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The map label for this gene is pldA [H]

Identifier: 119776025

GI number: 119776025

Start: 3435649

End: 3436533

Strand: Direct

Name: pldA [H]

Synonym: Sama_2893

Alternate gene names: 119776025

Gene position: 3435649-3436533 (Clockwise)

Preceding gene: 119776019

Following gene: 119776026

Centisome position: 79.78

GC content: 49.04

Gene sequence:

>885_bases
ATGAAAAACTTAATGAGATATTTACTACCCCTGTTGTTTATTTCCCATGCTGCCTTGGCAGAAGAACCCTCTCTGGTGGA
TGAAAGGGTGAAAGATGAGCTTGCTACCTCAGAGCAACCCTTCGTGATCACCCCCCATAAGGTGAACTACATATTGCCGG
TGACCTACAACAGTTCCCCCAATATGAAGCCCTTCGAAGAAGAAGCGGCGGAGCATCCCTTCACCCTGGACGAGATGGAA
GCCAAATTCCAGATAAGCTTTAAGTTTCCACTCTGGTATAACGTCTTTGGTGACAACGGCCACCTGTTTTTTGCCTATAC
CAACCAGTCTTACTGGCAGGTATACAACAAAGACATTTCCTCGCCCTTTCGTGAAACCAACCACGAACCTGAGCTCTTTA
TGTTGTTCAATAACGACTGGGAAGTGTTTGGCCTGACCAACTCCTTCTGGGGCTTTGGCGCCGTACACCAGTCAAATGGC
AAGGCAGGTGATTTCTCCCGCAGCTGGAACCGCGTCTACGGCACCATGGTGTTCGACCGGGGCCCCCTGGCCATTGGCGC
CAAGGTATGGTGGCGAATTCCGGAGGATGAAAAAGAATATGAAGGCGATCCCCATGGCGATGACAACCCGGATATCGAAG
ATTACATGGGCAATTTTGAACTGCAGGCTGTGTATGGTCTGGAGGAACATCGTTTTACCATGCTGCTCAGAAACAATCTC
GACAGCCCTAACCGGGGTGCCTTTGAGTTCACCTGGAGCTATCCCATCATAGGCAACCTGCGACTCTATACCCAATACTT
CAATGGTTATGGTGAGAGCCTGATAGACTATAACGCTCACACCCAAAGGGTTGGTATTGGCATATCAATCAACGACTTAC
TTTAA

Upstream 100 bases:

>100_bases
AGCGACTTCACGTTGAGCCATGGGGAATTCTGGTAAATGCGCGGCGGGGGCGTTAATCTGCCCTACAACAAGATAGGCGC
TATATGTTCATGGAAAGACA

Downstream 100 bases:

>100_bases
GAGAACGCTAACCCATGAAGGCCTCTTCGGCCGGACAGCCAACCGCCACGCCACCCACCTTTGATGCTGTGTTGGCGGTG
CTGGTCATTATCATTACCGT

Product: phospholipase A(1)

Products: NA

Alternate protein names: Detergent-resistant phospholipase A; DR-phospholipase A; Outer membrane phospholipase A; OM PLA; OMPLA; Phosphatidylcholine 1-acylhydrolase [H]

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MKNLMRYLLPLLFISHAALAEEPSLVDERVKDELATSEQPFVITPHKVNYILPVTYNSSPNMKPFEEEAAEHPFTLDEME
AKFQISFKFPLWYNVFGDNGHLFFAYTNQSYWQVYNKDISSPFRETNHEPELFMLFNNDWEVFGLTNSFWGFGAVHQSNG
KAGDFSRSWNRVYGTMVFDRGPLAIGAKVWWRIPEDEKEYEGDPHGDDNPDIEDYMGNFELQAVYGLEEHRFTMLLRNNL
DSPNRGAFEFTWSYPIIGNLRLYTQYFNGYGESLIDYNAHTQRVGIGISINDLL

Sequences:

