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The map label for this gene is phsA [H]

Identifier: 119776309

GI number: 119776309

Start: 3765105

End: 3767387

Strand: Reverse

Name: phsA [H]

Synonym: Sama_3177

Alternate gene names: 119776309

Gene position: 3767387-3765105 (Counterclockwise)

Preceding gene: 119776314

Following gene: 119776308

Centisome position: 87.49

GC content: 58.78

Gene sequence:

>2283_bases
ATGATAGAGCTCTGCAGGCGCGACTTTCTCAAAGGTGCAGGTGCGGCCGGGGCGGGGTGTGCCCTGGCCACAGCGCTGCC
TGGGACGCTGGCGGCCATGGGGGCCCAACCCCTCAGTGGCAGCGAAAAAGCCATTCCGAGTATTTGTGAAATGTGCTCCA
CCCGTTGCCCGATTTCGGCAAGGGTTTTGGATGGCAAGGCCGTGTATATCGAAGGCAACGCAGCAGCCAAGTCCTTTGGC
GGCAAGGTATGTGCCCGGGGCGGTGCCGGCCACAGCTTGCTGTATGACCCACAGCGGCTGGTAAAACCCCTGAAACGCGT
CGGCGAGCGCGGTGAAGGTAAGTGGCAGGAAATCGAATGGAGCGAAGCCTACGGCATTCTTGCCCAGCGGCTCGAACAAA
TCCGCCGCGACCATGGCGCCGAAACCGTGGCCTTTTCATCCAAATCGGGCTCGCTGTCGGGGCATCTGTTCCACTTTGCC
AAGGCCTTTGGCAGCCCCAATACGTTTACTCACGCAACCACCTGCCCCGGCGCCTATGTGGTGGCCGCTAAGGCCATGTT
TGGCGGAAAGGTGAAGCGGGATTTGGGTAATTCCAAATACATCATCAACTTCGGCCATAACCTTTACGAAGGCATCAATA
TGTCCGAAACCCGCGCCATGATGAAGGCGCAAACCGACAAGCGCGCCAAGCTGGTGGTGTTTGAGCCGCGCTTCTCCATC
GTGGCCGATAAAGCCGATGAGTGGTATGCCATCCGTCCCGGCTCTGATGTGGCCGTGGCGCTGGCGCTGTGCCATGTGCT
GATTAGCGAGAACCTCTACGACAAGGCCTTTATCGAGCGTTACGTGGAAGGCTTCGAGGCCTTTGCCGCCGAAGTCAGCG
CCTATACCCCCGAGTGGGCAGAAACCATTTCCGATGTGCCCGCCCAAGACATTCGCCGCATTGCCCGCGAATTTGCCAAG
GCCGCGCCCCATGCGGTGGTGGACTTTGGCCACCGTGCCACCTTCACCCCGGAAGAGTTTGATATGCGCCGGGCCCTGTA
CGCGGCCAACGTACTGATTGGCAACTTTGAGCGCAAGGGCGGTATCTACTTTGGCCAGAAGGCGGCCGGGTACAACAAGC
TTGCAGGAGAATCCGTGGCGCCTGTGTTGGGTAAGCCCGGCGTGGCCGACATGCCCAAGCCCACAGCCAAGCGTATCGAC
CAGATGGATGAGCAGTACGCCATGATGTGGAGCGAGGGCGGCATTTATCAGTCGGTTATCGATGCCACCCTGGAGGCCAA
ACCCTACCAGCTGCGCGGCTGGGTAATGTGCCGTACCAACCCGATGCAAACCATGACTGACCGCGCCAAGGTGGTTGAGT
CGCTGAAAAAGCTCGATTTTGTGGCCGTGGTGGACGTGTATATCAGCGAAACCGCCGCCTGGGCCGATTTGATTTTGCCC
GAGTCCACCTACCTTGAGCGCGATGAGGAGATCAGCGACAAGTCCGGCAAGAGCCCCGGCTACTACGTGCGTCAGCGGGT
GGTGGACGTGATTGGCGATACCAAGCCGTCATGGCAAATCTTCCGCGAGCTGGGTCTTAAGATGGGCATGGACAAGTACT
ACCCCTGGGAGACCATGGAAACCCTGCAACTGCTGCAGGCCAACAAGGATGTGGCGCTTTTGAATCACATCAAGGAAAAG
GGCTTTGTCAGCTATGGCAAGCCGTTGATGCTGCGTGAAAAATCCATGGTGCAGGAGTTTGTGACTGCCTATCCAGGCGC
CCATGTCGCGGATGAGGACGGCAGTTACGCCAGCCTGCTCAAGTTCAAAACCCCCAGCGGCAAGATAGAACTGGTGAGCC
CCAAGGTGGAAGCCATGGCCCCGGGCCGCGGCGGCATCAAGTACCGGCCCGTGACCTTAAAACAGCCCAATGAGCTCTAC
TTCATTCAGGGTAAGGTGGCGGTGCACACCAATGGTGCCACCCACAATATCCCTATGCTCGCCAATCTGATGTCGGACAA
CGCCGTGTGGGTGCATCCCAGCACAGCCGAGGCGCTGGGTATCAAGGATGGTGATGCTATTCGGCTTAAATCCAGCGTGG
GCGTGGAAGAAGGCAAGGCGCTCGTCACTAAGGGCATTCGCCCGGATACCCTGTTTGCTTACATGGGCTTTGGCTCGAAA
AACCGTGAGCTGGTGCGTGCCTCCGGCAAGGGGATCCACTGTGGAAACCTGCTGCCCAATATGACGGCGCCTGTCTGCGG
CATGAACGTGCACACCACGGGTGTGATCCTGGAAAAGGCATAA

Upstream 100 bases:

>100_bases
GCCCTGCCGACAATGGCATTAGCCGGTGAATGCCCGTCATTCGCCACAGAAGAACGATAACGCTAATCCCATTCTGCCCT
TTGGGCATTTGGAGGAAACC

Downstream 100 bases:

>100_bases
GGGGGCCCGGACTATGAGCAAAAGATACGTAATGGTGCACGACGAGAACAAGTGCATCGGCTGTCAGGCCTGTAGCGTGG
CTTGCCGCAGCATTAATCAG

Product: polysulfide reductase subunit A

Products: Reduced form of N-Oxide compounds [C]

Alternate protein names: NA

Number of amino acids: Translated: 760; Mature: 760

Protein sequence:

>760_residues
MIELCRRDFLKGAGAAGAGCALATALPGTLAAMGAQPLSGSEKAIPSICEMCSTRCPISARVLDGKAVYIEGNAAAKSFG
GKVCARGGAGHSLLYDPQRLVKPLKRVGERGEGKWQEIEWSEAYGILAQRLEQIRRDHGAETVAFSSKSGSLSGHLFHFA
KAFGSPNTFTHATTCPGAYVVAAKAMFGGKVKRDLGNSKYIINFGHNLYEGINMSETRAMMKAQTDKRAKLVVFEPRFSI
VADKADEWYAIRPGSDVAVALALCHVLISENLYDKAFIERYVEGFEAFAAEVSAYTPEWAETISDVPAQDIRRIAREFAK
AAPHAVVDFGHRATFTPEEFDMRRALYAANVLIGNFERKGGIYFGQKAAGYNKLAGESVAPVLGKPGVADMPKPTAKRID
QMDEQYAMMWSEGGIYQSVIDATLEAKPYQLRGWVMCRTNPMQTMTDRAKVVESLKKLDFVAVVDVYISETAAWADLILP
ESTYLERDEEISDKSGKSPGYYVRQRVVDVIGDTKPSWQIFRELGLKMGMDKYYPWETMETLQLLQANKDVALLNHIKEK
GFVSYGKPLMLREKSMVQEFVTAYPGAHVADEDGSYASLLKFKTPSGKIELVSPKVEAMAPGRGGIKYRPVTLKQPNELY
FIQGKVAVHTNGATHNIPMLANLMSDNAVWVHPSTAEALGIKDGDAIRLKSSVGVEEGKALVTKGIRPDTLFAYMGFGSK
NRELVRASGKGIHCGNLLPNMTAPVCGMNVHTTGVILEKA

