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The map label for this gene is carA [H]

Identifier: 119775636

GI number: 119775636

Start: 3009178

End: 3010323

Strand: Reverse

Name: carA [H]

Synonym: Sama_2504

Alternate gene names: 119775636

Gene position: 3010323-3009178 (Counterclockwise)

Preceding gene: 119775637

Following gene: 119775635

Centisome position: 69.91

GC content: 52.88

Gene sequence:

>1146_bases
TTGACAAAGTCTGCCTTACTCGTACTCGAAGATGGAACCCTGTTCTCAGGCACTGCAATCGGTGCTGATGGAATTGCTGT
CGGAGAAGTCGTTTTTAACACTTCCATGACAGGCTATCAAGAGATATTGACGGATCCTTCTTATTCCCGCCAGATAGTCA
CACTGACTTATCCGCACATCGGTAATACAGGTACCAACGATGAAGACACTGAATCCAGTGCTGTTCATGCCTGTGGTCTC
ATCATTCGCGATCTTCCTTTAATCGCCAGCAGTTTCCGTTCCACCCAATCCTTAAGTGATTACCTCAAAGCCAACAACGT
GGTAGGTATCGCCGATATCGACACCCGTAAGCTGACCCGTATTCTGCGTGAAAAGGGCGCTCAGGCCGGTTGTATTCTGG
TGGGTGACCTGGACAGCGATAAGGCACTGGCTGCCGCCAAAGCCTTCCCTGGTTTGAAAGGCATGGATTTGGCGAAAGAA
GTGACCACCGCCGAGCGCTACCAGTGGCGCAGTGGCAGCTGGCGTCTGGAAGGCGGTCTGCCTTCAGATACCCCAGAATC
TGAACTCAAGTTCAAAGTGGTTGCCTACGATTACGGCGTTAAAACCAACATTCTGCGTATGCTGGTAGACCGTGGTTGTG
ACGTGACCGTGGTGCCTGCCAAAACTCCAGCCAGCGAAGTGCTGGCCATGAATCCTGACGGTGTGTTTCTGTCCAACGGC
CCTGGTGACCCAGAGCCATGTGACTATGCCATTGCCGCGATTCAGGAAATTCTCACCACAGACATCCCGGTATTCGGTAT
CTGTTTGGGCCATCAGCTGCTCGGTCTCGCCAGCGGTGCCAAGACCCTGAAAATGAAGTTTGGCCACCATGGTGCCAACC
ACCCAGTGGGTGATGTTGAAAAGGGTACTGTGATGATCACCAGCCAGAACCACGGTTTTGCGGTTGATGAGGCTACACTG
CCTGCCAACCTCAAGGTGACTCACAAGTCACTGTTCGATGGTTCTTTGCAGGGCATTCACCGCACCGACAAGCCTGCGTT
CAGCTTCCAGGGTCACCCTGAAGCCAGCCCAGGTCCACACGACTGTGCCCCGCTGTTCGACCATTTCATCGAACTGATTG
AAGAGTATCGCAAGAACGCCAAGTAA

Upstream 100 bases:

>100_bases
TCATCGGAATTTGTCAAAATTTCGTGTTTTAACGGAAACTTGGTAATTTAATCTAAAAAAATCCACACATAACAATAACT
TGGCTCTTTCGGAGGTCGCG

Downstream 100 bases:

>100_bases
GCCGAGCTGGAGAAGATAGAAACAATGCCAAAACGTACCGACATAAAAAGTATTCTGATCCTGGGTGCTGGCCCCATCGT
TATCGGCCAGGCCTGTGAAT

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain [H]

Number of amino acids: Translated: 381; Mature: 380

Protein sequence:

>381_residues
MTKSALLVLEDGTLFSGTAIGADGIAVGEVVFNTSMTGYQEILTDPSYSRQIVTLTYPHIGNTGTNDEDTESSAVHACGL
IIRDLPLIASSFRSTQSLSDYLKANNVVGIADIDTRKLTRILREKGAQAGCILVGDLDSDKALAAAKAFPGLKGMDLAKE
VTTAERYQWRSGSWRLEGGLPSDTPESELKFKVVAYDYGVKTNILRMLVDRGCDVTVVPAKTPASEVLAMNPDGVFLSNG
PGDPEPCDYAIAAIQEILTTDIPVFGICLGHQLLGLASGAKTLKMKFGHHGANHPVGDVEKGTVMITSQNHGFAVDEATL
PANLKVTHKSLFDGSLQGIHRTDKPAFSFQGHPEASPGPHDCAPLFDHFIELIEEYRKNAK

Sequences:

