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The map label for this gene is flgJ [H]

Identifier: 119775445

GI number: 119775445

Start: 2776246

End: 2777364

Strand: Reverse

Name: flgJ [H]

Synonym: Sama_2310

Alternate gene names: 119775445

Gene position: 2777364-2776246 (Counterclockwise)

Preceding gene: 119775446

Following gene: 119775444

Centisome position: 64.5

GC content: 54.78

Gene sequence:

>1119_bases
ATGGAAAAGCTTTCAAACGCCTCACATTTCCTCGACATCGGTGGCCTGGACAAATTAAGAGCCCAGGCCAAGGCCGATAA
CAAGGCAGCCCTGCGGGAAGTTGCCAAGCAATTCGAGGGGATTTTTGTGCAGATGCTGATGAAAAGCATGCGTGATGCCA
ATGCCGTATTTGAATCCGATAGTCCCTTCAACAGCCAGTACACCAAGTTTTATGAAGAAATGCGCGATCAGCAAATGGCC
GTCGACATGTCCCAAAAAGGCATGCTGGGGCTGGTGGATTTGATGGTGCAACAGCTGTCTCCCAAGGACAGCCCGATAAC
ACCGTCATCAGTACTGGGCGGCGATAAGCGCTTTGATCTGGGGGAGGTCCCCATGATGCTGCGCCGTGGCAAGGACGCTG
AGGCAAAGGCATCACTCGAAGGGATTGAAACGCCTCAGATGGCTGAACGTCAACCTGTCGAGCGGCACAGCGATATGTCC
CTAAACATGGGGTTCCATGAAGCCCCGGTTCTGGATGCACCAACAGCCGCATCGGCTCCTGCTTCCAGCGCCGCAGCACC
TATATTCGACCAATTGCTCGCCGGGAAACAAACGCCCAGTCAGCAGATTGAAAGTGAGCGGGACAATCCGAATTTTGAGA
GCCGTCAGGACTTTATTCGTGCCCTGATGCCACTGGCGAGCGAAGCTGCCAAGCGTCTGGGTACCAAGCCCGAGGTGTTG
ATTGCCCAATCGGCACTGGAAACCGGTTGGGGCCAGAAGCTGGTAAGACGGACGGATGGCAGCATGAGCAATAACCTGTT
CAACATCAAAGCCGACAAGCGCTGGGAAGGGGAAAAAGCGGCAGTCAATACCCTGGAATTTGAGCAGGGCGTTGCGGTAC
GGCAACGAGCTGACTTCCGGGTTTACGACACCCTGGAGCAGAGCTTCAACGATTTTGTCGATTTCCTGAAAAGCGGCGAG
CGCTACGGTGATGCCCTGAAACAAGCGGCGAGGCCTGCATCCTTTGTGCAGGGGTTACAAAATGCCGGGTATGCCACAGA
CCCTCAATATGCCGACAAGGTGATGCGGGTAATGCAGGCGGTAACGTCTGAAATCAAGGCCATGGCCCTTGGCAACTGA

Upstream 100 bases:

>100_bases
TTCAGATGTGCTTGCGATCCTTGAGGCGTTAAAAATGGCAGGCGCCTTGCATGGTGAGCTTATCGTCATTTGATTCTTGT
CGGAAAATTGGCAACTCACC

Downstream 100 bases:

>100_bases
CCCTGACAGGCGGAGAAAGAAATGAGCATAGATTTATTGAACATCGCCCGGACCGGGGTACTGGCATCTCAGGCACAGCT
CGGGGTGACCAGTAACAACA

Product: flagellar rod assembly protein/muramidase FlgJ

Products: NA

Alternate protein names: Muramidase flgJ [H]

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MEKLSNASHFLDIGGLDKLRAQAKADNKAALREVAKQFEGIFVQMLMKSMRDANAVFESDSPFNSQYTKFYEEMRDQQMA
VDMSQKGMLGLVDLMVQQLSPKDSPITPSSVLGGDKRFDLGEVPMMLRRGKDAEAKASLEGIETPQMAERQPVERHSDMS
LNMGFHEAPVLDAPTAASAPASSAAAPIFDQLLAGKQTPSQQIESERDNPNFESRQDFIRALMPLASEAAKRLGTKPEVL
IAQSALETGWGQKLVRRTDGSMSNNLFNIKADKRWEGEKAAVNTLEFEQGVAVRQRADFRVYDTLEQSFNDFVDFLKSGE
RYGDALKQAARPASFVQGLQNAGYATDPQYADKVMRVMQAVTSEIKAMALGN

Sequences:

>Translated_372_residues
MEKLSNASHFLDIGGLDKLRAQAKADNKAALREVAKQFEGIFVQMLMKSMRDANAVFESDSPFNSQYTKFYEEMRDQQMA
VDMSQKGMLGLVDLMVQQLSPKDSPITPSSVLGGDKRFDLGEVPMMLRRGKDAEAKASLEGIETPQMAERQPVERHSDMS
LNMGFHEAPVLDAPTAASAPASSAAAPIFDQLLAGKQTPSQQIESERDNPNFESRQDFIRALMPLASEAAKRLGTKPEVL
IAQSALETGWGQKLVRRTDGSMSNNLFNIKADKRWEGEKAAVNTLEFEQGVAVRQRADFRVYDTLEQSFNDFVDFLKSGE
RYGDALKQAARPASFVQGLQNAGYATDPQYADKVMRVMQAVTSEIKAMALGN
>Mature_372_residues
MEKLSNASHFLDIGGLDKLRAQAKADNKAALREVAKQFEGIFVQMLMKSMRDANAVFESDSPFNSQYTKFYEEMRDQQMA
VDMSQKGMLGLVDLMVQQLSPKDSPITPSSVLGGDKRFDLGEVPMMLRRGKDAEAKASLEGIETPQMAERQPVERHSDMS
LNMGFHEAPVLDAPTAASAPASSAAAPIFDQLLAGKQTPSQQIESERDNPNFESRQDFIRALMPLASEAAKRLGTKPEVL
IAQSALETGWGQKLVRRTDGSMSNNLFNIKADKRWEGEKAAVNTLEFEQGVAVRQRADFRVYDTLEQSFNDFVDFLKSGE
RYGDALKQAARPASFVQGLQNAGYATDPQYADKVMRVMQAVTSEIKAMALGN

Specific function: Flagellum-specific muramidase which hydrolyzes the peptidoglycan layer to assemble the rod structure in the periplasmic space [H]

COG id: COG1705

COG function: function code NU; Muramidase (flagellum-specific)

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the glycosyl hydrolase 73 family [H]

Homologues:

Organism=Escherichia coli, GI1787321, Length=359, Percent_Identity=31.7548746518106, Blast_Score=153, Evalue=2e-38,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013377
- InterPro:   IPR000423
- InterPro:   IPR019301
- InterPro:   IPR013338
- InterPro:   IPR002901 [H]

Pfam domain/function: PF01832 Glucosaminidase; PF10135 Rod-binding [H]

EC number: 3.2.1.-

Molecular weight: Translated: 41120; Mature: 41120

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKLSNASHFLDIGGLDKLRAQAKADNKAALREVAKQFEGIFVQMLMKSMRDANAVFESD
CCCCCCCHHEEECCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SPFNSQYTKFYEEMRDQQMAVDMSQKGMLGLVDLMVQQLSPKDSPITPSSVLGGDKRFDL
CCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCH
GEVPMMLRRGKDAEAKASLEGIETPQMAERQPVERHSDMSLNMGFHEAPVLDAPTAASAP
HHHHHHHHCCCCCHHHHHHHCCCCCHHHHCCCHHHHCCCEECCCCCCCCCCCCCCCCCCC
ASSAAAPIFDQLLAGKQTPSQQIESERDNPNFESRQDFIRALMPLASEAAKRLGTKPEVL
CCCCHHHHHHHHHCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
IAQSALETGWGQKLVRRTDGSMSNNLFNIKADKRWEGEKAAVNTLEFEQGVAVRQRADFR
HHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHCHHHHHHCCCH
VYDTLEQSFNDFVDFLKSGERYGDALKQAARPASFVQGLQNAGYATDPQYADKVMRVMQA
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
VTSEIKAMALGN
HHHHHHHHHCCC
>Mature Secondary Structure
MEKLSNASHFLDIGGLDKLRAQAKADNKAALREVAKQFEGIFVQMLMKSMRDANAVFESD
CCCCCCCHHEEECCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SPFNSQYTKFYEEMRDQQMAVDMSQKGMLGLVDLMVQQLSPKDSPITPSSVLGGDKRFDL
CCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCH
GEVPMMLRRGKDAEAKASLEGIETPQMAERQPVERHSDMSLNMGFHEAPVLDAPTAASAP
HHHHHHHHCCCCCHHHHHHHCCCCCHHHHCCCHHHHCCCEECCCCCCCCCCCCCCCCCCC
ASSAAAPIFDQLLAGKQTPSQQIESERDNPNFESRQDFIRALMPLASEAAKRLGTKPEVL
CCCCHHHHHHHHHCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
IAQSALETGWGQKLVRRTDGSMSNNLFNIKADKRWEGEKAAVNTLEFEQGVAVRQRADFR
HHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHCHHHHHHCCCH
VYDTLEQSFNDFVDFLKSGERYGDALKQAARPASFVQGLQNAGYATDPQYADKVMRVMQA
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
VTSEIKAMALGN
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9742694; 10952301 [H]