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The map label for this gene is psrB [H]

Identifier: 119776308

GI number: 119776308

Start: 3764525

End: 3765091

Strand: Reverse

Name: psrB [H]

Synonym: Sama_3176

Alternate gene names: 119776308

Gene position: 3765091-3764525 (Counterclockwise)

Preceding gene: 119776309

Following gene: 119776307

Centisome position: 87.44

GC content: 57.67

Gene sequence:

>567_bases
ATGAGCAAAAGATACGTAATGGTGCACGACGAGAACAAGTGCATCGGCTGTCAGGCCTGTAGCGTGGCTTGCCGCAGCAT
TAATCAGGTACCCGATGGCGTAAGCCGCTTGCAGGTGCGCATCGAAGGCCCCTTTGGTGAGGCGCCCCATTTGCACTTCA
AGTACAACCGGGTGTCGTGCCAGCAGTGTGAGGATGCGCCCTGCGTCAAGGTGTGTCCGACCGGCGCCGCTTACGTTGGT
GACGATGGCATTGTGTCCATCAAGGCCGAGAAGTGCGTGGGCTGCATGTACTGCGTGGCCGCCTGCCCCTACAAGGTGCG
CTTTATGAACCCTGAAACCAGGGTGGCGGACAAGTGCAACTTCTGTAAGGAAACCCGTCTTGCCCGGGGTGAACAGCCCG
CCTGTGTCACTGTATGCCCAACAGATGCGCTCTGTTTCGGTGATGCAGCTGACCCTGCCAGCGAGGTGTCCAAACTGCTT
GCCACCAAGGTGAGTTATCAGGACAAGACACATCTTGGCACCAAACCCAGGCTGTACCGTATTCCAGCCAAGCGTGGAGG
TATCTGA

Upstream 100 bases:

>100_bases
CACTGTGGAAACCTGCTGCCCAATATGACGGCGCCTGTCTGCGGCATGAACGTGCACACCACGGGTGTGATCCTGGAAAA
GGCATAAGGGGGCCCGGACT

Downstream 100 bases:

>100_bases
TTATGAACAATATCTGGGGTGATATGAGTCAGTACGACGGCATTACCTGGCATTGGGTGATTGCGGTGTACCTCTTTATG
GCCGGTTTGTCGGCGGGCAG

Product: polysulfide reductase subunit B

Products: oxidized cytochrome C552; NH3 [C]

Alternate protein names: Sulfur reductase chain B [H]

Number of amino acids: Translated: 188; Mature: 187

Protein sequence:

>188_residues
MSKRYVMVHDENKCIGCQACSVACRSINQVPDGVSRLQVRIEGPFGEAPHLHFKYNRVSCQQCEDAPCVKVCPTGAAYVG
DDGIVSIKAEKCVGCMYCVAACPYKVRFMNPETRVADKCNFCKETRLARGEQPACVTVCPTDALCFGDAADPASEVSKLL
ATKVSYQDKTHLGTKPRLYRIPAKRGGI

Sequences:

>Translated_188_residues
MSKRYVMVHDENKCIGCQACSVACRSINQVPDGVSRLQVRIEGPFGEAPHLHFKYNRVSCQQCEDAPCVKVCPTGAAYVG
DDGIVSIKAEKCVGCMYCVAACPYKVRFMNPETRVADKCNFCKETRLARGEQPACVTVCPTDALCFGDAADPASEVSKLL
ATKVSYQDKTHLGTKPRLYRIPAKRGGI
>Mature_187_residues
SKRYVMVHDENKCIGCQACSVACRSINQVPDGVSRLQVRIEGPFGEAPHLHFKYNRVSCQQCEDAPCVKVCPTGAAYVGD
DGIVSIKAEKCVGCMYCVAACPYKVRFMNPETRVADKCNFCKETRLARGEQPACVTVCPTDALCFGDAADPASEVSKLLA
TKVSYQDKTHLGTKPRLYRIPAKRGGI

Specific function: Component of the phosphorylative electron transport system with polysulfide as the terminal acceptor [H]

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI2367345, Length=186, Percent_Identity=55.3763440860215, Blast_Score=216, Evalue=6e-58,
Organism=Escherichia coli, GI226510944, Length=177, Percent_Identity=46.8926553672316, Blast_Score=174, Evalue=3e-45,
Organism=Escherichia coli, GI1790326, Length=194, Percent_Identity=35.0515463917526, Blast_Score=100, Evalue=5e-23,
Organism=Escherichia coli, GI1787872, Length=189, Percent_Identity=33.8624338624339, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1787749, Length=180, Percent_Identity=32.7777777777778, Blast_Score=98, Evalue=3e-22,
Organism=Escherichia coli, GI1787122, Length=165, Percent_Identity=35.1515151515151, Blast_Score=96, Evalue=1e-21,
Organism=Escherichia coli, GI1789370, Length=209, Percent_Identity=30.622009569378, Blast_Score=82, Evalue=2e-17,
Organism=Escherichia coli, GI1789067, Length=158, Percent_Identity=36.7088607594937, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI87082179, Length=145, Percent_Identity=34.4827586206897, Blast_Score=77, Evalue=8e-16,
Organism=Escherichia coli, GI1788811, Length=180, Percent_Identity=32.2222222222222, Blast_Score=75, Evalue=2e-15,
Organism=Escherichia coli, GI87082180, Length=167, Percent_Identity=35.3293413173653, Blast_Score=74, Evalue=5e-15,
Organism=Escherichia coli, GI1787963, Length=149, Percent_Identity=27.5167785234899, Blast_Score=72, Evalue=2e-14,
Organism=Escherichia coli, GI2367245, Length=149, Percent_Identity=35.5704697986577, Blast_Score=70, Evalue=8e-14,
Organism=Escherichia coli, GI1787740, Length=96, Percent_Identity=34.375, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 20532; Mature: 20401

Theoretical pI: Translated: 8.30; Mature: 8.30

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

9.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
11.2 %Cys+Met (Translated Protein)
9.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
10.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKRYVMVHDENKCIGCQACSVACRSINQVPDGVSRLQVRIEGPFGEAPHLHFKYNRVSC
CCCCEEEEECCCCEECHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCEEEEEECCCCC
QQCEDAPCVKVCPTGAAYVGDDGIVSIKAEKCVGCMYCVAACPYKVRFMNPETRVADKCN
CCCCCCCCEEECCCCCEEECCCCEEEEEHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHH
FCKETRLARGEQPACVTVCPTDALCFGDAADPASEVSKLLATKVSYQDKTHLGTKPRLYR
HHHHHHHCCCCCCCEEEECCCCCEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEE
IPAKRGGI
CCCCCCCC
>Mature Secondary Structure 
SKRYVMVHDENKCIGCQACSVACRSINQVPDGVSRLQVRIEGPFGEAPHLHFKYNRVSC
CCCEEEEECCCCEECHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCEEEEEECCCCC
QQCEDAPCVKVCPTGAAYVGDDGIVSIKAEKCVGCMYCVAACPYKVRFMNPETRVADKCN
CCCCCCCCEEECCCCCEEECCCCEEEEEHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHH
FCKETRLARGEQPACVTVCPTDALCFGDAADPASEVSKLLATKVSYQDKTHLGTKPRLYR
HHHHHHHCCCCCCCEEEECCCCCEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEE
IPAKRGGI
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced cytochrome C552; nitrite [C]

Specific reaction: reduced cytochrome C552 + nitrite = oxidized cytochrome C552 + NH3 [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1597189; 14500908 [H]