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The map label for this gene is pilT [H]

Identifier: 119775608

GI number: 119775608

Start: 2978070

End: 2979182

Strand: Reverse

Name: pilT [H]

Synonym: Sama_2475

Alternate gene names: 119775608

Gene position: 2979182-2978070 (Counterclockwise)

Preceding gene: 119775609

Following gene: 119775607

Centisome position: 69.18

GC content: 54.45

Gene sequence:

>1113_bases
ATGGATGTACGTCCCTTTCTGAAGGTGATGGTCGAGCGCAAAGCCTCGGATTTGTTCATCACCGCGGGTTTTCCTCCCAG
CGCCAAAATCGACGGTGAGCTGCGGCCACTGGCGGAAAATGCCTTCAGCCCTGCCCAATCACTGGACTTTGTTGAGTCCC
TGATGTCAGATGCGCAAAAGAAAGAGTTCCACGAGAGCCGTGAGTGTAACTTCGCCTTTGCCGCCAAGGACCTGGGACGT
TTCCGGGTCAGTGCTTTCTGGCAGCGTGAGTCTCCCGGTTGCGTAATGCGTCGTATCGAGACCAAAATTCCTGAGATGGA
AGACTTGAAACTGCCACCGGTGCTTAAAGATCTGGTGATGAGTAAACGCGGTCTTATCATTATGGTGGGTGGTACAGGTA
CGGGTAAATCGACGTCGCTGGCCGCCATGGTTGGGTATCGCAATGGCCATGAACGTGGTCATATCCTGACCATCGAAGAC
CCTGTGGAATTTGTGCACGATCACCGCAAGAGCATCATCACCCAGCGGGAAGTGGGGATCGATACCGAGTCCTTCGATGC
GGCGCTGAAAAGCTCGCTGCGTCAGGCCCCGGACGTGATCCTTATCGGTGAGATCCGCAGCCAGGAAACCATGGAATTTG
CGCTGTCATTTGCTGAGACAGGTCACCTGTGTATGGCTACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGTATC
ATGCACCTGGTGCCCGAAAGTAAGCACCAGCAGCTGTTGTTTGACCTGTCACTCAACCTGCGCGGTATTGTGGCACAGCA
ATTGGTACCCAAAGCCGATGGCACGGGGCGTCGTGCGGCCATTGAAATTCTGATTAACACCCCCCGTGTGTCGAGCATTA
TTCAGAAAAACGAGCTGCATTTGCTTAAAGACACCATGGCCAAGTCCAATGAGCAGGGGATGCAGACCTTTGACCAGGCG
CTGTTCAAGCTTTATCAGGAAGGTGAAATCACCTACGCCGATGCCTTGCACCATGCTGACTCGCCGAACGATCTGCGTTT
GATGATCAAACTGGCGGGCTCCGAAAGTACAGGTTCAGGCTTCCTGGAAGGCGTGACCCTGGACATGGATTGA

Upstream 100 bases:

>100_bases
CGAGCAGTGTCTGCAGAATCTGGTGAACCGTGGCCTCATCACTCGTGAGGACGCCATGGCCAAGAGCTCCAACAAACAGG
CAAACTTTTAAGGATTGATG

Downstream 100 bases:

>100_bases
CGCTCAGTCATGAAGAAAAACCAGGCGTATGCCTGGTTTTTTGATCGTTACGGCAACTTGTGGCGTAATCTGATGAGTAA
GAGTGCTTTCCGAAAATGGC

Product: twitching motility protein PilU

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 370; Mature: 370

Protein sequence:

>370_residues
MDVRPFLKVMVERKASDLFITAGFPPSAKIDGELRPLAENAFSPAQSLDFVESLMSDAQKKEFHESRECNFAFAAKDLGR
FRVSAFWQRESPGCVMRRIETKIPEMEDLKLPPVLKDLVMSKRGLIIMVGGTGTGKSTSLAAMVGYRNGHERGHILTIED
PVEFVHDHRKSIITQREVGIDTESFDAALKSSLRQAPDVILIGEIRSQETMEFALSFAETGHLCMATLHANNANQALDRI
MHLVPESKHQQLLFDLSLNLRGIVAQQLVPKADGTGRRAAIEILINTPRVSSIIQKNELHLLKDTMAKSNEQGMQTFDQA
LFKLYQEGEITYADALHHADSPNDLRLMIKLAGSESTGSGFLEGVTLDMD

Sequences:

>Translated_370_residues
MDVRPFLKVMVERKASDLFITAGFPPSAKIDGELRPLAENAFSPAQSLDFVESLMSDAQKKEFHESRECNFAFAAKDLGR
FRVSAFWQRESPGCVMRRIETKIPEMEDLKLPPVLKDLVMSKRGLIIMVGGTGTGKSTSLAAMVGYRNGHERGHILTIED
PVEFVHDHRKSIITQREVGIDTESFDAALKSSLRQAPDVILIGEIRSQETMEFALSFAETGHLCMATLHANNANQALDRI
MHLVPESKHQQLLFDLSLNLRGIVAQQLVPKADGTGRRAAIEILINTPRVSSIIQKNELHLLKDTMAKSNEQGMQTFDQA
LFKLYQEGEITYADALHHADSPNDLRLMIKLAGSESTGSGFLEGVTLDMD
>Mature_370_residues
MDVRPFLKVMVERKASDLFITAGFPPSAKIDGELRPLAENAFSPAQSLDFVESLMSDAQKKEFHESRECNFAFAAKDLGR
FRVSAFWQRESPGCVMRRIETKIPEMEDLKLPPVLKDLVMSKRGLIIMVGGTGTGKSTSLAAMVGYRNGHERGHILTIED
PVEFVHDHRKSIITQREVGIDTESFDAALKSSLRQAPDVILIGEIRSQETMEFALSFAETGHLCMATLHANNANQALDRI
MHLVPESKHQQLLFDLSLNLRGIVAQQLVPKADGTGRRAAIEILINTPRVSSIIQKNELHLLKDTMAKSNEQGMQTFDQA
LFKLYQEGEITYADALHHADSPNDLRLMIKLAGSESTGSGFLEGVTLDMD

Specific function: May be involved in pilus retraction [H]

COG id: COG5008

COG function: function code NU; Tfp pilus assembly protein, ATPase PilU

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=331, Percent_Identity=36.5558912386707, Blast_Score=221, Evalue=4e-59,
Organism=Escherichia coli, GI1789723, Length=156, Percent_Identity=41.6666666666667, Blast_Score=105, Evalue=7e-24,
Organism=Escherichia coli, GI1786296, Length=282, Percent_Identity=28.3687943262411, Blast_Score=91, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 41118; Mature: 41118

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVRPFLKVMVERKASDLFITAGFPPSAKIDGELRPLAENAFSPAQSLDFVESLMSDAQK
CCCHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHH
KEFHESRECNFAFAAKDLGRFRVSAFWQRESPGCVMRRIETKIPEMEDLKLPPVLKDLVM
HHHHHHCCCCEEEEHHHHHHHHHHHEECCCCCCHHHHHHHHHCCCHHCCCCCHHHHHHHH
SKRGLIIMVGGTGTGKSTSLAAMVGYRNGHERGHILTIEDPVEFVHDHRKSIITQREVGI
CCCCEEEEECCCCCCCCCCCEEEEECCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHCCC
DTESFDAALKSSLRQAPDVILIGEIRSQETMEFALSFAETGHLCMATLHANNANQALDRI
CHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHH
MHLVPESKHQQLLFDLSLNLRGIVAQQLVPKADGTGRRAAIEILINTPRVSSIIQKNELH
HHHCCCCCHHEEEEEECCCHHHHHHHHHCCCCCCCCCEEEEEEEEECCHHHHHHHHHHHH
LLKDTMAKSNEQGMQTFDQALFKLYQEGEITYADALHHADSPNDLRLMIKLAGSESTGSG
HHHHHHHCCCHHHHHHHHHHHHHHHHCCCEEHHHHHHCCCCCCCEEEEEEEECCCCCCCC
FLEGVTLDMD
EEEEEEEECC
>Mature Secondary Structure
MDVRPFLKVMVERKASDLFITAGFPPSAKIDGELRPLAENAFSPAQSLDFVESLMSDAQK
CCCHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHH
KEFHESRECNFAFAAKDLGRFRVSAFWQRESPGCVMRRIETKIPEMEDLKLPPVLKDLVM
HHHHHHCCCCEEEEHHHHHHHHHHHEECCCCCCHHHHHHHHHCCCHHCCCCCHHHHHHHH
SKRGLIIMVGGTGTGKSTSLAAMVGYRNGHERGHILTIEDPVEFVHDHRKSIITQREVGI
CCCCEEEEECCCCCCCCCCCEEEEECCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHCCC
DTESFDAALKSSLRQAPDVILIGEIRSQETMEFALSFAETGHLCMATLHANNANQALDRI
CHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHH
MHLVPESKHQQLLFDLSLNLRGIVAQQLVPKADGTGRRAAIEILINTPRVSSIIQKNELH
HHHCCCCCHHEEEEEECCCHHHHHHHHHCCCCCCCCCEEEEEEEEECCHHHHHHHHHHHH
LLKDTMAKSNEQGMQTFDQALFKLYQEGEITYADALHHADSPNDLRLMIKLAGSESTGSG
HHHHHHHCCCHHHHHHHHHHHHHHHHCCCEEHHHHHHCCCCCCCEEEEEEEECCCCCCCC
FLEGVTLDMD
EEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]