>Translated_294_residues
MKNLMRYLLPLLFISHAALAEEPSLVDERVKDELATSEQPFVITPHKVNYILPVTYNSSPNMKPFEEEAAEHPFTLDEME
AKFQISFKFPLWYNVFGDNGHLFFAYTNQSYWQVYNKDISSPFRETNHEPELFMLFNNDWEVFGLTNSFWGFGAVHQSNG
KAGDFSRSWNRVYGTMVFDRGPLAIGAKVWWRIPEDEKEYEGDPHGDDNPDIEDYMGNFELQAVYGLEEHRFTMLLRNNL
DSPNRGAFEFTWSYPIIGNLRLYTQYFNGYGESLIDYNAHTQRVGIGISINDLL
>Mature_294_residues
MKNLMRYLLPLLFISHAALAEEPSLVDERVKDELATSEQPFVITPHKVNYILPVTYNSSPNMKPFEEEAAEHPFTLDEME
AKFQISFKFPLWYNVFGDNGHLFFAYTNQSYWQVYNKDISSPFRETNHEPELFMLFNNDWEVFGLTNSFWGFGAVHQSNG
KAGDFSRSWNRVYGTMVFDRGPLAIGAKVWWRIPEDEKEYEGDPHGDDNPDIEDYMGNFELQAVYGLEEHRFTMLLRNNL
DSPNRGAFEFTWSYPIIGNLRLYTQYFNGYGESLIDYNAHTQRVGIGISINDLL

Specific function: Hydrolysis of phosphatidylcholine with phospholipase A2 (EC 3.1.1.4) and phospholipase A1 (EC 3.1.1.32) activities [H]

COG id: COG2829

COG function: function code M; Outer membrane phospholipase A

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein. Note=One of the very few enzymes located there (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phospholipase A1 family [H]

Homologues:

Organism=Escherichia coli, GI2367300, Length=302, Percent_Identity=35.4304635761589, Blast_Score=182, Evalue=2e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003187 [H]

Pfam domain/function: PF02253 PLA1 [H]

EC number: =3.1.1.32; =3.1.1.4 [H]

Molecular weight: Translated: 34244; Mature: 34244

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNLMRYLLPLLFISHAALAEEPSLVDERVKDELATSEQPFVITPHKVNYILPVTYNSSP
CHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEECCCCEEEEEEEEECCCC
NMKPFEEEAAEHPFTLDEMEAKFQISFKFPLWYNVFGDNGHLFFAYTNQSYWQVYNKDIS
CCCCCHHHHHCCCCCHHCCCEEEEEEEECCEEEEEECCCCEEEEEECCCCEEHHHHHHHC
SPFRETNHEPELFMLFNNDWEVFGLTNSFWGFGAVHQSNGKAGDFSRSWNRVYGTMVFDR
CHHHHCCCCCCEEEEECCCEEEEECCCCCCCCCEEECCCCCCCCCCCCHHHEEEEEEECC
GPLAIGAKVWWRIPEDEKEYEGDPHGDDNPDIEDYMGNFELQAVYGLEEHRFTMLLRNNL
CCEEECCEEEEECCCCHHHCCCCCCCCCCCCHHHHCCCEEEEEEECCCHHHEEEEEECCC
DSPNRGAFEFTWSYPIIGNLRLYTQYFNGYGESLIDYNAHTQRVGIGISINDLL
CCCCCCEEEEEECCCEECCEEEEEEHHHCCCCHHEECCCCCEEEEEEEEHHCCC
>Mature Secondary Structure
MKNLMRYLLPLLFISHAALAEEPSLVDERVKDELATSEQPFVITPHKVNYILPVTYNSSP
CHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEECCCCEEEEEEEEECCCC
NMKPFEEEAAEHPFTLDEMEAKFQISFKFPLWYNVFGDNGHLFFAYTNQSYWQVYNKDIS
CCCCCHHHHHCCCCCHHCCCEEEEEEEECCEEEEEECCCCEEEEEECCCCEEHHHHHHHC
SPFRETNHEPELFMLFNNDWEVFGLTNSFWGFGAVHQSNGKAGDFSRSWNRVYGTMVFDR
CHHHHCCCCCCEEEEECCCEEEEECCCCCCCCCEEECCCCCCCCCCCCHHHEEEEEEECC
GPLAIGAKVWWRIPEDEKEYEGDPHGDDNPDIEDYMGNFELQAVYGLEEHRFTMLLRNNL
CCEEECCEEEEECCCCHHHCCCCCCCCCCCCHHHHCCCEEEEEEECCCHHHEEEEEECCC
DSPNRGAFEFTWSYPIIGNLRLYTQYFNGYGESLIDYNAHTQRVGIGISINDLL
CCCCCCEEEEEECCCEECCEEEEEEHHHCCCCHHEECCCCCEEEEEEEEHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]