Click here to switch to the map view.

The map label for this gene is fhlA [H]

Identifier: 119775966

GI number: 119775966

Start: 3370684

End: 3372108

Strand: Direct

Name: fhlA [H]

Synonym: Sama_2834

Alternate gene names: 119775966

Gene position: 3370684-3372108 (Clockwise)

Preceding gene: 119775964

Following gene: 119775967

Centisome position: 78.28

GC content: 56.77

Gene sequence:

>1425_bases
ATGCCGACACAGGAAAGCTTTTTACCGGAAAACCGCCTGCTGCTGGATGCCGTCAGCGAAGGTATCTATGGTTTTGATTT
GGATGGCCATGCGGTTTTTATCAATCCAGCGGCCGAGCGTATGACCGGCTGGCAGGCAGATGAACTGCTCGGCAAAACCA
TCCATAACTTCCACCACCACAGCCATGCCGATGGCAGCCACTATCCCCACGAAGACTGCCCGATCTATCAAACACTTAAG
GATGGCAAGGCACGGGAGATCAGCGACGAAGTATTCTGGCGCAAGGATGGCAGCAGCTTCCCGGTACATTACAGCTCAAC
TCCGGTATGGCAGGACGGCAAGCTCAAAGGCGTGGTCGCGATATTTCGCGACATCAGTTTACAAAAGCAAACCGAGCAGT
CGCTGCGCCAGGCGCTGGAGCAGGTGCAGGCGCTGTCGGAGCAGTTGGCGTCTGAAAACTCACTGCTGCAGGCCGAACTT
GAGGATAAGCGCGGCCCCTCGCAGATAACCGGCAGCAGCGACTGTATTCAAAATCTGCAGAAGCAAATCCGCCTGGTGGC
CAACACCGACAGCACAGTTTTGATACAGGGCGAAAACGGCACCGGCAAGGAGCTGGTCGCCCGCGAGCTTCATCAACTCA
GCAGTCGCCGTGATAAGCCGATGATCTCGGTTAACTGCGCCGCCTTCTCGGCATCTCTGTTGGAAAGTGAACTTTTTGGT
CATGAAAAGGGCGCTTTCACCGGCGCCAGCAATCGCCGCAAGGGCCGCTTTGAACTGGCCCACAAGGGTACCCTGTTTCT
GGATGAAGTGGCCGAGCTCAGCCTTGAGGCGCAGTCCAAGCTGCTTAGGGTTATTCAGGAGCAGGAGTTTGAACGTGTGG
GCGGCAGCGAGCGCATCAAGGTGGATATCCGCCTGATAGCCGCCACCCATCACGACTTGCAAAAAAGAGTCGAACAGGGA
CTGTTCAGGATGGATCTCTTCTACCGCCTGAACGTGTTTCCACTTAAGGTACCGCCGCTTCGTCAGCGCGCCGGAGATAT
TCCCGAACTGGTGCAGCAACTGCTCAGGAGCCTGTCACAAAAGCTCGGCAAGCCGCTGGCAGGGGTCGATGCCGTCAGCC
TCAAACGCTTGCAGCAATACAGTTGGCCGGGCAATGTGCGCGAACTGCAAAACCTGCTGGAGCGGGAAGCGATTCTCACC
CATCAGCCGGTTCTCAAAATGAGCTCAATCCCCGGCAGCCAAATGCCGCCTGTTACAGGACAAACCCTGGCCGAGGTAGA
GGCAAGTCATATTCTCTTAACCCTGGAAAATTGCGACTGGCGCATCTCGGGCCCCGGCGGTGTGGCGGCGGTATTGGGGC
TACCACCCAGCACCCTGAGATCCAGGATGCAAAAACTCGGCATTCGCCGATCTGCCAGGCAGTAA

Upstream 100 bases:

>100_bases
TCGTGACTTGCTCAGCAAAGTGCCCTGATGCGCCCATGACGCCCCAACAGGGGCAGGCTATACTGCCGCCATTAATCACT
AACCAGCCCCGACCAACACC

Downstream 100 bases:

>100_bases
AATATCACCAAGCCATGAAGCCCACCCCGGCTTCACCCCCTCTGTTATGAAATCCGTTCCAGCCTGATGGAACTTGGTCA
CGAAATATCGCCAAATCACG

Product: sigma-54 dependent transcriptional regulator/sensory box protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 474; Mature: 473

Protein sequence:

>474_residues
MPTQESFLPENRLLLDAVSEGIYGFDLDGHAVFINPAAERMTGWQADELLGKTIHNFHHHSHADGSHYPHEDCPIYQTLK
DGKAREISDEVFWRKDGSSFPVHYSSTPVWQDGKLKGVVAIFRDISLQKQTEQSLRQALEQVQALSEQLASENSLLQAEL
EDKRGPSQITGSSDCIQNLQKQIRLVANTDSTVLIQGENGTGKELVARELHQLSSRRDKPMISVNCAAFSASLLESELFG
HEKGAFTGASNRRKGRFELAHKGTLFLDEVAELSLEAQSKLLRVIQEQEFERVGGSERIKVDIRLIAATHHDLQKRVEQG
LFRMDLFYRLNVFPLKVPPLRQRAGDIPELVQQLLRSLSQKLGKPLAGVDAVSLKRLQQYSWPGNVRELQNLLEREAILT
HQPVLKMSSIPGSQMPPVTGQTLAEVEASHILLTLENCDWRISGPGGVAAVLGLPPSTLRSRMQKLGIRRSARQ

Sequences:

>Translated_474_residues
MPTQESFLPENRLLLDAVSEGIYGFDLDGHAVFINPAAERMTGWQADELLGKTIHNFHHHSHADGSHYPHEDCPIYQTLK
DGKAREISDEVFWRKDGSSFPVHYSSTPVWQDGKLKGVVAIFRDISLQKQTEQSLRQALEQVQALSEQLASENSLLQAEL
EDKRGPSQITGSSDCIQNLQKQIRLVANTDSTVLIQGENGTGKELVARELHQLSSRRDKPMISVNCAAFSASLLESELFG
HEKGAFTGASNRRKGRFELAHKGTLFLDEVAELSLEAQSKLLRVIQEQEFERVGGSERIKVDIRLIAATHHDLQKRVEQG
LFRMDLFYRLNVFPLKVPPLRQRAGDIPELVQQLLRSLSQKLGKPLAGVDAVSLKRLQQYSWPGNVRELQNLLEREAILT
HQPVLKMSSIPGSQMPPVTGQTLAEVEASHILLTLENCDWRISGPGGVAAVLGLPPSTLRSRMQKLGIRRSARQ
>Mature_473_residues
PTQESFLPENRLLLDAVSEGIYGFDLDGHAVFINPAAERMTGWQADELLGKTIHNFHHHSHADGSHYPHEDCPIYQTLKD
GKAREISDEVFWRKDGSSFPVHYSSTPVWQDGKLKGVVAIFRDISLQKQTEQSLRQALEQVQALSEQLASENSLLQAELE
DKRGPSQITGSSDCIQNLQKQIRLVANTDSTVLIQGENGTGKELVARELHQLSSRRDKPMISVNCAAFSASLLESELFGH
EKGAFTGASNRRKGRFELAHKGTLFLDEVAELSLEAQSKLLRVIQEQEFERVGGSERIKVDIRLIAATHHDLQKRVEQGL
FRMDLFYRLNVFPLKVPPLRQRAGDIPELVQQLLRSLSQKLGKPLAGVDAVSLKRLQQYSWPGNVRELQNLLEREAILTH
QPVLKMSSIPGSQMPPVTGQTLAEVEASHILLTLENCDWRISGPGGVAAVLGLPPSTLRSRMQKLGIRRSARQ

Specific function: Required for induction of expression of the formate dehydrogenase H and hydrogenase-3 structural genes [H]

COG id: COG3604

COG function: function code KT; Transcriptional regulator containing GAF, AAA-type ATPase, and DNA binding domains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1789087, Length=342, Percent_Identity=47.953216374269, Blast_Score=312, Evalue=4e-86,
Organism=Escherichia coli, GI87082117, Length=350, Percent_Identity=47.1428571428571, Blast_Score=302, Evalue=4e-83,
Organism=Escherichia coli, GI1790437, Length=275, Percent_Identity=49.0909090909091, Blast_Score=254, Evalue=9e-69,
Organism=Escherichia coli, GI1788550, Length=253, Percent_Identity=49.0118577075099, Blast_Score=249, Evalue=2e-67,
Organism=Escherichia coli, GI87082152, Length=255, Percent_Identity=47.843137254902, Blast_Score=227, Evalue=1e-60,
Organism=Escherichia coli, GI1788905, Length=244, Percent_Identity=46.3114754098361, Blast_Score=225, Evalue=6e-60,
Organism=Escherichia coli, GI1790299, Length=271, Percent_Identity=43.9114391143911, Blast_Score=223, Evalue=2e-59,
Organism=Escherichia coli, GI1789233, Length=245, Percent_Identity=46.530612244898, Blast_Score=223, Evalue=2e-59,
Organism=Escherichia coli, GI1786524, Length=314, Percent_Identity=40.7643312101911, Blast_Score=211, Evalue=5e-56,
Organism=Escherichia coli, GI87081872, Length=228, Percent_Identity=47.8070175438597, Blast_Score=206, Evalue=2e-54,
Organism=Escherichia coli, GI1787583, Length=235, Percent_Identity=39.5744680851064, Blast_Score=172, Evalue=4e-44,
Organism=Escherichia coli, GI87081858, Length=296, Percent_Identity=35.1351351351351, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI1789828, Length=228, Percent_Identity=36.4035087719298, Blast_Score=130, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 52952; Mature: 52821

Theoretical pI: Translated: 7.28; Mature: 7.28

Prosite motif: PS50112 PAS ; PS50113 PAC ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTQESFLPENRLLLDAVSEGIYGFDLDGHAVFINPAAERMTGWQADELLGKTIHNFHHH
CCCCCCCCCCCHHHHHHHHCCCEEECCCCCEEEECCHHHHHCCCCHHHHHHHHHHHHHCC
SHADGSHYPHEDCPIYQTLKDGKAREISDEVFWRKDGSSFPVHYSSTPVWQDGKLKGVVA
CCCCCCCCCCCCCCHHHHHCCCCCHHCCHHHHEECCCCCCCEECCCCCCCCCCCHHHHHH
IFRDISLQKQTEQSLRQALEQVQALSEQLASENSLLQAELEDKRGPSQITGSSDCIQNLQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHCCCCCCCCCCCHHHHHHHH
KQIRLVANTDSTVLIQGENGTGKELVARELHQLSSRRDKPMISVNCAAFSASLLESELFG
HHHEEEECCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHC
HEKGAFTGASNRRKGRFELAHKGTLFLDEVAELSLEAQSKLLRVIQEQEFERVGGSERIK
CCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
VDIRLIAATHHDLQKRVEQGLFRMDLFYRLNVFPLKVPPLRQRAGDIPELVQQLLRSLSQ
EEEEEEEHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCHHHHCCCHHHHHHHHHHHHHH
KLGKPLAGVDAVSLKRLQQYSWPGNVRELQNLLEREAILTHQPVLKMSSIPGSQMPPVTG
HHCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCC
QTLAEVEASHILLTLENCDWRISGPGGVAAVLGLPPSTLRSRMQKLGIRRSARQ
HHHHHHCCCEEEEEEECCCEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PTQESFLPENRLLLDAVSEGIYGFDLDGHAVFINPAAERMTGWQADELLGKTIHNFHHH
CCCCCCCCCCHHHHHHHHCCCEEECCCCCEEEECCHHHHHCCCCHHHHHHHHHHHHHCC
SHADGSHYPHEDCPIYQTLKDGKAREISDEVFWRKDGSSFPVHYSSTPVWQDGKLKGVVA
CCCCCCCCCCCCCCHHHHHCCCCCHHCCHHHHEECCCCCCCEECCCCCCCCCCCHHHHHH
IFRDISLQKQTEQSLRQALEQVQALSEQLASENSLLQAELEDKRGPSQITGSSDCIQNLQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHCCCCCCCCCCCHHHHHHHH
KQIRLVANTDSTVLIQGENGTGKELVARELHQLSSRRDKPMISVNCAAFSASLLESELFG
HHHEEEECCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHC
HEKGAFTGASNRRKGRFELAHKGTLFLDEVAELSLEAQSKLLRVIQEQEFERVGGSERIK
CCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
VDIRLIAATHHDLQKRVEQGLFRMDLFYRLNVFPLKVPPLRQRAGDIPELVQQLLRSLSQ
EEEEEEEHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCHHHHCCCHHHHHHHHHHHHHH
KLGKPLAGVDAVSLKRLQQYSWPGNVRELQNLLEREAILTHQPVLKMSSIPGSQMPPVTG
HHCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCC
QTLAEVEASHILLTLENCDWRISGPGGVAAVLGLPPSTLRSRMQKLGIRRSARQ
HHHHHHCCCEEEEEEECCCEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2280686; 2118503; 9278503 [H]