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The map label for this gene is glgX [H]

Identifier: 119775586

GI number: 119775586

Start: 2955205

End: 2957274

Strand: Reverse

Name: glgX [H]

Synonym: Sama_2452

Alternate gene names: 119775586

Gene position: 2957274-2955205 (Counterclockwise)

Preceding gene: 119775587

Following gene: 119775585

Centisome position: 68.68

GC content: 58.99

Gene sequence:

>2070_bases
ATGAGCGATTTGCACCGGCAATGCGGGGTTTGGCTCTTTAACGAAGATGGCGTCAGCAAGGCGCACTTCGGGGTGTTCAG
TCGCCATGCCAGCGCTGTGTTCCTGTGCCTATTCGATGAAGAGGGGCGGGAGTTTTCCTATCCCATGAAGCGCCGTGGTG
ATTTTTGGTCACTGACTCTGGATGGCATTGGCGAGGGGCAGGCCTATGGTTACCGCGCCGATGGTCCCTGGGCGCCGGCG
GCTGGTCACAGGTTTGACCCGGCTAAATTGCTCCTGGACCCCTATGCCCTGGCGATTGATGGTTTGCCAACGCTGGCGGT
TGCCCAGGCGGCTCAGGGCTCGGCCGGTCCACAGGACCCGCATTTTGCGCCCACTCCAGATGGGGCAGACAATGCCACCC
TGATGCCAAGATGCAGGGTGGTGGATGTGGCTGCCTTGCCGCCCTTTGTGCATCCCAAACCCGGTTATGCCATGGAAGAG
AGCGTGATTTACGAGCTTCATGTGAAGGGATTTTCCATGGGTTTTCCCGGGGTGAAGCCAGAGCACAGAGGCCGCTTTGC
CGCCCTCGCAGCACCTGAGGTTATCCAATATCTGAAGCGGCTGGGGGTCACGGCTCTGGAGCTGATGCCCTGCCAGCAGT
TTTTCGATGAGCCCTTTTTACATGCCAAAGGCCTGTCCAATTATTGGGGTTATAACAGCATCGGCTTTTTCGCCCCCCAC
AATGCCTATGGCGACATCCGCGAGTTCAGAGCCATGGTGGATGCCCTCCATGGCGCCGGTATCGAGGTCATTCTGGATGT
GGTCTACAACCACAGCGCCGAAGGCAATGCCATGGGGCCGGTATACAGCTTCAAGGGGCTGGACAATGCCTGCTATTACC
GCCTCACCGATACTGGCCATTACATCAATGATACCGGCTGCGGCAACACCCTGGATTTGGGGCAGCCCAGAGTGCTTGAG
CTGGTGATGGACTCACTGAGGTACTGGGCTTTGGTCGGCGGAGTCGACGGATTTCGCTTCGATTTGGCCGCCTGTCTGGG
CCGTATGCCCCATGGCTTTGATACCCACAGTGGCTTTTTTGCCGCGCTCGGGCAGGACCCTGTGCTTAAACACCTCAAGC
TTATCGCCGAGCCCTGGGATGTGGGCCCCGGTGGTTATCGCCTCGGCGACTTCCCCGCCGCCTTTGCCGAGTGGAATGAT
AAGTATCGCGATGCCATGCGCAGACTCTGGCGTGGCGATGAGCAAATTCTGGGTGAATTTGCCCGGCGTTTTCATGGCTC
GGCGGATCTCTTCGAGCCCTCCTTCCGTGGTCCCTGGGCCAGTGTCAATTTTATCGCCAGTCACGATGGTTTCACCCTGA
GGGATCTGCTGAGTTACGAGCAGCGCCACAACCTTGCCAACGGTGAAGACAATCGCGATGGCCATCAGGAAAACCTGAGC
CACAACTTCGGCGTGGAAGGAGAGGCCACCGAGCTGGTGGCGGCCCGAAAACACAAGGCCAGGGCGATGCTCGTTACCCT
GTTTTTCTCCTTTGGGATACCCATGCTGCGGGCCGGAGATGAGCTGTGGCAAACCCAGCAGGGCAACAACAATGCCTACT
GTCAGGACAATGCACTCACCTGGATTGATTGGGAAAGCGCCGACACTGAGCTCATCGACTTTGTGGCCGGGCTTATCCAT
ATCCGGCGGAAGCTGCCGCTGCTCGCGGCCACCCGTTATATCCATGGCAATAACCCGGACGATGGCCGCAAGGGTCCGTT
TCTGGCCTGGTTCAACGAGTCCGGCCTGCCCATGGCGGGCACCGACTGGCATCGCTGCGGCCCGCTTATCGCTGCGCTGG
GTGAGCATCATCCTCTGCGGGGCTCGGAGCTGTTACTGATTTTGATAAACCCCAGTCAGTCGGCGGTGGACATCAGTCTG
CCCCTGTTTGCCGTGTGGGAGCAATTGCACAGCCTGTGCGGTAACAGCGATATGCCGGCGCGGATAGAGGCGGGCGACAC
CCTGACGCTACCCGGCGTCAGTATCTGGATTTTTCACGGACACAGGGATGCCGACCCCGGCACTCAATAA

Upstream 100 bases:

>100_bases
GCACTAAGCCCAGCACTACGCCCAGCACTAAGCCCAGTGCCAAGTCGGTGGCCAAAGCACCGTCCAACGCGGCAAAAACC
AAAGCTGAGGATCCCGGCCT

Downstream 100 bases:

>100_bases
CGGAGACCACACCATGGCGACCAAACGACTGAAAGCGGCCGGACCCGAAACGGATACCGGCAGACCCCAGCCCTGTGAAC
CCTGCGATGCACTGCCGGCC

Product: glycogen operon protein

Products: NA

Alternate protein names: Glycogen operon protein GlgX [H]

Number of amino acids: Translated: 689; Mature: 688

Protein sequence:

>689_residues
MSDLHRQCGVWLFNEDGVSKAHFGVFSRHASAVFLCLFDEEGREFSYPMKRRGDFWSLTLDGIGEGQAYGYRADGPWAPA
AGHRFDPAKLLLDPYALAIDGLPTLAVAQAAQGSAGPQDPHFAPTPDGADNATLMPRCRVVDVAALPPFVHPKPGYAMEE
SVIYELHVKGFSMGFPGVKPEHRGRFAALAAPEVIQYLKRLGVTALELMPCQQFFDEPFLHAKGLSNYWGYNSIGFFAPH
NAYGDIREFRAMVDALHGAGIEVILDVVYNHSAEGNAMGPVYSFKGLDNACYYRLTDTGHYINDTGCGNTLDLGQPRVLE
LVMDSLRYWALVGGVDGFRFDLAACLGRMPHGFDTHSGFFAALGQDPVLKHLKLIAEPWDVGPGGYRLGDFPAAFAEWND
KYRDAMRRLWRGDEQILGEFARRFHGSADLFEPSFRGPWASVNFIASHDGFTLRDLLSYEQRHNLANGEDNRDGHQENLS
HNFGVEGEATELVAARKHKARAMLVTLFFSFGIPMLRAGDELWQTQQGNNNAYCQDNALTWIDWESADTELIDFVAGLIH
IRRKLPLLAATRYIHGNNPDDGRKGPFLAWFNESGLPMAGTDWHRCGPLIAALGEHHPLRGSELLLILINPSQSAVDISL
PLFAVWEQLHSLCGNSDMPARIEAGDTLTLPGVSIWIFHGHRDADPGTQ

Sequences:

