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The map label for this gene is fdhB1 [H]

Identifier: 119776740

GI number: 119776740

Start: 4246151

End: 4246720

Strand: Reverse

Name: fdhB1 [H]

Synonym: Sama_3608

Alternate gene names: 119776740

Gene position: 4246720-4246151 (Counterclockwise)

Preceding gene: 119776741

Following gene: 119776739

Centisome position: 98.62

GC content: 58.25

Gene sequence:

>570_bases
ATGGCTACAATGAAATTTTTGTGTGACACCAAGCGCTGCATCGAGTGCAACGGTTGCGTCACCGCCTGTAAAAACGAAAA
CGATTCCGCCCTCGAGTGGGGTATCCAGCGCCGCCGCGTGGTCACCATCCGTGATGGTGAGCCGGGTGAAGCCTCCATCT
CGGTTGCTTGCATGCATTGCTCCGATGCCCCCTGCATGGCGGTGTGTCCGGCCAACTGCTTCTACAAAACAGAAGAAGGC
ATAGTGCTGCACGATAAGGACACCTGTATCGGCTGTGGCTACTGTCTGTACGCCTGTCCGTTTGGTGCGCCCCAGTTTCC
CAAAAAGGGTGCCTTCGGCAGCCGCGGTAAAATGGACAAGTGCACCTTCTGTGCCGGTGGCCCGGAAGAAAACTTCTCCG
ATGCCGAGCGCCAGAAGTACGGCAGCAACCGTCTCGCGGAAGGCAAGCTGCCTATGTGTGCTGAGCTGTGTGCCACCAAG
GCGCTGCTCGCCGGTGATGCGGAAGTGGTTTCCAGCATCTTCCGTGAGCGCGTCGCCTATCGTGGTGCCGACAACGGTGC
CTGGAAGTAA

Upstream 100 bases:

>100_bases
GACCTATGGCTACGACCCTGTGACCCAAATGCAGGAAACCAAGTCGAGCCTGTGTCAGATAGTAAAAGCCTGAGCGCGGT
AATCTAGGAGATAAGTCATT

Downstream 100 bases:

>100_bases
CTACAGCCAGGGGGCGGCCTGTTCGCCCCCAACGGAGGTTAACAATGAACAATTGGTTCAAAATCCTGGCCGTGGCTGTG
CTACTCATGCTGGGGATGGG

Product: formate dehydrogenase, iron-sulfur subunit

Products: CO2; NADH

Alternate protein names: NA

Number of amino acids: Translated: 189; Mature: 188

Protein sequence:

>189_residues
MATMKFLCDTKRCIECNGCVTACKNENDSALEWGIQRRRVVTIRDGEPGEASISVACMHCSDAPCMAVCPANCFYKTEEG
IVLHDKDTCIGCGYCLYACPFGAPQFPKKGAFGSRGKMDKCTFCAGGPEENFSDAERQKYGSNRLAEGKLPMCAELCATK
ALLAGDAEVVSSIFRERVAYRGADNGAWK

Sequences:

>Translated_189_residues
MATMKFLCDTKRCIECNGCVTACKNENDSALEWGIQRRRVVTIRDGEPGEASISVACMHCSDAPCMAVCPANCFYKTEEG
IVLHDKDTCIGCGYCLYACPFGAPQFPKKGAFGSRGKMDKCTFCAGGPEENFSDAERQKYGSNRLAEGKLPMCAELCATK
ALLAGDAEVVSSIFRERVAYRGADNGAWK
>Mature_188_residues
ATMKFLCDTKRCIECNGCVTACKNENDSALEWGIQRRRVVTIRDGEPGEASISVACMHCSDAPCMAVCPANCFYKTEEGI
VLHDKDTCIGCGYCLYACPFGAPQFPKKGAFGSRGKMDKCTFCAGGPEENFSDAERQKYGSNRLAEGKLPMCAELCATKA
LLAGDAEVVSSIFRERVAYRGADNGAWK

Specific function: This chain is an electron transfer unit containing 18 cysteine residues, 16 of which occur in four clusters [H]

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787122, Length=171, Percent_Identity=35.0877192982456, Blast_Score=97, Evalue=5e-22,
Organism=Escherichia coli, GI1787872, Length=174, Percent_Identity=35.0574712643678, Blast_Score=97, Evalue=6e-22,
Organism=Escherichia coli, GI1789370, Length=202, Percent_Identity=30.1980198019802, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI226510944, Length=190, Percent_Identity=31.0526315789474, Blast_Score=82, Evalue=2e-17,
Organism=Escherichia coli, GI1790326, Length=201, Percent_Identity=30.3482587064677, Blast_Score=78, Evalue=4e-16,
Organism=Escherichia coli, GI2367345, Length=184, Percent_Identity=31.5217391304348, Blast_Score=77, Evalue=6e-16,
Organism=Escherichia coli, GI1787749, Length=197, Percent_Identity=28.4263959390863, Blast_Score=73, Evalue=1e-14,
Organism=Escherichia coli, GI1788811, Length=126, Percent_Identity=30.1587301587302, Blast_Score=63, Evalue=1e-11,
Organism=Escherichia coli, GI2367245, Length=171, Percent_Identity=30.4093567251462, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI87082180, Length=102, Percent_Identity=36.2745098039216, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI87082114, Length=91, Percent_Identity=34.0659340659341, Blast_Score=60, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR000813 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: 1.2.1.2

Molecular weight: Translated: 20491; Mature: 20359

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

9.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
12.7 %Cys+Met (Translated Protein)
9.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
12.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATMKFLCDTKRCIECNGCVTACKNENDSALEWGIQRRRVVTIRDGEPGEASISVACMHC
CCCHHHHHCCCCEEECCCCEEECCCCCCHHHHHCCCCCEEEEEECCCCCCCEEEEEEEEC
SDAPCMAVCPANCFYKTEEGIVLHDKDTCIGCGYCLYACPFGAPQFPKKGAFGSRGKMDK
CCCCEEEECCCCCEEECCCCEEEECCCCEEECCHHHHHCCCCCCCCCCCCCCCCCCCCCC
CTFCAGGPEENFSDAERQKYGSNRLAEGKLPMCAELCATKALLAGDAEVVSSIFRERVAY
EEEECCCCCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
RGADNGAWK
CCCCCCCCC
>Mature Secondary Structure 
ATMKFLCDTKRCIECNGCVTACKNENDSALEWGIQRRRVVTIRDGEPGEASISVACMHC
CCHHHHHCCCCEEECCCCEEECCCCCCHHHHHCCCCCEEEEEECCCCCCCEEEEEEEEC
SDAPCMAVCPANCFYKTEEGIVLHDKDTCIGCGYCLYACPFGAPQFPKKGAFGSRGKMDK
CCCCEEEECCCCCEEECCCCEEEECCCCEEECCHHHHHCCCCCCCCCCCCCCCCCCCCCC
CTFCAGGPEENFSDAERQKYGSNRLAEGKLPMCAELCATKALLAGDAEVVSSIFRERVAY
EEEECCCCCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
RGADNGAWK
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1781728; 14500908 [H]