>Translated_381_residues
MTKSALLVLEDGTLFSGTAIGADGIAVGEVVFNTSMTGYQEILTDPSYSRQIVTLTYPHIGNTGTNDEDTESSAVHACGL
IIRDLPLIASSFRSTQSLSDYLKANNVVGIADIDTRKLTRILREKGAQAGCILVGDLDSDKALAAAKAFPGLKGMDLAKE
VTTAERYQWRSGSWRLEGGLPSDTPESELKFKVVAYDYGVKTNILRMLVDRGCDVTVVPAKTPASEVLAMNPDGVFLSNG
PGDPEPCDYAIAAIQEILTTDIPVFGICLGHQLLGLASGAKTLKMKFGHHGANHPVGDVEKGTVMITSQNHGFAVDEATL
PANLKVTHKSLFDGSLQGIHRTDKPAFSFQGHPEASPGPHDCAPLFDHFIELIEEYRKNAK
>Mature_380_residues
TKSALLVLEDGTLFSGTAIGADGIAVGEVVFNTSMTGYQEILTDPSYSRQIVTLTYPHIGNTGTNDEDTESSAVHACGLI
IRDLPLIASSFRSTQSLSDYLKANNVVGIADIDTRKLTRILREKGAQAGCILVGDLDSDKALAAAKAFPGLKGMDLAKEV
TTAERYQWRSGSWRLEGGLPSDTPESELKFKVVAYDYGVKTNILRMLVDRGCDVTVVPAKTPASEVLAMNPDGVFLSNGP
GDPEPCDYAIAAIQEILTTDIPVFGICLGHQLLGLASGAKTLKMKFGHHGANHPVGDVEKGTVMITSQNHGFAVDEATLP
ANLKVTHKSLFDGSLQGIHRTDKPAFSFQGHPEASPGPHDCAPLFDHFIELIEEYRKNAK

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=382, Percent_Identity=39.0052356020942, Blast_Score=244, Evalue=1e-64,
Organism=Homo sapiens, GI169790915, Length=385, Percent_Identity=36.3636363636364, Blast_Score=222, Evalue=4e-58,
Organism=Homo sapiens, GI21361331, Length=385, Percent_Identity=36.3636363636364, Blast_Score=222, Evalue=5e-58,
Organism=Escherichia coli, GI1786215, Length=382, Percent_Identity=80.1047120418848, Blast_Score=635, Evalue=0.0,
Organism=Caenorhabditis elegans, GI193204318, Length=386, Percent_Identity=37.5647668393782, Blast_Score=235, Evalue=2e-62,
Organism=Saccharomyces cerevisiae, GI6324878, Length=392, Percent_Identity=39.2857142857143, Blast_Score=249, Evalue=4e-67,
Organism=Saccharomyces cerevisiae, GI6322331, Length=402, Percent_Identity=35.3233830845771, Blast_Score=232, Evalue=6e-62,
Organism=Drosophila melanogaster, GI45555749, Length=389, Percent_Identity=38.8174807197943, Blast_Score=232, Evalue=3e-61,
Organism=Drosophila melanogaster, GI24642586, Length=389, Percent_Identity=38.8174807197943, Blast_Score=231, Evalue=5e-61,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 40812; Mature: 40680

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKSALLVLEDGTLFSGTAIGADGIAVGEVVFNTSMTGYQEILTDPSYSRQIVTLTYPHI
CCCCEEEEEECCCEEECCEECCCCEEEEHEEECCCCCHHHHHHCCCCCCCEEEEEECCCC
GNTGTNDEDTESSAVHACGLIIRDLPLIASSFRSTQSLSDYLKANNVVGIADIDTRKLTR
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHH
ILREKGAQAGCILVGDLDSDKALAAAKAFPGLKGMDLAKEVTTAERYQWRSGSWRLEGGL
HHHHCCCCCCEEEEECCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHEECCCCEEECCCC
PSDTPESELKFKVVAYDYGVKTNILRMLVDRGCDVTVVPAKTPASEVLAMNPDGVFLSNG
CCCCCCCCEEEEEEEEECCCHHHHHHHHHHCCCCEEEEECCCCHHHEEEECCCCEEECCC
PGDPEPCDYAIAAIQEILTTDIPVFGICLGHQLLGLASGAKTLKMKFGHHGANHPVGDVE
CCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCC
KGTVMITSQNHGFAVDEATLPANLKVTHKSLFDGSLQGIHRTDKPAFSFQGHPEASPGPH
CCEEEEEECCCCEEEECCCCCCCEEEEHHHHHCCHHHCCCCCCCCCCCCCCCCCCCCCCC
DCAPLFDHFIELIEEYRKNAK
CHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TKSALLVLEDGTLFSGTAIGADGIAVGEVVFNTSMTGYQEILTDPSYSRQIVTLTYPHI
CCCEEEEEECCCEEECCEECCCCEEEEHEEECCCCCHHHHHHCCCCCCCEEEEEECCCC
GNTGTNDEDTESSAVHACGLIIRDLPLIASSFRSTQSLSDYLKANNVVGIADIDTRKLTR
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHH
ILREKGAQAGCILVGDLDSDKALAAAKAFPGLKGMDLAKEVTTAERYQWRSGSWRLEGGL
HHHHCCCCCCEEEEECCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHEECCCCEEECCCC
PSDTPESELKFKVVAYDYGVKTNILRMLVDRGCDVTVVPAKTPASEVLAMNPDGVFLSNG
CCCCCCCCEEEEEEEEECCCHHHHHHHHHHCCCCEEEEECCCCHHHEEEECCCCEEECCC
PGDPEPCDYAIAAIQEILTTDIPVFGICLGHQLLGLASGAKTLKMKFGHHGANHPVGDVE
CCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCC
KGTVMITSQNHGFAVDEATLPANLKVTHKSLFDGSLQGIHRTDKPAFSFQGHPEASPGPH
CCEEEEEECCCCEEEECCCCCCCEEEEHHHHHCCHHHCCCCCCCCCCCCCCCCCCCCCCC
DCAPLFDHFIELIEEYRKNAK
CHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]