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The map label for this gene is mlaE [H]

Identifier: 119776212

GI number: 119776212

Start: 3657277

End: 3658059

Strand: Reverse

Name: mlaE [H]

Synonym: Sama_3080

Alternate gene names: 119776212

Gene position: 3658059-3657277 (Counterclockwise)

Preceding gene: 119776213

Following gene: 119776211

Centisome position: 84.95

GC content: 55.17

Gene sequence:

>783_bases
GTGGCGCTAATTGATGCAGTTGCACGCCTGGGGCGTGGCGCCATAAATCAGGTGGCCGGCATGGGCCGGGCGGGCGTGAT
GTTGTGGGGCGCCATCTTTTCCGTCCCCAAACCCCAAAAAGGTTTTCCGCTGCTGGTAAAGCAGCTTTATGTGGTCGGTG
TGCAGTCGCTGATCATTATTCTGGTGTCAGGCCTCTTTATCGGCATGGTATTGGCGCTGCAGGGTTATACCATTCTGGTG
GATTTTGGTACCGAAGAAAGTCTGGGCCCTATGGTGGCGCTGAGTTTGCTGCGGGAGCTGGGGCCTGTGGTCACAGCATT
GCTGTTTGCCGGAAGGGCAGGGTCGGCGCTGACTGCTGAGATTGGCCTGATGAAGAGCACAGAGCAGCTCTCAAGCCTTG
AAATGATGGCCGTTGATCCGCTGCGGCAAATTATCGCTCCCCGATTCTGGGCCGGTGTTATCAGTATGCCGCTGTTGGCG
CTGATGTTTACCGCTGTGGGTATTTGGGGCGGTCATATCGTGGGCGTAGAGTGGAAAGGCATCGACAATGGCGCCTTCTG
GTCGATTTTACAGGCGTCGGTTGAGTGGCGTCAGGACATAGTAAACTGTTTGATTAAAAGCCTGGTGTTTGCCGTAGTGG
TGACCTGGATTGCTCTGTACCGCGGTTACGAAGTGGTGCCCAACCCGGAAGGGATAAGTAAGGCCACTACTTCTACCGTG
GTGCAATCGAGTCTTGCCGTACTGGCATTGGATTTTTTGCTGACCGCCCTCATGTTTGGCTAA

Upstream 100 bases:

>100_bases
TCATCCCATGACCCCAGATTGCGGCAGTTTATCGGCGGCGAACCCGATGGTCCTGTGCCTTTTCATTATCCTGCCAACGA
TTATATGAAGGAGTTAACCG

Downstream 100 bases:

>100_bases
TGCCAGGCGTTCAGCTTGCTGCAAGGAGTGAAACTTTAGCATGTTGTCACGGAAAGTTGAGATTTGGGTTGGTTTGTTCC
TGCTTGCGGGCATCGCCGCC

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 260; Mature: 259

Protein sequence:

>260_residues
MALIDAVARLGRGAINQVAGMGRAGVMLWGAIFSVPKPQKGFPLLVKQLYVVGVQSLIIILVSGLFIGMVLALQGYTILV
DFGTEESLGPMVALSLLRELGPVVTALLFAGRAGSALTAEIGLMKSTEQLSSLEMMAVDPLRQIIAPRFWAGVISMPLLA
LMFTAVGIWGGHIVGVEWKGIDNGAFWSILQASVEWRQDIVNCLIKSLVFAVVVTWIALYRGYEVVPNPEGISKATTSTV
VQSSLAVLALDFLLTALMFG

Sequences:

>Translated_260_residues
MALIDAVARLGRGAINQVAGMGRAGVMLWGAIFSVPKPQKGFPLLVKQLYVVGVQSLIIILVSGLFIGMVLALQGYTILV
DFGTEESLGPMVALSLLRELGPVVTALLFAGRAGSALTAEIGLMKSTEQLSSLEMMAVDPLRQIIAPRFWAGVISMPLLA
LMFTAVGIWGGHIVGVEWKGIDNGAFWSILQASVEWRQDIVNCLIKSLVFAVVVTWIALYRGYEVVPNPEGISKATTSTV
VQSSLAVLALDFLLTALMFG
>Mature_259_residues
ALIDAVARLGRGAINQVAGMGRAGVMLWGAIFSVPKPQKGFPLLVKQLYVVGVQSLIIILVSGLFIGMVLALQGYTILVD
FGTEESLGPMVALSLLRELGPVVTALLFAGRAGSALTAEIGLMKSTEQLSSLEMMAVDPLRQIIAPRFWAGVISMPLLAL
MFTAVGIWGGHIVGVEWKGIDNGAFWSILQASVEWRQDIVNCLIKSLVFAVVVTWIALYRGYEVVPNPEGISKATTSTVV
QSSLAVLALDFLLTALMFG

Specific function: Part of the ABC transporter complex mlaFEDB that actively prevents phospholipid accumulation at the cell surface. Probably maintains lipid asymmetry in the outer membrane by retrograde trafficking of phospholipids from the outer membrane to the inner memb

COG id: COG0767

COG function: function code Q; ABC-type transport system involved in resistance to organic solvents, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mlaE permease family [H]

Homologues:

Organism=Escherichia coli, GI1789585, Length=258, Percent_Identity=67.4418604651163, Blast_Score=354, Evalue=3e-99,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003453 [H]

Pfam domain/function: PF02405 DUF140 [H]

EC number: NA

Molecular weight: Translated: 27747; Mature: 27615

Theoretical pI: Translated: 7.35; Mature: 7.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALIDAVARLGRGAINQVAGMGRAGVMLWGAIFSVPKPQKGFPLLVKQLYVVGVQSLIII
CHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
LVSGLFIGMVLALQGYTILVDFGTEESLGPMVALSLLRELGPVVTALLFAGRAGSALTAE
HHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
IGLMKSTEQLSSLEMMAVDPLRQIIAPRFWAGVISMPLLALMFTAVGIWGGHIVGVEWKG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECC
IDNGAFWSILQASVEWRQDIVNCLIKSLVFAVVVTWIALYRGYEVVPNPEGISKATTSTV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHH
VQSSLAVLALDFLLTALMFG
HHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ALIDAVARLGRGAINQVAGMGRAGVMLWGAIFSVPKPQKGFPLLVKQLYVVGVQSLIII
HHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
LVSGLFIGMVLALQGYTILVDFGTEESLGPMVALSLLRELGPVVTALLFAGRAGSALTAE
HHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
IGLMKSTEQLSSLEMMAVDPLRQIIAPRFWAGVISMPLLALMFTAVGIWGGHIVGVEWKG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECC
IDNGAFWSILQASVEWRQDIVNCLIKSLVFAVVVTWIALYRGYEVVPNPEGISKATTSTV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHH
VQSSLAVLALDFLLTALMFG
HHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]