Sequences:

>Translated_760_residues
MIELCRRDFLKGAGAAGAGCALATALPGTLAAMGAQPLSGSEKAIPSICEMCSTRCPISARVLDGKAVYIEGNAAAKSFG
GKVCARGGAGHSLLYDPQRLVKPLKRVGERGEGKWQEIEWSEAYGILAQRLEQIRRDHGAETVAFSSKSGSLSGHLFHFA
KAFGSPNTFTHATTCPGAYVVAAKAMFGGKVKRDLGNSKYIINFGHNLYEGINMSETRAMMKAQTDKRAKLVVFEPRFSI
VADKADEWYAIRPGSDVAVALALCHVLISENLYDKAFIERYVEGFEAFAAEVSAYTPEWAETISDVPAQDIRRIAREFAK
AAPHAVVDFGHRATFTPEEFDMRRALYAANVLIGNFERKGGIYFGQKAAGYNKLAGESVAPVLGKPGVADMPKPTAKRID
QMDEQYAMMWSEGGIYQSVIDATLEAKPYQLRGWVMCRTNPMQTMTDRAKVVESLKKLDFVAVVDVYISETAAWADLILP
ESTYLERDEEISDKSGKSPGYYVRQRVVDVIGDTKPSWQIFRELGLKMGMDKYYPWETMETLQLLQANKDVALLNHIKEK
GFVSYGKPLMLREKSMVQEFVTAYPGAHVADEDGSYASLLKFKTPSGKIELVSPKVEAMAPGRGGIKYRPVTLKQPNELY
FIQGKVAVHTNGATHNIPMLANLMSDNAVWVHPSTAEALGIKDGDAIRLKSSVGVEEGKALVTKGIRPDTLFAYMGFGSK
NRELVRASGKGIHCGNLLPNMTAPVCGMNVHTTGVILEKA
>Mature_760_residues
MIELCRRDFLKGAGAAGAGCALATALPGTLAAMGAQPLSGSEKAIPSICEMCSTRCPISARVLDGKAVYIEGNAAAKSFG
GKVCARGGAGHSLLYDPQRLVKPLKRVGERGEGKWQEIEWSEAYGILAQRLEQIRRDHGAETVAFSSKSGSLSGHLFHFA
KAFGSPNTFTHATTCPGAYVVAAKAMFGGKVKRDLGNSKYIINFGHNLYEGINMSETRAMMKAQTDKRAKLVVFEPRFSI
VADKADEWYAIRPGSDVAVALALCHVLISENLYDKAFIERYVEGFEAFAAEVSAYTPEWAETISDVPAQDIRRIAREFAK
AAPHAVVDFGHRATFTPEEFDMRRALYAANVLIGNFERKGGIYFGQKAAGYNKLAGESVAPVLGKPGVADMPKPTAKRID
QMDEQYAMMWSEGGIYQSVIDATLEAKPYQLRGWVMCRTNPMQTMTDRAKVVESLKKLDFVAVVDVYISETAAWADLILP
ESTYLERDEEISDKSGKSPGYYVRQRVVDVIGDTKPSWQIFRELGLKMGMDKYYPWETMETLQLLQANKDVALLNHIKEK
GFVSYGKPLMLREKSMVQEFVTAYPGAHVADEDGSYASLLKFKTPSGKIELVSPKVEAMAPGRGGIKYRPVTLKQPNELY
FIQGKVAVHTNGATHNIPMLANLMSDNAVWVHPSTAEALGIKDGDAIRLKSSVGVEEGKALVTKGIRPDTLFAYMGFGSK
NRELVRASGKGIHCGNLLPNMTAPVCGMNVHTTGVILEKA

Specific function: Oxidoreductase which produces hydrogen sulfide from thiosulfate (Potential) [H]

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Cytoplasm Face Of The Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI87081797, Length=800, Percent_Identity=25.25, Blast_Score=200, Evalue=2e-52,
Organism=Escherichia coli, GI1787870, Length=849, Percent_Identity=26.5017667844523, Blast_Score=196, Evalue=6e-51,
Organism=Escherichia coli, GI171474008, Length=779, Percent_Identity=26.0590500641849, Blast_Score=192, Evalue=9e-50,
Organism=Escherichia coli, GI3868721, Length=705, Percent_Identity=26.5248226950355, Blast_Score=172, Evalue=1e-43,
Organism=Escherichia coli, GI1787231, Length=682, Percent_Identity=21.9941348973607, Blast_Score=109, Evalue=8e-25,
Organism=Escherichia coli, GI145693196, Length=676, Percent_Identity=22.189349112426, Blast_Score=100, Evalue=6e-22,
Organism=Escherichia coli, GI1788534, Length=819, Percent_Identity=21.001221001221, Blast_Score=88, Evalue=2e-18,
Organism=Escherichia coli, GI87081994, Length=793, Percent_Identity=21.9419924337957, Blast_Score=86, Evalue=7e-18,
Organism=Escherichia coli, GI3868719, Length=598, Percent_Identity=22.742474916388, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI3868720, Length=597, Percent_Identity=21.7755443886097, Blast_Score=81, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655
- InterPro:   IPR006311 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: 1.8.99.- [C]

Molecular weight: Translated: 83067; Mature: 83067

Theoretical pI: Translated: 8.63; Mature: 8.63

Prosite motif: PS00551 MOLYBDOPTERIN_PROK_1 ; PS00490 MOLYBDOPTERIN_PROK_2 ; PS00932 MOLYBDOPTERIN_PROK_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIELCRRDFLKGAGAAGAGCALATALPGTLAAMGAQPLSGSEKAIPSICEMCSTRCPISA
CHHHHHHHHHCCCCCCCCCHHHHHHCCHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCEE
RVLDGKAVYIEGNAAAKSFGGKVCARGGAGHSLLYDPQRLVKPLKRVGERGEGKWQEIEW
EEECCEEEEEECCHHHHHCCCCEEECCCCCCCCEECHHHHHHHHHHHCCCCCCCCEECCH
SEAYGILAQRLEQIRRDHGAETVAFSSKSGSLSGHLFHFAKAFGSPNTFTHATTCPGAYV
HHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCCEECCCCCHHH
VAAKAMFGGKVKRDLGNSKYIINFGHNLYEGINMSETRAMMKAQTDKRAKLVVFEPRFSI
HHHHHHHCCHHHHHCCCCEEEEECCCHHHHCCCHHHHHHHHHHCCCCCEEEEEECCCCEE
VADKADEWYAIRPGSDVAVALALCHVLISENLYDKAFIERYVEGFEAFAAEVSAYTPEWA
EECCCCCEEEECCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHH
ETISDVPAQDIRRIAREFAKAAPHAVVDFGHRATFTPEEFDMRRALYAANVLIGNFERKG
HHHHCCCHHHHHHHHHHHHHHCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHEECCCCCC
GIYFGQKAAGYNKLAGESVAPVLGKPGVADMPKPTAKRIDQMDEQYAMMWSEGGIYQSVI
CEEECCCCCCCCHHCCCCCCHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHH
DATLEAKPYQLRGWVMCRTNPMQTMTDRAKVVESLKKLDFVAVVDVYISETAAWADLILP
HHHHCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCC
ESTYLERDEEISDKSGKSPGYYVRQRVVDVIGDTKPSWQIFRELGLKMGMDKYYPWETME
CHHHHCCCHHHCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCHHHH
TLQLLQANKDVALLNHIKEKGFVSYGKPLMLREKSMVQEFVTAYPGAHVADEDGSYASLL
HHHHHHCCCCHHHHHHHHHCCCCCCCCCCEEHHHHHHHHHHHHCCCCCCCCCCCCEEEEE
KFKTPSGKIELVSPKVEAMAPGRGGIKYRPVTLKQPNELYFIQGKVAVHTNGATHNIPML
EEECCCCCEEEECCCHHEECCCCCCCEEEEEEECCCCEEEEEEEEEEEEECCCCCCCHHH
ANLMSDNAVWVHPSTAEALGIKDGDAIRLKSSVGVEEGKALVTKGIRPDTLFAYMGFGSK
HHHHCCCEEEECCCCHHHCCCCCCCEEEEECCCCCCCCCHHHHCCCCCCCEEEEECCCCC
NRELVRASGKGIHCGNLLPNMTAPVCGMNVHTTGVILEKA
CCHHEEECCCCEEECCCCCCCCCCCCCCCEEEEEEEEECC
>Mature Secondary Structure
MIELCRRDFLKGAGAAGAGCALATALPGTLAAMGAQPLSGSEKAIPSICEMCSTRCPISA
CHHHHHHHHHCCCCCCCCCHHHHHHCCHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCEE
RVLDGKAVYIEGNAAAKSFGGKVCARGGAGHSLLYDPQRLVKPLKRVGERGEGKWQEIEW
EEECCEEEEEECCHHHHHCCCCEEECCCCCCCCEECHHHHHHHHHHHCCCCCCCCEECCH
SEAYGILAQRLEQIRRDHGAETVAFSSKSGSLSGHLFHFAKAFGSPNTFTHATTCPGAYV
HHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCCEECCCCCHHH
VAAKAMFGGKVKRDLGNSKYIINFGHNLYEGINMSETRAMMKAQTDKRAKLVVFEPRFSI
HHHHHHHCCHHHHHCCCCEEEEECCCHHHHCCCHHHHHHHHHHCCCCCEEEEEECCCCEE
VADKADEWYAIRPGSDVAVALALCHVLISENLYDKAFIERYVEGFEAFAAEVSAYTPEWA
EECCCCCEEEECCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHH
ETISDVPAQDIRRIAREFAKAAPHAVVDFGHRATFTPEEFDMRRALYAANVLIGNFERKG
HHHHCCCHHHHHHHHHHHHHHCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHEECCCCCC
GIYFGQKAAGYNKLAGESVAPVLGKPGVADMPKPTAKRIDQMDEQYAMMWSEGGIYQSVI
CEEECCCCCCCCHHCCCCCCHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHH
DATLEAKPYQLRGWVMCRTNPMQTMTDRAKVVESLKKLDFVAVVDVYISETAAWADLILP
HHHHCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCC
ESTYLERDEEISDKSGKSPGYYVRQRVVDVIGDTKPSWQIFRELGLKMGMDKYYPWETME
CHHHHCCCHHHCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCHHHH
TLQLLQANKDVALLNHIKEKGFVSYGKPLMLREKSMVQEFVTAYPGAHVADEDGSYASLL
HHHHHHCCCCHHHHHHHHHCCCCCCCCCCEEHHHHHHHHHHHHCCCCCCCCCCCCEEEEE
KFKTPSGKIELVSPKVEAMAPGRGGIKYRPVTLKQPNELYFIQGKVAVHTNGATHNIPML
EEECCCCCEEEECCCHHEECCCCCCCEEEEEEECCCCEEEEEEEEEEEEECCCCCCCHHH
ANLMSDNAVWVHPSTAEALGIKDGDAIRLKSSVGVEEGKALVTKGIRPDTLFAYMGFGSK
HHHHCCCEEEECCCCHHHCCCCCCCEEEEECCCCCCCCCHHHHCCCCCCCEEEEECCCCC
NRELVRASGKGIHCGNLLPNMTAPVCGMNVHTTGVILEKA
CCHHEEECCCCEEECCCCCCCCCCCCCCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: May Bind 4Fe-4S Cluster. [C]

Metal ions: Fe; Mo [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: N-Oxide; Sulfoxide Compounds Including Trimethylamine N-Oxide. [C]

Specific reaction: Reduces Various N-Oxide And Sulfoxide Compounds Including Trimethylamine N-Oxide. [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7751291; 11677609; 7737516 [H]