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The map label for this gene is fmt [H]

Identifier: 119896392

GI number: 119896392

Start: 119079

End: 120044

Strand: Direct

Name: fmt [H]

Synonym: azo0100

Alternate gene names: 119896392

Gene position: 119079-120044 (Clockwise)

Preceding gene: 119896391

Following gene: 119896393

Centisome position: 2.72

GC content: 72.46

Gene sequence:

>966_bases
ATGTCCCTTCCGCTCCGTGTCGCCTTCGCCGGCACGCCAGAATTCGCCGCCAGCGCACTCGCCGCCATCCTCGACGCGGG
CTACTCCGTGCCCTTGGTGCTGACCCAGCCCGACCGCCCCGCCGGGCGCGGCATGAAGCTGACGCCCAGCCCGGTCAAGC
AGCTTGCGCTGGCGCGCGGGCTGGACGTCGACCAGCCGGAGAAGCTGCGTACCGATGAACAGCGTCAGCGCCTCGCCGCC
TGTGAGCCCGACGTGCTGGTGGTGGCGGCTTACGGCCTGATCCTGCCCGCGGCGGTGCTGCAGCTGCCGCGCTACGGTTG
CATCAACATCCACGCCTCGCTGTTGCCCCGCTGGCGCGGTGCCGCCCCCATCCACCGCGCCGTGGAGGCGGGCGACGCCG
AGACCGGAATCACCATCATGCAGATGGACGAAGGGCTCGATACCGGCGACATGCTGTTGCGGCGGGCGATCCCGATCCGC
CCCGACGACACCACTGGCACCCTGCACGACAAGCTCGCCGCGCTCGGTGCCGAATGCATCGTCGAGGCCCTCGCGGCGCT
GCCCGGCGGCACCCTGTCCCGCACGCCCCAGCCTGCGGCCGGGGTGACCTACGCGAACAAGATCCACCGCGACGAAGCGG
CCATCGACTGGACCCGCCCGGCGGCGGACATCGAGCGTGCGGTCCGCGCTTTCAACCCCTTCCCGGTGGCGGTGGGCAGC
CTGCGCGATACCGTGATCAAGGTGTGGGCGGCGGAGGTTGTCGCGGTCCCCGCGCCGGACGCGGCCCCGGGCACCGTGCT
GGCGGCGGATGCCGAGGGCGTGGTGGTCGCATGCGGGGCGCAGGCCTTGCGGCTGCGCGAACTGCAGCGCCCTGGCAGCC
GGCGCTTTGCGGCCGGGGAATTCCTGCGCGGCTTCCCGGTCTGTGCCGGGGAACGGTTCGCGGTTGGCGGAGCGCAAGCC
GGTTGA

Upstream 100 bases:

>100_bases
CAAGCTCGGCCGCATCAAGAGCAAGCTGGCCAAGAAGGCGCGCATCACCGCCTGATCCACGGCGGCGCCTGTCGCCGCCC
CGCTTTTCCCGGTTCCGCCC

Downstream 100 bases:

>100_bases
AAGCGGGGCCGTCGGCCCCATCTACCGCCGCAAGGAATCAGCTAGCAAGGATCACCCCCATGTTCGGTAACTGGATCAAG
ACTTCGATACTCATGGCCGG

Product: methionyl-tRNA formyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 321; Mature: 320

Protein sequence:

>321_residues
MSLPLRVAFAGTPEFAASALAAILDAGYSVPLVLTQPDRPAGRGMKLTPSPVKQLALARGLDVDQPEKLRTDEQRQRLAA
CEPDVLVVAAYGLILPAAVLQLPRYGCINIHASLLPRWRGAAPIHRAVEAGDAETGITIMQMDEGLDTGDMLLRRAIPIR
PDDTTGTLHDKLAALGAECIVEALAALPGGTLSRTPQPAAGVTYANKIHRDEAAIDWTRPAADIERAVRAFNPFPVAVGS
LRDTVIKVWAAEVVAVPAPDAAPGTVLAADAEGVVVACGAQALRLRELQRPGSRRFAAGEFLRGFPVCAGERFAVGGAQA
G

Sequences:

>Translated_321_residues
MSLPLRVAFAGTPEFAASALAAILDAGYSVPLVLTQPDRPAGRGMKLTPSPVKQLALARGLDVDQPEKLRTDEQRQRLAA
CEPDVLVVAAYGLILPAAVLQLPRYGCINIHASLLPRWRGAAPIHRAVEAGDAETGITIMQMDEGLDTGDMLLRRAIPIR
PDDTTGTLHDKLAALGAECIVEALAALPGGTLSRTPQPAAGVTYANKIHRDEAAIDWTRPAADIERAVRAFNPFPVAVGS
LRDTVIKVWAAEVVAVPAPDAAPGTVLAADAEGVVVACGAQALRLRELQRPGSRRFAAGEFLRGFPVCAGERFAVGGAQA
G
>Mature_320_residues
SLPLRVAFAGTPEFAASALAAILDAGYSVPLVLTQPDRPAGRGMKLTPSPVKQLALARGLDVDQPEKLRTDEQRQRLAAC
EPDVLVVAAYGLILPAAVLQLPRYGCINIHASLLPRWRGAAPIHRAVEAGDAETGITIMQMDEGLDTGDMLLRRAIPIRP
DDTTGTLHDKLAALGAECIVEALAALPGGTLSRTPQPAAGVTYANKIHRDEAAIDWTRPAADIERAVRAFNPFPVAVGSL
RDTVIKVWAAEVVAVPAPDAAPGTVLAADAEGVVVACGAQALRLRELQRPGSRRFAAGEFLRGFPVCAGERFAVGGAQAG

Specific function: Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by:(I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-

COG id: COG0223

COG function: function code J; Methionyl-tRNA formyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fmt family [H]

Homologues:

Organism=Homo sapiens, GI238814322, Length=315, Percent_Identity=26.6666666666667, Blast_Score=116, Evalue=3e-26,
Organism=Homo sapiens, GI164663775, Length=322, Percent_Identity=31.6770186335404, Blast_Score=114, Evalue=9e-26,
Organism=Homo sapiens, GI21614513, Length=316, Percent_Identity=28.7974683544304, Blast_Score=114, Evalue=1e-25,
Organism=Escherichia coli, GI1789683, Length=303, Percent_Identity=54.1254125412541, Blast_Score=317, Evalue=6e-88,
Organism=Escherichia coli, GI1788589, Length=267, Percent_Identity=31.0861423220974, Blast_Score=128, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI133930964, Length=263, Percent_Identity=24.7148288973384, Blast_Score=87, Evalue=8e-18,
Organism=Drosophila melanogaster, GI45550868, Length=226, Percent_Identity=32.3008849557522, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI28571984, Length=226, Percent_Identity=32.3008849557522, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24585660, Length=249, Percent_Identity=30.1204819277108, Blast_Score=88, Evalue=7e-18,
Organism=Drosophila melanogaster, GI24582400, Length=125, Percent_Identity=34.4, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005794
- InterPro:   IPR005793
- InterPro:   IPR002376
- InterPro:   IPR011034
- InterPro:   IPR001555
- InterPro:   IPR015518 [H]

Pfam domain/function: PF02911 Formyl_trans_C; PF00551 Formyl_trans_N [H]

EC number: =2.1.2.9 [H]

Molecular weight: Translated: 33686; Mature: 33555

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLPLRVAFAGTPEFAASALAAILDAGYSVPLVLTQPDRPAGRGMKLTPSPVKQLALARG
CCCCEEEEECCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHC
LDVDQPEKLRTDEQRQRLAACEPDVLVVAAYGLILPAAVLQLPRYGCINIHASLLPRWRG
CCCCCCHHHCCHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHCCCCCEEEEHHHHCCCCCC
AAPIHRAVEAGDAETGITIMQMDEGLDTGDMLLRRAIPIRPDDTTGTLHDKLAALGAECI
CCHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHH
VEALAALPGGTLSRTPQPAAGVTYANKIHRDEAAIDWTRPAADIERAVRAFNPFPVAVGS
HHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHH
LRDTVIKVWAAEVVAVPAPDAAPGTVLAADAEGVVVACGAQALRLRELQRPGSRRFAAGE
HHHHHHHHHHHHEEEECCCCCCCCCEEEECCCCEEEECCHHHHHHHHHHCCCCCCCHHHH
FLRGFPVCAGERFAVGGAQAG
HHHCCCCCCCCEEECCCCCCC
>Mature Secondary Structure 
SLPLRVAFAGTPEFAASALAAILDAGYSVPLVLTQPDRPAGRGMKLTPSPVKQLALARG
CCCEEEEECCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHC
LDVDQPEKLRTDEQRQRLAACEPDVLVVAAYGLILPAAVLQLPRYGCINIHASLLPRWRG
CCCCCCHHHCCHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHCCCCCEEEEHHHHCCCCCC
AAPIHRAVEAGDAETGITIMQMDEGLDTGDMLLRRAIPIRPDDTTGTLHDKLAALGAECI
CCHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHH
VEALAALPGGTLSRTPQPAAGVTYANKIHRDEAAIDWTRPAADIERAVRAFNPFPVAVGS
HHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHH
LRDTVIKVWAAEVVAVPAPDAAPGTVLAADAEGVVVACGAQALRLRELQRPGSRRFAAGE
HHHHHHHHHHHHEEEECCCCCCCCCEEEECCCCEEEECCHHHHHHHHHHCCCCCCCHHHH
FLRGFPVCAGERFAVGGAQAG
HHHCCCCCCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA