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The map label for this gene is miaA

Identifier: 119776158

GI number: 119776158

Start: 3600050

End: 3600970

Strand: Reverse

Name: miaA

Synonym: Sama_3026

Alternate gene names: 119776158

Gene position: 3600970-3600050 (Counterclockwise)

Preceding gene: 119776159

Following gene: 119776157

Centisome position: 83.62

GC content: 54.07

Gene sequence:

>921_bases
ATGACTTCCCTGCCCAAGGTGCTGTTTCTAATGGGGCCAACGGCTTCAGGCAAGACGGCGCTGGCGCTGGACATGGCTGA
ACATCACAACTGTGAGATTATTTCGGTCGACTCGGCGCTTATCTATCGTGGCATGGATATAGGGACCGCCAAACCCACAG
CTTCGGAGCTGGCGAGGGCGCCCCACAAGCTGATAGATATCCTGGACCCGCTCGAGAGTTACTCGGCGGCAGATTTCAGA
GCCGATGCTGTCAGGGAGATAGAAGAGACGCTATCCCGTGGTAAAACCCCGCTGCTTGTTGGGGGGACCATGATGTATTT
CAAGACGTTACTCGATGGTTTATCACCTTTACCCAGTGCAGATGACGCCGTGAGGGCTCAAATTGCTGCCGAGGTTGAGG
CGCGGGGATGGCAGGCGCTCCATGACGAATTACGCAACATAGATCCTGTCTCTGCCGAGCGTATTCATCCCAATGATCCC
CAGCGCTTGTCGCGGGCCATTGAAGTGTACCGAATAAGTGGCAAGACACTGACAGAGCTGACTAAAATTAAAGCCGAGTC
TTTGCCTTACCATATGGTGCAGTTTGCCATTGCACCGCAAGACAGAACCGTGCTGCACGGTCTTATCGCAAAGCGATTTC
AGCAGATGCTCGCCGAAGGCTTTATCGGTGAAGTGGAAACCCTGAAGGCTCGCGGGGATCTGCACCTTGAACTGCCCTCA
ATGCGCTGCGTAGGTTACAGGCAGGCATGGCAGTATCTGGATGGTGAATTTGACCATGCTACCATGGTCGAAAAAGCTGT
GGCCGCCACTAGGCAATTGGCAAAGCGTCAATTAACATGGTTGCGGGGATGGCCTGAGTTACACTGGTTGGCCAGCGGCG
ACGAAGGGAATTTAGACAAACTTGTGCAGCAATCCCGGTAG

Upstream 100 bases:

>100_bases
CAGCGTATTCAGTCCCTTGAAGCGACTGAGCGGAATAAGATTTTAGAGAGTGCCAGAGATTTGCCCTGGCAGACATGGCT
AGATGAATACAAACACTGAC

Downstream 100 bases:

>100_bases
TGAATCTGCTTTTGCTGTATACTACTCAATTCAAACAGAAAACGTTTGCATTGGACTTTTATAATTTAAAAAGGAAATAA
CAAATGGCTAAGGGGCAATC

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 306; Mature: 305

Protein sequence:

>306_residues
MTSLPKVLFLMGPTASGKTALALDMAEHHNCEIISVDSALIYRGMDIGTAKPTASELARAPHKLIDILDPLESYSAADFR
ADAVREIEETLSRGKTPLLVGGTMMYFKTLLDGLSPLPSADDAVRAQIAAEVEARGWQALHDELRNIDPVSAERIHPNDP
QRLSRAIEVYRISGKTLTELTKIKAESLPYHMVQFAIAPQDRTVLHGLIAKRFQQMLAEGFIGEVETLKARGDLHLELPS
MRCVGYRQAWQYLDGEFDHATMVEKAVAATRQLAKRQLTWLRGWPELHWLASGDEGNLDKLVQQSR

Sequences:

>Translated_306_residues
MTSLPKVLFLMGPTASGKTALALDMAEHHNCEIISVDSALIYRGMDIGTAKPTASELARAPHKLIDILDPLESYSAADFR
ADAVREIEETLSRGKTPLLVGGTMMYFKTLLDGLSPLPSADDAVRAQIAAEVEARGWQALHDELRNIDPVSAERIHPNDP
QRLSRAIEVYRISGKTLTELTKIKAESLPYHMVQFAIAPQDRTVLHGLIAKRFQQMLAEGFIGEVETLKARGDLHLELPS
MRCVGYRQAWQYLDGEFDHATMVEKAVAATRQLAKRQLTWLRGWPELHWLASGDEGNLDKLVQQSR
>Mature_305_residues
TSLPKVLFLMGPTASGKTALALDMAEHHNCEIISVDSALIYRGMDIGTAKPTASELARAPHKLIDILDPLESYSAADFRA
DAVREIEETLSRGKTPLLVGGTMMYFKTLLDGLSPLPSADDAVRAQIAAEVEARGWQALHDELRNIDPVSAERIHPNDPQ
RLSRAIEVYRISGKTLTELTKIKAESLPYHMVQFAIAPQDRTVLHGLIAKRFQQMLAEGFIGEVETLKARGDLHLELPSM
RCVGYRQAWQYLDGEFDHATMVEKAVAATRQLAKRQLTWLRGWPELHWLASGDEGNLDKLVQQSR

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=316, Percent_Identity=27.5316455696203, Blast_Score=110, Evalue=1e-24,
Organism=Escherichia coli, GI1790613, Length=290, Percent_Identity=64.1379310344827, Blast_Score=390, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI25144712, Length=274, Percent_Identity=29.5620437956204, Blast_Score=103, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6324848, Length=288, Percent_Identity=26.7361111111111, Blast_Score=121, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24649830, Length=234, Percent_Identity=35.4700854700855, Blast_Score=122, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_SHEAM (A1SA22)

Other databases:

- EMBL:   CP000507
- RefSeq:   YP_928898.1
- ProteinModelPortal:   A1SA22
- SMR:   A1SA22
- STRING:   A1SA22
- GeneID:   4605273
- GenomeReviews:   CP000507_GR
- KEGG:   saz:Sama_3026
- NMPDR:   fig|326297.7.peg.2843
- eggNOG:   COG0324
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- PhylomeDB:   A1SA22
- ProtClustDB:   PRK00091
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 34061; Mature: 33929

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSLPKVLFLMGPTASGKTALALDMAEHHNCEIISVDSALIYRGMDIGTAKPTASELARA
CCCCCCEEEEECCCCCCCEEEEEEHHHCCCCEEEEECHHHHHCCCCCCCCCCCHHHHHHC
PHKLIDILDPLESYSAADFRADAVREIEETLSRGKTPLLVGGTMMYFKTLLDGLSPLPSA
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHCCCCCCCC
DDAVRAQIAAEVEARGWQALHDELRNIDPVSAERIHPNDPQRLSRAIEVYRISGKTLTEL
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHH
TKIKAESLPYHMVQFAIAPQDRTVLHGLIAKRFQQMLAEGFIGEVETLKARGDLHLELPS
HHHHHHCCCHHHHHEEECCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCEEEECCC
MRCVGYRQAWQYLDGEFDHATMVEKAVAATRQLAKRQLTWLRGWPELHWLASGDEGNLDK
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHH
LVQQSR
HHHCCC
>Mature Secondary Structure 
TSLPKVLFLMGPTASGKTALALDMAEHHNCEIISVDSALIYRGMDIGTAKPTASELARA
CCCCCEEEEECCCCCCCEEEEEEHHHCCCCEEEEECHHHHHCCCCCCCCCCCHHHHHHC
PHKLIDILDPLESYSAADFRADAVREIEETLSRGKTPLLVGGTMMYFKTLLDGLSPLPSA
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHCCCCCCCC
DDAVRAQIAAEVEARGWQALHDELRNIDPVSAERIHPNDPQRLSRAIEVYRISGKTLTEL
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHH
TKIKAESLPYHMVQFAIAPQDRTVLHGLIAKRFQQMLAEGFIGEVETLKARGDLHLELPS
HHHHHHCCCHHHHHEEECCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCEEEECCC
MRCVGYRQAWQYLDGEFDHATMVEKAVAATRQLAKRQLTWLRGWPELHWLASGDEGNLDK
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHH
LVQQSR
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA