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The map label for this gene is ispG

Identifier: 119775499

GI number: 119775499

Start: 2842072

End: 2843190

Strand: Reverse

Name: ispG

Synonym: Sama_2365

Alternate gene names: 119775499

Gene position: 2843190-2842072 (Counterclockwise)

Preceding gene: 119775500

Following gene: 119775498

Centisome position: 66.03

GC content: 54.51

Gene sequence:

>1119_bases
ATGTTCAACGAAAATCCCATCAAGCGTCGCCCCTCTACCCGCATTTACGTCGGTAATGTGCCTATTGGTGACGGCGCGCC
TATTGCTGTCCAGTCCATGACCAACACCCGCACCACAGATGTGGCTGCAACAGTGGCGCAAATTCGGGCACTGGAAAATG
TTGGCGCCGACATAGTACGTGTCTCAGTGCCTACCATGGATGCAGCCGAAGCCTTCAGGCAGATTAAGCAGCAGGTAAAA
GTGCCTCTGGTGGCCGATATCCACTTTGACTACCGTATCGCCCTTAAAGTGGCTGAGTACGGTGTGGATTGTCTGCGTAT
CAACCCGGGTAATATTGGTAACGAAGAGCGCATTCGTGCCGTGGTTGACTGTGCCCGTGACAAAAACATTCCCATCCGTA
TCGGAGTGAATGGCGGCAGTCTCGAAAAAGATCTGATGGACAAGTACAAGGAGCCAACGCCTGAGGCGCTGCTCGAGTCC
GCTATGCGCCATGTGGACATTCTTGACCGCCTCAACTTTGACCAGTTCAAGGTGAGCGTGAAGGCGTCAGACGTTTTCCT
GGCGGTGGAGTCTTACCGACTGCTGGCCAAACAAATCAAACAGCCGCTGCATCTTGGCATCACTGAGGCCGGTGGCGCCC
GCGCCGGTGCGGTGAAATCCGCTGTTGGTCTGGGTATGTTGCTCGCCGAGGGCATTGGCGACACCCTGCGTATTTCCCTG
GCGGCCGATCCGGTTGAAGAAGTGAAAGTGGGTTTTGATATCCTCAAATCACTGCGTATCCGCTCCCGCGGTATCAATTT
TATCGCCTGTCCTTCCTGCTCGCGTCAGGAATTTGATGTTATCTCTACCGTTAACGAGCTTGAGAGACGTCTCGAGGACG
TGACCACGGCAATGGACGTGTCCATCATCGGCTGTGTGGTTAATGGCCCCGGTGAAGCTTTGGTGTCTCATTTGGGGCTG
GCGGGTGGCCACAAGAAGAGTGGTTACTATGACGATGGTATCCGCCAGAAGGAACGTTTTGATAACGATAACCTGGTGGA
TGCACTGGAAGCCAAAATTCGCGCCAAGGCCAGCCTGATGGCAAACCGGATACCCGTGCAAGAGGGTGATGCCGACTAG

Upstream 100 bases:

>100_bases
TGGTGAGGCCGCGAGTCTCGACGAGTTCCCCAAGGGCCGGGTGGCCAGATTCAGTCTGCCCAAAAACGGTTAAGCGCAAC
AGCGTGAGAAGTATTTGACC

Downstream 100 bases:

>100_bases
GAGGCGCTGGTTTTACCTTTGGCCCCCAACCCCCTATAATGCTAACCGCTTTGCCCGGCTCGAACGGGAAGGGCATCTCG
GTTGGCATTAGGTCAACATA

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MFNENPIKRRPSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVAATVAQIRALENVGADIVRVSVPTMDAAEAFRQIKQQVK
VPLVADIHFDYRIALKVAEYGVDCLRINPGNIGNEERIRAVVDCARDKNIPIRIGVNGGSLEKDLMDKYKEPTPEALLES
AMRHVDILDRLNFDQFKVSVKASDVFLAVESYRLLAKQIKQPLHLGITEAGGARAGAVKSAVGLGMLLAEGIGDTLRISL
AADPVEEVKVGFDILKSLRIRSRGINFIACPSCSRQEFDVISTVNELERRLEDVTTAMDVSIIGCVVNGPGEALVSHLGL
AGGHKKSGYYDDGIRQKERFDNDNLVDALEAKIRAKASLMANRIPVQEGDAD

Sequences:

