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The map label for this gene is gatA [H]

Identifier: 119775654

GI number: 119775654

Start: 3030832

End: 3032535

Strand: Direct

Name: gatA [H]

Synonym: Sama_2522

Alternate gene names: 119775654

Gene position: 3030832-3032535 (Clockwise)

Preceding gene: 119775653

Following gene: 119775655

Centisome position: 70.38

GC content: 61.09

Gene sequence:

>1704_bases
ATGTTCGAAGTAACCGAAGTATCCATTGCCGAGCTGCGCGGCGCGCTCGAATCCGGCCGCACCACGGCGGTCGAACTGGT
TAAAGCGTATCTTGCCCGTATCGAAGCCTATGATGGCGCCGATACCGACACCAAACTCAATGCGGTGGTGGTACACAACC
CCGATGCACTGAAAGAAGCCGAGGCCTCCGATGCGCGCCGCGCCCGCGGCGAAACGTTGGGGCCACTCGATGGCATCCCC
TACACCGCCAAAGACAGCTATCTGGTGAAGGGCCTGACCGCCGCTTCCGGCAGCCCGGCCTTTAAAGACTTGGTTGCCCA
GCGTGATGCCTTCACCGTGGAGCGCCTGCGCGCCAATGGCGCAATCTGCCTTGGCAAAACCAATATGCCGCCCATGGCCA
ATGGCGGCATGCAGCGTGGTGTGTATGGCCGCGCCGAAAGCCCCTATAACGCAGCTTATCTCACTGCGCCCTTCGCCTCC
GGCTCATCCAATGGCGCCGGTACTGCCACCGCCGCCAGCTTTTGCGCCTTTGGTCTGGCCGAAGAAACCTGGTCCAGCGG
CCGTGGCCCGGCGTCCAACAATGGTTTGTGTGCTTACACCCCGTCCCGCGGCGTGATTTCGGTACGCGGCAACTGGCCTC
TGACCCCCACCATGGACGTGGTAGTGCCCTACGCCCGCACCATGGCTGACCTGTTGGAAGTCTTGGACGTAGTAGTCGCC
GACGATAGTGAAACCCGCGGCGACCTGTGGCGTATGCAGCCCTGGGTTCAAATCCCCAAGGCTTCCGAAGTGCGCCCCGA
GTCGTATATCGCCCTCGCCTGTGGCGCAGATAGCCTTAAAGGCAAACGCTTTGGTGTGCCCAAGATGTTTATCAATAAAG
ATGAACTCGCCGGCACCAGTGAAAACCCAGGTATTGGCGGCCCGACCGGTCAGCGCATTCATACCCGTGACTCTGTGATT
GCCCTGTGGGAGCAGGCGCGAGCGGCGCTGGAAGCCGCTGGCGCCGAGGTGATTGAAGTGGACTTCCCGCTGGTGTCCAA
CTGCGAAGGCGACAGACCCGGCGCACCAACCGTATTCAACCGCGGCATAGTCACTCCTGAGTTTTTGCACGACGAGCTGT
GGGAGCTCTCCGGCTGGGCCTTCGATGACTTCCTGCGTGCCAATGGTGACCCCAAGCTCAATAAGCTGGAGGACGTAGAT
GGGCCGAAGATTTTCCCCCACGACCCGGGCACACTGCCGAACCGCGAGGGCGATCTGGCCGCCGGTATGGATGAGTACGT
CAACATGGCCAAGCGCGGCATCACGCCGTGGAATGAAATTGCCACAGTCCCCGATGGCCTGCGCGGTCTGGAAAAGACCC
GCAAGCTTGACCTCGAAGACTGGATGGATGCGCTTAAGCTGGATGCTGTGCTCTTCCCCACCGTGGCCGACGTGGGCCCT
GCCGATGCCGACGTGAACCCAGCTTCTGCCGATATCGCCTGGAGCAACGGCGTTTGGGTTGCCAATGGCAACCTCGCCAT
CCGTCACCTGGGCGTACCCACTGTGACTGTGCCCATGGGCATCATGGCCGATATCGGCATGCCTGTGGGTCTGACGTTTG
CTGGCCGTGCCTACGATGACAACGCGCTCTTAAGCCTGGCTTCGGCCTTTGAGTCCACCGGTAATAAGCGCCACATACCA
CCACGCACACCTAAGCTATCCTGA

Upstream 100 bases:

>100_bases
GTGAGATCCTGCTTGGCGGTGGCAACATCCACTGCATTACCCAGCAAATCCCATCCGGCAAATAATCGGCCTGTTCATTC
ACCTTTTCGGAGTTACCCGT

Downstream 100 bases:

>100_bases
CGACTTCAATTAACTCGCTGATAGTGAATTGAAAGCCACCCTCTGGGTGGCTTTTTGCTGTATCCGATTCAAGGAACATG
ACCGACTGGGGCTTCTTTGC

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 567; Mature: 567

Protein sequence:

>567_residues
MFEVTEVSIAELRGALESGRTTAVELVKAYLARIEAYDGADTDTKLNAVVVHNPDALKEAEASDARRARGETLGPLDGIP
YTAKDSYLVKGLTAASGSPAFKDLVAQRDAFTVERLRANGAICLGKTNMPPMANGGMQRGVYGRAESPYNAAYLTAPFAS
GSSNGAGTATAASFCAFGLAEETWSSGRGPASNNGLCAYTPSRGVISVRGNWPLTPTMDVVVPYARTMADLLEVLDVVVA
DDSETRGDLWRMQPWVQIPKASEVRPESYIALACGADSLKGKRFGVPKMFINKDELAGTSENPGIGGPTGQRIHTRDSVI
ALWEQARAALEAAGAEVIEVDFPLVSNCEGDRPGAPTVFNRGIVTPEFLHDELWELSGWAFDDFLRANGDPKLNKLEDVD
GPKIFPHDPGTLPNREGDLAAGMDEYVNMAKRGITPWNEIATVPDGLRGLEKTRKLDLEDWMDALKLDAVLFPTVADVGP
ADADVNPASADIAWSNGVWVANGNLAIRHLGVPTVTVPMGIMADIGMPVGLTFAGRAYDDNALLSLASAFESTGNKRHIP
PRTPKLS

Sequences:

>Translated_567_residues
MFEVTEVSIAELRGALESGRTTAVELVKAYLARIEAYDGADTDTKLNAVVVHNPDALKEAEASDARRARGETLGPLDGIP
YTAKDSYLVKGLTAASGSPAFKDLVAQRDAFTVERLRANGAICLGKTNMPPMANGGMQRGVYGRAESPYNAAYLTAPFAS
GSSNGAGTATAASFCAFGLAEETWSSGRGPASNNGLCAYTPSRGVISVRGNWPLTPTMDVVVPYARTMADLLEVLDVVVA
DDSETRGDLWRMQPWVQIPKASEVRPESYIALACGADSLKGKRFGVPKMFINKDELAGTSENPGIGGPTGQRIHTRDSVI
ALWEQARAALEAAGAEVIEVDFPLVSNCEGDRPGAPTVFNRGIVTPEFLHDELWELSGWAFDDFLRANGDPKLNKLEDVD
GPKIFPHDPGTLPNREGDLAAGMDEYVNMAKRGITPWNEIATVPDGLRGLEKTRKLDLEDWMDALKLDAVLFPTVADVGP
ADADVNPASADIAWSNGVWVANGNLAIRHLGVPTVTVPMGIMADIGMPVGLTFAGRAYDDNALLSLASAFESTGNKRHIP
PRTPKLS
>Mature_567_residues
MFEVTEVSIAELRGALESGRTTAVELVKAYLARIEAYDGADTDTKLNAVVVHNPDALKEAEASDARRARGETLGPLDGIP
YTAKDSYLVKGLTAASGSPAFKDLVAQRDAFTVERLRANGAICLGKTNMPPMANGGMQRGVYGRAESPYNAAYLTAPFAS
GSSNGAGTATAASFCAFGLAEETWSSGRGPASNNGLCAYTPSRGVISVRGNWPLTPTMDVVVPYARTMADLLEVLDVVVA
DDSETRGDLWRMQPWVQIPKASEVRPESYIALACGADSLKGKRFGVPKMFINKDELAGTSENPGIGGPTGQRIHTRDSVI
ALWEQARAALEAAGAEVIEVDFPLVSNCEGDRPGAPTVFNRGIVTPEFLHDELWELSGWAFDDFLRANGDPKLNKLEDVD
GPKIFPHDPGTLPNREGDLAAGMDEYVNMAKRGITPWNEIATVPDGLRGLEKTRKLDLEDWMDALKLDAVLFPTVADVGP
ADADVNPASADIAWSNGVWVANGNLAIRHLGVPTVTVPMGIMADIGMPVGLTFAGRAYDDNALLSLASAFESTGNKRHIP
PRTPKLS

