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The map label for this gene is pleD [H]

Identifier: 119775488

GI number: 119775488

Start: 2826457

End: 2827323

Strand: Direct

Name: pleD [H]

Synonym: Sama_2353

Alternate gene names: 119775488

Gene position: 2826457-2827323 (Clockwise)

Preceding gene: 119775486

Following gene: 119775491

Centisome position: 65.64

GC content: 49.71

Gene sequence:

>867_bases
ATGCATTTTCATAAGCAATCCGGACACTTATACATTCAATATCTTACTCTGATCCTCGGACTCCTTTATGCAGCCTTGTA
TCTTAAGTGGACAGAGCATCAGCCTCAGCTAAAGCACCTTGAATTACTCCTCGAGAGTGTGCCTGTGCTCTTCTGGTTGG
TGATTATGTTCAGCTGCCACTACATACGCCACCTCACCTACAGCTATGTCCTGCTCCTTAGCGGTGCAATCATCTCCTTC
TTTGCCAGCCTGATGGACTGGTTGGATGAAATCACAGTGCCAACAGAATTTGCCTTTGTAGAAGACGTATTGCAGTCAAT
CGGGCTTCTCCTCTCAGCCACCGGCTTATTGCTGCTCTTTCATCATCAGTTAGTGCAGCAAAGTTTGCTTAAAAAAATTG
CCGCCACCGATCCCCTTACCGGCGCCCTGAACCGCCGCGCCTTCAAAGCACCCAAAACGCACAGCGGCAGCACCGTGTTT
GCTCTTGTGGATGTGGACCACTTCAAGCGCATCAATGATACCTATGGCCACGATGCCGGTGACTTTGTGCTGGTTGAGCT
TGCCAAACTTATCAGCAACCATATACGTGAAGATGACCAGTTTTTCCGTTGGGGAGGCGAGGAGTTTTTACTGGAGTTCA
GGCAAGCCGAGTTGGCACAGGCAAGCAAAAGAATGGAAGCCTTGCGCCAACTGGTTGAGTCCAGCCATTTCAATCTCAAT
GGTCAGGCCATCAATATCACCATCAGTGCGGGGCTTACCTGTGTATCACCCAATCATGGAGGCCTCGAAAAGGCGCTTAA
GGCCGCAGATGAAGCGCTTTACCGTGCAAAAGACAGCGGCCGTAACCGAATTGAAGTGGCAGTATAA

Upstream 100 bases:

>100_bases
CCCCAATTTTAATGGGCACAGTATAACTGCCGCCTCCCTTACCTGGCAAAATCTGCGGCTGTGGCTAGACTTTAGTCAGA
CATTTCAAAGGCCGCTGCTA

Downstream 100 bases:

>100_bases
ACGCAGCGTTATTCTGCGCCGCGATTGGGATAGCGATATCAATTCAGCGCCAATGGAAGTGGGGCAATAAACGGCGAACC
ATAGCCATGACTCACATGAA

Product: GGDEF domain-containing protein

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 288; Mature: 288

Protein sequence:

>288_residues
MHFHKQSGHLYIQYLTLILGLLYAALYLKWTEHQPQLKHLELLLESVPVLFWLVIMFSCHYIRHLTYSYVLLLSGAIISF
FASLMDWLDEITVPTEFAFVEDVLQSIGLLLSATGLLLLFHHQLVQQSLLKKIAATDPLTGALNRRAFKAPKTHSGSTVF
ALVDVDHFKRINDTYGHDAGDFVLVELAKLISNHIREDDQFFRWGGEEFLLEFRQAELAQASKRMEALRQLVESSHFNLN
GQAINITISAGLTCVSPNHGGLEKALKAADEALYRAKDSGRNRIEVAV

Sequences:

>Translated_288_residues
MHFHKQSGHLYIQYLTLILGLLYAALYLKWTEHQPQLKHLELLLESVPVLFWLVIMFSCHYIRHLTYSYVLLLSGAIISF
FASLMDWLDEITVPTEFAFVEDVLQSIGLLLSATGLLLLFHHQLVQQSLLKKIAATDPLTGALNRRAFKAPKTHSGSTVF
ALVDVDHFKRINDTYGHDAGDFVLVELAKLISNHIREDDQFFRWGGEEFLLEFRQAELAQASKRMEALRQLVESSHFNLN
GQAINITISAGLTCVSPNHGGLEKALKAADEALYRAKDSGRNRIEVAV
>Mature_288_residues
MHFHKQSGHLYIQYLTLILGLLYAALYLKWTEHQPQLKHLELLLESVPVLFWLVIMFSCHYIRHLTYSYVLLLSGAIISF
FASLMDWLDEITVPTEFAFVEDVLQSIGLLLSATGLLLLFHHQLVQQSLLKKIAATDPLTGALNRRAFKAPKTHSGSTVF
ALVDVDHFKRINDTYGHDAGDFVLVELAKLISNHIREDDQFFRWGGEEFLLEFRQAELAQASKRMEALRQLVESSHFNLN
GQAINITISAGLTCVSPNHGGLEKALKAADEALYRAKDSGRNRIEVAV

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=254, Percent_Identity=28.3464566929134, Blast_Score=101, Evalue=7e-23,
Organism=Escherichia coli, GI145693134, Length=159, Percent_Identity=37.1069182389937, Blast_Score=100, Evalue=8e-23,
Organism=Escherichia coli, GI1786584, Length=225, Percent_Identity=28.8888888888889, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI87081881, Length=184, Percent_Identity=35.8695652173913, Blast_Score=95, Evalue=4e-21,
Organism=Escherichia coli, GI1787816, Length=161, Percent_Identity=34.1614906832298, Blast_Score=88, Evalue=7e-19,
Organism=Escherichia coli, GI87082007, Length=187, Percent_Identity=36.8983957219251, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1788085, Length=179, Percent_Identity=32.9608938547486, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1787802, Length=250, Percent_Identity=27.6, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1788381, Length=187, Percent_Identity=29.9465240641711, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1787056, Length=153, Percent_Identity=35.9477124183007, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI87081974, Length=186, Percent_Identity=32.7956989247312, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI1788956, Length=167, Percent_Identity=32.9341317365269, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI87081977, Length=150, Percent_Identity=30.6666666666667, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1787541, Length=171, Percent_Identity=32.7485380116959, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 32572; Mature: 32572

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHFHKQSGHLYIQYLTLILGLLYAALYLKWTEHQPQLKHLELLLESVPVLFWLVIMFSCH
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
YIRHLTYSYVLLLSGAIISFFASLMDWLDEITVPTEFAFVEDVLQSIGLLLSATGLLLLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
HHQLVQQSLLKKIAATDPLTGALNRRAFKAPKTHSGSTVFALVDVDHFKRINDTYGHDAG
HHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCEEEEEEEHHHHHHHHHHCCCCCC
DFVLVELAKLISNHIREDDQFFRWGGEEFLLEFRQAELAQASKRMEALRQLVESSHFNLN
HHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
GQAINITISAGLTCVSPNHGGLEKALKAADEALYRAKDSGRNRIEVAV
CEEEEEEEECCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEC
>Mature Secondary Structure
MHFHKQSGHLYIQYLTLILGLLYAALYLKWTEHQPQLKHLELLLESVPVLFWLVIMFSCH
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
YIRHLTYSYVLLLSGAIISFFASLMDWLDEITVPTEFAFVEDVLQSIGLLLSATGLLLLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
HHQLVQQSLLKKIAATDPLTGALNRRAFKAPKTHSGSTVFALVDVDHFKRINDTYGHDAG
HHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCEEEEEEEHHHHHHHHHHCCCCCC
DFVLVELAKLISNHIREDDQFFRWGGEEFLLEFRQAELAQASKRMEALRQLVESSHFNLN
HHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
GQAINITISAGLTCVSPNHGGLEKALKAADEALYRAKDSGRNRIEVAV
CEEEEEEEECCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA