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The map label for this gene is leuB [H]

Identifier: 119775557

GI number: 119775557

Start: 2917552

End: 2918562

Strand: Direct

Name: leuB [H]

Synonym: Sama_2423

Alternate gene names: 119775557

Gene position: 2917552-2918562 (Clockwise)

Preceding gene: 119775555

Following gene: 119775558

Centisome position: 67.75

GC content: 54.2

Gene sequence:

>1011_bases
ATGTCTAAAAGAACGATTACCGTAATCCCAGGTGATGGGATCGGCCCAAGCATCATCGACTCCGCTCTGAAAATCCTCGA
TAAGGCGGGTTGCGACTTCGAATATGAATTTGCCGATGCTGGCCTGACGGCTCTGGAAAAGCATGGTGAACTGCTGCCTC
AGCGTACGCTGGATCTTATCGAAAAGAACCGTATTACCCTGAAGGGTCCACTGACTACTCCCGTTGGTGAAGGCTTTACC
TCCATCAACGTTACCCTGCGTAAAAAGTTCAGCCTGTACGCCAACGTACGTCCAGTGAACTCTTTCAAGGGAACCCAGGC
CAGATACGAAAATATCGACATCATCACTGTCCGTGAAAACACCGAAGGCATGTACTCCGGTTACGGCCAGAAAGTATCAG
ATGACGGCAGCACTGCCGAAGCCACCAGCATCATCACCCGTCAGGGTGCTGAGCAGATCACCACTTTTGCCTACGAGCTG
GCCCGCAAGGAAGGCCGTAAGAAAGTGACCATAGTGCACAAGGCCAATATCATGAAGTCCACCTCAGGCCTGTTCCTGAA
AGTGGCCCGTGAAGTGAGCCAGCGCTATCCCGACATCACCACCGAAGAAATGATTGTGGACGCCACCTGTATGAAGCTGG
TGATGAACCCGGAAAACTTCGATGTGATTGTAACTACCAACCTGTTTGGTGACATTCTGTCTGACCTCTGTGCCGGTCTG
GTGGGTGGTCTGGGCATGGCGCCCGGTGCCAACATCGGTGCCAGTGCGGCTATTTTTGAAGCCGTACACGGCAGTGCCCC
GGATATCGCCGGTAAAAACCTGGCTAACCCCACTTCCGTGATCCTGGCCTCTGTACAGATGCTGGAATACTTGGGCATGG
CTGACAAAGCAGCGGCTATCCGCAATGCCGTGGCGGCTGTGATTGAAGAAGGCGACCGTACTACCCGCGATCTCGGCGGC
ACCCACGGTACCACCGACTTCACTCAAGCCGTCCTCGACAGACTCGCCTGA

Upstream 100 bases:

>100_bases
GTGTTTAAACTAATCGAGACATAAATCACATAATTCCAGTCTTGTTTCCGCTTGCGGAAGCCGACAATTGCAGTAACCAC
CAACCGTGGAAGGAATATCC

Downstream 100 bases:

>100_bases
GAGCCCAGCGTCATCTGACGCAGCAAACAGTGAAAAAGGATGAGCCAAGGCTCATCCTTTTTATTTTCCCACAATCGCCA
ATACCCAATGCCCATGCAAA

Product: isocitrate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; IMDH; Beta-IPM dehydrogenase [H]

Number of amino acids: Translated: 336; Mature: 335

Protein sequence:

>336_residues
MSKRTITVIPGDGIGPSIIDSALKILDKAGCDFEYEFADAGLTALEKHGELLPQRTLDLIEKNRITLKGPLTTPVGEGFT
SINVTLRKKFSLYANVRPVNSFKGTQARYENIDIITVRENTEGMYSGYGQKVSDDGSTAEATSIITRQGAEQITTFAYEL
ARKEGRKKVTIVHKANIMKSTSGLFLKVAREVSQRYPDITTEEMIVDATCMKLVMNPENFDVIVTTNLFGDILSDLCAGL
VGGLGMAPGANIGASAAIFEAVHGSAPDIAGKNLANPTSVILASVQMLEYLGMADKAAAIRNAVAAVIEEGDRTTRDLGG
THGTTDFTQAVLDRLA

Sequences:

>Translated_336_residues
MSKRTITVIPGDGIGPSIIDSALKILDKAGCDFEYEFADAGLTALEKHGELLPQRTLDLIEKNRITLKGPLTTPVGEGFT
SINVTLRKKFSLYANVRPVNSFKGTQARYENIDIITVRENTEGMYSGYGQKVSDDGSTAEATSIITRQGAEQITTFAYEL
ARKEGRKKVTIVHKANIMKSTSGLFLKVAREVSQRYPDITTEEMIVDATCMKLVMNPENFDVIVTTNLFGDILSDLCAGL
VGGLGMAPGANIGASAAIFEAVHGSAPDIAGKNLANPTSVILASVQMLEYLGMADKAAAIRNAVAAVIEEGDRTTRDLGG
THGTTDFTQAVLDRLA
>Mature_335_residues
SKRTITVIPGDGIGPSIIDSALKILDKAGCDFEYEFADAGLTALEKHGELLPQRTLDLIEKNRITLKGPLTTPVGEGFTS
INVTLRKKFSLYANVRPVNSFKGTQARYENIDIITVRENTEGMYSGYGQKVSDDGSTAEATSIITRQGAEQITTFAYELA
RKEGRKKVTIVHKANIMKSTSGLFLKVAREVSQRYPDITTEEMIVDATCMKLVMNPENFDVIVTTNLFGDILSDLCAGLV
GGLGMAPGANIGASAAIFEAVHGSAPDIAGKNLANPTSVILASVQMLEYLGMADKAAAIRNAVAAVIEEGDRTTRDLGGT
HGTTDFTQAVLDRLA

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=335, Percent_Identity=46.865671641791, Blast_Score=321, Evalue=6e-88,
Organism=Homo sapiens, GI4758582, Length=336, Percent_Identity=41.6666666666667, Blast_Score=248, Evalue=7e-66,
Organism=Homo sapiens, GI28178838, Length=310, Percent_Identity=42.9032258064516, Blast_Score=237, Evalue=1e-62,
Organism=Homo sapiens, GI28178821, Length=336, Percent_Identity=39.5833333333333, Blast_Score=236, Evalue=2e-62,
Organism=Homo sapiens, GI28178816, Length=336, Percent_Identity=39.2857142857143, Blast_Score=232, Evalue=5e-61,
Organism=Homo sapiens, GI28178819, Length=198, Percent_Identity=46.969696969697, Blast_Score=174, Evalue=8e-44,
Organism=Escherichia coli, GI87081683, Length=340, Percent_Identity=38.2352941176471, Blast_Score=204, Evalue=1e-53,
Organism=Escherichia coli, GI1788101, Length=356, Percent_Identity=34.2696629213483, Blast_Score=196, Evalue=2e-51,
Organism=Escherichia coli, GI1787381, Length=360, Percent_Identity=34.1666666666667, Blast_Score=188, Evalue=5e-49,
Organism=Caenorhabditis elegans, GI71986051, Length=332, Percent_Identity=46.6867469879518, Blast_Score=324, Evalue=5e-89,
Organism=Caenorhabditis elegans, GI25144293, Length=338, Percent_Identity=40.8284023668639, Blast_Score=245, Evalue=2e-65,
Organism=Caenorhabditis elegans, GI17550882, Length=342, Percent_Identity=39.766081871345, Blast_Score=237, Evalue=5e-63,
Organism=Caenorhabditis elegans, GI17505779, Length=335, Percent_Identity=39.7014925373134, Blast_Score=231, Evalue=6e-61,
Organism=Saccharomyces cerevisiae, GI6324709, Length=343, Percent_Identity=46.064139941691, Blast_Score=297, Evalue=1e-81,
Organism=Saccharomyces cerevisiae, GI6324291, Length=337, Percent_Identity=41.839762611276, Blast_Score=250, Evalue=2e-67,
Organism=Saccharomyces cerevisiae, GI6322097, Length=349, Percent_Identity=39.2550143266476, Blast_Score=224, Evalue=1e-59,
Organism=Saccharomyces cerevisiae, GI6319830, Length=363, Percent_Identity=30.5785123966942, Blast_Score=146, Evalue=4e-36,
Organism=Drosophila melanogaster, GI24643270, Length=332, Percent_Identity=44.8795180722892, Blast_Score=321, Evalue=5e-88,
Organism=Drosophila melanogaster, GI24643268, Length=332, Percent_Identity=44.8795180722892, Blast_Score=321, Evalue=5e-88,
Organism=Drosophila melanogaster, GI24661184, Length=340, Percent_Identity=42.3529411764706, Blast_Score=286, Evalue=2e-77,
Organism=Drosophila melanogaster, GI161078633, Length=337, Percent_Identity=37.9821958456973, Blast_Score=228, Evalue=3e-60,
Organism=Drosophila melanogaster, GI24650122, Length=337, Percent_Identity=37.9821958456973, Blast_Score=228, Evalue=3e-60,
Organism=Drosophila melanogaster, GI161078637, Length=337, Percent_Identity=37.9821958456973, Blast_Score=228, Evalue=4e-60,
Organism=Drosophila melanogaster, GI161078635, Length=337, Percent_Identity=37.9821958456973, Blast_Score=228, Evalue=4e-60,
Organism=Drosophila melanogaster, GI281362242, Length=342, Percent_Identity=41.5204678362573, Blast_Score=227, Evalue=1e-59,
Organism=Drosophila melanogaster, GI24648872, Length=342, Percent_Identity=41.5204678362573, Blast_Score=227, Evalue=1e-59,
Organism=Drosophila melanogaster, GI161078639, Length=332, Percent_Identity=37.6506024096386, Blast_Score=224, Evalue=4e-59,
Organism=Drosophila melanogaster, GI20130355, Length=331, Percent_Identity=28.3987915407855, Blast_Score=167, Evalue=8e-42,
Organism=Drosophila melanogaster, GI45550436, Length=232, Percent_Identity=25.4310344827586, Blast_Score=87, Evalue=1e-17,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR011828 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 35983; Mature: 35851

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKRTITVIPGDGIGPSIIDSALKILDKAGCDFEYEFADAGLTALEKHGELLPQRTLDLI
CCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEHHHCCHHHHHHHHHHCHHHHHHHH
EKNRITLKGPLTTPVGEGFTSINVTLRKKFSLYANVRPVNSFKGTQARYENIDIITVREN
HCCCEEEECCCCCCCCCCCEEEEEEEEEEEEEEEECCCCCCCCCCCHHHCCEEEEEEECC
TEGMYSGYGQKVSDDGSTAEATSIITRQGAEQITTFAYELARKEGRKKVTIVHKANIMKS
CCHHHHCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEEEEECCHHHC
TSGLFLKVAREVSQRYPDITTEEMIVDATCMKLVMNPENFDVIVTTNLFGDILSDLCAGL
CCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHH
VGGLGMAPGANIGASAAIFEAVHGSAPDIAGKNLANPTSVILASVQMLEYLGMADKAAAI
HCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHH
RNAVAAVIEEGDRTTRDLGGTHGTTDFTQAVLDRLA
HHHHHHHHHCCCCHHHHCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
SKRTITVIPGDGIGPSIIDSALKILDKAGCDFEYEFADAGLTALEKHGELLPQRTLDLI
CCCEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEHHHCCHHHHHHHHHHCHHHHHHHH
EKNRITLKGPLTTPVGEGFTSINVTLRKKFSLYANVRPVNSFKGTQARYENIDIITVREN
HCCCEEEECCCCCCCCCCCEEEEEEEEEEEEEEEECCCCCCCCCCCHHHCCEEEEEEECC
TEGMYSGYGQKVSDDGSTAEATSIITRQGAEQITTFAYELARKEGRKKVTIVHKANIMKS
CCHHHHCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEEEEECCHHHC
TSGLFLKVAREVSQRYPDITTEEMIVDATCMKLVMNPENFDVIVTTNLFGDILSDLCAGL
CCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHH
VGGLGMAPGANIGASAAIFEAVHGSAPDIAGKNLANPTSVILASVQMLEYLGMADKAAAI
HCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHH
RNAVAAVIEEGDRTTRDLGGTHGTTDFTQAVLDRLA
HHHHHHHHHCCCCHHHHCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]