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The map label for this gene is aceA [H]

Identifier: 119775514

GI number: 119775514

Start: 2858428

End: 2859747

Strand: Reverse

Name: aceA [H]

Synonym: Sama_2380

Alternate gene names: 119775514

Gene position: 2859747-2858428 (Counterclockwise)

Preceding gene: 119775515

Following gene: 119775513

Centisome position: 66.41

GC content: 56.14

Gene sequence:

>1320_bases
ATGAGCAAGTACATCAGCCGCCAGGAACAAATTGACGCACTGAAAAAGGACTGGGCCGAAAACCCACGCTGGAAAGGTGT
GCGCCGTCCTTATACTGCCGAAGAAGTGGTTTCGCTGCGCGGCTCTTTTGTACCGGAGAATACAGTGGCCCAGCGCGGCG
CAGCCAAACTGTGGGACCTGGTCAACGGTGGTGCCAAAAAAGGTTATGTAAACTCCCTCGGTGCCCTGACCGGTGGTCAG
GCGGTACAGCAGGCCAAGGCCGGCATTGAAGCTATCTATTTGTCTGGCTGGCAGGTAGCCGCCGATGCCAACCTGGCCGG
TACCATGTACCCAGACCAATCTCTGTACCCCGCCAACTCTGTGCCTGCTGTGGTTGGTCGTATCAATAACTCATTCCGCC
GCGCCGACCAGATCCAATGGAGCAACGGCGTAGGTCCGGAAGATGACAAGTTCGTTGATTACTTCCTGCCAATCGTGGCG
GATGCCGAAGCCGGCTTTGGTGGTGTACTCAATGCTTACGAGTTGATGAAGTCTATGATTGATGCCGGTGCCGCCGGTGT
GCACTTTGAAGATCAGCTGGCATCGGTAAAGAAATGCGGTCACATGGGCGGCAAAGTGTTGGTGCCCACTCAGGAAGCGG
TACAAAAACTGGTTGCGGCCCGTTTGGCGGCTGACGTATCTGGCGTAGAGACTCTGGTTATTGCCCGTACCGACGCCAAT
GCTGCCGATCTGCTGACCTCGGATTGCGACCCATACGACAGCGACTTCATCACAGGTGAGCGTACCAGCGAAGGTTTCTA
CCGTGTCCGTGCCGGTCTGGACCAGGCCATCGCCCGTGGTTTGGCTTACGCTCCATACGCCGACCTCATCTGGTGTGAAA
CTGCCAAGCCGGACCTGGAAGAAGCCCGCCGCTTCGCCGAGGCCATCCATGCCAAGTACCCGGATCAGCTGCTGGCCTAT
AACTGCTCACCTTCTTTCAACTGGAAGAAGAATCTGGACGACGCCACTATCGCCAAGTTCCAGCAGGCCTTGTCTGACAT
GGGCTACAAGTATCAGTTCATCACCCTGGCCGGTATCCACAATATGTGGTTCAACATGTTCGACCTGGCGTACGACTACG
CCCGCGGTGAAGGCATGAAGCACTATGTAGAGAAGGTGCAGGAAGCCGAGTTTGCTGCCGCCAGTAAGGGATATACCTTT
GTGGCGCACCAGCAGGAAGTGGGCACCGGCTACTTCGACAAGATGACCAATATCATTCAGGGCGGTGCCTCTTCTGTGAC
TGCACTGACCGGTTCCACTGAGGAAGAACAGTTCCACTGA

Upstream 100 bases:

>100_bases
GCTGACCAAACAAGAACACTGATTAATCCCTGAAAACATAGAACATCTTACCCGTCACATGCGTGACGGGCAGACCCTAA
CACCCCAAAGGAGAGTCACC

Downstream 100 bases:

>100_bases
TAGTCGTTGCTCCCTTTTACCCTGCACGGTTTCCTACGTGCCGTGCGGGGCTTTTCTCTCCCTTTGGGTGAGACTTCCCA
TGCTGTTGTTTTCAACCACG

Product: isocitrate lyase

Products: NA

Alternate protein names: ICL; Isocitrase; Isocitratase [H]

Number of amino acids: Translated: 439; Mature: 438

Protein sequence:

>439_residues
MSKYISRQEQIDALKKDWAENPRWKGVRRPYTAEEVVSLRGSFVPENTVAQRGAAKLWDLVNGGAKKGYVNSLGALTGGQ
AVQQAKAGIEAIYLSGWQVAADANLAGTMYPDQSLYPANSVPAVVGRINNSFRRADQIQWSNGVGPEDDKFVDYFLPIVA
DAEAGFGGVLNAYELMKSMIDAGAAGVHFEDQLASVKKCGHMGGKVLVPTQEAVQKLVAARLAADVSGVETLVIARTDAN
AADLLTSDCDPYDSDFITGERTSEGFYRVRAGLDQAIARGLAYAPYADLIWCETAKPDLEEARRFAEAIHAKYPDQLLAY
NCSPSFNWKKNLDDATIAKFQQALSDMGYKYQFITLAGIHNMWFNMFDLAYDYARGEGMKHYVEKVQEAEFAAASKGYTF
VAHQQEVGTGYFDKMTNIIQGGASSVTALTGSTEEEQFH

Sequences:

>Translated_439_residues
MSKYISRQEQIDALKKDWAENPRWKGVRRPYTAEEVVSLRGSFVPENTVAQRGAAKLWDLVNGGAKKGYVNSLGALTGGQ
AVQQAKAGIEAIYLSGWQVAADANLAGTMYPDQSLYPANSVPAVVGRINNSFRRADQIQWSNGVGPEDDKFVDYFLPIVA
DAEAGFGGVLNAYELMKSMIDAGAAGVHFEDQLASVKKCGHMGGKVLVPTQEAVQKLVAARLAADVSGVETLVIARTDAN
AADLLTSDCDPYDSDFITGERTSEGFYRVRAGLDQAIARGLAYAPYADLIWCETAKPDLEEARRFAEAIHAKYPDQLLAY
NCSPSFNWKKNLDDATIAKFQQALSDMGYKYQFITLAGIHNMWFNMFDLAYDYARGEGMKHYVEKVQEAEFAAASKGYTF
VAHQQEVGTGYFDKMTNIIQGGASSVTALTGSTEEEQFH
>Mature_438_residues
SKYISRQEQIDALKKDWAENPRWKGVRRPYTAEEVVSLRGSFVPENTVAQRGAAKLWDLVNGGAKKGYVNSLGALTGGQA
VQQAKAGIEAIYLSGWQVAADANLAGTMYPDQSLYPANSVPAVVGRINNSFRRADQIQWSNGVGPEDDKFVDYFLPIVAD
AEAGFGGVLNAYELMKSMIDAGAAGVHFEDQLASVKKCGHMGGKVLVPTQEAVQKLVAARLAADVSGVETLVIARTDANA
ADLLTSDCDPYDSDFITGERTSEGFYRVRAGLDQAIARGLAYAPYADLIWCETAKPDLEEARRFAEAIHAKYPDQLLAYN
CSPSFNWKKNLDDATIAKFQQALSDMGYKYQFITLAGIHNMWFNMFDLAYDYARGEGMKHYVEKVQEAEFAAASKGYTFV
AHQQEVGTGYFDKMTNIIQGGASSVTALTGSTEEEQFH

Specific function: Catalyzes the formation of succinate and glyoxylate from isocitrate, a key step of the glyoxylate cycle. May be involved in the assimilation of one-carbon compounds via the isocitrate lyase- positive serine pathway [H]

COG id: COG2224

COG function: function code C; Isocitrate lyase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate lyase/PEP mutase superfamily. Isocitrate lyase family [H]

Homologues:

Organism=Escherichia coli, GI1790445, Length=433, Percent_Identity=80.6004618937644, Blast_Score=747, Evalue=0.0,
Organism=Escherichia coli, GI1786525, Length=266, Percent_Identity=28.5714285714286, Blast_Score=82, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI17561814, Length=423, Percent_Identity=57.919621749409, Blast_Score=482, Evalue=1e-136,
Organism=Caenorhabditis elegans, GI71982926, Length=423, Percent_Identity=57.919621749409, Blast_Score=482, Evalue=1e-136,
Organism=Saccharomyces cerevisiae, GI6320908, Length=290, Percent_Identity=35.5172413793103, Blast_Score=171, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6325263, Length=256, Percent_Identity=38.28125, Blast_Score=159, Evalue=6e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006254
- InterPro:   IPR000918
- InterPro:   IPR018523
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00463 ICL [H]

EC number: =4.1.3.1 [H]

Molecular weight: Translated: 48029; Mature: 47898

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS00074 GLFV_DEHYDROGENASE ; PS00161 ISOCITRATE_LYASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKYISRQEQIDALKKDWAENPRWKGVRRPYTAEEVVSLRGSFVPENTVAQRGAAKLWDL
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHH
VNGGAKKGYVNSLGALTGGQAVQQAKAGIEAIYLSGWQVAADANLAGTMYPDQSLYPANS
HCCCCCCCHHHHHHHCCCHHHHHHHHCCCEEEEECCEEEEECCCCCCCCCCCCCCCCCCC
VPAVVGRINNSFRRADQIQWSNGVGPEDDKFVDYFLPIVADAEAGFGGVLNAYELMKSMI
CHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
DAGAAGVHFEDQLASVKKCGHMGGKVLVPTQEAVQKLVAARLAADVSGVETLVIARTDAN
HCCCCCCCHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHCCCEEEEEEECCCC
AADLLTSDCDPYDSDFITGERTSEGFYRVRAGLDQAIARGLAYAPYADLIWCETAKPDLE
HHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCHH
EARRFAEAIHAKYPDQLLAYNCSPSFNWKKNLDDATIAKFQQALSDMGYKYQFITLAGIH
HHHHHHHHHHHCCCCHHEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEEHHHH
NMWFNMFDLAYDYARGEGMKHYVEKVQEAEFAAASKGYTFVAHQQEVGTGYFDKMTNIIQ
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEEEHHHHCCCHHHHHHHHHH
GGASSVTALTGSTEEEQFH
CCCCCEEEECCCCCCCCCC
>Mature Secondary Structure 
SKYISRQEQIDALKKDWAENPRWKGVRRPYTAEEVVSLRGSFVPENTVAQRGAAKLWDL
CHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHH
VNGGAKKGYVNSLGALTGGQAVQQAKAGIEAIYLSGWQVAADANLAGTMYPDQSLYPANS
HCCCCCCCHHHHHHHCCCHHHHHHHHCCCEEEEECCEEEEECCCCCCCCCCCCCCCCCCC
VPAVVGRINNSFRRADQIQWSNGVGPEDDKFVDYFLPIVADAEAGFGGVLNAYELMKSMI
CHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
DAGAAGVHFEDQLASVKKCGHMGGKVLVPTQEAVQKLVAARLAADVSGVETLVIARTDAN
HCCCCCCCHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHCCCEEEEEEECCCC
AADLLTSDCDPYDSDFITGERTSEGFYRVRAGLDQAIARGLAYAPYADLIWCETAKPDLE
HHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCHH
EARRFAEAIHAKYPDQLLAYNCSPSFNWKKNLDDATIAKFQQALSDMGYKYQFITLAGIH
HHHHHHHHHHHCCCCHHEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEEHHHH
NMWFNMFDLAYDYARGEGMKHYVEKVQEAEFAAASKGYTFVAHQQEVGTGYFDKMTNIIQ
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEEEHHHHCCCHHHHHHHHHH
GGASSVTALTGSTEEEQFH
CCCCCEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]