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The map label for this gene is ycdT [H]

Identifier: 119775516

GI number: 119775516

Start: 2862794

End: 2864743

Strand: Reverse

Name: ycdT [H]

Synonym: Sama_2382

Alternate gene names: 119775516

Gene position: 2864743-2862794 (Counterclockwise)

Preceding gene: 119775517

Following gene: 119775515

Centisome position: 66.53

GC content: 52.1

Gene sequence:

>1950_bases
ATGACCTGTTACCGTGCTGCACTCGTGTGTGCGCTTTGGCTTCTTTTGCCGTGGCTTGCCATGGCAAATGGAGACTATCG
CGCAATGCTGAATGAGCTTATAAACATGCAGCACGGTCCTTCTGAAAGAAAAACAGATCTCATTAATACCTTAAAGACAG
CCGAAAAGGCGCTCGATGCGGAAGAACTCGGTCAGTTGAAAATGATCCTCGGCAGAGAGCAATTATATGAGGGGGCCTAT
CAAGCGGGTGTGAATGATCTGAAGGTGGCCGAGTCACAATTGTTTTCATCCCGACATTTGCATTTGGTTTATGGCTACCT
TGCCACAGGTTATCAGCTGCTGGGTGATTACGAATCCTCCCTTCGTTACCTAAAACTTGGGCTTGAACTGGTAAAAACAG
TTTCAGACAAAGACGTACAGCGCAACGCGTACATTCGGGCGGCGAGTCTCTTCTACCAGCTCGGCTTTTATGAAGAGGTG
GGGCTTTATGCCCAGAGGGCGCTTCAGCTAGCTGATGATGCCAATACCAAGGATATCTGTTACGCCAACCTCTATATTGC
CGCTTATATTCATGAGTCGGGAGAACTGCCCCGTGCTGCAGAGGCGTTCCAGCGAACGGGCGATTATTGTCAGGAGCATG
GCTGGCGGCTCATTACCGTGATGACCATTAAGTCACGTGGACTTATCAGCCATGAGTCAAACCTGATTGAGGATGCCATC
ACCCTGATGGAGCAGGCGCTGGCAGAGTACCGCACCCTTGGTTTTCAGCTGGAGATTGCCAGTACCCAAAGCGCATTGGC
AAAGGCCTATCTCGACATTGGCGATTGGGAGAAGGCGGAGGCTGCCGCTGTCGAAGCTATCGACAGCAAAGGTGCCGACA
ATAAGGCCCTGAAAGACGCCTGGTATGTGATGGCCACACTGAAGGCGAGAAGTAACGACTTTGTGACTGCCTATGAAGCC
CAAAAGCATGAAAATCATTATGCCAATGAACTGCTGAACGAAACCAAGGCCCGTGAAATGGCCTACCAGGCGGCCAAGTT
TAATTTTGTTGAGCAGCAGCGGGAAATCGCTTTATTGAACAGTGAAAGGGACAGCTATCTGGTCAGGCAGGAAACTATCT
CCCGGGAGCATTCCGGGTCTTTGATGATTTCAACTGTGCTGGCGGGTATTACCTTATTTTTATCTGTGTTTCTGGCAACC
GGTTTGTCTCAACGTAACAAGTACATGCGCCTGGCACAGCGCGACGGCCTGACCGGAACCTACAATCGTGCCACGGGGCA
GGAAAAAGGTGAAAATGCGCTGATTGGCTGTATGGCGCGAGGTGAGCCGACGGCGGCTGTCTTGCTCGATTTGGATAATT
TCAAGCGGATCAATGATGAGTTCGGCCATGCTACCGGCGACTGGACCCTGAAAAAGGTCGTGGAAGTGATTCGGCCACAG
TTGGCCGATGACCATATTCTGTGCCGCTTTGGCGGTGAGGAGTTTATGTTGATCCTGCCCGGGCTTTCGGGCGCACAGGC
GGTGGAAGTGGCTGAGCGTTGCCGAGAGGCCATTGCCGGGGTCAATACCCATTATTCGGGTCACAGCTTTAAACTGACCG
CCAGTTTTGGGGTGACCGAGTGGCAGCATGGTGATTTAAGCCTTGACCCCATGATCAAGCGCGCCGATTTGGCCTTATAC
CGGGCCAAACATCTGGGGCGTAACCGGGTCGTCAGCTATGGCTTTGCGGAGCTTGAGGGAGGCAATAATCAGTCTTTTGC
CGATACCCAGGAAAACTCGTCAGCGCAAACCAGAAATCAAACACCATCATCAATCAGCACTGCCCAATCGCCTGCGGCGT
CAGAGGGTAAAATCGAAGAAGAGTCTTCGAAAGTGAGCGCTGCTTATGCGGTATCCCCGGAGCAATCCCAGGTCCTCGCT
GAGTCGCCTGATAAGGTCGCAACACCCTAA

Upstream 100 bases:

>100_bases
TCAAGCCATCATGAGTGGTGATAAAGCCAAACTCAAGGCCCTTGGTGGAGAAGAGCAGTACCAGTCTCTGGTTGTGATTT
ATAACGGGAATGATGCGTAA

Downstream 100 bases:

>100_bases
GATGACCGCAAGAAAACAGCTATCTACTCATTGGCAAGGTTCCTCTTGGGTATTGAGTATCTGGCGCACTTTACATTCAC
TCTACAGCCTAGCTTTAAAG

Product: GGDEF family protein

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 649; Mature: 648

Protein sequence:

>649_residues
MTCYRAALVCALWLLLPWLAMANGDYRAMLNELINMQHGPSERKTDLINTLKTAEKALDAEELGQLKMILGREQLYEGAY
QAGVNDLKVAESQLFSSRHLHLVYGYLATGYQLLGDYESSLRYLKLGLELVKTVSDKDVQRNAYIRAASLFYQLGFYEEV
GLYAQRALQLADDANTKDICYANLYIAAYIHESGELPRAAEAFQRTGDYCQEHGWRLITVMTIKSRGLISHESNLIEDAI
TLMEQALAEYRTLGFQLEIASTQSALAKAYLDIGDWEKAEAAAVEAIDSKGADNKALKDAWYVMATLKARSNDFVTAYEA
QKHENHYANELLNETKAREMAYQAAKFNFVEQQREIALLNSERDSYLVRQETISREHSGSLMISTVLAGITLFLSVFLAT
GLSQRNKYMRLAQRDGLTGTYNRATGQEKGENALIGCMARGEPTAAVLLDLDNFKRINDEFGHATGDWTLKKVVEVIRPQ
LADDHILCRFGGEEFMLILPGLSGAQAVEVAERCREAIAGVNTHYSGHSFKLTASFGVTEWQHGDLSLDPMIKRADLALY
RAKHLGRNRVVSYGFAELEGGNNQSFADTQENSSAQTRNQTPSSISTAQSPAASEGKIEEESSKVSAAYAVSPEQSQVLA
ESPDKVATP

Sequences:

>Translated_649_residues
MTCYRAALVCALWLLLPWLAMANGDYRAMLNELINMQHGPSERKTDLINTLKTAEKALDAEELGQLKMILGREQLYEGAY
QAGVNDLKVAESQLFSSRHLHLVYGYLATGYQLLGDYESSLRYLKLGLELVKTVSDKDVQRNAYIRAASLFYQLGFYEEV
GLYAQRALQLADDANTKDICYANLYIAAYIHESGELPRAAEAFQRTGDYCQEHGWRLITVMTIKSRGLISHESNLIEDAI
TLMEQALAEYRTLGFQLEIASTQSALAKAYLDIGDWEKAEAAAVEAIDSKGADNKALKDAWYVMATLKARSNDFVTAYEA
QKHENHYANELLNETKAREMAYQAAKFNFVEQQREIALLNSERDSYLVRQETISREHSGSLMISTVLAGITLFLSVFLAT
GLSQRNKYMRLAQRDGLTGTYNRATGQEKGENALIGCMARGEPTAAVLLDLDNFKRINDEFGHATGDWTLKKVVEVIRPQ
LADDHILCRFGGEEFMLILPGLSGAQAVEVAERCREAIAGVNTHYSGHSFKLTASFGVTEWQHGDLSLDPMIKRADLALY
RAKHLGRNRVVSYGFAELEGGNNQSFADTQENSSAQTRNQTPSSISTAQSPAASEGKIEEESSKVSAAYAVSPEQSQVLA
ESPDKVATP
>Mature_648_residues
TCYRAALVCALWLLLPWLAMANGDYRAMLNELINMQHGPSERKTDLINTLKTAEKALDAEELGQLKMILGREQLYEGAYQ
AGVNDLKVAESQLFSSRHLHLVYGYLATGYQLLGDYESSLRYLKLGLELVKTVSDKDVQRNAYIRAASLFYQLGFYEEVG
LYAQRALQLADDANTKDICYANLYIAAYIHESGELPRAAEAFQRTGDYCQEHGWRLITVMTIKSRGLISHESNLIEDAIT
LMEQALAEYRTLGFQLEIASTQSALAKAYLDIGDWEKAEAAAVEAIDSKGADNKALKDAWYVMATLKARSNDFVTAYEAQ
KHENHYANELLNETKAREMAYQAAKFNFVEQQREIALLNSERDSYLVRQETISREHSGSLMISTVLAGITLFLSVFLATG
LSQRNKYMRLAQRDGLTGTYNRATGQEKGENALIGCMARGEPTAAVLLDLDNFKRINDEFGHATGDWTLKKVVEVIRPQL
ADDHILCRFGGEEFMLILPGLSGAQAVEVAERCREAIAGVNTHYSGHSFKLTASFGVTEWQHGDLSLDPMIKRADLALYR
AKHLGRNRVVSYGFAELEGGNNQSFADTQENSSAQTRNQTPSSISTAQSPAASEGKIEEESSKVSAAYAVSPEQSQVLAE
SPDKVATP

Specific function: A probable diguanylate cyclase, overexpression leads to a strong repression of swimming; swimming returns to normal when residues 359-360 are both mutated to Ala. Overexpression also leads to a 20-fold increase in c-di-GMP levels in vivo. Cyclic-di- GMP i

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=181, Percent_Identity=34.2541436464088, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI87082007, Length=229, Percent_Identity=34.4978165938865, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI87081881, Length=161, Percent_Identity=36.6459627329193, Blast_Score=94, Evalue=4e-20,
Organism=Escherichia coli, GI1786584, Length=198, Percent_Identity=30.8080808080808, Blast_Score=90, Evalue=6e-19,
Organism=Escherichia coli, GI1788381, Length=183, Percent_Identity=30.6010928961749, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI145693134, Length=157, Percent_Identity=32.484076433121, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1787802, Length=237, Percent_Identity=28.6919831223629, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1788085, Length=185, Percent_Identity=34.0540540540541, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1787816, Length=160, Percent_Identity=33.125, Blast_Score=74, Evalue=4e-14,
Organism=Escherichia coli, GI1787541, Length=168, Percent_Identity=33.3333333333333, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI87081977, Length=155, Percent_Identity=30.3225806451613, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI1788956, Length=162, Percent_Identity=32.7160493827161, Blast_Score=67, Evalue=4e-12,
Organism=Escherichia coli, GI1787056, Length=128, Percent_Identity=34.375, Blast_Score=66, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 72031; Mature: 71900

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTCYRAALVCALWLLLPWLAMANGDYRAMLNELINMQHGPSERKTDLINTLKTAEKALDA
CCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCH
EELGQLKMILGREQLYEGAYQAGVNDLKVAESQLFSSRHLHLVYGYLATGYQLLGDYESS
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
LRYLKLGLELVKTVSDKDVQRNAYIRAASLFYQLGFYEEVGLYAQRALQLADDANTKDIC
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCEE
YANLYIAAYIHESGELPRAAEAFQRTGDYCQEHGWRLITVMTIKSRGLISHESNLIEDAI
EEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCHHHHHHHHH
TLMEQALAEYRTLGFQLEIASTQSALAKAYLDIGDWEKAEAAAVEAIDSKGADNKALKDA
HHHHHHHHHHHHCCEEEEECCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHHHHH
WYVMATLKARSNDFVTAYEAQKHENHYANELLNETKAREMAYQAAKFNFVEQQREIALLN
HHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEC
SERDSYLVRQETISREHSGSLMISTVLAGITLFLSVFLATGLSQRNKYMRLAQRDGLTGT
CCCCHHHHHHHHHCHHCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCC
YNRATGQEKGENALIGCMARGEPTAAVLLDLDNFKRINDEFGHATGDWTLKKVVEVIRPQ
CCCCCCCCCCCCCEEEEEECCCCCEEEEEECHHHHHHHHHHCCCCCCHHHHHHHHHHCCC
LADDHILCRFGGEEFMLILPGLSGAQAVEVAERCREAIAGVNTHYSGHSFKLTASFGVTE
CCCCCEEEEECCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCC
WQHGDLSLDPMIKRADLALYRAKHLGRNRVVSYGFAELEGGNNQSFADTQENSSAQTRNQ
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEECCHHEECCCCCCCCCCCCCCCCCHHCCC
TPSSISTAQSPAASEGKIEEESSKVSAAYAVSPEQSQVLAESPDKVATP
CCCCHHHCCCCCCCCCCCCHHHCCEEEEEEECCCHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TCYRAALVCALWLLLPWLAMANGDYRAMLNELINMQHGPSERKTDLINTLKTAEKALDA
CHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCH
EELGQLKMILGREQLYEGAYQAGVNDLKVAESQLFSSRHLHLVYGYLATGYQLLGDYESS
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
LRYLKLGLELVKTVSDKDVQRNAYIRAASLFYQLGFYEEVGLYAQRALQLADDANTKDIC
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCEE
YANLYIAAYIHESGELPRAAEAFQRTGDYCQEHGWRLITVMTIKSRGLISHESNLIEDAI
EEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCHHHHHHHHH
TLMEQALAEYRTLGFQLEIASTQSALAKAYLDIGDWEKAEAAAVEAIDSKGADNKALKDA
HHHHHHHHHHHHCCEEEEECCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHHHHH
WYVMATLKARSNDFVTAYEAQKHENHYANELLNETKAREMAYQAAKFNFVEQQREIALLN
HHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEC
SERDSYLVRQETISREHSGSLMISTVLAGITLFLSVFLATGLSQRNKYMRLAQRDGLTGT
CCCCHHHHHHHHHCHHCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCC
YNRATGQEKGENALIGCMARGEPTAAVLLDLDNFKRINDEFGHATGDWTLKKVVEVIRPQ
CCCCCCCCCCCCCEEEEEECCCCCEEEEEECHHHHHHHHHHCCCCCCHHHHHHHHHHCCC
LADDHILCRFGGEEFMLILPGLSGAQAVEVAERCREAIAGVNTHYSGHSFKLTASFGVTE
CCCCCEEEEECCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCC
WQHGDLSLDPMIKRADLALYRAKHLGRNRVVSYGFAELEGGNNQSFADTQENSSAQTRNQ
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEECCHHEECCCCCCCCCCCCCCCCCHHCCC
TPSSISTAQSPAASEGKIEEESSKVSAAYAVSPEQSQVLAESPDKVATP
CCCCHHHCCCCCCCCCCCCHHHCCEEEEEEECCCHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]