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=572, Percent_Identity=25.5244755244755, Blast_Score=120, Evalue=4e-27,
Organism=Homo sapiens, GI195972892, Length=245, Percent_Identity=28.9795918367347, Blast_Score=96, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17537465, Length=256, Percent_Identity=27.34375, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17543272, Length=236, Percent_Identity=25.8474576271186, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6319685, Length=488, Percent_Identity=26.4344262295082, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24644968, Length=254, Percent_Identity=30.7086614173228, Blast_Score=96, Evalue=1e-19,
Organism=Drosophila melanogaster, GI161078093, Length=270, Percent_Identity=31.4814814814815, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24648113, Length=529, Percent_Identity=23.6294896030246, Blast_Score=88, Evalue=2e-17,
Organism=Drosophila melanogaster, GI21356731, Length=244, Percent_Identity=29.9180327868852, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI45550774, Length=222, Percent_Identity=28.8288288288288, Blast_Score=74, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24648435, Length=222, Percent_Identity=28.8288288288288, Blast_Score=74, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24648437, Length=222, Percent_Identity=28.8288288288288, Blast_Score=74, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24652985, Length=255, Percent_Identity=29.0196078431373, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI19922090, Length=255, Percent_Identity=29.0196078431373, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24652981, Length=255, Percent_Identity=29.0196078431373, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24652983, Length=255, Percent_Identity=29.0196078431373, Blast_Score=73, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: NA

Molecular weight: Translated: 60470; Mature: 60470

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFEVTEVSIAELRGALESGRTTAVELVKAYLARIEAYDGADTDTKLNAVVVHNPDALKEA
CCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCHHHHHH
EASDARRARGETLGPLDGIPYTAKDSYLVKGLTAASGSPAFKDLVAQRDAFTVERLRANG
HHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHCCHHHHHHHHCCC
AICLGKTNMPPMANGGMQRGVYGRAESPYNAAYLTAPFASGSSNGAGTATAASFCAFGLA
EEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHCH
EETWSSGRGPASNNGLCAYTPSRGVISVRGNWPLTPTMDVVVPYARTMADLLEVLDVVVA
HHHHCCCCCCCCCCCEEEECCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
DDSETRGDLWRMQPWVQIPKASEVRPESYIALACGADSLKGKRFGVPKMFINKDELAGTS
CCCCCCCCCEECCCCEECCCCCCCCCCCEEEEEECCCCCCCCCCCCCHHEECHHHHCCCC
ENPGIGGPTGQRIHTRDSVIALWEQARAALEAAGAEVIEVDFPLVSNCEGDRPGAPTVFN
CCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHCCCEEEEECCCHHCCCCCCCCCCCHHHC
RGIVTPEFLHDELWELSGWAFDDFLRANGDPKLNKLEDVDGPKIFPHDPGTLPNREGDLA
CCCCCHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCHH
AGMDEYVNMAKRGITPWNEIATVPDGLRGLEKTRKLDLEDWMDALKLDAVLFPTVADVGP
CCHHHHHHHHHHCCCCHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCHHHCCCC
ADADVNPASADIAWSNGVWVANGNLAIRHLGVPTVTVPMGIMADIGMPVGLTFAGRAYDD
CCCCCCCCCCCEEECCCEEEECCCEEEEEECCCEEEECHHHHHHCCCCCCEEECCCCCCC
NALLSLASAFESTGNKRHIPPRTPKLS
HHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MFEVTEVSIAELRGALESGRTTAVELVKAYLARIEAYDGADTDTKLNAVVVHNPDALKEA
CCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCHHHHHH
EASDARRARGETLGPLDGIPYTAKDSYLVKGLTAASGSPAFKDLVAQRDAFTVERLRANG
HHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHCCHHHHHHHHCCC
AICLGKTNMPPMANGGMQRGVYGRAESPYNAAYLTAPFASGSSNGAGTATAASFCAFGLA
EEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHCH
EETWSSGRGPASNNGLCAYTPSRGVISVRGNWPLTPTMDVVVPYARTMADLLEVLDVVVA
HHHHCCCCCCCCCCCEEEECCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
DDSETRGDLWRMQPWVQIPKASEVRPESYIALACGADSLKGKRFGVPKMFINKDELAGTS
CCCCCCCCCEECCCCEECCCCCCCCCCCEEEEEECCCCCCCCCCCCCHHEECHHHHCCCC
ENPGIGGPTGQRIHTRDSVIALWEQARAALEAAGAEVIEVDFPLVSNCEGDRPGAPTVFN
CCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHCCCEEEEECCCHHCCCCCCCCCCCHHHC
RGIVTPEFLHDELWELSGWAFDDFLRANGDPKLNKLEDVDGPKIFPHDPGTLPNREGDLA
CCCCCHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCHH
AGMDEYVNMAKRGITPWNEIATVPDGLRGLEKTRKLDLEDWMDALKLDAVLFPTVADVGP
CCHHHHHHHHHHCCCCHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCHHHCCCC
ADADVNPASADIAWSNGVWVANGNLAIRHLGVPTVTVPMGIMADIGMPVGLTFAGRAYDD
CCCCCCCCCCCEEECCCEEEECCCEEEEEECCCEEEECHHHHHHCCCCCCEEECCCCCCC
NALLSLASAFESTGNKRHIPPRTPKLS
HHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA