGTGGTTCGCCGCGGTCGGGCTGGATCGCGCCGAGCCGGCCGAGGGGC
CGCTGTTCGAGGACGCGATGATGATGCTGGAAGCGGCCAGCGCCGGCGTGGGCGTGGCCTTGACCGTGGAACTGCTGGCG
CGCCCCTATCTCGCCAGCGGCGCGCTGCAGCGCCCGTTCGCCGTCGCCGTGCCGGGCAAGGCCTTCCATGCCGTGCTGCG
TGCCGACGCCTACGGCCGGCCCTGGGTGCGCCGCTTCGTGCGCTGGCTGCAGGGGCGCGCGCGCCGCGACACCGCCGCCT
GA

Upstream 100 bases:

>100_bases
GGCGTCATGCGGCGAACGGCATTGCCCTTTCGACGGCGCGGTCAATAGTATGCGGGGCCGGGCGGACCGCGCCCGCACTG
AACCCGTCCGGATTTCCCCG

Downstream 100 bases:

>100_bases
GGGCCGGCGCCCGGTTGAATCCATTTCATCTGAAAGCCCTTGCTTTCGTTTGTCCGCCTGGCGCGCGGACGGCACGCTGT
GTCCCACCCGCTGTTGCCGT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MRLPPLHALVAFEAAARAGSFLAAAAELHLTPSAVSHRIKALEDWLGTALFERRQRLVVLTEAGRDYLAQIQPALADIET
ASAAMQAPHSRVLRLSVAPAIGAKWFVGQLAAYREVDPGLEFVLSTASTMAPVAAGKADVGLCYGEPPWPGLEAYELRRE
TVFPVCNPALAARLGDPPAAAALAALPLLRHPLLAWRPWFAAVGLDRAEPAEGPLFEDAMMMLEAASAGVGVALTVELLA
RPYLASGALQRPFAVAVPGKAFHAVLRADAYGRPWVRRFVRWLQGRARRDTAA

Sequences:

>Translated_293_residues
MRLPPLHALVAFEAAARAGSFLAAAAELHLTPSAVSHRIKALEDWLGTALFERRQRLVVLTEAGRDYLAQIQPALADIET
ASAAMQAPHSRVLRLSVAPAIGAKWFVGQLAAYREVDPGLEFVLSTASTMAPVAAGKADVGLCYGEPPWPGLEAYELRRE
TVFPVCNPALAARLGDPPAAAALAALPLLRHPLLAWRPWFAAVGLDRAEPAEGPLFEDAMMMLEAASAGVGVALTVELLA
RPYLASGALQRPFAVAVPGKAFHAVLRADAYGRPWVRRFVRWLQGRARRDTAA
>Mature_293_residues
MRLPPLHALVAFEAAARAGSFLAAAAELHLTPSAVSHRIKALEDWLGTALFERRQRLVVLTEAGRDYLAQIQPALADIET
ASAAMQAPHSRVLRLSVAPAIGAKWFVGQLAAYREVDPGLEFVLSTASTMAPVAAGKADVGLCYGEPPWPGLEAYELRRE
TVFPVCNPALAARLGDPPAAAALAALPLLRHPLLAWRPWFAAVGLDRAEPAEGPLFEDAMMMLEAASAGVGVALTVELLA
RPYLASGALQRPFAVAVPGKAFHAVLRADAYGRPWVRRFVRWLQGRARRDTAA

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=294, Percent_Identity=34.6938775510204, Blast_Score=163, Evalue=1e-41,
Organism=Escherichia coli, GI1786448, Length=255, Percent_Identity=35.6862745098039, Blast_Score=141, Evalue=5e-35,
Organism=Escherichia coli, GI1788706, Length=297, Percent_Identity=29.2929292929293, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI145693193, Length=263, Percent_Identity=25.4752851711027, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787128, Length=298, Percent_Identity=26.510067114094, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1787949, Length=80, Percent_Identity=43.75, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI87081978, Length=205, Percent_Identity=28.780487804878, Blast_Score=64, Evalue=8e-12,
Organism=Escherichia coli, GI1790262, Length=233, Percent_Identity=26.1802575107296, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1787806, Length=238, Percent_Identity=27.3109243697479, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 31387; Mature: 31387

Theoretical pI: Translated: 9.11; Mature: 9.11

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLPPLHALVAFEAAARAGSFLAAAAELHLTPSAVSHRIKALEDWLGTALFERRQRLVVL
CCCCHHHHHHHHHHHHHHCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
TEAGRDYLAQIQPALADIETASAAMQAPHSRVLRLSVAPAIGAKWFVGQLAAYREVDPGL
ECCCHHHHHHHHHHHHHHHHHHHHHHCCHHHEEEEEECCHHCHHHHHHHHHHHHHCCCHH
EFVLSTASTMAPVAAGKADVGLCYGEPPWPGLEAYELRRETVFPVCNPALAARLGDPPAA
HHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHCCCCCCHHHHHHCCCCHHH
AALAALPLLRHPLLAWRPWFAAVGLDRAEPAEGPLFEDAMMMLEAASAGVGVALTVELLA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHH
RPYLASGALQRPFAVAVPGKAFHAVLRADAYGRPWVRRFVRWLQGRARRDTAA
CHHHHCCCCCCCEEEECCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MRLPPLHALVAFEAAARAGSFLAAAAELHLTPSAVSHRIKALEDWLGTALFERRQRLVVL
CCCCHHHHHHHHHHHHHHCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
TEAGRDYLAQIQPALADIETASAAMQAPHSRVLRLSVAPAIGAKWFVGQLAAYREVDPGL
ECCCHHHHHHHHHHHHHHHHHHHHHHCCHHHEEEEEECCHHCHHHHHHHHHHHHHCCCHH
EFVLSTASTMAPVAAGKADVGLCYGEPPWPGLEAYELRRETVFPVCNPALAARLGDPPAA
HHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHCCCCCCHHHHHHCCCCHHH
AALAALPLLRHPLLAWRPWFAAVGLDRAEPAEGPLFEDAMMMLEAASAGVGVALTVELLA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHH
RPYLASGALQRPFAVAVPGKAFHAVLRADAYGRPWVRRFVRWLQGRARRDTAA
CHHHHCCCCCCCEEEECCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]