>Translated_689_residues
MSDLHRQCGVWLFNEDGVSKAHFGVFSRHASAVFLCLFDEEGREFSYPMKRRGDFWSLTLDGIGEGQAYGYRADGPWAPA
AGHRFDPAKLLLDPYALAIDGLPTLAVAQAAQGSAGPQDPHFAPTPDGADNATLMPRCRVVDVAALPPFVHPKPGYAMEE
SVIYELHVKGFSMGFPGVKPEHRGRFAALAAPEVIQYLKRLGVTALELMPCQQFFDEPFLHAKGLSNYWGYNSIGFFAPH
NAYGDIREFRAMVDALHGAGIEVILDVVYNHSAEGNAMGPVYSFKGLDNACYYRLTDTGHYINDTGCGNTLDLGQPRVLE
LVMDSLRYWALVGGVDGFRFDLAACLGRMPHGFDTHSGFFAALGQDPVLKHLKLIAEPWDVGPGGYRLGDFPAAFAEWND
KYRDAMRRLWRGDEQILGEFARRFHGSADLFEPSFRGPWASVNFIASHDGFTLRDLLSYEQRHNLANGEDNRDGHQENLS
HNFGVEGEATELVAARKHKARAMLVTLFFSFGIPMLRAGDELWQTQQGNNNAYCQDNALTWIDWESADTELIDFVAGLIH
IRRKLPLLAATRYIHGNNPDDGRKGPFLAWFNESGLPMAGTDWHRCGPLIAALGEHHPLRGSELLLILINPSQSAVDISL
PLFAVWEQLHSLCGNSDMPARIEAGDTLTLPGVSIWIFHGHRDADPGTQ
>Mature_688_residues
SDLHRQCGVWLFNEDGVSKAHFGVFSRHASAVFLCLFDEEGREFSYPMKRRGDFWSLTLDGIGEGQAYGYRADGPWAPAA
GHRFDPAKLLLDPYALAIDGLPTLAVAQAAQGSAGPQDPHFAPTPDGADNATLMPRCRVVDVAALPPFVHPKPGYAMEES
VIYELHVKGFSMGFPGVKPEHRGRFAALAAPEVIQYLKRLGVTALELMPCQQFFDEPFLHAKGLSNYWGYNSIGFFAPHN
AYGDIREFRAMVDALHGAGIEVILDVVYNHSAEGNAMGPVYSFKGLDNACYYRLTDTGHYINDTGCGNTLDLGQPRVLEL
VMDSLRYWALVGGVDGFRFDLAACLGRMPHGFDTHSGFFAALGQDPVLKHLKLIAEPWDVGPGGYRLGDFPAAFAEWNDK
YRDAMRRLWRGDEQILGEFARRFHGSADLFEPSFRGPWASVNFIASHDGFTLRDLLSYEQRHNLANGEDNRDGHQENLSH
NFGVEGEATELVAARKHKARAMLVTLFFSFGIPMLRAGDELWQTQQGNNNAYCQDNALTWIDWESADTELIDFVAGLIHI
RRKLPLLAATRYIHGNNPDDGRKGPFLAWFNESGLPMAGTDWHRCGPLIAALGEHHPLRGSELLLILINPSQSAVDISLP
LFAVWEQLHSLCGNSDMPARIEAGDTLTLPGVSIWIFHGHRDADPGTQ

Specific function: Hydrolyzes the alpha-1,6-glucosidic linkages in glycogen which has first been partially depolymerized by phosphorylase. Shows only very little activity with native glycogen [H]

COG id: COG1523

COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Homo sapiens, GI189458812, Length=192, Percent_Identity=30.7291666666667, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI2367229, Length=629, Percent_Identity=45.6279809220986, Blast_Score=507, Evalue=1e-144,
Organism=Escherichia coli, GI1789839, Length=347, Percent_Identity=23.9193083573487, Blast_Score=65, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6320826, Length=208, Percent_Identity=29.3269230769231, Blast_Score=72, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR022844
- InterPro:   IPR011837
- InterPro:   IPR013783
- InterPro:   IPR014756 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48 [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 76293; Mature: 76162

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDLHRQCGVWLFNEDGVSKAHFGVFSRHASAVFLCLFDEEGREFSYPMKRRGDFWSLTL
CCCHHHHCCEEEECCCCCCHHHHHHHHCCCCEEEEEEECCCCCCCCCCHHHCCCEEEEEE
DGIGEGQAYGYRADGPWAPAAGHRFDPAKLLLDPYALAIDGLPTLAVAQAAQGSAGPQDP
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCHHHEECCCCHHHHHHHHCCCCCCCCC
HFAPTPDGADNATLMPRCRVVDVAALPPFVHPKPGYAMEESVIYELHVKGFSMGFPGVKP
CCCCCCCCCCCCEECCCEEEEEEECCCCCCCCCCCCCCCCCEEEEEEECEEECCCCCCCC
EHRGRFAALAAPEVIQYLKRLGVTALELMPCQQFFDEPFLHAKGLSNYWGYNSIGFFAPH
CCCCCEEEECCHHHHHHHHHCCCHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCCCCCC
NAYGDIREFRAMVDALHGAGIEVILDVVYNHSAEGNAMGPVYSFKGLDNACYYRLTDTGH
CCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCEEEECCCCCEEEEEECCCCC
YINDTGCGNTLDLGQPRVLELVMDSLRYWALVGGVDGFRFDLAACLGRMPHGFDTHSGFF
EECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCEE
AALGQDPVLKHLKLIAEPWDVGPGGYRLGDFPAAFAEWNDKYRDAMRRLWRGDEQILGEF
EECCCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHCHHHHHHHHHHHCCCHHHHHHH
ARRFHGSADLFEPSFRGPWASVNFIASHDGFTLRDLLSYEQRHNLANGEDNRDGHQENLS
HHHHCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHCC
HNFGVEGEATELVAARKHKARAMLVTLFFSFGIPMLRAGDELWQTQQGNNNAYCQDNALT
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCHHHHHHCCCCCCCEEECCCEE
WIDWESADTELIDFVAGLIHIRRKLPLLAATRYIHGNNPDDGRKGPFLAWFNESGLPMAG
EEECCCCCHHHHHHHHHHHHHHHHCCHHHHHHEECCCCCCCCCCCCEEEEECCCCCCCCC
TDWHRCGPLIAALGEHHPLRGSELLLILINPSQSAVDISLPLFAVWEQLHSLCGNSDMPA
CCHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCEEEECCHHHHHHHHHHHHHCCCCCCC
RIEAGDTLTLPGVSIWIFHGHRDADPGTQ
EEECCCEEECCCEEEEEEECCCCCCCCCC
>Mature Secondary Structure 
SDLHRQCGVWLFNEDGVSKAHFGVFSRHASAVFLCLFDEEGREFSYPMKRRGDFWSLTL
CCHHHHCCEEEECCCCCCHHHHHHHHCCCCEEEEEEECCCCCCCCCCHHHCCCEEEEEE
DGIGEGQAYGYRADGPWAPAAGHRFDPAKLLLDPYALAIDGLPTLAVAQAAQGSAGPQDP
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCHHHEECCCCHHHHHHHHCCCCCCCCC
HFAPTPDGADNATLMPRCRVVDVAALPPFVHPKPGYAMEESVIYELHVKGFSMGFPGVKP
CCCCCCCCCCCCEECCCEEEEEEECCCCCCCCCCCCCCCCCEEEEEEECEEECCCCCCCC
EHRGRFAALAAPEVIQYLKRLGVTALELMPCQQFFDEPFLHAKGLSNYWGYNSIGFFAPH
CCCCCEEEECCHHHHHHHHHCCCHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCCCCCC
NAYGDIREFRAMVDALHGAGIEVILDVVYNHSAEGNAMGPVYSFKGLDNACYYRLTDTGH
CCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCEEEECCCCCEEEEEECCCCC
YINDTGCGNTLDLGQPRVLELVMDSLRYWALVGGVDGFRFDLAACLGRMPHGFDTHSGFF
EECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCEE
AALGQDPVLKHLKLIAEPWDVGPGGYRLGDFPAAFAEWNDKYRDAMRRLWRGDEQILGEF
EECCCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHCHHHHHHHHHHHCCCHHHHHHH
ARRFHGSADLFEPSFRGPWASVNFIASHDGFTLRDLLSYEQRHNLANGEDNRDGHQENLS
HHHHCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHCC
HNFGVEGEATELVAARKHKARAMLVTLFFSFGIPMLRAGDELWQTQQGNNNAYCQDNALT
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCHHHHHHCCCCCCCEEECCCEE
WIDWESADTELIDFVAGLIHIRRKLPLLAATRYIHGNNPDDGRKGPFLAWFNESGLPMAG
EEECCCCCHHHHHHHHHHHHHHHHCCHHHHHHEECCCCCCCCCCCCEEEEECCCCCCCCC
TDWHRCGPLIAALGEHHPLRGSELLLILINPSQSAVDISLPLFAVWEQLHSLCGNSDMPA
CCHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCEEEECCHHHHHHHHHHHHHCCCCCCC
RIEAGDTLTLPGVSIWIFHGHRDADPGTQ
EEECCCEEECCCEEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Glycosidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA