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The map label for this gene is nifF1 [H]

Identifier: 119896306

GI number: 119896306

Start: 18010

End: 18567

Strand: Reverse

Name: nifF1 [H]

Synonym: azo0014

Alternate gene names: 119896306

Gene position: 18567-18010 (Counterclockwise)

Preceding gene: 119896307

Following gene: 119896303

Centisome position: 0.42

GC content: 62.72

Gene sequence:

>558_bases
ATGAACAAGATCGGCATGTTTTTCGGCACCGAAACCGGTACCACCCGCCTCATCGCGAAGAAGATCCACAAGAAGCTGGG
CGACGAGATCGCCGACAAGCCGGTGAATGTGAACCGCATCGAACCGGCGGACATGCTGAAGTACGACGTGCTGATCCTCG
GCACGCCGAGCTACGGCATCGAGGAAGTGCCGGGCCTCAGCGCCGGCTGCCTGGAGCCGAACTGGGCGGAATTCCTGGTG
AAGATGGACAAGCCGGACTTCAGCGGCAAGCGCATCGCGCTGTTCGGGCTGGGCGCGCAGGAGCGTTATTCGGAGCGCTT
CTGCAGCTCTCTGATGCGCATCTACGAAATCTTCAAGGGCTACGGCGCCGAGATCGTCGGCGACGACTGGCCCACCGACG
GCTACACCTTCCAGCAGTCCGCCGCGGTGAAGGACGGCCGCTTCGTCGGGCTGGTGATCGACGAGCGTACCCAGGGGATG
TACACCAACGAGCGCATCGACACCTGGGTGGCAAAGGTGAAGCCGCTGCTGCTGGAGAAGCTCGGGGTCGAGGCCTAA

Upstream 100 bases:

>100_bases
ACCGTCCTCCACCGGCGGACACCTGACCGCGCCGGGCGCCGCGTCTGAGCGCCACGGCGCCGGTGCGGCCTTGAAGATTC
ACTCATCCCAGGTACAGACC

Downstream 100 bases:

>100_bases
GCGGCGTCTGTTCAGCGCCCTTCCCGCTGGGGCCTGGGCGCCTGCCCGCGTTCCTCACCGTTCGCCTTGAGCCTGTCGAA
AGGCGTTCGCGCGCGATGCG

Product: flavodoxin FldA

Products: NA

Alternate protein names: FldB [H]

Number of amino acids: Translated: 185; Mature: 185

Protein sequence:

>185_residues
MNKIGMFFGTETGTTRLIAKKIHKKLGDEIADKPVNVNRIEPADMLKYDVLILGTPSYGIEEVPGLSAGCLEPNWAEFLV
KMDKPDFSGKRIALFGLGAQERYSERFCSSLMRIYEIFKGYGAEIVGDDWPTDGYTFQQSAAVKDGRFVGLVIDERTQGM
YTNERIDTWVAKVKPLLLEKLGVEA

Sequences:

>Translated_185_residues
MNKIGMFFGTETGTTRLIAKKIHKKLGDEIADKPVNVNRIEPADMLKYDVLILGTPSYGIEEVPGLSAGCLEPNWAEFLV
KMDKPDFSGKRIALFGLGAQERYSERFCSSLMRIYEIFKGYGAEIVGDDWPTDGYTFQQSAAVKDGRFVGLVIDERTQGM
YTNERIDTWVAKVKPLLLEKLGVEA
>Mature_185_residues
MNKIGMFFGTETGTTRLIAKKIHKKLGDEIADKPVNVNRIEPADMLKYDVLILGTPSYGIEEVPGLSAGCLEPNWAEFLV
KMDKPDFSGKRIALFGLGAQERYSERFCSSLMRIYEIFKGYGAEIVGDDWPTDGYTFQQSAAVKDGRFVGLVIDERTQGM
YTNERIDTWVAKVKPLLLEKLGVEA

Specific function: Low-potential electron donor to a number of redox enzymes. NifF is the electron donor to nitrogenase [H]

COG id: COG0716

COG function: function code C; Flavodoxins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 flavodoxin-like domain [H]

Homologues:

Organism=Escherichia coli, GI1786900, Length=178, Percent_Identity=41.5730337078652, Blast_Score=128, Evalue=2e-31,
Organism=Escherichia coli, GI1789262, Length=173, Percent_Identity=33.5260115606936, Blast_Score=96, Evalue=2e-21,

Paralogues:

None

Copy number: 242 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001094
- InterPro:   IPR008254
- InterPro:   IPR001226
- InterPro:   IPR010086 [H]

Pfam domain/function: PF00258 Flavodoxin_1 [H]

EC number: NA

Molecular weight: Translated: 20709; Mature: 20709

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS50902 FLAVODOXIN_LIKE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKIGMFFGTETGTTRLIAKKIHKKLGDEIADKPVNVNRIEPADMLKYDVLILGTPSYGI
CCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHEEEEEEEECCCCCH
EEVPGLSAGCLEPNWAEFLVKMDKPDFSGKRIALFGLGAQERYSERFCSSLMRIYEIFKG
HHCCCCCCCCCCCCHHHHEEECCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHC
YGAEIVGDDWPTDGYTFQQSAAVKDGRFVGLVIDERTQGMYTNERIDTWVAKVKPLLLEK
CCCEECCCCCCCCCEEECCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHH
LGVEA
HCCCC
>Mature Secondary Structure
MNKIGMFFGTETGTTRLIAKKIHKKLGDEIADKPVNVNRIEPADMLKYDVLILGTPSYGI
CCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHEEEEEEEECCCCCH
EEVPGLSAGCLEPNWAEFLVKMDKPDFSGKRIALFGLGAQERYSERFCSSLMRIYEIFKG
HHCCCCCCCCCCCCHHHHEEECCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHC
YGAEIVGDDWPTDGYTFQQSAAVKDGRFVGLVIDERTQGMYTNERIDTWVAKVKPLLLEK
CCCEECCCCCCCCCEEECCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHH
LGVEA
HCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1859358 [H]