>Translated_372_residues
MFNENPIKRRPSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVAATVAQIRALENVGADIVRVSVPTMDAAEAFRQIKQQVK
VPLVADIHFDYRIALKVAEYGVDCLRINPGNIGNEERIRAVVDCARDKNIPIRIGVNGGSLEKDLMDKYKEPTPEALLES
AMRHVDILDRLNFDQFKVSVKASDVFLAVESYRLLAKQIKQPLHLGITEAGGARAGAVKSAVGLGMLLAEGIGDTLRISL
AADPVEEVKVGFDILKSLRIRSRGINFIACPSCSRQEFDVISTVNELERRLEDVTTAMDVSIIGCVVNGPGEALVSHLGL
AGGHKKSGYYDDGIRQKERFDNDNLVDALEAKIRAKASLMANRIPVQEGDAD
>Mature_372_residues
MFNENPIKRRPSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVAATVAQIRALENVGADIVRVSVPTMDAAEAFRQIKQQVK
VPLVADIHFDYRIALKVAEYGVDCLRINPGNIGNEERIRAVVDCARDKNIPIRIGVNGGSLEKDLMDKYKEPTPEALLES
AMRHVDILDRLNFDQFKVSVKASDVFLAVESYRLLAKQIKQPLHLGITEAGGARAGAVKSAVGLGMLLAEGIGDTLRISL
AADPVEEVKVGFDILKSLRIRSRGINFIACPSCSRQEFDVISTVNELERRLEDVTTAMDVSIIGCVVNGPGEALVSHLGL
AGGHKKSGYYDDGIRQKERFDNDNLVDALEAKIRAKASLMANRIPVQEGDAD

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family

Homologues:

Organism=Escherichia coli, GI1788863, Length=370, Percent_Identity=84.8648648648649, Blast_Score=635, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPG_SHEAM (A1S863)

Other databases:

- EMBL:   CP000507
- RefSeq:   YP_928239.1
- STRING:   A1S863
- GeneID:   4604614
- GenomeReviews:   CP000507_GR
- KEGG:   saz:Sama_2365
- NMPDR:   fig|326297.7.peg.2215
- eggNOG:   COG0821
- HOGENOM:   HBG335271
- OMA:   APIVVQS
- PhylomeDB:   A1S863
- ProtClustDB:   PRK00366
- HAMAP:   MF_00159
- InterPro:   IPR013785
- InterPro:   IPR011005
- InterPro:   IPR016425
- InterPro:   IPR004588
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF004640
- TIGRFAMs:   TIGR00612

Pfam domain/function: PF04551 GcpE; SSF51717 DHP_synth_like

EC number: =1.17.7.1

Molecular weight: Translated: 40501; Mature: 40501

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFNENPIKRRPSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVAATVAQIRALENVGADIVR
CCCCCCCCCCCCCEEEECCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCEEE
VSVPTMDAAEAFRQIKQQVKVPLVADIHFDYRIALKVAEYGVDCLRINPGNIGNEERIRA
EECCCCHHHHHHHHHHHHCCCCEEEEECCCEEEEEEEHHCCCEEEEECCCCCCCHHHHHH
VVDCARDKNIPIRIGVNGGSLEKDLMDKYKEPTPEALLESAMRHVDILDRLNFDQFKVSV
HHHHHCCCCCCEEEECCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEE
KASDVFLAVESYRLLAKQIKQPLHLGITEAGGARAGAVKSAVGLGMLLAEGIGDTLRISL
EECEEEEEHHHHHHHHHHHCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEE
AADPVEEVKVGFDILKSLRIRSRGINFIACPSCSRQEFDVISTVNELERRLEDVTTAMDV
ECCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCE
SIIGCVVNGPGEALVSHLGLAGGHKKSGYYDDGIRQKERFDNDNLVDALEAKIRAKASLM
EEEEEEECCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHH
ANRIPVQEGDAD
HHCCCCCCCCCC
>Mature Secondary Structure
MFNENPIKRRPSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVAATVAQIRALENVGADIVR
CCCCCCCCCCCCCEEEECCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCEEE
VSVPTMDAAEAFRQIKQQVKVPLVADIHFDYRIALKVAEYGVDCLRINPGNIGNEERIRA
EECCCCHHHHHHHHHHHHCCCCEEEEECCCEEEEEEEHHCCCEEEEECCCCCCCHHHHHH
VVDCARDKNIPIRIGVNGGSLEKDLMDKYKEPTPEALLESAMRHVDILDRLNFDQFKVSV
HHHHHCCCCCCEEEECCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEE
KASDVFLAVESYRLLAKQIKQPLHLGITEAGGARAGAVKSAVGLGMLLAEGIGDTLRISL
EECEEEEEHHHHHHHHHHHCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEE
AADPVEEVKVGFDILKSLRIRSRGINFIACPSCSRQEFDVISTVNELERRLEDVTTAMDV
ECCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCE
SIIGCVVNGPGEALVSHLGLAGGHKKSGYYDDGIRQKERFDNDNLVDALEAKIRAKASLM
EEEEEEECCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHH
ANRIPVQEGDAD
HHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA