Click here to switch to the map view.

The map label for this gene is def [H]

Identifier: 119896391

GI number: 119896391

Start: 118530

End: 119033

Strand: Direct

Name: def [H]

Synonym: azo0099

Alternate gene names: 119896391

Gene position: 118530-119033 (Clockwise)

Preceding gene: 119896386

Following gene: 119896392

Centisome position: 2.71

GC content: 65.48

Gene sequence:

>504_bases
ATGGCTCTTCTCCCCATCCTGCGCTACCCCGATCCACGGCTTCATCGGCGCGCCGCGCCCGTCGACACGGTCGACGATGA
CGTACGCAAGCTGATCGACGACATGGCCGAGACCATGTACGAGGCGCCCGGCATCGGCCTGGCCGCGACCCAGGTGGACG
TGCACCGGCGCGTCGTCGTGATTGACATCTCCGAGGACAAGTCCGGCCTGATGGCGCTGATCAATCCCCAGATCCTCGAA
CGCGACGGCGAGCAGGTGTGCGAGGAAGGCTGCCTGTCGGTGCCCGGTGTTTACGAAAAGGTCACCCGTGCCGAACGCGT
CACGGTGCGTGCGCTCGACCGCAACGGCCACCCGTTCGAAGTGGCTGCCGAAGGCCTGCTCGCGGTGTGCATCCAGCACG
AAATCGACCACCTCGACGGCAAGGTCTTCGTCGAGTACCTGTCGCCGCTCAAGCTCGGCCGCATCAAGAGCAAGCTGGCC
AAGAAGGCGCGCATCACCGCCTGA

Upstream 100 bases:

>100_bases
CCACCCCAGCGCCGTCGGAACCCTGCCCGGCACAGCACGTGGGCATGCATGTCAAATCGCTTGAAATTCTGCACTTAAAG
GCTTATCCCGGCAAGCATTT

Downstream 100 bases:

>100_bases
TCCACGGCGGCGCCTGTCGCCGCCCCGCTTTTCCCGGTTCCGCCCATGTCCCTTCCGCTCCGTGTCGCCTTCGCCGGCAC
GCCAGAATTCGCCGCCAGCG

Product: peptide deformylase

Products: NA

Alternate protein names: PDF 1; Polypeptide deformylase 1 [H]

Number of amino acids: Translated: 167; Mature: 166

Protein sequence:

>167_residues
MALLPILRYPDPRLHRRAAPVDTVDDDVRKLIDDMAETMYEAPGIGLAATQVDVHRRVVVIDISEDKSGLMALINPQILE
RDGEQVCEEGCLSVPGVYEKVTRAERVTVRALDRNGHPFEVAAEGLLAVCIQHEIDHLDGKVFVEYLSPLKLGRIKSKLA
KKARITA

Sequences:

>Translated_167_residues
MALLPILRYPDPRLHRRAAPVDTVDDDVRKLIDDMAETMYEAPGIGLAATQVDVHRRVVVIDISEDKSGLMALINPQILE
RDGEQVCEEGCLSVPGVYEKVTRAERVTVRALDRNGHPFEVAAEGLLAVCIQHEIDHLDGKVFVEYLSPLKLGRIKSKLA
KKARITA
>Mature_166_residues
ALLPILRYPDPRLHRRAAPVDTVDDDVRKLIDDMAETMYEAPGIGLAATQVDVHRRVVVIDISEDKSGLMALINPQILER
DGEQVCEEGCLSVPGVYEKVTRAERVTVRALDRNGHPFEVAAEGLLAVCIQHEIDHLDGKVFVEYLSPLKLGRIKSKLAK
KARITA

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Homo sapiens, GI11641243, Length=157, Percent_Identity=29.9363057324841, Blast_Score=65, Evalue=3e-11,
Organism=Escherichia coli, GI1789682, Length=167, Percent_Identity=55.0898203592814, Blast_Score=197, Evalue=3e-52,
Organism=Drosophila melanogaster, GI24645728, Length=158, Percent_Identity=32.9113924050633, Blast_Score=74, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 18546; Mature: 18415

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALLPILRYPDPRLHRRAAPVDTVDDDVRKLIDDMAETMYEAPGIGLAATQVDVHRRVVV
CCCCCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCEEEE
IDISEDKSGLMALINPQILERDGEQVCEEGCLSVPGVYEKVTRAERVTVRALDRNGHPFE
EEECCCCCCEEECCCHHHHHCCHHHHHHHCCCCCCHHHHHHHHHHHHHEEEECCCCCCHH
VAAEGLLAVCIQHEIDHLDGKVFVEYLSPLKLGRIKSKLAKKARITA
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
ALLPILRYPDPRLHRRAAPVDTVDDDVRKLIDDMAETMYEAPGIGLAATQVDVHRRVVV
CCCCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCEEEE
IDISEDKSGLMALINPQILERDGEQVCEEGCLSVPGVYEKVTRAERVTVRALDRNGHPFE
EEECCCCCCEEECCCHHHHHCCHHHHHHHCCCCCCHHHHHHHHHHHHHEEEECCCCCCHH
VAAEGLLAVCIQHEIDHLDGKVFVEYLSPLKLGRIKSKLAKKARITA
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12910271 [H]